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Gene information
General information
TC030580 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11552428..11556420
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030580
    iBeetle screen iB_06031

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    mandible not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna sometimes decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs tibiotarsus sometimes bent - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030580 RA
      MDIEEFRTR GKEMVDYIC QYLENIESQ RVHPDVETG 
      YLRKLLPSE APEQPEEWD NIMYDVDKN IMPGITHWQ 
      HPRFHAYFP SGNSFPSIL GDMLSDAIG CIGFSWAAS 
      PACTELETI VMDWFGKAI GLPSDFITS SEGSTGGGV 
      IQTSASECV LVSMLAARN QAIKYLKTE NPQMEDSAF 
      LPKLVGYCS KEAHSCVEK AAKILLVKL RILDPDENG 
      SLRGDKLRE EMEKDKEKG LFPFFVSAI LGSTASCSF 
      DNLEEIGPV CKEQKRCWL HVDAAYAGN GFICPELKY 
      LLNGIDYAD SFNTNPNKW LLVNFDCSC LWVRDRIRL 
      THALVVDPL YLQHANADE SIDYRHWGI PLSRRFRSL 
      KLWFVIRKY GLSGLRAYI RNHIKLAKH FEQLVLQDT 
      RFEVVNDVR LGLVCFRLM ACDAANQKL LAMINASGK 
      IHMTPSVVR NKYIIRFCV DAEHAKEED VDYAWEVIK 
      EFAVETLAT GAKERPPLT RQKSKRFSF TRSISKEMY 
      QRTLSRSNL CDGATPILV ADSDEEDTL VEDTTMLPD 
      EPDEVFIDK QEFVNGFRI NDKEIKYNG VS
    CDS sequence
    • >TC030580 RA
      atggacatc gaagagttt cgaacacgc ggcaaggaa 
      atggtcgat tacatttgc cagtatctg gagaatatc 
      gagtcccag agggttcac ccggacgtg gaaacgggc 
      tacttgagg aagcttttg cccagcgag gcgcccgaa 
      cagcccgaa gaatgggac aatatcatg tacgacgtc 
      gacaagaac atcatgcct gggataacg cactggcag 
      catccccga tttcacgcg tactttccc tcaggaaac 
      tccttcccg tccatcctg ggggacatg ctgtcggac 
      gccatcggc tgcatcggc ttttcatgg gccgctagt 
      cccgcctgc acggagctg gagaccatc gtcatggac 
      tggttcggc aaagccatc ggcctcccc tccgacttc 
      atcacctcc tcggagggc agcaccggc ggcggcgtc 
      atccagacc tccgccagc gagtgcgtc ctagtctcc 
      atgctggca gcccgcaac caggccatc aaatacctc 
      aaaacggaa aacccccaa atggaggac agcgccttc 
      ctcccgaaa ctagtcggc tattgctcc aaagaggcc 
      cactcctgc gtggaaaaa gctgcgaaa atcctccta 
      gtcaagctg cgaatcctg gatcctgac gaaaacggg 
      agcttacgg ggcgataaa ctccgggag gagatggag 
      aaggacaag gagaagggt ttgttcccg tttttcgtt 
      tcggcgatt ttgggctcg actgcctcc tgctccttc 
      gacaatctg gaggagatc gggccggtt tgcaaggaa 
      cagaagcgg tgttggttg cacgtggac gcggcctac 
      gccggaaac gggtttatt tgtcccgaa ttgaagtat 
      ttactcaat gggatcgac tacgctgat agttttaac 
      acgaatccg aacaagtgg cttttggtt aatttcgac 
      tgttcctgc ttgtgggtc agggaccgg attcgacta 
      acgcacgcc ctcgtggtg gaccccctg tacctgcag 
      cacgccaac gctgacgag tccatcgac tacaggcac 
      tgggggatt cccctaagc cggcggttc cgctccttg 
      aaactgtgg ttcgtcatc cggaagtac ggcttaagc 
      ggcctccgg gcctacatc cggaaccac atcaaactg 
      gccaaacac ttcgaacag ttggtcctg caggacacc 
      cggttcgag gtcgtgaat gacgtgagg ctgggcctt 
      gtgtgtttc cggttgatg gcgtgcgat gccgccaac 
      cagaagctg ctagcgatg atcaacgcc tctgggaag 
      attcacatg acaccgtcg gtggtccgg aacaagtac 
      attatccgg ttctgtgtg gacgcggag catgccaaa 
      gaggaggac gttgattat gcgtgggag gtgatcaag 
      gagttcgct gttgaaacg ctggcgacg ggggccaag 
      gagcggccg ccgctcacg agacagaag tcgaagcgg 
      ttctcgttc accaggagc atttcgaag gagatgtac 
      cagaggact ttgagtcgg tcgaatttg tgcgacggg 
      gcgacgccc attcttgtg gcggattcg gatgaggag 
      gacacgctg gttgaggac acgacgatg ctgccagat 
      gagcccgat gaggttttt attgacaaa caagagttt 
      gttaatggg tttaggata aatgacaaa gagattaag 
      tacaatggg gttagttaa
    mRNA sequence
    • >TC030580 RA
      aggaagtac ttttattac gtcgatttt atgggaaat 
      cagccttga aatgggctt ttatgaagt cctgttgga 
      agtccatga aattccgga aaacctccc cgttttctg 
      tctattatt agcctgcag gtagttgtc ttcgtcaat 
      taataaatt ggattatgc cacaaccct atacgtagc 
      cacacagaa catgaatgc gtttgacta gaggttccg 
      acttcagag tggggcaac cttaaatat aaaagtata 
      ccttggcag tatgcagtg attttgtgg tacgctccc 
      agtgctaag ttagtgtga caatggaca tcgaagagt 
      ttcgaacac gcggcaagg aaatggtcg attacattt 
      gccagtatc tggagaata tcgagtccc agagggttc 
      acccggacg tggaaacgg gctacttga ggaagcttt 
      tgcccagcg aggcgcccg aacagcccg aagaatggg 
      acaatatca tgtacgacg tcgacaaga acatcatgc 
      ctgggataa cgcactggc agcatcccc gatttcacg 
      cgtactttc cctcaggaa actccttcc cgtccatcc 
      tgggggaca tgctgtcgg acgccatcg gctgcatcg 
      gcttttcat gggccgcta gtcccgcct gcacggagc 
      tggagacca tcgtcatgg actggttcg gcaaagcca 
      tcggcctcc cctccgact tcatcacct cctcggagg 
      gcagcaccg gcggcggcg tcatccaga cctccgcca 
      gcgagtgcg tcctagtct ccatgctgg cagcccgca 
      accaggcca tcaaatacc tcaaaacgg aaaaccccc 
      aaatggagg acagcgcct tcctcccga aactagtcg 
      gctattgct ccaaagagg cccactcct gcgtggaaa 
      aagctgcga aaatcctcc tagtcaagc tgcgaatcc 
      tggatcctg acgaaaacg ggagcttac ggggcgata 
      aactccggg aggagatgg agaaggaca aggagaagg 
      gtttgttcc cgtttttcg tttcggcga ttttgggct 
      cgactgcct cctgctcct tcgacaatc tggaggaga 
      tcgggccgg tttgcaagg aacagaagc ggtgttggt 
      tgcacgtgg acgcggcct acgccggaa acgggttta 
      tttgtcccg aattgaagt atttactca atgggatcg 
      actacgctg atagtttta acacgaatc cgaacaagt 
      ggcttttgg ttaatttcg actgttcct gcttgtggg 
      tcagggacc ggattcgac taacgcacg ccctcgtgg 
      tggaccccc tgtacctgc agcacgcca acgctgacg 
      agtccatcg actacaggc actggggga ttcccctaa 
      gccggcggt tccgctcct tgaaactgt ggttcgtca 
      tccggaagt acggcttaa gcggcctcc gggcctaca 
      tccggaacc acatcaaac tggccaaac acttcgaac 
      agttggtcc tgcaggaca cccggttcg aggtcgtga 
      atgacgtga ggctgggcc ttgtgtgtt tccggttga 
      tggcgtgcg atgccgcca accagaagc tgctagcga 
      tgatcaacg cctctggga agattcaca tgacaccgt 
      cggtggtcc ggaacaagt acattatcc ggttctgtg 
      tggacgcgg agcatgcca aagaggagg acgttgatt 
      atgcgtggg aggtgatca aggagttcg ctgttgaaa 
      cgctggcga cgggggcca aggagcggc cgccgctca 
      cgagacaga agtcgaagc ggttctcgt tcaccagga 
      gcatttcga aggagatgt accagagga ctttgagtc 
      ggtcgaatt tgtgcgacg gggcgacgc ccattcttg 
      tggcggatt cggatgagg aggacacgc tggttgagg 
      acacgacga tgctgccag atgagcccg atgaggttt 
      ttattgaca aacaagagt ttgttaatg ggtttagga 
      taaatgaca aagagatta agtacaatg gggttagtt 
      aagagtgtg attgggtgt gtagatgtg tagacacaa 
      cagtattat tttaagtaa taatcttaa gttatttgt 
      tcattcaca tatcattag acgtaagga cggttgtgt 
      accaagtga tcatttgaa ttcattaca attagagta 
      cctactgaa actgatatt gaaagaatg gttaagact 
      tgagtctac caactattc ggaggaaac tctcctact 
      ctaattaca aatgacgac atataatac tcgctcatt 
      agtattaag tagcgtcct tgttgttgt taaagagtt 
      tttttactc atttataaa atgtagacg taataaatt 
      atgatatta att
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