Status
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Gene information
General information
TC030588 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5462153..5472001
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030588
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030588 RA
      MAKKVSGDS NEMREFAAR ICRDYLYGP WKSVTAQNI 
      GFKHISGGL SNLLYHISL PESLVEESK DLGEPQEVL 
      IRVYGQTHG EHALEALIT ESVVFTLLS ERGLGPKLH 
      GIFPGGRIE QYINARPLR TGELADEKL SVKIAQKMA 
      AIHTMEVPL HKEPGWLWD TIDRWLRTC ESKLKSDDV 
      PEFFRNSVD CDFLSEVDW LKKRLEMEN CPVVFCHND 
      MQEGNILIR QDGPENNNN EDPQIVVID FEYCSYNYR 
      SFDIANHFV EWVYDYTEA EYPFYKEQL ENYPSKKQR 
      LLFIKAYLE ARGLKENPK KILREVEVF TLASHFFWG 
      IWGIINAGT SQIPFGYWE YAAARLRNY FQQKKKFTL 
      DMKPLVSSL PTKRKSCSL E
    • >TC030588 RB
      MAKKVSGDS NEMREFAAR ICRDYLYGP WKSVTAQNI 
      GFKHISGGL SNLLYHISL PESLVEESK DLGEPQEVL 
      IRVYGQTHG EHALEALIT ESVVFTLLS ERGLGPKLH 
      GIFPGGRIE QYINARPLR TGELADEKL SVKIAQKMA 
      AIHTMEVTF FKSGC
    • >TC030588 RC
      MSCSLVPLH KEPGWLWDT IDRWLRTCE SKLKSDDVP 
      EFFRNSVDC DFLSEVDWL KKRLEMENC PVVFCHNDM 
      QEGNILIRQ DGPENNNNE DPQIVVIDF EYCSYNYRS 
      FDIANHFVE WVYDYTEAE YPFYKEQLE NYPSKKQRL 
      LFIKAYLEA RGLKENPKK ILREVEVFT LASHFFWGI 
      WGIINAGTS QIPFGYWEY AAARLRNYF QQKKKFTLD 
      MKPLVSSLP TKRKSCSLE
    CDS sequence
    • >TC030588 RA
      atggcaaaa aaggtgtcg ggtgactcg aacgaaatg 
      agggaattc gctgcgaga atatgcagg gattatctc 
      tatgggcca tggaaaagc gtcactgca caaaacatt 
      ggcttcaaa catatcagc ggcggcttg tccaacctc 
      ctgtaccac atatccttg ccggaatcc ctcgtagaa 
      gagagcaag gacttgggc gaaccccag gaagtcctg 
      atccgcgtc tacggccaa acccatggc gagcatgcc 
      cttgaggcc ctcatcacc gaaagcgtc gtcttcaca 
      ttactgagc gaaagaggt ctcggccca aaactccac 
      gggattttt ccaggaggc cgtatcgag cagtacatc 
      aatgccaga ccattacgt accggagaa ctcgccgac 
      gagaagttg tcggtaaag attgcacag aaaatggcc 
      gccatacat acaatggag gtgcccctg cacaaggag 
      cccggttgg ctctgggac accatcgat cgatggttg 
      aggacgtgc gaatcgaag ctgaaaagt gatgatgtt 
      cctgaattt ttccgaaac agtgtcgat tgcgacttt 
      ttgtcggaa gtcgattgg ctgaaaaaa cggctcgag 
      atggagaat tgtccggtc gttttttgc cacaacgac 
      atgcaagag ggcaatatt ttgatcaga caagacgga 
      cccgagaat aataataac gaggatccg cagatcgtc 
      gtcatcgat ttcgaatac tgctcatac aactaccgt 
      tcgttcgac atcgccaat catttcgtc gaatgggtg 
      tacgactac accgaagcg gaatacccc ttttataaa 
      gaacaactc gaaaattac ccatcgaag aaacaacgg 
      ctcctattc atcaaagcc tacttagaa gcgcgcggt 
      ttgaaagaa aaccccaag aaaattcta cgcgaagtg 
      gaagtcttc actcttgcc tctcatttc ttttggggg 
      atttgggga attatcaac gcaggcaca tcccaaatc 
      ccgttcggt tactgggaa tatgccgca gcccgtttg 
      agaaattac ttccaacag aaaaagaag ttcacttta 
      gatatgaaa ccgctagtg tcgtcactt ccgactaaa 
      cgaaagagt tgcagtctg gagtga
    • >TC030588 RB
      atggcaaaa aaggtgtcg ggtgactcg aacgaaatg 
      agggaattc gctgcgaga atatgcagg gattatctc 
      tatgggcca tggaaaagc gtcactgca caaaacatt 
      ggcttcaaa catatcagc ggcggcttg tccaacctc 
      ctgtaccac atatccttg ccggaatcc ctcgtagaa 
      gagagcaag gacttgggc gaaccccag gaagtcctg 
      atccgcgtc tacggccaa acccatggc gagcatgcc 
      cttgaggcc ctcatcacc gaaagcgtc gtcttcaca 
      ttactgagc gaaagaggt ctcggccca aaactccac 
      gggattttt ccaggaggc cgtatcgag cagtacatc 
      aatgccaga ccattacgt accggagaa ctcgccgac 
      gagaagttg tcggtaaag attgcacag aaaatggcc 
      gccatacat acaatggag gtaacattt tttaagtca 
      ggttgttga
    • >TC030588 RC
      atgtcttgc agtttagtg cccctgcac aaggagccc 
      ggttggctc tgggacacc atcgatcga tggttgagg 
      acgtgcgaa tcgaagctg aaaagtgat gatgttcct 
      gaatttttc cgaaacagt gtcgattgc gactttttg 
      tcggaagtc gattggctg aaaaaacgg ctcgagatg 
      gagaattgt ccggtcgtt ttttgccac aacgacatg 
      caagagggc aatattttg atcagacaa gacggaccc 
      gagaataat aataacgag gatccgcag atcgtcgtc 
      atcgatttc gaatactgc tcatacaac taccgttcg 
      ttcgacatc gccaatcat ttcgtcgaa tgggtgtac 
      gactacacc gaagcggaa taccccttt tataaagaa 
      caactcgaa aattaccca tcgaagaaa caacggctc 
      ctattcatc aaagcctac ttagaagcg cgcggtttg 
      aaagaaaac cccaagaaa attctacgc gaagtggaa 
      gtcttcact cttgcctct catttcttt tgggggatt 
      tggggaatt atcaacgca ggcacatcc caaatcccg 
      ttcggttac tgggaatat gccgcagcc cgtttgaga 
      aattacttc caacagaaa aagaagttc actttagat 
      atgaaaccg ctagtgtcg tcacttccg actaaacga 
      aagagttgc agtctggag tga
    mRNA sequence
    • >TC030588 RA
      attgccccc tctgtccac ctcagataa aatttttat 
      ctttatagt atacaccag tttctctcg agcactcat 
      cagtctcgc ttcgaccaa agtaggact actttagtg 
      tctcaataa tccacaaat ttcatttca gtgctgtct 
      catttcggt gcaataaag ccctcagcg ttcaaaaag 
      catggcaaa aaaggtgtc gggtgactc gaacgaaat 
      gagggaatt cgctgcgag aatatgcag ggattatct 
      ctatgggcc atggaaaag cgtcactgc acaaaacat 
      tggcttcaa acatatcag cggcggctt gtccaacct 
      cctgtacca catatcctt gccggaatc cctcgtaga 
      agagagcaa ggacttggg cgaacccca ggaagtcct 
      gatccgcgt ctacggcca aacccatgg cgagcatgc 
      ccttgaggc cctcatcac cgaaagcgt cgtcttcac 
      attactgag cgaaagagg tctcggccc aaaactcca 
      cgggatttt tccaggagg ccgtatcga gcagtacat 
      caatgccag accattacg taccggaga actcgccga 
      cgagaagtt gtcggtaaa gattgcaca gaaaatggc 
      cgccataca tacaatgga ggtgcccct gcacaagga 
      gcccggttg gctctggga caccatcga tcgatggtt 
      gaggacgtg cgaatcgaa gctgaaaag tgatgatgt 
      tcctgaatt tttccgaaa cagtgtcga ttgcgactt 
      tttgtcgga agtcgattg gctgaaaaa acggctcga 
      gatggagaa ttgtccggt cgttttttg ccacaacga 
      catgcaaga gggcaatat tttgatcag acaagacgg 
      acccgagaa taataataa cgaggatcc gcagatcgt 
      cgtcatcga tttcgaata ctgctcata caactaccg 
      ttcgttcga catcgccaa tcatttcgt cgaatgggt 
      gtacgacta caccgaagc ggaataccc cttttataa 
      agaacaact cgaaaatta cccatcgaa gaaacaacg 
      gctcctatt catcaaagc ctacttaga agcgcgcgg 
      tttgaaaga aaaccccaa gaaaattct acgcgaagt 
      ggaagtctt cactcttgc ctctcattt cttttgggg 
      gatttgggg aattatcaa cgcaggcac atcccaaat 
      cccgttcgg ttactggga atatgccgc agcccgttt 
      gagaaatta cttccaaca gaaaaagaa gttcacttt 
      agatatgaa accgctagt gtcgtcact tccgactaa 
      acgaaagag ttgcagtct ggagtgaaa tcgtatact 
      tccggttaa ctttcgact agtcgaggt tgctaacta 
      aaggctgat gattttaca cgtgatatt agatatcaa 
      aaagtgtgg caagtgtac ctaagatga agctcgtga 
      ttggttagg tattcgtaa tttatcatc atcacattt 
      tattacgca cgaaatttt attattcgc acagaaatt 
      atttatatt tacaaaatt gaagctaag ccgagggct 
      atctcgttt ggttaagtt tgttggttg ttggaatgt 
      acttaatat tcgagactg agcgctatg ttcgaattt 
      taaatgcat ttaaatgtt acgtttctc aaactatat 
      gtgtatata agttattcg atatgttca attaagatc 
      attctatct tttgtaaat aatttattt taataaaca 
      tgaagtccc aag
    • >TC030588 RB
      attgccccc tctgtccac ctcagataa aatttttat 
      ctttatagt atacaccag tttctctcg agcactcat 
      cagtctcgc ttcgaccaa atgctgtct catttcggt 
      gcaataaag ccctcagcg ttcaaaaag catggcaaa 
      aaaggtgtc gggtgactc gaacgaaat gagggaatt 
      cgctgcgag aatatgcag ggattatct ctatgggcc 
      atggaaaag cgtcactgc acaaaacat tggcttcaa 
      acatatcag cggcggctt gtccaacct cctgtacca 
      catatcctt gccggaatc cctcgtaga agagagcaa 
      ggacttggg cgaacccca ggaagtcct gatccgcgt 
      ctacggcca aacccatgg cgagcatgc ccttgaggc 
      cctcatcac cgaaagcgt cgtcttcac attactgag 
      cgaaagagg tctcggccc aaaactcca cgggatttt 
      tccaggagg ccgtatcga gcagtacat caatgccag 
      accattacg taccggaga actcgccga cgagaagtt 
      gtcggtaaa gattgcaca gaaaatggc cgccataca 
      tacaatgga ggtaacatt ttttaagtc aggttgttg 
      aattattct gttttattt agaggcggc gttgcaccc 
      atgccatgg gtttgtgtg aacgggtcg tgtgattaa 
      gtaggtctt ggaatttgg attttttcc ttacgactt 
      tgtggaaaa ttacggatg tatttatct cattcggtg 
      tcaatattt acgacaatg catttattt gtagtttct 
      ttgagattt ctttaagga cacgtggtg tatcaccac 
      atttgacat aatttgaat tcctcaagg tcacgatat 
      tgcgtttca gtcggcgct gcacggcga cctttccct 
      gataaatat tcgcaactt atttcgcac tatgataag 
      ggggcgtta cccgataaa accaaaaaa aatgtattt 
      cctgttgtg attcaatta taaattgtg ataaaaaag 
      tagagcatt tgagacaat aaaactgac aaatattta 
      ccaagttac ttgactctg cctcaaggt gattttttc 
      gctcattct agaaattat ttcatattt atagaacta 
      atcaatatg caagagaaa ggctgctaa tgcaaaaca 
      actaactgg aatagtaag ctgtgataa aaaattgac 
      gtctgttat tatcacagc ctgctacag taattgttt 
      caggataag ttatcgtgg tatgctgtt atcgatttt 
      ttcaacgca actgtgtga ctgtttcac atatctggt 
      tatttccgg aattgattt tgtgataaa attgaatat 
      ttaaacact tcaaaaatt gcgtctttg acaaaatcg 
      cggttgctt aacaaattt ttgcaccga gtggcgaaa 
      tcacgtcct acttttgtg cacaatgtt tgcattaaa 
      attaaaaag tgtgacccc ttttgaaaa attatgtgg 
      gacgcctat gtctcaaac gtgaagtta gccattacc 
      aatggcggc tatgttcac accgctgat tttccaaat 
      ttattggaa aaatcctca ttttcgcgt aacacaagc 
      cgaaaacac gaaaaacat gcaaacgga accaaaaat 
      aaacatttc acaatcaat ttcaataga atctgacct 
      ttcatccaa ccggcgaca tcccggact tgattgtca 
      caatttttc acggatgca cttcaattt cagtctaat 
      tttatcaaa cgggacacg caacagttg gaaatgcgt 
      cacttttac gttttccgg cactttttt cgtgaaaat 
      tgcagagaa aatcggcgg actttcggt caagttttc 
      gcggacttt gccaacatt acgtaaaca aaaaccctg 
      tcaaatttt gattttaat taaaaaaac aagttttag 
      tgaactgtt ttaattata aatcgactc atgaccctt 
      gcgggtttg cgtttgtgc ctgaaatta attttgaga 
      aggtggaac atggaaaat taactgcca accgtagca 
      acaaaaaag tggcgctaa atttaaatt tgaatgaat 
      tcggtgaat tacataatg ggttattat tgcgtcttt 
      tgtctgatt ttcaaggaa gtagcagct gataaagct 
      atttactgg ctagcagat caacctggt cgaggttat 
      cacaatttc ttgcactta aaccctgat aaagcaatt 
      gtcattgga tttcgctcg agcgctcac acaaacaag 
      aataaatgt ggttgtttt ag
    • >TC030588 RC
      tggggattt gttgttgtt atgttctag cgttgtcct 
      atcgtggac ccaaacatc tcgaatcac tctcacgtg 
      ccttcctga ggcgcaaaa caacagccg tttctgtta 
      aaaaacgcc atttgggga cgtccaacc gtaggaatc 
      attcatatg actctaaaa aaataactc aaaattttg 
      accaaaaaa aaatgttcc gagagtgac atgtttttt 
      gtccgagac gaggagtga cgtagaagt acgtctagg 
      ctttgcttg gctaatttt tacccattc atttgcttt 
      cttacaatc attcatcgc ttttggttt gttcgactc 
      actttgaca accacttga ttttagcca atctggtaa 
      gtcccacaa ctgagtcaa acaaaccaa aatatcgta 
      ttatttttg ggaaaacag ttggccgac aggaaacat 
      gtcttgcag tttagtgcc cctgcacaa ggagcccgg 
      ttggctctg ggacaccat cgatcgatg gttgaggac 
      gtgcgaatc gaagctgaa aagtgatga tgttcctga 
      atttttccg aaacagtgt cgattgcga ctttttgtc 
      ggaagtcga ttggctgaa aaaacggct cgagatgga 
      gaattgtcc ggtcgtttt ttgccacaa cgacatgca 
      agagggcaa tattttgat cagacaaga cggacccga 
      gaataataa taacgagga tccgcagat cgtcgtcat 
      cgatttcga atactgctc atacaacta ccgttcgtt 
      cgacatcgc caatcattt cgtcgaatg ggtgtacga 
      ctacaccga agcggaata cccctttta taaagaaca 
      actcgaaaa ttacccatc gaagaaaca acggctcct 
      attcatcaa agcctactt agaagcgcg cggtttgaa 
      agaaaaccc caagaaaat tctacgcga agtggaagt 
      cttcactct tgcctctca tttcttttg ggggatttg 
      gggaattat caacgcagg cacatccca aatcccgtt 
      cggttactg ggaatatgc cgcagcccg tttgagaaa 
      ttacttcca acagaaaaa gaagttcac tttagatat 
      gaaaccgct agtgtcgtc acttccgac taaacgaaa 
      gagttgcag tctggagtg aaatcgtat acttccggt 
      taactttcg actagtcga ggttgctaa ctaaaggct 
      gatgatttt acacgtgat attagatat caaaaagtg 
      tggcaagtg tacctaaga tgaagctcg tgattggtt 
      aggtattcg taatttatc atcatcaca ttttattac 
      gcacgaaat tttattatt cgcacagaa attatttat 
      atttacaaa attgaagct aagccgagg gctatctcg 
      tttggttaa gtttgttgg ttgttggaa tgtacttaa 
      tattcgaga ctgagcgct atgttcgaa ttttaaatg 
      catttaaat gttacgttt ctcaaacta tatgtgtat 
      ataagttat tcgatatgt tcaattaag atcattcta 
      tcttttgta aataattta ttttaataa acatgaagt 
      cccaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY