Status
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Gene information
General information
TC031984
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030066,TC030068
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3245803..3252257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031984
    iBeetle screen iB_06017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031984 RA
      MLCVTFVLE LVCLFTSVK YTIASFTIL EYSNSTLHR 
      HRRQNGCVL PQHPTNGQW SIYKSNARF SPGESIPSG 
      SILQVKCDQ TYKLDGDAT IVCINEKWT SEVGVCLKC 
      GQKSTRNNP TLIVNGTNA IRGDYPWQV ALYNKTSKV 
      LLCGGSLLN QRVILTAAH CITDDTGKL LNKETYTVA 
      VGKYYRKYD HSEDVNKAQ FSEIHEMFT TRKYKGHIQ 
      NFFGDIAII VTKKIFFLS DRVQPVCID WGFKYQRKL 
      LNPNVSKYG YVSGWGYTA EYKNPSEVL KELKVPIIT 
      NDQCDTDLP EDYQKFMTD DKICAGFLN ARTSVCSGD 
      SGGALVTEY KGRYYAVGI VSLSPQAPT EEGGCDSQQ 
      YTLFTSVYY YINELIFEK EARYRPSHS EIASCEEDS 
      KCQPEPVTT TTTTTTTPK PVTEPTTPN CVLPEHPAS 
      GQWSIYGVQ TKFTPGQFV SPATVLEVT CQKDYKLNG 
      NNLLICHNG KWSPEIGKC SKTCPPVQS TPSTVITCT 
      YKNNETDCS SPPDGTVAK YTCAPFYES TETTKNPVR 
      VCKNGSWDL SKPECVPIC GKKYVRAQS LIVNGRKAK 
      GGEHPWQVA IYRNNKFIC GGALISENL ILTAAHCVR 
      YENREANVD EFTVGVGKN SIRLDDTSD VHAQFSGLQ 
      DIIVPKQFN GLYTNLLAD IALLVSKKN FTISFSVQP 
      VCLDWANSI NLTHNSKGY ISGWGYTAE GTKPADDLQ 
      ELEVSIVSN QDCSQLLTE ELKPFLTFD KICAGDPTH 
      NASACNGDA GGPLVVKHN GRFYINGIV SSAEADHTS 
      GRCRSDQYG LFIKVSEYQ SFILETMAK YNPRSSTPE 
      ITSPKPPPP PPEKPENYC ILPNYPDFG RWALFASGS 
      HSPGMSVVA GTILQVTCQ NRYKLDGDS IILCDKGQW 
      SSEIGKCLR TCPSILTTN KLQATCVFK GKESENCTE 
      PIEGTQVKY QCAPYYEDK NLAQYPVGN CKDGTWDRA 
      TPNCVPICG VKSVQPQQL IIGGNVVKK GDYPWVVAI 
      YHGPQKDFI CSGTLISEK IILTAALCV TNSGGKVRD 
      KSDFAVAVG KYYQEFTDS REKISNVQF SDLEAIHVP 
      DYYRGLDQN YYGDIAVLV AKKSFTFTQ TVQPVCVDF 
      AANYDNKNL TGFVTGWGF TKENGGVSD ELKELKVSL 
      VSVADCRKE LPSEFQKYL LTDKICAGY RNKGLSVCK 
      GDSGGGLVV KSSTTGRYN LAGIVTVAP NSPSGGCNS 
      QQYALYTTI SHHYEKFLS GKINNR
    CDS sequence
    • >TC031984 RA
      atgttgtgt gtaacgttt gtgcttgag ctagtttgt 
      ttatttact tccgttaag tacacaata gccagtttc 
      acaattttg gaatactcc aattccacc ttgcacaga 
      catcggaga caaaatggt tgcgttttg ccacaacat 
      ccgacaaac ggccaatgg tcaatttac aaatcaaac 
      gcccgattt tcacctgga gaatccata ccttcgggc 
      agtattttg caagttaag tgcgaccaa acctacaaa 
      ctagacgga gacgctact attgtctgc atcaacgaa 
      aaatggaca tccgaagta ggcgtttgc ctgaaatgt 
      ggccaaaag tcgaccaga aataacccg acactgatc 
      gtaaatggg actaatgca attcgggga gactaccct 
      tggcaagta gctttgtac aacaagacg tccaaagtg 
      ctactttgt ggaggctct ttactgaat caaagagtg 
      atattaacc gctgctcat tgcataacg gacgacact 
      ggtaaatta ctgaataaa gaaacgtac acagtggca 
      gtaggcaaa tactaccgc aaatatgac cactcagag 
      gacgtaaat aaagcgcaa ttttcggaa atccatgaa 
      atgttcaca acgaggaag tacaaagga cacatacag 
      aattttttc ggcgacatt gctatcata gttacgaaa 
      aaaatattt ttcctgtca gatcgagtc caaccggtt 
      tgcatagac tgggggttc aagtatcaa agaaagctt 
      ctgaacccc aacgtgtct aaatatggc tacgtgtct 
      ggatggggc tacaccgcc gaatacaaa aacccctcc 
      gaggtactc aaagaactg aaagtccca ataatcaca 
      aacgaccag tgtgacacc gatttaccg gaagattac 
      caaaaattc atgactgat gataagatt tgtgcggga 
      tttttaaat gctcgcacg tctgtttgt agcggtgac 
      agtggtggt gctttagta acagaatat aaaggtagg 
      tactacgct gttggaatt gtgagtcta tcgccacaa 
      gcaccaacc gaagaaggt ggttgtgat agtcaacaa 
      tacaccctc ttcactagt gtttactac tacatcaac 
      gaactgatt tttgaaaaa gaagcccga tacagacca 
      tcacattcg gaaattgcg agttgcgaa gaagactcc 
      aaatgtcaa cctgaaccc gtgaccaca actacgacc 
      acgacaacg acaccaaaa ccagtcaca gaaccaaca 
      actccaaat tgcgttttg cccgagcat ccggcctca 
      ggccaatgg tcgatctac ggtgtccaa actaaattt 
      accccagga cagttcgta tcacccgca acagtgctg 
      gaagtaact tgccagaaa gactacaaa ttgaacgga 
      aacaacctc ctgatttgc cacaacggc aagtggtca 
      cccgaaatc ggaaaatgt tcaaagact tgccccccg 
      gtgcaaagc accccttca actgtgata acttgcact 
      tacaaaaac aacgaaact gactgcagc agtcctccg 
      gatggtacc gtcgctaaa tacacctgt gcccccttt 
      tatgagagt accgaaacg acgaaaaat ccggtcagg 
      gtgtgcaaa aacgggtcg tgggatttg tccaagcct 
      gaatgcgtt ccgatttgt gggaagaaa tatgtgcga 
      gcacaatct ttgatagtg aacgggagg aaagcaaaa 
      ggcggcgaa catccctgg caagtggct atttaccga 
      aataacaaa tttatttgt ggtggagct ttaattagc 
      gaaaatctt atactcact gccgcacat tgcgtacgt 
      tatgagaac cgtgaagca aacgttgat gagtttact 
      gttggcgtt ggcaagaat tcgattcgt ttagacgac 
      acatctgat gtccacgca cagttttct gggttgcag 
      gacatcatc gtcccgaaa caattcaat gggctttac 
      actaattta ctggcagat atagcactt ctagttagt 
      aaaaagaac ttcacaatt tcgttttct gtacaaccg 
      gtgtgcctg gattgggct aattcgatt aatttgaca 
      cacaattcc aagggttac atctcaggt tggggctac 
      acggctgaa ggaacaaaa ccggctgat gatttacaa 
      gaactggaa gtgtcaatt gtgtcgaat caagactgt 
      tcccaactt ttaacggaa gaattaaaa ccgttcttg 
      actttcgat aaaatatgt gcaggggac ccaactcac 
      aatgccagt gcgtgcaat ggagacgcc ggtggtcct 
      ctcgttgtg aaacacaac ggtaggttt tacattaat 
      ggaattgtg agtagtgct gaagctgat cacaccagt 
      ggccggtgc agaagtgat caatacgga cttttcatc 
      aaagtttcg gaataccaa tcgttcatt ttggaaact 
      atggccaaa tacaatcca cgctcaagc acccccgaa 
      ataacatca cccaaacct ccaccacca cccccggaa 
      aagcctgaa aattactgc attctacca aattacccc 
      gactttggc agatgggca ctgttcgcg tccggcagt 
      cattccccc ggaatgtca gtagtagcg ggcactatt 
      ttacaagtg acttgtcaa aacagatac aaattagac 
      ggcgattcg atcattcta tgcgacaag ggacaatgg 
      tcctccgaa atcggcaaa tgtttacga acatgtcct 
      tcgatactc acgactaac aaactgcaa gcaacttgc 
      gttttcaaa ggcaaagaa tcggaaaat tgcacggaa 
      ccgatcgag ggtacgcaa gtcaagtac cagtgtgcc 
      ccatactat gaggataaa aacttggca caatatccc 
      gtcgggaac tgtaaagat ggtacctgg gaccgggca 
      acccccaat tgtgtcccg atctgtggt gtgaagtcc 
      gtccaaccc caacagttg ataatcggc ggaaacgtg 
      gtcaaaaaa ggggactat ccttgggtt gtggcgatt 
      tatcacgga cctcagaaa gatttcatt tgtagtggg 
      actctaata agtgaaaaa ataatccta acggcggca 
      ctctgtgta accaattcc ggcggtaaa gttcgggat 
      aagagtgat tttgcagtg gctgttgga aaatactat 
      caagaattt accgattcc cgcgaaaaa attagcaac 
      gttcaattc tctgatctt gaggcaatt cacgttcct 
      gattattac cgaggcctt gaccaaaat tactacggc 
      gatattgcc gttctggtg gcgaaaaaa agtttcacg 
      tttacgcaa acagttcag cctgtgtgt gtcgatttc 
      gcggcaaac tatgacaac aaaaatctt acaggattt 
      gtgacaggg tggggtttt acgaaggaa aatggaggc 
      gtatctgac gaactcaaa gagctcaaa gtgtcttta 
      gttagtgta gcggactgc aggaaagag ttgccctca 
      gagtttcaa aaataccta cttactgat aaaatatgc 
      gcaggatac agaaataaa ggattgtca gtttgtaaa 
      ggagacagt ggaggaggt ttagttgtc aagtcttca 
      actactggt agatacaac cttgctggt attgtaact 
      gtggctcca aacagtcct tcagggggc tgcaatagt 
      caacagtat gctctgtac acgacaatc tcccatcac 
      tacgaaaag tttctctca gggaaaata aacaatcgc 
      tag
    mRNA sequence
    • >TC031984 RA
      tcaacggga attccaaag aaatttcgg attactctc 
      acattatca catttcccc agctttgtg cgtctcttg 
      gacttttcg ataaaatat tataattaa cttgtagcc 
      gaggtcatc aaatcaaca tcaagaata ataatatgg 
      aacaaaatc agtgagatg ttgtgtgta acgtttgtg 
      cttgagcta gtttgttta tttacttcc gttaagtac 
      acaatagcc agtttcaca attttggaa tactccaat 
      tccaccttg cacagacat cggagacaa aatggttgc 
      gttttgcca caacatccg acaaacggc caatggtca 
      atttacaaa tcaaacgcc cgattttca cctggagaa 
      tccatacct tcgggcagt attttgcaa gttaagtgc 
      gaccaaacc tacaaacta gacggagac gctactatt 
      gtctgcatc aacgaaaaa tggacatcc gaagtaggc 
      gtttgcctg aaatgtggc caaaagtcg accagaaat 
      aacccgaca ctgatcgta aatgggact aatgcaatt 
      cggggagac tacccttgg caagtagct ttgtacaac 
      aagacgtcc aaagtgcta ctttgtgga ggctcttta 
      ctgaatcaa agagtgata ttaaccgct gctcattgc 
      ataacggac gacactggt aaattactg aataaagaa 
      acgtacaca gtggcagta ggcaaatac taccgcaaa 
      tatgaccac tcagaggac gtaaataaa gcgcaattt 
      tcggaaatc catgaaatg ttcacaacg aggaagtac 
      aaaggacac atacagaat tttttcggc gacattgct 
      atcatagtt acgaaaaaa atatttttc ctgtcagat 
      cgagtccaa ccggtttgc atagactgg gggttcaag 
      tatcaaaga aagcttctg aaccccaac gtgtctaaa 
      tatggctac gtgtctgga tggggctac accgccgaa 
      tacaaaaac ccctccgag gtactcaaa gaactgaaa 
      gtcccaata atcacaaac gaccagtgt gacaccgat 
      ttaccggaa gattaccaa aaattcatg actgatgat 
      aagatttgt gcgggattt ttaaatgct cgcacgtct 
      gtttgtagc ggtgacagt ggtggtgct ttagtaaca 
      gaatataaa ggtaggtac tacgctgtt ggaattgtg 
      agtctatcg ccacaagca ccaaccgaa gaaggtggt 
      tgtgatagt caacaatac accctcttc actagtgtt 
      tactactac atcaacgaa ctgattttt gaaaaagaa 
      gcccgatac agaccatca cattcggaa attgcgagt 
      tgcgaagaa gactccaaa tgtcaacct gaacccgtg 
      accacaact acgaccacg acaacgaca ccaaaacca 
      gtcacagaa ccaacaact ccaaattgc gttttgccc 
      gagcatccg gcctcaggc caatggtcg atctacggt 
      gtccaaact aaatttacc ccaggacag ttcgtatca 
      cccgcaaca gtgctggaa gtaacttgc cagaaagac 
      tacaaattg aacggaaac aacctcctg atttgccac 
      aacggcaag tggtcaccc gaaatcgga aaatgttca 
      aagacttgc cccccggtg caaagcacc ccttcaact 
      gtgataact tgcacttac aaaaacaac gaaactgac 
      tgcagcagt cctccggat ggtaccgtc gctaaatac 
      acctgtgcc cccttttat gagagtacc gaaacgacg 
      aaaaatccg gtcagggtg tgcaaaaac gggtcgtgg 
      gatttgtcc aagcctgaa tgcgttccg atttgtggg 
      aagaaatat gtgcgagca caatctttg atagtgaac 
      gggaggaaa gcaaaaggc ggcgaacat ccctggcaa 
      gtggctatt taccgaaat aacaaattt atttgtggt 
      ggagcttta attagcgaa aatcttata ctcactgcc 
      gcacattgc gtacgttat gagaaccgt gaagcaaac 
      gttgatgag tttactgtt ggcgttggc aagaattcg 
      attcgttta gacgacaca tctgatgtc cacgcacag 
      ttttctggg ttgcaggac atcatcgtc ccgaaacaa 
      ttcaatggg ctttacact aatttactg gcagatata 
      gcacttcta gttagtaaa aagaacttc acaatttcg 
      ttttctgta caaccggtg tgcctggat tgggctaat 
      tcgattaat ttgacacac aattccaag ggttacatc 
      tcaggttgg ggctacacg gctgaagga acaaaaccg 
      gctgatgat ttacaagaa ctggaagtg tcaattgtg 
      tcgaatcaa gactgttcc caactttta acggaagaa 
      ttaaaaccg ttcttgact ttcgataaa atatgtgca 
      ggggaccca actcacaat gccagtgcg tgcaatgga 
      gacgccggt ggtcctctc gttgtgaaa cacaacggt 
      aggttttac attaatgga attgtgagt agtgctgaa 
      gctgatcac accagtggc cggtgcaga agtgatcaa 
      tacggactt ttcatcaaa gtttcggaa taccaatcg 
      ttcattttg gaaactatg gccaaatac aatccacgc 
      tcaagcacc cccgaaata acatcaccc aaacctcca 
      ccaccaccc ccggaaaag cctgaaaat tactgcatt 
      ctaccaaat taccccgac tttggcaga tgggcactg 
      ttcgcgtcc ggcagtcat tcccccgga atgtcagta 
      gtagcgggc actatttta caagtgact tgtcaaaac 
      agatacaaa ttagacggc gattcgatc attctatgc 
      gacaaggga caatggtcc tccgaaatc ggcaaatgt 
      ttacgaaca tgtccttcg atactcacg actaacaaa 
      ctgcaagca acttgcgtt ttcaaaggc aaagaatcg 
      gaaaattgc acggaaccg atcgagggt acgcaagtc 
      aagtaccag tgtgcccca tactatgag gataaaaac 
      ttggcacaa tatcccgtc gggaactgt aaagatggt 
      acctgggac cgggcaacc cccaattgt gtcccgatc 
      tgtggtgtg aagtccgtc caaccccaa cagttgata 
      atcggcgga aacgtggtc aaaaaaggg gactatcct 
      tgggttgtg gcgatttat cacggacct cagaaagat 
      ttcatttgt agtgggact ctaataagt gaaaaaata 
      atcctaacg gcggcactc tgtgtaacc aattccggc 
      ggtaaagtt cgggataag agtgatttt gcagtggct 
      gttggaaaa tactatcaa gaatttacc gattcccgc 
      gaaaaaatt agcaacgtt caattctct gatcttgag 
      gcaattcac gttcctgat tattaccga ggccttgac 
      caaaattac tacggcgat attgccgtt ctggtggcg 
      aaaaaaagt ttcacgttt acgcaaaca gttcagcct 
      gtgtgtgtc gatttcgcg gcaaactat gacaacaaa 
      aatcttaca ggatttgtg acagggtgg ggttttacg 
      aaggaaaat ggaggcgta tctgacgaa ctcaaagag 
      ctcaaagtg tctttagtt agtgtagcg gactgcagg 
      aaagagttg ccctcagag tttcaaaaa tacctactt 
      actgataaa atatgcgca ggatacaga aataaagga 
      ttgtcagtt tgtaaagga gacagtgga ggaggttta 
      gttgtcaag tcttcaact actggtaga tacaacctt 
      gctggtatt gtaactgtg gctccaaac agtccttca 
      gggggctgc aatagtcaa cagtatgct ctgtacacg 
      acaatctcc catcactac gaaaagttt ctctcaggg 
      aaaataaac aatcgctag agttatttt aaaatttta 
      atcagcctg atttatttt ctatcgtaa ataaagtca 
      ttagtactg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY