Status
Please wait ...
Gene information
General information
TC016363 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7054157..7092107
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016363
    iBeetle screen iB_06007

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016363 RA
      MYTPKLSQM DISENSTYL FDKHEDRNN TDRDENLAR 
      VEVATLAII FLVTVIGNS TVLLALWTR RRYAGRKKL 
      SRMYFFILH LSIADLITA FLSVLPQLA WDITYRFYG 
      GFLLCKVVK YGQTLGPYL SSYVLMATA IDRHQAICY 
      PLTYCSWTS RRSKVMVYL AWVASLAFC IPQLTIFTY 
      TSVGEDEYD CWATFQEPW GKRAYVTWY SISVFMVPL 
      VVLIFTYTS ICIEIWQSS ESSLRPRSS QKSAPGKRT 
      PLISRAKIN TVKQTIAVI VMYIACSTP FILAQLWAT 
      WDPQSPFID GPVFVILTL LYSLNSCVN PWIYLAFNR 
      ELPRLLLRH YTASSKNYR SATGGNSAS NSSGDAQST 
      SLRPFSRWS LCNSARSNK YPTRVPHRP YVAQYNARR 
      WIVTTTT
    CDS sequence
    • >TC016363 RA
      atgtacacc ccgaaactt tcccaaatg gacatctct 
      gagaactct acgtatctc ttcgacaaa cacgaagac 
      agaaacaac accgatcgg gacgaaaac ctcgcccgc 
      gtggaggtt gccaccctt gccatcata tttcttgtc 
      accgtcatc gggaacagc acagtatta ctggcttta 
      tggacccgc cgaagatac gccggtcgc aagaaactc 
      tcccgaatg tacttcttt atcttacat ctgagcatc 
      gccgatttg atcacggct tttctcagc gtactgccg 
      cagctggcc tgggacata acttataga ttctacgga 
      ggtttcctt ctctgtaaa gtcgttaaa tatggacag 
      actttaggt ccgtatctg agctcgtac gtgctgatg 
      gcgacggcc attgatcgc catcaggcc atttgttac 
      cctttgacc tactgctcg tggacttcc aggaggtcg 
      aaagttatg gtgtatctg gcgtgggtg gccagcctc 
      gccttctgt attccacag ctgacgatc ttcacatac 
      acgagcgtc ggagaagac gaatacgac tgctgggcc 
      actttccaa gaaccatgg ggtaaaaga gcttacgtc 
      acatggtac agcatttct gtgttcatg gtgccgttg 
      gtggttcta attttcacg tatacgtcg atttgtatc 
      gagatttgg caaagctcg gaatcgagt ctgcgacca 
      cgaagctct cagaaatca gcacctggg aaacgtacc 
      cctttgatc tctcgagcg aagattaat actgtcaag 
      cagactatt gccgtcatt gtaatgtat atagcgtgc 
      tcgactccg ttcattcta gcacaacta tgggccact 
      tgggacccc caaagcccc ttcatcgac ggcccggtg 
      tttgtaatc ttaacgctc ctctactcg ctaaacagc 
      tgtgtaaat ccttggatt tatctggca ttcaaccgg 
      gagttgccg cgcctcctg ctccgtcac tacacggct 
      tccagcaag aactaccgt tctgcgacc ggaggtaac 
      agtgcaagt aactcctca ggtgacgcg cagagcacg 
      tcattgcgg ccgttctcg cgctggtcg ctctgcaac 
      agcgccagg tccaacaag taccccacg agggtgccg 
      cacaggccc tacgtcgcc cagtacaac gcccgccgg 
      tggatcgtc acgacgacc acctga
    mRNA sequence
    • >TC016363 RA
      cgcaagcac cgaagagaa gttgaatgg acttgcgat 
      gcgacgcgc cagtgaaga aacagtgcg aatttttac 
      taacttttg aattaaaca agaacaaga cagtaaaat 
      cgcagttta aggacttag aaatccgag tgttggtgg 
      atttttata tcgaaaagt ttcaacaac tggaaactt 
      cgctgaaag accgaaata atttcaaaa tagtctcat 
      gtacacccc gaaactttc ccaaatgga catctctga 
      gaactctac gtatctctt cgacaaaca cgaagacag 
      aaacaacac cgatcggga cgaaaacct cgcccgcgt 
      ggaggttgc cacccttgc catcatatt tcttgtcac 
      cgtcatcgg gaacagcac agtattact ggctttatg 
      gacccgccg aagatacgc cggtcgcaa gaaactctc 
      ccgaatgta cttctttat cttacatct gagcatcgc 
      cgatttgat cacggcttt tctcagcgt actgccgca 
      gctggcctg ggacataac ttatagatt ctacggagg 
      tttccttct ctgtaaagt cgttaaata tggacagac 
      tttaggtcc gtatctgag ctcgtacgt gctgatggc 
      gacggccat tgatcgcca tcaggccat ttgttaccc 
      tttgaccta ctgctcgtg gacttccag gaggtcgaa 
      agttatggt gtatctggc gtgggtggc cagcctcgc 
      cttctgtat tccacagct gacgatctt cacatacac 
      gagcgtcgg agaagacga atacgactg ctgggccac 
      tttccaaga accatgggg taaaagagc ttacgtcac 
      atggtacag catttctgt gttcatggt gccgttggt 
      ggttctaat tttcacgta tacgtcgat ttgtatcga 
      gatttggca aagctcgga atcgagtct gcgaccacg 
      aagctctca gaaatcagc acctgggaa acgtacccc 
      tttgatctc tcgagcgaa gattaatac tgtcaagca 
      gactattgc cgtcattgt aatgtatat agcgtgctc 
      gactccgtt cattctagc acaactatg ggccacttg 
      ggaccccca aagcccctt catcgacgg cccggtgtt 
      tgtaatctt aacgctcct ctactcgct aaacagctg 
      tgtaaatcc ttggattta tctggcatt caaccggga 
      gttgccgcg cctcctgct ccgtcacta cacggcttc 
      cagcaagaa ctaccgttc tgcgaccgg aggtaacag 
      tgcaagtaa ctcctcagg tgacgcgca gagcacgtc 
      attgcggcc gttctcgcg ctggtcgct ctgcaacag 
      cgccaggtc caacaagta ccccacgag ggtgccgca 
      caggcccta cgtcgccca gtacaacgc ccgccggtg 
      gatcgtcac gacgaccac ctgaaatac ggagaccgg 
      tggtctgcc agaccacga catcgacga tatttccgg 
      ctcctcgct gctggaaat cagcgagct gacgaccga 
      attgacaga aatggaatc tgtgatgtg acagcacga 
      cgatgctac ttgtacctg acctcaagg aactattta 
      tttgtaatt ttgcgtgtt ttcgagttt taaatagta 
      cgaaagtcc acttctgtc cactcccat acgtgtaat 
      tttgtactt ttgatgtga tacttgtta actccgaag 
      atacattat tcttgtttc tgggttact caaatgttg 
      tgagatttc atattttat aaggaaata ttatataat 
      ttgctgtaa tatttttct aaataaatt caacttgca 
      tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY