| General information | |||
| TC033672
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 6850325..6934015 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033672 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06009 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC033672 RA
MQGETTKHY LYSTVNSFS DITVQHLRA FLPQQIQRI VILDSLVVT QDIEAVEGK SVSLPCDVT PPNNHDKVY MVFWFQQGS GYPIYSFDV RGKPLSRAS HWSAPDLFG SRAYFRTVS EPAQLVIDD IRRHDEGVY RCRVDFRNA QTRSFRYNL TVIVPPEQP TILDKWGRQ LNGTVGPHE EGDDITLTC RTVGGHPQP TVRWLINAE MVDEQSEHN AGDVIENRL VKTSISRKD LDAIFTCQA VNTVLTEPK VASVVLDLL LKPLTVRIL KTVSPLVAD KRYEVTCES AGSRPPAII TWYKGKRPL RKTKQETRE NVTISELTF IPTTEDDGK SITCRAENP NVTGLFLET SWKIDVVYP PQVSLHLGS TLNADDIKE GDDVYFECR VTANPQWRK LTWLHNGFV LNHNMSGRI IHSNQSLVL QKVTRQSAG RYVCSVVNS EGETLSNEL NFRVQYAPT CKFDKFLVV GASRGESVD IVCEIESDP PAKSYRWKF NNSGETLDV AAERFAKTS NGTVSVLRY TPVSELDYG SLSCWAINT VGHQVNPCV FQVVAAGKP FPVRNCSLS NQTSSSVEV TCLAGFDGG LPQHFLLEL YTANSLVPR YNMTSFRDP EFFLDNLEP DVTFRIVVF AVNAKGRSQ GVVLEEVTF TDAEKRTAS DGDLPFSPI LGILIGATL TIIIVILVV IIRVRRSQS QPEVTIEQK VLGAPHHNT MSSAKPLLR SSSPRDTDE KDPDVIPAK YGSPDGECA RNLGSLNGP QSLPLGSLA TSPNYAQTA VPGPQWVSP VAPTTEPMY HLYNNPVAM VGTFSRDSR PKELRLGNG SSATLQRAT DLELNGLAI KERLMANRL PESCV
- >TC033672 RA
atgcaggga gaaacaacc aaacactac ctatattcg acggtaaat tcattttct gatattaca gtgcagcac ttgcgagct tttttgccc cagcaaata cagcgcata gtaatatta gactctttg gtcgtgact caagacatt gaagctgtc gaaggaaaa agtgttagt ttaccgtgt gatgtgacg ccgccgaat aaccatgat aaagtatac atggtcttc tggttccag caggggtcg gggtacccc atatacagc ttcgacgtt cgtggcaag cctctgtcc agagcgagt cattggtcc gcaccggat ctgttcggc tctagagcc tacttccgg acggtgtct gagccagca cagctcgtc attgacgac atccggcgt cacgacgaa ggcgtctac cggtgcaga gtggacttc cgcaatgct cagacccgc agcttccga tataatctc accgttatt gttccgccg gagcagccc acaatcctg gacaagtgg ggccgtcag ctgaacggc actgtggga ccacacgaa gagggcgat gacataact ctgacttgt aggacagtc ggggggcac ccccagccc acggttcgt tggttgata aacgcggaa atggtcgac gagcagtcg gagcacaac gcaggggat gtcattgag aaccggctg gtgaagact tcaatttcg aggaaggat ttggacgct atcttcacg tgtcaggct gtcaacacg gttctcacc gagcccaaa gtggcctca gtcgtgctg gatttgcta ctcaagcct ctgacggtc cgtatcttg aaaacggtt agccctctt gtggccgac aagcgctat gaagtcacc tgcgagtct gcagggtct cggccgccg gcgatcatc acctggtat aaaggcaaa cgccccttg cgcaagaca aagcaagaa acgcgagag aatgtaaca ataagtgag ttaactttc attccgacg accgaggac gatggaaaa tccataaca tgcagagct gaaaatccc aacgtcaca ggcctcttt ctcgaaaca tcgtggaaa attgacgtc gtttacccc ccgcaagtg tcgctccat ttggggagc acgcttaat gctgatgac atcaaggaa ggcgacgac gtctacttc gagtgtcga gttaccgcc aacccccag tggaggaaa ctcacttgg ctgcacaac ggcttcgta cttaaccac aacatgtct ggtcgcatc atccacagc aaccaaagt ttagttctc caaaaggta accagacaa agtgccggc cgatatgtc tgctcggtg gtgaacagc gaaggagag acgctcagc aacgagctc aactttcgc gtacaatac gctccaact tgcaaattc gataaattt ctggtggtg ggggcttcc agaggcgaa agcgtggac atcgtctgc gaaatcgag tcggaccct cccgctaag agctaccgg tggaagttt aacaactcg ggagagacg ctcgacgtc gctgcagaa cgctttgca aagaccagt aatggaaca gtgagcgtt ctaaggtac acaccagtg tctgaactg gattacggg tcattatcg tgctgggcc atcaacacg gtgggccat caagttaat ccctgcgtt ttccaagtt gttgcagca ggcaagccg ttccccgta cgaaactgc tctttgtct aaccaaacg agcagttcg gtggaggtg acatgtctc gctggcttt gacggcggt cttccgcaa cactttctt ctggagttg tacacggca aactcgctc gttccgcgc tacaacatg acgtcgttc cgagacccg gagttcttc ctcgacaat ttagagcca gatgttacc ttccgaatt gttgttttc gccgtcaac gcgaaggga cgcagccag ggagtggtg ctggaggaa gtcaccttc actgacgcc gaaaagcgg acagcttcg gacggcgac ttgcccttc tcccccatc ctggggatc ctaatcggg gccaccctc accataatc attgtgatc ctcgtggtg ataattaga gtccggagg tctcaaagc cagcccgag gtcacaata gagcaaaaa gtattaggt gctcctcac cacaacaca atgtcttct gcaaagcca ctcttgagg agctcctct ccacgtgac accgacgag aaggatccc gatgtaatt cccgctaaa tacggctct cccgacgga gagtgtgcc aggaaccta gggtcgctg aacggtccc caaagtctt cctctgggc tcccttgcg acgtctccg aattacgct cagacagct gttccgggc ccccaatgg gtcagtccc gtcgctccc accaccgag ccgatgtat catctgtat aataatcct gtggcgatg gtgggcacg ttttctagg gattccagg ccaaaggaa cttcgttta ggaaacgga agctcggcc acgcttcaa agggccaca gatttggag ttaaacgga ttagccatt aaagagaga ctgatggcg aaccggcta ccggagagt tgtgtttaa
- >TC033672 RA
tgttaaaag attaataaa atccatata aatcattgg tttccagta atttcacga aattggaat taaaatttt cgcagttat ctgatgtgt ggttgaaac aatgtttga ttatcgtat tattacatg ttttgcggt tggagtcgt aaatgtaga gaagagttt agtggtgta aatgtaaat ttacacaag gataagatt actcggcgc tccataaaa tccggttgt aataaagtg acataatta aaatataat tgaaaatcg ttacattct cgataaatg cgccgttat ggccactac acatgcaat attttaatt tgttgtaaa atacggttt taaatgtaa tttaagtca taaatccgg attaagttg tgaataatt ctcgtgtaa tgtaattaa atttcgtga aatggttgt atttacttg tactctaat ttgaaaaag tccgttcta aataatgaa aagtattgt ttgtcagtt tattctgtc ccttttatt caaatgaaa gcttgtgaa tttaaaatt gtatttttt acaaaaatc ttttcagga ttattttta tgaatgaat atcattctt tgcatacaa aagaaacac tggaacaaa acaaattac ccacaggcc attcatact ttattcagt tattgaaat tctgaatag gttttgtga gcggaaaat ctaagacga tttttcttt ttttttgag cgaaatcac ttaattttt tgcagtggt gacagcgaa ggaaatccg tgtctgtgt aaaatgtgg cagtcaaca tgtgtttgt tgttaaaaa tcagacaag caattctcg tgggttgca actgcaatg aattttaca acgtcccga gctcatgtg aaatttaaa aagaagcaa tctataatt tgtgctgca gcagttttc atttccaat ccggctgaa tcagtgaca aaatacgca atctatttt tccgatgat aaatagcga atatctagt acaacgtaa tgaacgaat atggcatca actttataa atggaacgg aatccagaa gcagtccct gtggaagtc ctcgcatag ccaccacta tgaactcat atatcatgc agagtgttc gttgtttta attaagagc accatgaat tcgaatatg gatctctgt ataaatatt cataaatta tgaacgatc gagccggcc ctaattgca tttttccct taagcttgt caataataa aattaccat taatcattg tctcaagtc tttgctgtt aaacgaatt tgttttcga ttaaaattc aattgcttt ttgaggttt attaatcag cggtacctg ccgttaatt aacttgtta ttcaaacgc atgcatgcc gggaattaa aaccgcaaa aagattaac acactgcac actgtttga ttctcgtta caggttaat ttttaatta aaaagtcaa caagattcg aggcaaatg cagggagaa acaaccaaa cactaccta tattcgacg gtaaattca ttttctgat attacagtg cagcacttg cgagctttt ttgccccag caaatacag cgcatagta atattagac tctttggtc gtgactcaa gacattgaa gctgtcgaa ggaaaaagt gttagttta ccgtgtgat gtgacgccg ccgaataac catgataaa gtatacatg gtcttctgg ttccagcag gggtcgggg taccccata tacagcttc gacgttcgt ggcaagcct ctgtccaga gcgagtcat tggtccgca ccggatctg ttcggctct agagcctac ttccggacg gtgtctgag ccagcacag ctcgtcatt gacgacatc cggcgtcac gacgaaggc gtctaccgg tgcagagtg gacttccgc aatgctcag acccgcagc ttccgatat aatctcacc gttattgtt ccgccggag cagcccaca atcctggac aagtggggc cgtcagctg aacggcact gtgggacca cacgaagag ggcgatgac ataactctg acttgtagg acagtcggg gggcacccc cagcccacg gttcgttgg ttgataaac gcggaaatg gtcgacgag cagtcggag cacaacgca ggggatgtc attgagaac cggctggtg aagacttca atttcgagg aaggatttg gacgctatc ttcacgtgt caggctgtc aacacggtt ctcaccgag cccaaagtg gcctcagtc gtgctggat ttgctactc aagcctctg acggtccgt atcttgaaa acggttagc cctcttgtg gccgacaag cgctatgaa gtcacctgc gagtctgca gggtctcgg ccgccggcg atcatcacc tggtataaa ggcaaacgc cccttgcgc aagacaaag caagaaacg cgagagaat gtaacaata agtgagtta actttcatt ccgacgacc gaggacgat ggaaaatcc ataacatgc agagctgaa aatcccaac gtcacaggc ctctttctc gaaacatcg tggaaaatt gacgtcgtt taccccccg caagtgtcg ctccatttg gggagcacg cttaatgct gatgacatc aaggaaggc gacgacgtc tacttcgag tgtcgagtt accgccaac ccccagtgg aggaaactc acttggctg cacaacggc ttcgtactt aaccacaac atgtctggt cgcatcatc cacagcaac caaagttta gttctccaa aaggtaacc agacaaagt gccggccga tatgtctgc tcggtggtg aacagcgaa ggagagacg ctcagcaac gagctcaac tttcgcgta caatacgct ccaacttgc aaattcgat aaatttctg gtggtgggg gcttccaga ggcgaaagc gtggacatc gtctgcgaa atcgagtcg gaccctccc gctaagagc taccggtgg aagtttaac aactcggga gagacgctc gacgtcgct gcagaacgc tttgcaaag accagtaat ggaacagtg agcgttcta aggtacaca ccagtgtct gaactggat tacgggtca ttatcgtgc tgggccatc aacacggtg ggccatcaa gttaatccc tgcgttttc caagttgtt gcagcaggc aagccgttc cccgtacga aactgctct ttgtctaac caaacgagc agttcggtg gaggtgaca tgtctcgct ggctttgac ggcggtctt ccgcaacac tttcttctg gagttgtac acggcaaac tcgctcgtt ccgcgctac aacatgacg tcgttccga gacccggag ttcttcctc gacaattta gagccagat gttaccttc cgaattgtt gttttcgcc gtcaacgcg aagggacgc agccaggga gtggtgctg gaggaagtc accttcact gacgccgaa aagcggaca gcttcggac ggcgacttg cccttctcc cccatcctg gggatccta atcggggcc accctcacc ataatcatt gtgatcctc gtggtgata attagagtc cggaggtct caaagccag cccgaggtc acaatagag caaaaagta ttaggtgct cctcaccac aacacaatg tcttctgca aagccactc ttgaggagc tcctctcca cgtgacacc gacgagaag gatcccgat gtaattccc gctaaatac ggctctccc gacggagag tgtgccagg aacctaggg tcgctgaac ggtccccaa agtcttcct ctgggctcc cttgcgacg tctccgaat tacgctcag acagctgtt ccgggcccc caatgggtc agtcccgtc gctcccacc accgagccg atgtatcat ctgtataat aatcctgtg gcgatggtg ggcacgttt tctagggat tccaggcca aaggaactt cgtttagga aacggaagc tcggccacg cttcaaagg gccacagat ttggagtta aacggatta gccattaaa gagagactg atggcgaac cggctaccg gagagttgt gtttaaaac aagtgtctt ttgtatcgt ttggtcgta cgcaaaatt actttaagg accgtccaa agcgttacg gcaatggcg aggggactg catttaaca aaaaacact accatatca ctttgccaa attgttgcc accagtcaa ctttggaca ctggataag tactggtgg caatttttt ccgattttt taaaccatg ttcctcgct gcatccgac tttccgcca aactcgcca actgccgtt gcacaaaga gttatcgtt ttctaaatg taaattgtg tctaaagat agactgagt tagctaagc gatgtaaat atgtttaaa tctgagtgt tcgtgttat cggtggaag agtggacac ctcatacag acaatccgt agctgaacc taactaaat ttctatgaa gacttgtaa atatcattg ttgcatatc gtgcagatg gttggttgt tttgtaacc agtaactga atgcttttt gataaaaaa ttaacatac gaaataagt gaaacgaat ttgattatc tagagtgtt ctattgctc acattttgc tagaaagtt tatctcgtt tatcaccaa attgtttga gtaattttg tttgccaaa attggcgtg tcttccatg tccctacgg tttgatttg agtagagtc ggacaatgt gaacaataa aaccgcaat gaggatgaa caaggaata agtgttttg ttaaatttt tgagaaact cgattcgtt tctgtgtac atgtagata tcaaaaa
