Status
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Gene information
General information
TC030464 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2139502..2143042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030464
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06025

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 1

    vitellogenic egg chamber late size decreased - Number of animals showing the phenotype: 2

    vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 2

    tropharium structure irregular - Number of animals showing the phenotype: 4

    egg chamber complete not present - Number of animals showing the phenotype: 1

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030464 RA
      MGRVFVVGV GMTKFEKPG KRNDDYPGF AKEAILKAL 
      DDCGVKISE VEAAACGYV YGDSTSGQR AVYEVGMTG 
      IPIFNVNNN CSTGSTALL LAKEFVESG KHDCVLAVG 
      FEKMERGSL SAKFLDRTN PLDKHVELM AELTEMNEA 
      PLTAQMFGN AGIEHMKKF GTKPEHFAK IAYKNHKHS 
      VNNPYSQFR EEYSLDQIK NSPAVFGPL TKLQCCPTS 
      DGGAAAVVV SERFVRSHG LEGQAVEIL GMEMATDLP 
      STFTDNSCM KLIGYDMTK TAADRLFRK TNTNPTDVQ 
      VVELHDCFS ANELITYEA LGLCPPGKA GELIDRGDN 
      TYGGKYVVN PSGGLISKG HPLGATGLA QCAELCWQL 
      RGEAGARQV PNVKLALQH NIGLGGAVV VGLYRLGFP 
      EHKNRRVGV RKVAGGGKE SDFLIAPYL RLLEQAMSE 
      DKDSLTERH KGTYGFRVK KSNGEEGFW IVNVKVGKG 
      SIEVNSKLK PDVTFIVND NDIFDLLTG KLNPQKAFF 
      QGKVKIQGN MGLALKLAE LQRTAASHI ADIRAKL
    CDS sequence
    • >TC030464 RA
      atgggtcgc gtttttgtt gtaggagtt ggaatgacc 
      aaatttgaa aaacccgga aaacgcaac gacgattat 
      ccgggcttt gccaaggaa gctatcctg aaggccctc 
      gatgactgc ggggtcaaa atctcggag gtggaagca 
      gccgcctgt ggctacgtc tatggcgac tcaaccagc 
      ggccaacgg gcggtttac gaggtggga atgaccgga 
      atcccgatt ttcaatgtg aacaataat tgctcaacg 
      gggtcaact gccctgctt ttggccaaa gagttcgta 
      gaatcgggg aagcatgac tgtgtcttg gctgtgggt 
      ttcgagaaa atggagcgg gggagtttg tcggcgaaa 
      tttttagac cgcacaaac ccgctggat aagcacgta 
      gaattgatg gccgagttg accgagatg aacgaggct 
      cctttgacc gcccaaatg tttgggaac gcgggcatc 
      gaacatatg aagaaattc gggaccaag ccagagcat 
      ttcgccaaa atcgcatac aagaaccac aaacattcc 
      gtcaataac ccttactcg cagttccgg gaggagtat 
      tccctcgat cagatcaaa aattctcct gctgttttt 
      ggtccttta accaagctg cagtgttgc cctacaagc 
      gacggcggt gctgctgcg gtggtggtc tcagagcgt 
      ttcgtgcga agtcatggc ttggaggga caagctgtt 
      gagattctg ggcatggag atggccacc gatttgccc 
      tcaactttt accgacaac agctgcatg aaattaatc 
      gggtatgac atgacaaaa accgcagcc gatcgccta 
      ttccgcaaa acaaacaca aaccccacc gatgtccaa 
      gtggtcgaa ctccatgac tgtttctca gccaacgaa 
      ttaatcact tacgaagcg ctcggtctg tgccctcct 
      ggcaaagcc ggcgagctg atcgaccgc ggtgataac 
      acttacggt ggcaaatat gttgtcaat cctagcgga 
      ggtttgatt tccaagggc caccctctg ggggccacc 
      ggtttggca caatgtgcc gaattgtgt tggcagttg 
      agaggcgaa gctggcgca cgacaagtt cctaatgtt 
      aaattggcc ttacagcac aatattgga ttagggggt 
      gctgttgtt gtcggtttg tatcgcttg ggattcccc 
      gagcataaa aatcgcaga gttggcgtg cggaaggtg 
      gcaggaggc gggaaagaa agcgatttt ctcattgcg 
      ccgtatttg agattattg gaacaggct atgtccgaa 
      gataaggac agtttgact gaaagacat aagggtact 
      tacgggttc agggttaag aagtccaat ggagaggag 
      ggattttgg attgtcaat gtcaaggtc ggcaagggt 
      agcattgaa gttaattcg aagctgaaa ccagacgtt 
      acgtttatt gtgaacgac aatgacatc tttgacttg 
      ctaacaggg aagttaaac ccgcagaag gcatttttc 
      caaggcaag gtcaagatc caaggcaac atgggtttg 
      gccctaaag ctggctgag ttgcaacgc actgctgcc 
      agtcacatt gcagacatt cgtgcaaaa ttgtaa
    mRNA sequence
    • >TC030464 RA
      gttgacttt caattattt ttatcaaat caacaaacg 
      caagttgtg aaaaataat aatttgcgt cagtggacc 
      aacgtacag ttcccctta ttgtgtgaa attagtcca 
      gaaacaggg aaaatgggt cgcgttttt gttgtagga 
      gttggaatg accaaattt gaaaaaccc ggaaaacgc 
      aacgacgat tatccgggc tttgccaag gaagctatc 
      ctgaaggcc ctcgatgac tgcggggtc aaaatctcg 
      gaggtggaa gcagccgcc tgtggctac gtctatggc 
      gactcaacc agcggccaa cgggcggtt tacgaggtg 
      ggaatgacc ggaatcccg attttcaat gtgaacaat 
      aattgctca acggggtca actgccctg cttttggcc 
      aaagagttc gtagaatcg gggaagcat gactgtgtc 
      ttggctgtg ggtttcgag aaaatggag cgggggagt 
      ttgtcggcg aaattttta gaccgcaca aacccgctg 
      gataagcac gtagaattg atggccgag ttgaccgag 
      atgaacgag gctcctttg accgcccaa atgtttggg 
      aacgcgggc atcgaacat atgaagaaa ttcgggacc 
      aagccagag catttcgcc aaaatcgca tacaagaac 
      cacaaacat tccgtcaat aacccttac tcgcagttc 
      cgggaggag tattccctc gatcagatc aaaaattct 
      cctgctgtt tttggtcct ttaaccaag ctgcagtgt 
      tgccctaca agcgacggc ggtgctgct gcggtggtg 
      gtctcagag cgtttcgtg cgaagtcat ggcttggag 
      ggacaagct gttgagatt ctgggcatg gagatggcc 
      accgatttg ccctcaact tttaccgac aacagctgc 
      atgaaatta atcgggtat gacatgaca aaaaccgca 
      gccgatcgc ctattccgc aaaacaaac acaaacccc 
      accgatgtc caagtggtc gaactccat gactgtttc 
      tcagccaac gaattaatc acttacgaa gcgctcggt 
      ctgtgccct cctggcaaa gccggcgag ctgatcgac 
      cgcggtgat aacacttac ggtggcaaa tatgttgtc 
      aatcctagc ggaggtttg atttccaag ggccaccct 
      ctgggggcc accggtttg gcacaatgt gccgaattg 
      tgttggcag ttgagaggc gaagctggc gcacgacaa 
      gttcctaat gttaaattg gccttacag cacaatatt 
      ggattaggg ggtgctgtt gttgtcggt ttgtatcgc 
      ttgggattc cccgagcat aaaaatcgc agagttggc 
      gtgcggaag gtggcagga ggcgggaaa gaaagcgat 
      tttctcatt gcgccgtat ttgagatta ttggaacag 
      gctatgtcc gaagataag gacagtttg actgaaaga 
      cataagggt acttacggg ttcagggtt aagaagtcc 
      aatggagag gagggattt tggattgtc aatgtcaag 
      gtcggcaag ggtagcatt gaagttaat tcgaagctg 
      aaaccagac gttacgttt attgtgaac gacaatgac 
      atctttgac ttgctaaca gggaagtta aacccgcag 
      aaggcattt ttccaaggc aaggtcaag atccaaggc 
      aacatgggt ttggcccta aagctggct gagttgcaa 
      cgcactgct gccagtcac attgcagac attcgtgca 
      aaattgtaa ttttttact atttttata ttctatggt 
      ttttgttat cagttatat tttggaata attatggta 
      acggactga tggttcggg tgttgacca atgtatact 
      tttttattg aataaacag tggttacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY