Status
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Gene information
General information
TC031640
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030481,TC002053
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5916251..5926706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031640
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06027

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031640 RA
      MGASIHYFN YFYGHSQSS KFPTSSKSL KARIFNYVL 
      YPISCTILA FMLYNFRYM NDNIFKIAR NCQSITSFG 
      QIFTRHFIY AQHSFLLEN LLKNTAKNW KYDPKNPLF 
      GHKTWTRVI LVKSLKIMS FFVVTFIII LPFFCQDID 
      LPQACWVPG SGHKMQLLI YFLQSVCLL EPMILLDMV 
      DSVFLLTGV ELEIQFILL RKAIKNVQI SEQENQNFC 
      MGKLKLYAS YHQFLLDQH TVLKQAFSS FFFLQYLVS 
      VQGLCVEIF VIKQASTIE QIFFSATYI VANAMFLSF 
      AFLAASYLE IEAEALPHA IYSIDWYNG DEKLGKHVL 
      FMLMRAQKP LILTGAGMF AVTRNALLQ TYLDKITTK 
      ECCKGFILL PNKLACVPY CSKKCTNSR CIQPETCLC 
      NPGYLKHPK NKYWCTPKC SKACINGKC TAPETCTCN 
      NNFVKDLKN KYQCNPACT KPCLNGKCI KPETCACNT 
      GYAKDAQSN YLCKPTCSK TCTNGKCTS PETCTCNPG 
      FTRDTKNKY LCNPTCKTA CINGKCSGP ETCTCNPGF 
      IKDLKNKNL CNPVCKSAC INGKCSRPE ICTCNTGFI 
      KDLKNKNQC NPACTKPCV NGKCIKPET CACNTGFVK 
      DGRSNYLCK PTCSKTCTN GKCTSPETC TCNPGFTRD 
      AKNKYLCNP TCKTACSNG KCSGPETCT CNPGFTKNM 
      SNKYLCNPV CSKTCINGK CSGPESCTC NPGFTKDTS 
      NKYLCKPVC TKPCINGKC SGPESCTCN YGYAKDATN 
      KYLCNPVCN QACINGNCS APETCTCNS GYTLDVKNK 
      YGCNPVCSE SCINGKCAA PESCLCNPG YKKDAKNVY 
      WCYPVCSSP CINGQCSAP ETCTCYPGF TPDAQNRHV 
      CNPTCNQTC INGKCSSPE TCSCDLGYT PDVTNKYKC 
      NPACSKPCI NGKCDAPET CTCNPGFTS DPKNRFICN 
      PSCNKTCIN GECSAPETC SCNQGFAVN SDNKYVCSP 
      VCSHACVNG QCSAPETCA CNPGYVTDT LDKYLCNPI 
      CNTTCINGK CSGPEFCTC NPGYVKNDT SVCTPHCNN 
      ECFNGYCDA PETCACNNG YKNVDNVCV SVTSDGLHE 
      GKSLVTLLG SFMFPLIGC SLVLFVIIV ITIVVIVRR 
      RKQRLRARI EYPLYEGQR LVPPRSYHS DDDDDDEVR 
      HALDHDDEE DGNNSTDKG LYVGAPCEI VCNSKLLHV 
      YCNPITGVC ECEKNYPVK LNPYTGCGK PKRLGEQCY 
      YRETCQYTD VHSACVQVH HNAICQCQD GYHSVSIQK 
      PSKRVFCAE DLEVMTTDF STFAGVLSG IAILSGLIC 
      FVLHLFNQN MYGPRRHRF GNANLAPPI LFSSDPGSL 
      PMPIIDRSL TSRTSSHRT LASLPSRRA SSAQGCRGI 
      AVSASRAGA ARNAAILLL SSYQSDPYQ PPLYEYKFG 
      TICTLFPFL RVLPSLTSI HSGNSIKSY SQRRYERER 
      ELKEEREMQ RRLARMSSS ANSSGKIAP TPSPHSTDD 
      LLPTLEEDK QKESSWIAI VPPLAIQEE SSSSFHEEV 
      PSTSKQFP
    CDS sequence
    • >TC031640 RA
      atgggcgcc agcatccac tacttcaac tatttctac 
      ggccacagc cagtcctca aaattccca acctcctca 
      aaatccctc aaagcgcga atcttcaac tacgttttg 
      tacccaatt tcttgcaca attcttgcc ttcatgttg 
      tacaatttt cggtacatg aacgacaat attttcaaa 
      atcgccaga aattgccaa tcgatcaca tcctttggc 
      cagattttt acgcgtcac ttcatctac gcccagcac 
      tcttttttg ttagaaaac ctcctgaaa aacaccgca 
      aaaaactgg aaatatgac ccaaaaaac ccacttttt 
      gggcacaag acctggact cgtgtaatc ctcgtcaaa 
      agtctcaaa atcatgtca tttttcgtg gtcactttt 
      attataatt ttgccgttt ttttgccaa gatatcgat 
      ttgccgcaa gcttgctgg gtgccaggt tctgggcac 
      aaaatgcaa cttttgatc tattttctg caatcggtt 
      tgtttgctc gaaccgatg attttgctc gacatggtc 
      gattcggtg tttcttctg actggagtg gagctcgaa 
      atacagttc attttattg cggaaagcg atcaaaaat 
      gtgcaaata agcgagcaa gaaaaccaa aatttttgt 
      atggggaaa ttgaaattg tacgcatct tatcaccag 
      tttctctta gaccaacac actgtattg aagcaagct 
      ttttcgtcc tttttcttc ctgcaatat ttggtgtcg 
      gtgcaagga ctttgcgtt gaaattttt gtgatcaaa 
      caagcctca acgatcgag caaattttt ttctcagct 
      acttatatt gtagccaat gctatgttt ctctcattt 
      gcattttta gcagcaagt tatctcgaa attgaggct 
      gaggctttg cctcacgca atttattca attgattgg 
      tataatggt gacgagaaa ttagggaaa cacgttctt 
      tttatgtta atgagggcc caaaagccc ttaatattg 
      actggagct ggaatgttt gcagtcacg agaaatgcc 
      ttacttcag acctactta gataaaatc acaactaaa 
      gaatgttgt aaaggcttc atccttcta ccaaataaa 
      ctcgcttgt gttccttat tgttccaag aaatgcact 
      aactctaga tgcattcaa ccagaaacg tgtttgtgc 
      aaccctgga tatttaaaa caccctaaa aataagtac 
      tggtgcacc cctaaatgt tccaaagct tgtatcaat 
      ggaaaatgc acagctccg gaaacttgt acttgtaat 
      aacaatttc gttaaagat ttgaaaaat aagtaccag 
      tgtaaccca gcttgtact aagccttgt ctcaatgga 
      aaatgcatc aaacctgaa acttgtgct tgtaatact 
      ggttatgca aaagacgct caaagcaat tatttgtgt 
      aaaccgact tgtagtaaa acttgtact aacggtaag 
      tgtacaagt ccagagact tgtacttgt aaccccggc 
      tttacacga gatacaaaa aacaaatat ttgtgcaac 
      ccgacttgt aaaactgct tgtattaac ggcaagtgc 
      tcaggacct gagacttgt acttgtaat cccggtttt 
      atcaaagat ttaaaaaac aaaaacttg tgtaatcca 
      gtttgcaaa agtgcttgt attaacggt aagtgctca 
      agacccgaa atttgtact tgtaatact ggtttcatc 
      aaagatttg aaaaataaa aatcagtgt aacccggcg 
      tgtactaaa ccttgtgtc aatggaaaa tgcattaaa 
      cccgaaact tgtgcttgt aatactggt ttcgtgaaa 
      gacggtcga agcaattat ttgtgtaaa ccaacttgt 
      agtaaaact tgtactaac ggtaagtgt acaagtccc 
      gagacttgt acttgtaac cccggcttt acacgagat 
      gcaaaaaac aaatatttg tgtaacccg acttgtaaa 
      actgcttgt agtaacggc aaatgctca ggacctgag 
      acttgtact tgtaatccc ggctttacc aaaaatatg 
      agtaacaaa tatttgtgt aaccccgtc tgtagcaag 
      acttgtatt aacggcaaa tgctcaggt cctgagtcg 
      tgcacttgt aatcccggt ttcaccaaa gacacaagt 
      aacaaatac ttgtgtaaa cccgtttgt accaagcct 
      tgtattaac ggcaagtgc tcaggtcct gagtcatgc 
      acttgtaat tacggttac gctaaagac gctacgaat 
      aaatacttg tgtaacccc gtttgtaac caagcttgt 
      attaacggt aattgctca gctcctgag acgtgcact 
      tgcaactct ggttacacc ctagacgtt aaaaacaaa 
      tatggttgt aatcccgtc tgttccgag tcttgtatt 
      aacggtaaa tgcgcagct cctgagtca tgcctttgt 
      aaccctggt tataaaaaa gatgcaaaa aatgtctat 
      tggtgttat ccagtttgt tctagtcct tgtattaac 
      ggtcaatgc tcagctcct gagacttgt acttgttat 
      cccggcttt acgccagat gctcagaac cgacacgtt 
      tgtaacccg acttgtaac caaacttgt attaacggc 
      aagtgctca tctcctgag acatgttcc tgcgatttg 
      ggttacacc cctgacgtg acaaataaa tacaaatgt 
      aacccggct tgtagtaaa ccttgtatt aacgggaaa 
      tgtgacgct cctgagacg tgcacatgt aacccgggt 
      tttacaagc gatccaaaa aatcgtttt atatgtaac 
      ccgtcttgt aacaaaact tgtattaac ggcgagtgc 
      tcagcacct gagacgtgt tcatgtaac caaggtttt 
      gccgttaat agtgacaat aaatacgtt tgtagcccg 
      gtttgtagt catgcttgt gttaacggt caatgctca 
      gctcctgag acttgtgct tgtaaccct ggttacgta 
      acagacaca cttgataag tacttgtgt aacccgatt 
      tgtaacacg acttgtatt aacggtaaa tgctcaggg 
      cctgagttt tgtacttgt aatcccggt tacgttaaa 
      aatgacaca agtgtttgt accccacat tgtaacaat 
      gaatgtttt aacggttat tgtgacgct cctgagact 
      tgtgcttgt aataacggt tacaaaaat gttgacaat 
      gtttgcgtg tctgtaact agcgatgga ctacacgag 
      gggaaaagc ttagttacg ttacttggg tcgtttatg 
      tttccgtta attgggtgt tcgctcgta ttatttgta 
      ataattgtg attacaatt gttgtaatc gtcaggcga 
      aggaaacaa aggttgagg gcaagaatc gagtatcca 
      ctctacgaa gggcagaga ttggtgccg ccgaggagt 
      tatcattcg gatgatgat gatgatgac gaggttcgg 
      catgctctt gatcatgat gatgaagag gatgggaat 
      aattcaaca gacaaagga ctctatgtg ggagctccg 
      tgcgaaatc gtatgtaac tcgaaacta ctccacgtc 
      tactgcaac cccatcacg ggggtgtgc gagtgcgag 
      aagaactac cccgtcaag ctcaacccg tatacagga 
      tgtggaaaa ccgaaaagg ttgggagag cagtgctac 
      taccgggag acctgtcaa tacaccgat gtccattcg 
      gcctgtgtc caggtccac cataacgcc atttgccaa 
      tgccaagat ggctaccat tccgtttca attcaaaaa 
      ccgtcgaaa agggttttt tgtgccgaa gatctggag 
      gtaatgacg acggatttt tccacattt gccggagtt 
      ttatccgga attgcaatc ctatccgga ttgatttgc 
      ttcgtattg catttattt aaccagaac atgtatgga 
      ccaagacgc catcgtttt ggtaatgct aatttggcg 
      ccccctatt ttattttca agtgaccca ggatcctta 
      ccgatgcct atcatcgat cgcagcttg acgtcgcgg 
      acgtcgagt cataggaca ctagcgtcg ttgccgtcg 
      cgacgggcc agcagtgcc caagggtgc cgcggaata 
      gccgtttcc gcctcccgt gcaggtgcg gcccgcaac 
      gccgccatc ttgctactc tcttcttat caatccgat 
      ccctatcaa ccaccactt tacgagtac aaattcggt 
      acaatctgc acccttttc cctttttta cgtgtctta 
      cccagtttg acttcaatc catagtgga aactccatc 
      aaaagttac agtcaaagg agatacgaa cgggagagg 
      gaactgaag gaggaaagg gagatgcag aggagatta 
      gcacgaatg tcttcatca gctaactcc agcgggaaa 
      atcgcccca actcccagt ccccactca accgacgat 
      cttctcccg acgttagaa gaagacaaa cagaaggaa 
      tcgtcgtgg attgccatt gttcctcct ctcgctata 
      caagaagaa tcgtcatcg agtttccac gaagaagtt 
      ccatcgact tcgaaacag ttcccttga
    mRNA sequence
    • >TC031640 RA
      tactcaaac gcccaaaat gggcgccag catccacta 
      cttcaacta tttctacgg ccacagcca gtcctcaaa 
      attcccaac ctcctcaaa atccctcaa agcgcgaat 
      cttcaacta cgttttgta cccaatttc ttgcacaat 
      tcttgcctt catgttgta caattttcg gtacatgaa 
      cgacaatat tttcaaaat cgccagaaa ttgccaatc 
      gatcacatc ctttggcca gatttttac gcgtcactt 
      catctacgc ccagcactc ttttttgtt agaaaacct 
      cctgaaaaa caccgcaaa aaactggaa atatgaccc 
      aaaaaaccc actttttgg gcacaagac ctggactcg 
      tgtaatcct cgtcaaaag tctcaaaat catgtcatt 
      tttcgtggt cacttttat tataatttt gccgttttt 
      ttgccaaga tatcgattt gccgcaagc ttgctgggt 
      gccaggttc tgggcacaa aatgcaact tttgatcta 
      ttttctgca atcggtttg tttgctcga accgatgat 
      tttgctcga catggtcga ttcggtgtt tcttctgac 
      tggagtgga gctcgaaat acagttcat tttattgcg 
      gaaagcgat caaaaatgt gcaaataag cgagcaaga 
      aaaccaaaa tttttgtat ggggaaatt gaaattgta 
      cgcatctta tcaccagtt tctcttaga ccaacacac 
      tgtattgaa gcaagcttt ttcgtcctt tttcttcct 
      gcaatattt ggtgtcggt gcaaggact ttgcgttga 
      aatttttgt gatcaaaca agcctcaac gatcgagca 
      aattttttt ctcagctac ttatattgt agccaatgc 
      tatgtttct ctcatttgc atttttagc agcaagtta 
      tctcgaaat tgaggctga ggctttgcc tcacgcaat 
      ttattcaat tgattggta taatggtga cgagaaatt 
      agggaaaca cgttctttt tatgttaat gagggccca 
      aaagccctt aatattgac tggagctgg aatgtttgc 
      agtcacgag aaatgcctt acttcagac ctacttaga 
      taaaatcac aactaaaga atgttgtaa aggcttcat 
      ccttctacc aaataaact cgcttgtgt tccttattg 
      ttccaagaa atgcactaa ctctagatg cattcaacc 
      agaaacgtg tttgtgcaa ccctggata tttaaaaca 
      ccctaaaaa taagtactg gtgcacccc taaatgttc 
      caaagcttg tatcaatgg aaaatgcac agctccgga 
      aacttgtac ttgtaataa caatttcgt taaagattt 
      gaaaaataa gtaccagtg taacccagc ttgtactaa 
      gccttgtct caatggaaa atgcatcaa acctgaaac 
      ttgtgcttg taatactgg ttatgcaaa agacgctca 
      aagcaatta tttgtgtaa accgacttg tagtaaaac 
      ttgtactaa cggtaagtg tacaagtcc agagacttg 
      tacttgtaa ccccggctt tacacgaga tacaaaaaa 
      caaatattt gtgcaaccc gacttgtaa aactgcttg 
      tattaacgg caagtgctc aggacctga gacttgtac 
      ttgtaatcc cggttttat caaagattt aaaaaacaa 
      aaacttgtg taatccagt ttgcaaaag tgcttgtat 
      taacggtaa gtgctcaag acccgaaat ttgtacttg 
      taatactgg tttcatcaa agatttgaa aaataaaaa 
      tcagtgtaa cccggcgtg tactaaacc ttgtgtcaa 
      tggaaaatg cattaaacc cgaaacttg tgcttgtaa 
      tactggttt cgtgaaaga cggtcgaag caattattt 
      gtgtaaacc aacttgtag taaaacttg tactaacgg 
      taagtgtac aagtcccga gacttgtac ttgtaaccc 
      cggctttac acgagatgc aaaaaacaa atatttgtg 
      taacccgac ttgtaaaac tgcttgtag taacggcaa 
      atgctcagg acctgagac ttgtacttg taatcccgg 
      ctttaccaa aaatatgag taacaaata tttgtgtaa 
      ccccgtctg tagcaagac ttgtattaa cggcaaatg 
      ctcaggtcc tgagtcgtg cacttgtaa tcccggttt 
      caccaaaga cacaagtaa caaatactt gtgtaaacc 
      cgtttgtac caagccttg tattaacgg caagtgctc 
      aggtcctga gtcatgcac ttgtaatta cggttacgc 
      taaagacgc tacgaataa atacttgtg taaccccgt 
      ttgtaacca agcttgtat taacggtaa ttgctcagc 
      tcctgagac gtgcacttg caactctgg ttacaccct 
      agacgttaa aaacaaata tggttgtaa tcccgtctg 
      ttccgagtc ttgtattaa cggtaaatg cgcagctcc 
      tgagtcatg cctttgtaa ccctggtta taaaaaaga 
      tgcaaaaaa tgtctattg gtgttatcc agtttgttc 
      tagtccttg tattaacgg tcaatgctc agctcctga 
      gacttgtac ttgttatcc cggctttac gccagatgc 
      tcagaaccg acacgtttg taacccgac ttgtaacca 
      aacttgtat taacggcaa gtgctcatc tcctgagac 
      atgttcctg cgatttggg ttacacccc tgacgtgac 
      aaataaata caaatgtaa cccggcttg tagtaaacc 
      ttgtattaa cgggaaatg tgacgctcc tgagacgtg 
      cacatgtaa cccgggttt tacaagcga tccaaaaaa 
      tcgttttat atgtaaccc gtcttgtaa caaaacttg 
      tattaacgg cgagtgctc agcacctga gacgtgttc 
      atgtaacca aggttttgc cgttaatag tgacaataa 
      atacgtttg tagcccggt ttgtagtca tgcttgtgt 
      taacggtca atgctcagc tcctgagac ttgtgcttg 
      taaccctgg ttacgtaac agacacact tgataagta 
      cttgtgtaa cccgatttg taacacgac ttgtattaa 
      cggtaaatg ctcagggcc tgagttttg tacttgtaa 
      tcccggtta cgttaaaaa tgacacaag tgtttgtac 
      cccacattg taacaatga atgttttaa cggttattg 
      tgacgctcc tgagacttg tgcttgtaa taacggtta 
      caaaaatgt tgacaatgt ttgcgtgtc tgtaactag 
      cgatggact acacgaggg gaaaagctt agttacgtt 
      acttgggtc gtttatgtt tccgttaat tgggtgttc 
      gctcgtatt atttgtaat aattgtgat tacaattgt 
      tgtaatcgt caggcgaag gaaacaaag gttgagggc 
      aagaatcga gtatccact ctacgaagg gcagagatt 
      ggtgccgcc gaggagtta tcattcgga tgatgatga 
      tgatgacga ggttcggca tgctcttga tcatgatga 
      tgaagagga tgggaataa ttcaacaga caaaggact 
      ctatgtggg agctccgtg cgaaatcgt atgtaactc 
      gaaactact ccacgtcta ctgcaaccc catcacggg 
      ggtgtgcga gtgcgagaa gaactaccc cgtcaagct 
      caacccgta tacaggatg tggaaaacc gaaaaggtt 
      gggagagca gtgctacta ccgggagac ctgtcaata 
      caccgatgt ccattcggc ctgtgtcca ggtccacca 
      taacgccat ttgccaatg ccaagatgg ctaccattc 
      cgtttcaat tcaaaaacc gtcgaaaag ggttttttg 
      tgccgaaga tctggaggt aatgacgac ggatttttc 
      cacatttgc cggagtttt atccggaat tgcaatcct 
      atccggatt gatttgctt cgtattgca tttatttaa 
      ccagaacat gtatggacc aagacgcca tcgttttgg 
      taatgctaa tttggcgcc ccctatttt attttcaag 
      tgacccagg atccttacc gatgcctat catcgatcg 
      cagcttgac gtcgcggac gtcgagtca taggacact 
      agcgtcgtt gccgtcgcg acgggccag cagtgccca 
      agggtgccg cggaatagc cgtttccgc ctcccgtgc 
      aggtgcggc ccgcaacgc cgccatctt gctactctc 
      ttcttatca atccgatcc ctatcaacc accacttta 
      cgagtacaa attcggtac aatctgcac ccttttccc 
      ttttttacg tgtcttacc cagtttgac ttcaatcca 
      tagtggaaa ctccatcaa aagttacag tcaaaggag 
      atacgaacg ggagaggga actgaagga ggaaaggga 
      gatgcagag gagattagc acgaatgtc ttcatcagc 
      taactccag cgggaaaat cgccccaac tcccagtcc 
      ccactcaac cgacgatct tctcccgac gttagaaga 
      agacaaaca gaaggaatc gtcgtggat tgccattgt 
      tcctcctct cgctataca agaagaatc gtcatcgag 
      tttccacga agaagttcc atcgacttc gaaacagtt 
      cccttgaaa aactcagtg aagtaccaa aactccaag 
      tcgccaggg cctattttg cattttttt gcgttgtgg 
      ttatgtgta atgtgctac aagtataca acgtggcgg 
      actatgtgc attatatgt tacgaatta tcaattttt 
      aaagcgtta aaatagact cataggata ataaacgtt 
      gttgtaaaa a
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