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Gene information
General information
TC030104 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15310647..15320426
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030104
    iBeetle screen iB_06020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030104 RA
      MPKTHRSIP KSALNSNAA GWGCLPSSS AFSAGMKSG 
      CGSAQSMGK RLDTHLGLV RALSRRWPT KNTDTTHAI 
      QFSSALSWP QADASHPRP GCVTSRLSR TLLLLLLGS 
      AQRRRQRRV WTTPRRPKA AGEELGVVS TINSSTMYH 
      QDQAVSILG EAIPKRRSV FLESGVNSA DSFKASKVG 
      PAPPIKFIN KSSTDKFGN GAIYEVLKP IYALMRIVG 
      IFPIKNTEP GMFRVAPEL LGYSVVVFV VVMGYIGFI 
      EWDKVEIVR SQEGRFEEA VIDYLFTVY LLPIIINPL 
      VLYEARKLA NVVTDWVNF ERIYYKLTK KKLSVFFGN 
      KPVILTVVL PLLACGVMV VTHITMAHF KIIQVVPYC 
      YINCLIYLI GGFWFMQCD VVGKVASQL AEDFQMALK 
      HVGPSSQVA DYRSLWMLL SKLIRDVGN ASGYTVTFL 
      CLYLFLIIT LTIYGLLSQ LQAGFSTKD IGLTINAGL 
      AIFILYFIC DEAHYASNC LRVQFQKKL LLVELSWMN 
      DEAQQEINM FLKATEMSP TDISLVGFF DVNRNLFKS 
      LLATMVTYL VVLLQFQIS IPEEASPTN STTITTQTP 
      N
    CDS sequence
    • >TC030104 RA
      atgccgaag acccaccga tcaataccc aagtctgct 
      ctcaacagc aacgcagct ggctggggc tgtctccct 
      tcttcttca gctttctca gctgggatg aaaagcggc 
      tgcgggtcc gcccagtcg atggggaag aggttggac 
      actcacctg gggctggtg agggcgctg tccagacgc 
      tggccgacg aagaacaca gacactaca cacgccatc 
      cagttctcg agtgcgctc agttggcct caagctgat 
      gcctcccac ccgcggcct ggttgcgtc acgtcgcgc 
      ttatccagg acgctgctg ctgctgctg ctgggttct 
      gcccaaaga agacgccag aggcgggtg tggacgaca 
      cccagacgc ccaaaagcc gcaggagaa gagctggga 
      gtggtatcg accatcaat tcatccacg atgtaccac 
      caagaccaa gccgtaagc atccttggc gaagccatc 
      ccgaaacga aggagtgtt ttcctggaa agtggtgta 
      aacagtgcc gattcgttc aaagcctcc aaagtcggg 
      cctgcaccc ccgatcaaa ttcatcaac aaatcgtca 
      acagacaaa ttcggaaat ggagcgatt tacgaagtc 
      ctcaaaccg atctatgcc ctaatgagg attgtggga 
      attttcccg attaaaaac accgaacct gggatgttc 
      cgagtggcg ccggagtta ttggggtac tcggtggtg 
      gtgtttgtg gtggttatg gggtacatt gggtttatt 
      gagtgggac aaagttgaa atagtgcgc tcgcaagaa 
      ggccggttt gaagaagca gtgattgat tatttgttc 
      actgtgtat cttttaccc attattatc aatccattg 
      gtgttgtat gaggccagg aagttggca aatgtggtc 
      acggattgg gtcaacttt gagaggatt tactacaag 
      ttgactaag aagaagctt tcggtgttt tttgggaac 
      aagccagtg attctgacc gtggtactg ccgcttttg 
      gcttgtggg gttatggta gtaacccat atcacgatg 
      gcacatttt aaaattatt caggtggtc ccttattgc 
      tacatcaac tgcttgatc tacctaatc gggggcttt 
      tggttcatg caatgcgac gtcgtgggc aaagtggcc 
      tctcaacta gctgaagac ttccaaatg gcgctaaag 
      cacgtgggg ccgtcgtct caagtcgcc gattatcgt 
      tcgctgtgg atgctttta agcaaattg ataagagac 
      gtagggaac gcctccgga tacactgtc actttcctc 
      tgcctctat ctattttta ataataact ctgacaatc 
      tacgggtta ctgtcacag cttcaagcc ggttttagt 
      accaaagat atcggcttg acgataaat gcgggctta 
      gcaattttc attttgtat ttcatttgt gacgaggct 
      cattacgcc tccaactgt ctgcgcgtc cagtttcag 
      aaaaaacta cttttggtc gagttgtcc tggatgaac 
      gatgaggcc caacaggaa atcaatatg ttcctaaag 
      gccacggag atgagtcct acggatata agcctagtt 
      ggctttttc gacgttaat aggaattta tttaagtcg 
      ctgttggca acaatggtg acttatctg gtggtgctc 
      ttgcagttc caaatcagc atacccgag gaagccagt 
      cctaccaac tccaccaca atcactacg caaacaccc 
      aactaa
    mRNA sequence
    • >TC030104 RA
      agaacgcct cccccctgt cgcctccac ctcctgggg 
      atgacgcag gtggcggag ctgggcctg caagggcag 
      caacatgcc gaagaccca ccgatcaat acccaagtc 
      tgctctcaa cagcaacgc agctggctg gggctgtct 
      cccttcttc ttcagcttt ctcagctgg gatgaaaag 
      cggctgcgg gtccgccca gtcgatggg gaagaggtt 
      ggacactca cctggggct ggtgagggc gctgtccag 
      acgctggcc gacgaagaa cacagacac tacacacgc 
      catccagtt ctcgagtgc gctcagttg gcctcaagc 
      tgatgcctc ccacccgcg gcctggttg cgtcacgtc 
      gcgcttatc caggacgct gctgctgct gctgctggg 
      ttctgccca aagaagacg ccagaggcg ggtgtggac 
      gacacccag acgcccaaa agccgcagg agaagagct 
      gggagtggt atcgaccat caattcatc cacgatgta 
      ccaccaaga ccaagccgt aagcatcct tggcgaagc 
      catcccgaa acgaaggag tgttttcct ggaaagtgg 
      tgtaaacag tgccgattc gttcaaagc ctccaaagt 
      cgggcctgc acccccgat caaattcat caacaaatc 
      gtcaacaga caaattcgg aaatggagc gatttacga 
      agtcctcaa accgatcta tgccctaat gaggattgt 
      gggaatttt cccgattaa aaacaccga acctgggat 
      gttccgagt ggcgccgga gttattggg gtactcggt 
      ggtggtgtt tgtggtggt tatggggta cattgggtt 
      tattgagtg ggacaaagt tgaaatagt gcgctcgca 
      agaaggccg gtttgaaga agcagtgat tgattattt 
      gttcactgt gtatctttt acccattat tatcaatcc 
      attggtgtt gtatgaggc caggaagtt ggcaaatgt 
      ggtcacgga ttgggtcaa ctttgagag gatttacta 
      caagttgac taagaagaa gctttcggt gttttttgg 
      gaacaagcc agtgattct gaccgtggt actgccgct 
      tttggcttg tggggttat ggtagtaac ccatatcac 
      gatggcaca ttttaaaat tattcaggt ggtccctta 
      ttgctacat caactgctt gatctacct aatcggggg 
      cttttggtt catgcaatg cgacgtcgt gggcaaagt 
      ggcctctca actagctga agacttcca aatggcgct 
      aaagcacgt ggggccgtc gtctcaagt cgccgatta 
      tcgttcgct gtggatgct tttaagcaa attgataag 
      agacgtagg gaacgcctc cggatacac tgtcacttt 
      cctctgcct ctatctatt tttaataat aactctgac 
      aatctacgg gttactgtc acagcttca agccggttt 
      tagtaccaa agatatcgg cttgacgat aaatgcggg 
      cttagcaat tttcatttt gtatttcat ttgtgacga 
      ggctcatta cgcctccaa ctgtctgcg cgtccagtt 
      tcagaaaaa actactttt ggtcgagtt gtcctggat 
      gaacgatga ggcccaaca ggaaatcaa tatgttcct 
      aaaggccac ggagatgag tcctacgga tataagcct 
      agttggctt tttcgacgt taataggaa tttatttaa 
      gtcgctgtt ggcaacaat ggtgactta tctggtggt 
      gctcttgca gttccaaat cagcatacc cgaggaagc 
      cagtcctac caactccac cacaatcac tacgcaaac 
      acccaacta atccactct atttttaca caagtgttt 
      tatcatatt cataaaacg atcaatgtt atttttagt 
      gctactatt tatttccga gggttggtg tgtttgttc 
      tggggtgca atccgaagt ttagcaacg agggtggca 
      tttgcgtca taagaatag gacatatcg agttttttt 
      aaaaaatta aattatttg taactgatc aattgcgaa 
      ataaatttt ttatcccaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY