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Gene information
General information
TC030585 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6669970..6737353
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030585
    iBeetle screen iB_06032

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    mature egg deposition number 2nd lateral oviduct: 2 number 1st lateral oviduct: blocked -
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030585 RA
      MGENTKQVR VDNWGIFFL QRLQMFFSK TDYCDMTLQ 
      FEGNVQLKV HRLVMNACT EYFQFLEQT CALEENVIM 
      MPSDLQADV IVPIVNFMY TGMLEFHLS IFDRLYKTA 
      QLMNITILT KLLDAQKHP VASAVKSLK KKAPDSPLF 
      NAQGKSSVP VLLFVTDIQ KTDPPKSPK PHDPQPDNK 
      PPWKCDRCS TDDESVEFV LYYLYRKHM TDVHNEKFD 
      PKLCKYCGH RCSKHNLLM YHLHTKHGM KPPPNYNFP 
      KCDQCPFIA LNTHLLAKH KLKHDKNEI QCVECKLTF 
      TSQATLTSH IQITGHTGK TSKNNYDCQ YCTKRYQSG 
      VNLFSHVRL QHRNEAIRD GIVSIEEGE DVEDYEEEE 
      NKDEYILPE IITDPTETP KKVNILSNV KVQERHETQ 
      QQLALEPSS EAEALNNVA TGIATSLGL VDIVVINEN 
      QQFFIDDQQ NFNNQTEQP EFILPELSQ NQNEVMTPQ 
      GVLTQSMLS SGDITSTDE LVMVLTDHD YGDNQEEIT 
      NDNSNIVVL YSHPVDGQQ DQFITSQGN IMVNSQTGL 
      IEIRNGATI TSTASQVVM NPPEAQIES IEMIQREIE 
      SHGGELKEE EKPIVDEEK EKEAVVPPN DALIESLTQ 
      GSQLEFTSP LTIEEPEKP VEEPKETEQ ETSDKKEEE 
      TEQPEPADK QNEQLEVNE EEKGGEEEV AKTDDVKEE 
      EEEEEISKT DESKEEEEE ELGKTDDSK GEEEGERDV 
      VPAPTPEKG EEEVEKNET EETAVQLLE ETNTEACPM 
      EIEEEKTTH EENNTVEQE LSQSDTSTS QFNDENSQS 
      SQDSQNKDV EATKISILD DWEDTDSQQ SDNKEKEKV 
      AGETVHKLM DDWEEEEED DKKDSEG
    CDS sequence
    • >TC030585 RA
      atgggggag aacaccaag caagtccgg gtggacaat 
      tggggcatt tttttcctg cagcggctg caaatgttc 
      ttcagcaag acggactac tgcgacatg actctacag 
      ttcgagggg aacgtgcag ctgaaagtg caccggctt 
      gttatgaac gcctgcacg gaatacttc cagtttctg 
      gagcagact tgtgccctt gaggagaac gttatcatg 
      atgccttcg gatctgcaa gccgatgtg attgtgccc 
      attgttaat tttatgtac acggggatg ttggagttc 
      cacttgtcg atcttcgat aggttgtat aaaacggca 
      cagcttatg aatatcacc attttgacc aagttgttg 
      gatgcacag aagcatccg gtggcctcg gcggtcaag 
      agtctcaag aagaaggcg ccggacagc cccctgttc 
      aatgcacaa ggtaagtca tctgttcca gttcttttg 
      tttgtcacc gacattcaa aaaaccgac ccccccaag 
      tcccccaag ccccacgac ccccaaccc gacaacaaa 
      cccccatgg aaatgcgat cgttgcagc accgacgac 
      gaatccgtc gaattcgtc ctctactac ctatacaga 
      aagcacatg accgacgta cataacgaa aaattcgac 
      ccgaaactg tgcaaatac tgcggccat cgatgcagc 
      aaacacaac ctactcatg taccacttg cacaccaaa 
      cacggcatg aaaccgccc cccaactac aacttcccc 
      aagtgcgac caatgtccg ttcatcgcc ctcaacacc 
      cacctactg gccaaacac aaactcaaa cacgacaaa 
      aacgaaata caatgtgtt gagtgcaaa ctgacattc 
      acctcgcag gcgacgcta accagtcac atccaaatc 
      acaggacac acaggcaag actagcaag aacaactac 
      gattgccag tattgtacc aagagatat cagtcggga 
      gtcaatttg ttctcacac gtgcgacta cagcacagg 
      aatgaggcg attcgggac gggattgtg agcattgaa 
      gaaggggaa gatgtggag gattatgaa gaagaagag 
      aacaaagat gagtacatt ttgccggaa attatcacc 
      gatccgacg gaaacaccc aaaaaagtc aatatttta 
      tcgaatgtg aaggtgcaa gaacgacac gagacgcaa 
      caacaactc gccctggag ccgagctcc gaagcagag 
      gccttgaac aacgtcgcc acaggaatt gccaccagc 
      ttaggtctg gtggatatc gtggtcata aacgaaaac 
      cagcagttc ttcatcgac gaccagcag aatttcaac 
      aaccaaacg gaacagccc gagtttatt ctcccagaa 
      ttgtcccaa aatcaaaac gaagttatg accccgcaa 
      ggggtccta acccagtca atgctctcc tcaggtgat 
      ataacttca actgatgag ttagttatg gttctaaca 
      gaccacgat tatggcgat aaccaggaa gagataacc 
      aatgataat tccaacatt gtggttctg tacagccac 
      ccagtcgat gggcaacaa gaccagttc ataacctcc 
      caaggcaac atcatggtt aattcacaa accggtctc 
      attgaaatc agaaatggg gcaacaatt acatcgacg 
      gcgagtcaa gtggtcatg aacccgccc gaagcacag 
      atcgagtca attgaaatg atccagagg gagattgag 
      agccacggt ggcgaactg aaggaggaa gaaaaacca 
      attgttgat gaagaaaag gagaaagaa gctgtagtt 
      ccgccaaat gatgccctc attgaaagt ttaacacaa 
      gggagccaa ctcgagttc acctcacct ttgaccatt 
      gaagagccg gaaaaaccg gttgaagaa ccgaaggag 
      accgaacaa gaaacaagt gacaaaaag gaggaagaa 
      acggaacaa ccagaacct gctgacaaa caaaatgaa 
      cagttggag gtgaatgaa gaagaaaag gggggggaa 
      gaagaggtt gccaaaacg gacgacgtt aaggaggaa 
      gaagaagaa gaagagatt agtaaaact gacgagagt 
      aaggaggag gaggaagaa gaattgggg aaaactgac 
      gacagtaag ggggaagaa gagggagag cgggatgta 
      gtcccagcc cccacgcca gaaaaaggg gaggaagag 
      gttgagaaa aatgagacg gaggaaaca gcagtgcaa 
      ctgttggaa gagacaaac acggaagct tgtcctatg 
      gaaatagaa gaggagaaa acaacgcac gaggaaaat 
      aatacagtg gagcaggag ttgtcacaa agtgacacg 
      tccactagt cagtttaac gacgaaaat tcccagtct 
      tcgcaagat tcgcaaaat aaggatgta gaagcgacg 
      aaaatatcc atcttggac gattgggaa gatactgat 
      tcgcaacag tccgataat aaggaaaag gaaaaggtt 
      gcgggggaa acggtacat aaattgatg gacgattgg 
      gaggaggag gaggaagat gataagaaa gatagtgaa 
      ggatga
    mRNA sequence
    • >TC030585 RA
      ctacgaata caaagaagt agtagcgta tcgccaata 
      tgtttatgc gtctgtaga gatggagct aatattttc 
      aatataagg aaaagggcc ggctcggga ctagtatga 
      ggttatatc cgaagatgg gggagaaca ccaagcaag 
      tccgggtgg acaattggg gcatttttt tcctgcagc 
      ggctgcaaa tgttcttca gcaagacgg actactgcg 
      acatgactc tacagttcg aggggaacg tgcagctga 
      aagtgcacc ggcttgtta tgaacgcct gcacggaat 
      acttccagt ttctggagc agacttgtg cccttgagg 
      agaacgtta tcatgatgc cttcggatc tgcaagccg 
      atgtgattg tgcccattg ttaatttta tgtacacgg 
      ggatgttgg agttccact tgtcgatct tcgataggt 
      tgtataaaa cggcacagc ttatgaata tcaccattt 
      tgaccaagt tgttggatg cacagaagc atccggtgg 
      cctcggcgg tcaagagtc tcaagaaga aggcgccgg 
      acagccccc tgttcaatg cacaaggta agtcatctg 
      ttccagttc ttttgtttg tcaccgaca ttcaaaaaa 
      ccgaccccc ccaagtccc ccaagcccc acgaccccc 
      aacccgaca acaaacccc catggaaat gcgatcgtt 
      gcagcaccg acgacgaat ccgtcgaat tcgtcctct 
      actacctat acagaaagc acatgaccg acgtacata 
      acgaaaaat tcgacccga aactgtgca aatactgcg 
      gccatcgat gcagcaaac acaacctac tcatgtacc 
      acttgcaca ccaaacacg gcatgaaac cgcccccca 
      actacaact tccccaagt gcgaccaat gtccgttca 
      tcgccctca acacccacc tactggcca aacacaaac 
      tcaaacacg acaaaaacg aaatacaat gtgttgagt 
      gcaaactga cattcacct cgcaggcga cgctaacca 
      gtcacatcc aaatcacag gacacacag gcaagacta 
      gcaagaaca actacgatt gccagtatt gtaccaaga 
      gatatcagt cgggagtca atttgttct cacacgtgc 
      gactacagc acaggaatg aggcgattc gggacggga 
      ttgtgagca ttgaagaag gggaagatg tggaggatt 
      atgaagaag aagagaaca aagatgagt acattttgc 
      cggaaatta tcaccgatc cgacggaaa cacccaaaa 
      aagtcaata ttttatcga atgtgaagg tgcaagaac 
      gacacgaga cgcaacaac aactcgccc tggagccga 
      gctccgaag cagaggcct tgaacaacg tcgccacag 
      gaattgcca ccagcttag gtctggtgg atatcgtgg 
      tcataaacg aaaaccagc agttcttca tcgacgacc 
      agcagaatt tcaacaacc aaacggaac agcccgagt 
      ttattctcc cagaattgt cccaaaatc aaaacgaag 
      ttatgaccc cgcaagggg tcctaaccc agtcaatgc 
      tctcctcag gtgatataa cttcaactg atgagttag 
      ttatggttc taacagacc acgattatg gcgataacc 
      aggaagaga taaccaatg ataattcca acattgtgg 
      ttctgtaca gccacccag tcgatgggc aacaagacc 
      agttcataa cctcccaag gcaacatca tggttaatt 
      cacaaaccg gtctcattg aaatcagaa atggggcaa 
      caattacat cgacggcga gtcaagtgg tcatgaacc 
      cgcccgaag cacagatcg agtcaattg aaatgatcc 
      agagggaga ttgagagcc acggtggcg aactgaagg 
      aggaagaaa aaccaattg ttgatgaag aaaaggaga 
      aagaagctg tagttccgc caaatgatg ccctcattg 
      aaagtttaa cacaaggga gccaactcg agttcacct 
      cacctttga ccattgaag agccggaaa aaccggttg 
      aagaaccga aggagaccg aacaagaaa caagtgaca 
      aaaaggagg aagaaacgg aacaaccag aacctgctg 
      acaaacaaa atgaacagt tggaggtga atgaagaag 
      aaaaggggg gggaagaag aggttgcca aaacggacg 
      acgttaagg aggaagaag aagaagaag agattagta 
      aaactgacg agagtaagg aggaggagg aagaagaat 
      tggggaaaa ctgacgaca gtaaggggg aagaagagg 
      gagagcggg atgtagtcc cagccccca cgccagaaa 
      aaggggagg aagaggttg agaaaaatg agacggagg 
      aaacagcag tgcaactgt tggaagaga caaacacgg 
      aagcttgtc ctatggaaa tagaagagg agaaaacaa 
      cgcacgagg aaaataata cagtggagc aggagttgt 
      cacaaagtg acacgtcca ctagtcagt ttaacgacg 
      aaaattccc agtcttcgc aagattcgc aaaataagg 
      atgtagaag cgacgaaaa tatccatct tggacgatt 
      gggaagata ctgattcgc aacagtccg ataataagg 
      aaaaggaaa aggttgcgg gggaaacgg tacataaat 
      tgatggacg attgggagg aggaggagg aagatgata 
      agaaagata gtgaaggat gaatttgta aataaggat 
      tcactatat tattagagg tcgtttatt tgtaggaat 
      tgtcaagcg ttttatcag tttactata ctttatagt 
      tttatttac aataaatat atttaaaag aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY