| General information | |||
| TC030588 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5462153..5472001 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030588 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06033 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
tropharium structure irregular - Number of animals showing the phenotype: 4
previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC030588 RA
MAKKVSGDS NEMREFAAR ICRDYLYGP WKSVTAQNI GFKHISGGL SNLLYHISL PESLVEESK DLGEPQEVL IRVYGQTHG EHALEALIT ESVVFTLLS ERGLGPKLH GIFPGGRIE QYINARPLR TGELADEKL SVKIAQKMA AIHTMEVPL HKEPGWLWD TIDRWLRTC ESKLKSDDV PEFFRNSVD CDFLSEVDW LKKRLEMEN CPVVFCHND MQEGNILIR QDGPENNNN EDPQIVVID FEYCSYNYR SFDIANHFV EWVYDYTEA EYPFYKEQL ENYPSKKQR LLFIKAYLE ARGLKENPK KILREVEVF TLASHFFWG IWGIINAGT SQIPFGYWE YAAARLRNY FQQKKKFTL DMKPLVSSL PTKRKSCSL E
- >TC030588 RB
MAKKVSGDS NEMREFAAR ICRDYLYGP WKSVTAQNI GFKHISGGL SNLLYHISL PESLVEESK DLGEPQEVL IRVYGQTHG EHALEALIT ESVVFTLLS ERGLGPKLH GIFPGGRIE QYINARPLR TGELADEKL SVKIAQKMA AIHTMEVTF FKSGC
- >TC030588 RC
MSCSLVPLH KEPGWLWDT IDRWLRTCE SKLKSDDVP EFFRNSVDC DFLSEVDWL KKRLEMENC PVVFCHNDM QEGNILIRQ DGPENNNNE DPQIVVIDF EYCSYNYRS FDIANHFVE WVYDYTEAE YPFYKEQLE NYPSKKQRL LFIKAYLEA RGLKENPKK ILREVEVFT LASHFFWGI WGIINAGTS QIPFGYWEY AAARLRNYF QQKKKFTLD MKPLVSSLP TKRKSCSLE
- >TC030588 RA
atggcaaaa aaggtgtcg ggtgactcg aacgaaatg agggaattc gctgcgaga atatgcagg gattatctc tatgggcca tggaaaagc gtcactgca caaaacatt ggcttcaaa catatcagc ggcggcttg tccaacctc ctgtaccac atatccttg ccggaatcc ctcgtagaa gagagcaag gacttgggc gaaccccag gaagtcctg atccgcgtc tacggccaa acccatggc gagcatgcc cttgaggcc ctcatcacc gaaagcgtc gtcttcaca ttactgagc gaaagaggt ctcggccca aaactccac gggattttt ccaggaggc cgtatcgag cagtacatc aatgccaga ccattacgt accggagaa ctcgccgac gagaagttg tcggtaaag attgcacag aaaatggcc gccatacat acaatggag gtgcccctg cacaaggag cccggttgg ctctgggac accatcgat cgatggttg aggacgtgc gaatcgaag ctgaaaagt gatgatgtt cctgaattt ttccgaaac agtgtcgat tgcgacttt ttgtcggaa gtcgattgg ctgaaaaaa cggctcgag atggagaat tgtccggtc gttttttgc cacaacgac atgcaagag ggcaatatt ttgatcaga caagacgga cccgagaat aataataac gaggatccg cagatcgtc gtcatcgat ttcgaatac tgctcatac aactaccgt tcgttcgac atcgccaat catttcgtc gaatgggtg tacgactac accgaagcg gaatacccc ttttataaa gaacaactc gaaaattac ccatcgaag aaacaacgg ctcctattc atcaaagcc tacttagaa gcgcgcggt ttgaaagaa aaccccaag aaaattcta cgcgaagtg gaagtcttc actcttgcc tctcatttc ttttggggg atttgggga attatcaac gcaggcaca tcccaaatc ccgttcggt tactgggaa tatgccgca gcccgtttg agaaattac ttccaacag aaaaagaag ttcacttta gatatgaaa ccgctagtg tcgtcactt ccgactaaa cgaaagagt tgcagtctg gagtga
- >TC030588 RB
atggcaaaa aaggtgtcg ggtgactcg aacgaaatg agggaattc gctgcgaga atatgcagg gattatctc tatgggcca tggaaaagc gtcactgca caaaacatt ggcttcaaa catatcagc ggcggcttg tccaacctc ctgtaccac atatccttg ccggaatcc ctcgtagaa gagagcaag gacttgggc gaaccccag gaagtcctg atccgcgtc tacggccaa acccatggc gagcatgcc cttgaggcc ctcatcacc gaaagcgtc gtcttcaca ttactgagc gaaagaggt ctcggccca aaactccac gggattttt ccaggaggc cgtatcgag cagtacatc aatgccaga ccattacgt accggagaa ctcgccgac gagaagttg tcggtaaag attgcacag aaaatggcc gccatacat acaatggag gtaacattt tttaagtca ggttgttga
- >TC030588 RC
atgtcttgc agtttagtg cccctgcac aaggagccc ggttggctc tgggacacc atcgatcga tggttgagg acgtgcgaa tcgaagctg aaaagtgat gatgttcct gaatttttc cgaaacagt gtcgattgc gactttttg tcggaagtc gattggctg aaaaaacgg ctcgagatg gagaattgt ccggtcgtt ttttgccac aacgacatg caagagggc aatattttg atcagacaa gacggaccc gagaataat aataacgag gatccgcag atcgtcgtc atcgatttc gaatactgc tcatacaac taccgttcg ttcgacatc gccaatcat ttcgtcgaa tgggtgtac gactacacc gaagcggaa taccccttt tataaagaa caactcgaa aattaccca tcgaagaaa caacggctc ctattcatc aaagcctac ttagaagcg cgcggtttg aaagaaaac cccaagaaa attctacgc gaagtggaa gtcttcact cttgcctct catttcttt tgggggatt tggggaatt atcaacgca ggcacatcc caaatcccg ttcggttac tgggaatat gccgcagcc cgtttgaga aattacttc caacagaaa aagaagttc actttagat atgaaaccg ctagtgtcg tcacttccg actaaacga aagagttgc agtctggag tga
- >TC030588 RA
attgccccc tctgtccac ctcagataa aatttttat ctttatagt atacaccag tttctctcg agcactcat cagtctcgc ttcgaccaa agtaggact actttagtg tctcaataa tccacaaat ttcatttca gtgctgtct catttcggt gcaataaag ccctcagcg ttcaaaaag catggcaaa aaaggtgtc gggtgactc gaacgaaat gagggaatt cgctgcgag aatatgcag ggattatct ctatgggcc atggaaaag cgtcactgc acaaaacat tggcttcaa acatatcag cggcggctt gtccaacct cctgtacca catatcctt gccggaatc cctcgtaga agagagcaa ggacttggg cgaacccca ggaagtcct gatccgcgt ctacggcca aacccatgg cgagcatgc ccttgaggc cctcatcac cgaaagcgt cgtcttcac attactgag cgaaagagg tctcggccc aaaactcca cgggatttt tccaggagg ccgtatcga gcagtacat caatgccag accattacg taccggaga actcgccga cgagaagtt gtcggtaaa gattgcaca gaaaatggc cgccataca tacaatgga ggtgcccct gcacaagga gcccggttg gctctggga caccatcga tcgatggtt gaggacgtg cgaatcgaa gctgaaaag tgatgatgt tcctgaatt tttccgaaa cagtgtcga ttgcgactt tttgtcgga agtcgattg gctgaaaaa acggctcga gatggagaa ttgtccggt cgttttttg ccacaacga catgcaaga gggcaatat tttgatcag acaagacgg acccgagaa taataataa cgaggatcc gcagatcgt cgtcatcga tttcgaata ctgctcata caactaccg ttcgttcga catcgccaa tcatttcgt cgaatgggt gtacgacta caccgaagc ggaataccc cttttataa agaacaact cgaaaatta cccatcgaa gaaacaacg gctcctatt catcaaagc ctacttaga agcgcgcgg tttgaaaga aaaccccaa gaaaattct acgcgaagt ggaagtctt cactcttgc ctctcattt cttttgggg gatttgggg aattatcaa cgcaggcac atcccaaat cccgttcgg ttactggga atatgccgc agcccgttt gagaaatta cttccaaca gaaaaagaa gttcacttt agatatgaa accgctagt gtcgtcact tccgactaa acgaaagag ttgcagtct ggagtgaaa tcgtatact tccggttaa ctttcgact agtcgaggt tgctaacta aaggctgat gattttaca cgtgatatt agatatcaa aaagtgtgg caagtgtac ctaagatga agctcgtga ttggttagg tattcgtaa tttatcatc atcacattt tattacgca cgaaatttt attattcgc acagaaatt atttatatt tacaaaatt gaagctaag ccgagggct atctcgttt ggttaagtt tgttggttg ttggaatgt acttaatat tcgagactg agcgctatg ttcgaattt taaatgcat ttaaatgtt acgtttctc aaactatat gtgtatata agttattcg atatgttca attaagatc attctatct tttgtaaat aatttattt taataaaca tgaagtccc aag
- >TC030588 RB
attgccccc tctgtccac ctcagataa aatttttat ctttatagt atacaccag tttctctcg agcactcat cagtctcgc ttcgaccaa atgctgtct catttcggt gcaataaag ccctcagcg ttcaaaaag catggcaaa aaaggtgtc gggtgactc gaacgaaat gagggaatt cgctgcgag aatatgcag ggattatct ctatgggcc atggaaaag cgtcactgc acaaaacat tggcttcaa acatatcag cggcggctt gtccaacct cctgtacca catatcctt gccggaatc cctcgtaga agagagcaa ggacttggg cgaacccca ggaagtcct gatccgcgt ctacggcca aacccatgg cgagcatgc ccttgaggc cctcatcac cgaaagcgt cgtcttcac attactgag cgaaagagg tctcggccc aaaactcca cgggatttt tccaggagg ccgtatcga gcagtacat caatgccag accattacg taccggaga actcgccga cgagaagtt gtcggtaaa gattgcaca gaaaatggc cgccataca tacaatgga ggtaacatt ttttaagtc aggttgttg aattattct gttttattt agaggcggc gttgcaccc atgccatgg gtttgtgtg aacgggtcg tgtgattaa gtaggtctt ggaatttgg attttttcc ttacgactt tgtggaaaa ttacggatg tatttatct cattcggtg tcaatattt acgacaatg catttattt gtagtttct ttgagattt ctttaagga cacgtggtg tatcaccac atttgacat aatttgaat tcctcaagg tcacgatat tgcgtttca gtcggcgct gcacggcga cctttccct gataaatat tcgcaactt atttcgcac tatgataag ggggcgtta cccgataaa accaaaaaa aatgtattt cctgttgtg attcaatta taaattgtg ataaaaaag tagagcatt tgagacaat aaaactgac aaatattta ccaagttac ttgactctg cctcaaggt gattttttc gctcattct agaaattat ttcatattt atagaacta atcaatatg caagagaaa ggctgctaa tgcaaaaca actaactgg aatagtaag ctgtgataa aaaattgac gtctgttat tatcacagc ctgctacag taattgttt caggataag ttatcgtgg tatgctgtt atcgatttt ttcaacgca actgtgtga ctgtttcac atatctggt tatttccgg aattgattt tgtgataaa attgaatat ttaaacact tcaaaaatt gcgtctttg acaaaatcg cggttgctt aacaaattt ttgcaccga gtggcgaaa tcacgtcct acttttgtg cacaatgtt tgcattaaa attaaaaag tgtgacccc ttttgaaaa attatgtgg gacgcctat gtctcaaac gtgaagtta gccattacc aatggcggc tatgttcac accgctgat tttccaaat ttattggaa aaatcctca ttttcgcgt aacacaagc cgaaaacac gaaaaacat gcaaacgga accaaaaat aaacatttc acaatcaat ttcaataga atctgacct ttcatccaa ccggcgaca tcccggact tgattgtca caatttttc acggatgca cttcaattt cagtctaat tttatcaaa cgggacacg caacagttg gaaatgcgt cacttttac gttttccgg cactttttt cgtgaaaat tgcagagaa aatcggcgg actttcggt caagttttc gcggacttt gccaacatt acgtaaaca aaaaccctg tcaaatttt gattttaat taaaaaaac aagttttag tgaactgtt ttaattata aatcgactc atgaccctt gcgggtttg cgtttgtgc ctgaaatta attttgaga aggtggaac atggaaaat taactgcca accgtagca acaaaaaag tggcgctaa atttaaatt tgaatgaat tcggtgaat tacataatg ggttattat tgcgtcttt tgtctgatt ttcaaggaa gtagcagct gataaagct atttactgg ctagcagat caacctggt cgaggttat cacaatttc ttgcactta aaccctgat aaagcaatt gtcattgga tttcgctcg agcgctcac acaaacaag aataaatgt ggttgtttt ag
- >TC030588 RC
tggggattt gttgttgtt atgttctag cgttgtcct atcgtggac ccaaacatc tcgaatcac tctcacgtg ccttcctga ggcgcaaaa caacagccg tttctgtta aaaaacgcc atttgggga cgtccaacc gtaggaatc attcatatg actctaaaa aaataactc aaaattttg accaaaaaa aaatgttcc gagagtgac atgtttttt gtccgagac gaggagtga cgtagaagt acgtctagg ctttgcttg gctaatttt tacccattc atttgcttt cttacaatc attcatcgc ttttggttt gttcgactc actttgaca accacttga ttttagcca atctggtaa gtcccacaa ctgagtcaa acaaaccaa aatatcgta ttatttttg ggaaaacag ttggccgac aggaaacat gtcttgcag tttagtgcc cctgcacaa ggagcccgg ttggctctg ggacaccat cgatcgatg gttgaggac gtgcgaatc gaagctgaa aagtgatga tgttcctga atttttccg aaacagtgt cgattgcga ctttttgtc ggaagtcga ttggctgaa aaaacggct cgagatgga gaattgtcc ggtcgtttt ttgccacaa cgacatgca agagggcaa tattttgat cagacaaga cggacccga gaataataa taacgagga tccgcagat cgtcgtcat cgatttcga atactgctc atacaacta ccgttcgtt cgacatcgc caatcattt cgtcgaatg ggtgtacga ctacaccga agcggaata cccctttta taaagaaca actcgaaaa ttacccatc gaagaaaca acggctcct attcatcaa agcctactt agaagcgcg cggtttgaa agaaaaccc caagaaaat tctacgcga agtggaagt cttcactct tgcctctca tttcttttg ggggatttg gggaattat caacgcagg cacatccca aatcccgtt cggttactg ggaatatgc cgcagcccg tttgagaaa ttacttcca acagaaaaa gaagttcac tttagatat gaaaccgct agtgtcgtc acttccgac taaacgaaa gagttgcag tctggagtg aaatcgtat acttccggt taactttcg actagtcga ggttgctaa ctaaaggct gatgatttt acacgtgat attagatat caaaaagtg tggcaagtg tacctaaga tgaagctcg tgattggtt aggtattcg taatttatc atcatcaca ttttattac gcacgaaat tttattatt cgcacagaa attatttat atttacaaa attgaagct aagccgagg gctatctcg tttggttaa gtttgttgg ttgttggaa tgtacttaa tattcgaga ctgagcgct atgttcgaa ttttaaatg catttaaat gttacgttt ctcaaacta tatgtgtat ataagttat tcgatatgt tcaattaag atcattcta tcttttgta aataattta ttttaataa acatgaagt cccaag
