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Gene information
General information
TC016394 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30116698..30127436
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016394
    iBeetle screen iB_06013

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016394 RA
      MPRCLMAKK WKAYPWPER PEQDDDHQD PEDEEIDVV 
      GDAPACWGP SSPTAGATA PSPPPTSPN GTTLIYNGY 
      LHEFSPSTY PPTTFIPRN DQPPSGQTQ PSYATLRTV 
      VETQDLRPD YSTPTPPRS RKSLAMTFT STGTALSLP 
      PKKKDIYRP YSLDDKPPP PPPPTIRVP AEEDLHAAH 
      AILDLSAST TVFLPPPPP PPPPERNDE DNVLHPAPV 
      KSGKTIAYT YEAFFVSDG RSKKRFTPD ETLTAKALV 
      NEKPKYTCS ECGKQYATS SNLSRHKQT HRSLDSQSA 
      KKCITCGKA YVSMPALAM HVLTHKLAH RCSVCGKQF 
      SRPWLLQGH LRSHTGEKP YGCAHCGKA FADRSNLRA 
      HMQTHSADK NFECPRCHK TFALKSYLN KHLESACLK 
      EEAGPSSCA EEVAREVPV PIRVYAA
    CDS sequence
    • >TC016394 RA
      atgccgcgc tgtctcatg gccaagaag tggaaagcg 
      tatccgtgg ccggagcgc cccgagcag gacgacgac 
      caccaggac cccgaggac gaggagatc gacgtcgtc 
      ggcgatgcg cccgcctgc tgggggccc tccagtcct 
      acggccggc gccacggcg cccagtccg ccgccgacg 
      tctccgaac ggaaccacg ctcatctac aacggctac 
      cttcacgaa ttctctcct tccacctat cccccaacc 
      accttcatc ccccgtaac gaccaacct ccaagcggc 
      caaacgcaa ccatcttac gcaacctta cgaacggtg 
      gtggaaacc caagacctt cgtcccgac tacagcact 
      cccactcct ccccgttcc cgcaaatct ctcgccatg 
      accttcacc agcacggga acagccctc tccctcccg 
      cccaaaaag aaggacatc taccgtcct tactccctc 
      gacgacaaa ccaccgccg cctccaccc cccaccatc 
      cgggtaccc gccgaggag gacctccac gccgcccac 
      gccatcctc gacctcagc gcctccacg acggtgttc 
      ctgccgccg ccgccgccg ccaccacct ccagaacgt 
      aacgatgaa gacaacgtc ctccacccg gcaccggtg 
      aagagcggc aaaacgata gcctacact tatgaagct 
      tttttcgtc agtgatggg cgttccaag aaacggttt 
      actccagac gagaccttg acggctaag gctcttgtt 
      aacgaaaag cccaaatac acttgcagt gagtgtggg 
      aaacaatac gcaacttcg agtaacttg tcacgtcac 
      aagcaaact cacaggagt ttggactcc caatcggcc 
      aagaaatgt ataacctgt gggaaggct tacgtcagt 
      atgcctgcc ttggctatg catgtgctt acgcacaaa 
      ttggctcat agatgtagc gtctgtggt aagcaattc 
      tcgcgacct tggttgctc caaggtcac ttaaggtca 
      cacacgggg gagaagccc tacgggtgt gcccactgt 
      ggcaaggcg tttgccgac aggtcgaac ctaagggcc 
      cacatgcag acgcactca gctgataaa aatttcgag 
      tgccccagg tgtcacaaa acattcgcg ctgaagtcg 
      taccttaac aaacacctg gagtcggcg tgtcttaag 
      gaggaggcg gggcctagc agctgcgcg gaggaggtg 
      gccagggag gtgcctgtg cccatacgc gtctatgcg 
      gcctag
    mRNA sequence
    • >TC016394 RA
      gccacgagg ggtctgttt agtttagtt ctgttgtgt 
      tcgctcttt cggtgctct gcctgtctt ctctcctgt 
      cgtctgtcc tctcgttct tccgttcga tgtcgatga 
      tgtcttcgc gacggtggt ggtgcgagc agtgcgcga 
      gtgcgcccc tctagggat ggacggtga tgacgcgaa 
      gagggctcg ccgtcgccg tcgccgact agtcacgcg 
      atagcgcca tgccgcgct gtctcatgg ccaagaagt 
      ggaaagcgt atccgtggc cggagcgcc ccgagcagg 
      acgacgacc accaggacc ccgaggacg aggagatcg 
      acgtcgtcg gcgatgcgc ccgcctgct gggggccct 
      ccagtccta cggccggcg ccacggcgc ccagtccgc 
      cgccgacgt ctccgaacg gaaccacgc tcatctaca 
      acggctacc ttcacgaat tctctcctt ccacctatc 
      ccccaacca ccttcatcc cccgtaacg accaacctc 
      caagcggcc aaacgcaac catcttacg caaccttac 
      gaacggtgg tggaaaccc aagaccttc gtcccgact 
      acagcactc ccactcctc cccgttccc gcaaatctc 
      tcgccatga ccttcacca gcacgggaa cagccctct 
      ccctcccgc ccaaaaaga aggacatct accgtcctt 
      actccctcg acgacaaac caccgccgc ctccacccc 
      ccaccatcc gggtacccg ccgaggagg acctccacg 
      ccgcccacg ccatcctcg acctcagcg cctccacga 
      cggtgttcc tgccgccgc cgccgccgc caccacctc 
      cagaacgta acgatgaag acaacgtcc tccacccgg 
      caccggtga agagcggca aaacgatag cctacactt 
      atgaagctt ttttcgtca gtgatgggc gttccaaga 
      aacggttta ctccagacg agaccttga cggctaagg 
      ctcttgtta acgaaaagc ccaaataca cttgcagtg 
      agtgtggga aacaatacg caacttcga gtaacttgt 
      cacgtcaca agcaaactc acaggagtt tggactccc 
      aatcggcca agaaatgta taacctgtg ggaaggctt 
      acgtcagta tgcctgcct tggctatgc atgtgctta 
      cgcacaaat tggctcata gatgtagcg tctgtggta 
      agcaattct cgcgacctt ggttgctcc aaggtcact 
      taaggtcac acacggggg agaagccct acgggtgtg 
      cccactgtg gcaaggcgt ttgccgaca ggtcgaacc 
      taagggccc acatgcaga cgcactcag ctgataaaa 
      atttcgagt gccccaggt gtcacaaaa cattcgcgc 
      tgaagtcgt accttaaca aacacctgg agtcggcgt 
      gtcttaagg aggaggcgg ggcctagca gctgcgcgg 
      aggaggtgg ccagggagg tgcctgtgc ccatacgcg 
      tctatgcgg cctagtcgc attttttcc gtctttcct 
      ggcaagtac cgaagggat cgatattag acgatccct 
      cctgtactt tttcccaag ttagtgtag tttgtaatc 
      gcggcacgt agtggcagt gttccgtca cgccgccta 
      gattagccg tgatttatt cttcgcgaa ctgacgccc 
      ccttgtaaa tatccctcc caaagagat aacacatcg 
      caacacgtc caataagtt agggttaga tgtaagatg 
      gtctctacc tttagaaca ccatggacg cggaacgcg 
      tccaccgaa cagatgtta tcggcaccc tttagttaa 
      ttaaatcgc gacattcca aacgttctt cacgaacaa 
      aagcaaagt ttcacaaac aacgaagtt atgcaaata 
      ctagtatta tacatattt agattaaga gtggaatta 
      agtgagatt taaatatta taatgagcg gccggagcc 
      gcatctagt cgcagcctc tagtcccgg acctggtta 
      atccattct agtcccagt tatagtcaa ttatcgttt 
      cggttcggt ttgttgttc agatctata tgatattat 
      ataccgcgc ggagtctaa agcacgact tagccaacg 
      acttttatt ccaattgta ctatatata catagcgaa 
      caagagaaa attattaaa aaaaataat tgagatcaa 
      cgaagcata gttttggca gtatttttg caagcatat 
      attatacat gataactat atatttatt aatacggta 
      atgaccttg tcaataccg acgtctagg taaacgaag 
      gagtagcac ctataacct gttgtccct gattgttac 
      gagtgggcc agtgcaatg aaaaacaac caaaatgca 
      aacaagaca aaaaccaac aaaatctac actagtcaa 
      ttacagtca attataatc ttagatgtt cttctaatt 
      ttaattcaa aattctata aaaaatgtt aagacttta 
      accaaattt tttattaat aataatata caaagcgtg 
      tcagaacta agcaacgta tccggctcg aagaaccat 
      attttgttg ctcaaatta caatttaat aatgatatt 
      aaaaaaata aagttggta ttgtgtaat tcattttgt 
      cagaaatta ttatgacaa atgggcaaa gttttacag 
      taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY