Status
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Gene information
General information
TC030595 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1029123..1032210
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030595
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06034

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030595 RA
      MGKLQHSES EEENDSDYG EDMLEMVEE DDLDFLKNA 
      ITSKSYNIF NKVKYSGAP KPKRRRLNN EDSDLEKEF 
      EEQTVEDTP MKNLLPIKT KFGIVPQQI EDESGEKSE 
      DEAEEDETQ EQFDLVDDK IDISKPIPA AHLLAERQK 
      VLREKKIHI GTLSAGVLE NPEEKVTNL RTLLKLMDE 
      ESSEVFFTV RKLATVSLL EVFKDVLPS YEIKKVNND 
      GVKLKKDTL KLQKYEESL VQYYKKFLQ KLEKNSSVL 
      LKKKGDTRK FHEEEIKLA ELAVQALCD LLVAHPYFN 
      YAQNIAQAV VPFLNNVRS NIREIAKNA IRTVFKEDK 
      REEIILKIL RIINAYLKN RAHNVHPDM LETFLVLNL 
      RDVNLDEEK EQDIKQKKL MARKQKVLQ MSKKERKRK 
      KKLQMLEQE LLETKAEEN KQSKQRNLT EITKIVFGV 
      YFRILKSST NNKVLGVCL EGLAKFSHC INIEYYLDL 
      VNILDNLLK EEWIGYREQ LHCVQTVFS ILSGQGEAL 
      NVDPTRFYT NLYKGLLTT NASKNHSNF LILLKTLND 
      ALIKRRKKI TNKRTLSFV KRLATLSLQ LLHNGSLGS 
      LGLIKNIMQ LNRTVDILL DLDNSFGDG KYQPELEDP 
      EYANASSTG LYELNLLVR HYHPVVTKY ARNIAFGVP 
      ATGEGSLDP EYAKCTPEQ LYHDFDMCE MAFNPPVPV 
      PKKVAPKEK HQKHLFADS AFESQCKSI VRAHLKRKA 
      SFL
    CDS sequence
    • >TC030595 RA
      atgggaaaa ttgcaacat tccgaaagt gaggaggaa 
      aatgatagt gattatggg gaggacatg cttgaaatg 
      gtcgaagag gacgacttg gacttcctc aaaaatgcc 
      ataacttca aaatcgtac aacattttc aacaaagtc 
      aagtattcc ggtgcccct aaacccaaa aggcgccgc 
      ttaaataat gaagactcg gatctggag aaagagttt 
      gaggagcaa actgttgaa gacaccccc atgaaaaat 
      ctcctaccc atcaaaact aagtttgga attgtgccg 
      caacaaatt gaggacgag tccggcgag aaatctgaa 
      gatgaagcc gaagaagac gaaacgcag gagcagttt 
      gacctagtt gacgataaa attgatatt tcgaaacca 
      attcctgcg gcacattta ttggccgag cgacagaaa 
      gttttaaga gagaaaaaa attcacatt gggactttg 
      agtgccggt gttttggaa aatcccgaa gaaaaagta 
      acaaatcta cgaacttta ctcaaacta atggatgag 
      gaaagtagc gaagtgttt ttcactgtg aggaaattg 
      gccactgtg tcgctccta gaagtgttc aaagacgtt 
      ttgccttca tatgaaatc aaaaaagtc aacaatgat 
      ggagtgaaa ttgaaaaag gatacatta aaattacaa 
      aaatatgaa gaaagttta gtgcaatat tacaaaaag 
      tttttgcaa aagcttgaa aagaactct tcggttttg 
      ctcaagaaa aaaggtgac acaagaaaa tttcacgaa 
      gaagaaatt aaattggct gaactagca gtgcaggca 
      ttatgtgac ttactagtt gcacaccct tatttcaat 
      tatgcccaa aatatagca caagctgtt gttcccttc 
      ttaaataac gtaagaagt aacatacgg gaaattgca 
      aaaaatgca ataagaaca gttttcaag gaggataaa 
      agggaagaa atcatacta aagatttta cgaataatc 
      aacgcctat ttaaaaaac cgagctcac aatgttcat 
      cccgacatg ttagagaca ttccttgtt cttaattta 
      cgagacgtt aatttagac gaggaaaag gagcaagac 
      attaaacag aaaaaattg atggcacgc aaacaaaaa 
      gtactacaa atgtccaaa aaagagcgc aaacgtaag 
      aaaaaactt caaatgttg gaacaggaa ttactagaa 
      actaaagct gaagagaac aaacagtca aaacagcgc 
      aacttgact gaaatcaca aaaatagtt ttcggggtt 
      tatttcaga attttgaaa agttcgacc aataataaa 
      gttctgggc gtttgtcta gaaggcctg gcaaagttt 
      tctcattgc ataaatata gaatattac ttagacctt 
      gtcaacatt ttggacaat ttattgaaa gaagagtgg 
      atcggttac agagagcag ttacattgc gtccaaact 
      gttttttct attctgagc ggacaagga gaagcttta 
      aatgtagac ccgactcgt ttttacacc aatttatac 
      aaaggtctc ctcacaacc aacgcctct aaaaaccac 
      tcaaatttt cttattctt ttgaaaaca ctcaacgat 
      gctctgatc aaaagacgc aaaaaaata acaaataaa 
      aggacgctc agtttcgtg aaacgtttg gccactctt 
      agcttacaa ttgttgcat aatggtagt ttagggagc 
      cttggtctt atcaaaaac ataatgcag cttaacaga 
      acagtggat atcctccta gacttggat aattcgttc 
      ggagacgga aaatatcag ccagaactg gaagaccct 
      gaatatgcg aacgcgtca agtaccggt ttatacgaa 
      ctgaatttg ttggtgaga cattaccat cctgttgtc 
      accaaatac gccagaaat atcgctttt ggggtcccc 
      gccacgggg gaaggcagc cttgacccc gaatacgcc 
      aaatgcaca cctgagcaa ctctaccac gattttgac 
      atgtgtgaa atggccttc aacccccct gtccctgtc 
      cccaaaaaa gtggcccct aaggagaag caccaaaag 
      cacctattt gccgattcg gccttcgag agccagtgc 
      aaaagtata gttagggca catttgaaa cgcaaagcg 
      agttttttg taa
    mRNA sequence
    • >TC030595 RA
      tactttttc tactttgaa attctccag ttctggatt 
      ggaataaac aacgattga ggttaggtg ggattataa 
      aaacgtgtt taaatttgc aaaaaaaaa taaaaaaat 
      gggaaaatt gcaacattc cgaaagtga ggaggaaaa 
      tgatagtga ttatgggga ggacatgct tgaaatggt 
      cgaagagga cgacttgga cttcctcaa aaatgccat 
      aacttcaaa atcgtacaa cattttcaa caaagtcaa 
      gtattccgg tgcccctaa acccaaaag gcgccgctt 
      aaataatga agactcgga tctggagaa agagtttga 
      ggagcaaac tgttgaaga cacccccat gaaaaatct 
      cctacccat caaaactaa gtttggaat tgtgccgca 
      acaaattga ggacgagtc cggcgagaa atctgaaga 
      tgaagccga agaagacga aacgcagga gcagtttga 
      cctagttga cgataaaat tgatatttc gaaaccaat 
      tcctgcggc acatttatt ggccgagcg acagaaagt 
      tttaagaga gaaaaaaat tcacattgg gactttgag 
      tgccggtgt tttggaaaa tcccgaaga aaaagtaac 
      aaatctacg aactttact caaactaat ggatgagga 
      aagtagcga agtgttttt cactgtgag gaaattggc 
      cactgtgtc gctcctaga agtgttcaa agacgtttt 
      gccttcata tgaaatcaa aaaagtcaa caatgatgg 
      agtgaaatt gaaaaagga tacattaaa attacaaaa 
      atatgaaga aagtttagt gcaatatta caaaaagtt 
      tttgcaaaa gcttgaaaa gaactcttc ggttttgct 
      caagaaaaa aggtgacac aagaaaatt tcacgaaga 
      agaaattaa attggctga actagcagt gcaggcatt 
      atgtgactt actagttgc acaccctta tttcaatta 
      tgcccaaaa tatagcaca agctgttgt tcccttctt 
      aaataacgt aagaagtaa catacggga aattgcaaa 
      aaatgcaat aagaacagt tttcaagga ggataaaag 
      ggaagaaat catactaaa gattttacg aataatcaa 
      cgcctattt aaaaaaccg agctcacaa tgttcatcc 
      cgacatgtt agagacatt ccttgttct taatttacg 
      agacgttaa tttagacga ggaaaagga gcaagacat 
      taaacagaa aaaattgat ggcacgcaa acaaaaagt 
      actacaaat gtccaaaaa agagcgcaa acgtaagaa 
      aaaacttca aatgttgga acaggaatt actagaaac 
      taaagctga agagaacaa acagtcaaa acagcgcaa 
      cttgactga aatcacaaa aatagtttt cggggttta 
      tttcagaat tttgaaaag ttcgaccaa taataaagt 
      tctgggcgt ttgtctaga aggcctggc aaagttttc 
      tcattgcat aaatataga atattactt agaccttgt 
      caacatttt ggacaattt attgaaaga agagtggat 
      cggttacag agagcagtt acattgcgt ccaaactgt 
      tttttctat tctgagcgg acaaggaga agctttaaa 
      tgtagaccc gactcgttt ttacaccaa tttatacaa 
      aggtctcct cacaaccaa cgcctctaa aaaccactc 
      aaattttct tattctttt gaaaacact caacgatgc 
      tctgatcaa aagacgcaa aaaaataac aaataaaag 
      gacgctcag tttcgtgaa acgtttggc cactcttag 
      cttacaatt gttgcataa tggtagttt agggagcct 
      tggtcttat caaaaacat aatgcagct taacagaac 
      agtggatat cctcctaga cttggataa ttcgttcgg 
      agacggaaa atatcagcc agaactgga agaccctga 
      atatgcgaa cgcgtcaag taccggttt atacgaact 
      gaatttgtt ggtgagaca ttaccatcc tgttgtcac 
      caaatacgc cagaaatat cgcttttgg ggtccccgc 
      cacggggga aggcagcct tgaccccga atacgccaa 
      atgcacacc tgagcaact ctaccacga ttttgacat 
      gtgtgaaat ggccttcaa cccccctgt ccctgtccc 
      caaaaaagt ggcccctaa ggagaagca ccaaaagca 
      cctatttgc cgattcggc cttcgagag ccagtgcaa 
      aagtatagt tagggcaca tttgaaacg caaagcgag 
      ttttttgta aaataaaag ataaatttt ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY