| General information | |||
| TC032556
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 20453254..20465297 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032556 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06002 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC032556 RA
MLKLQYTQK FRDAWLKDP ILKDWLVVV ESIAGKEAK CKFCSKIVT SRYADLKAH GESKKHKTI AETAIGKPK ICDHVVGTA VWGNMAKLG EVGELRDNI RKALVSSVR TLFNILFGT GQRLNRTKI CDFEGFGWE VDSDECRQK IENLKRGYS VAELTAVCV LLGISYDGT AEDLATRLC TALTDFSAL SAVAEVEDD EEEDIHTVA NDTKVATGN RPASRQQIM PQTPVPNFS ISFRDVEDS IRPFSGTDP AYPVEKFVA DFEDLAMLL GWGDLQSYD LKTRLVREF AAKINSAEL HQQLRERKM KKSESAYEY FLLMKELAS RGNIEEEAV VHYIVEGII DDSENKLML YEASSYDQL RIKLEVYEK YKRSKNLKI AGSCRGEYQ GVKEKPKTS AFAEDKKHL GPIPRKCFN CSLIGHVAA QCPKPKREK GSCYGCGKL DHVVANCPD RKRKERPIE RSETARNHA MLVEKSGRD CEVAVDLEL GDIAKNLLG VVDSAAPKA NGQIERVNR VLTPMLAKL TVELNKWDK SLPQLELVL NNAANRSTG YSPSKLLFG VNQSYLEDD NIKTYLESI QKDSRDLLT IRAEASTKM ELVGKYNKD QFDNRHKSP RKYQKSCDH VVGTAVWGN MAKLGEVGE LGDNIRKAP VSSARTLFN ILFGTGQRL NRTKICDFE GFVWEVDSD ECQQKIENI KRGYSVAEL TAVCVLLGI SYDGTAEDL ATRLCIALT DFSALNAVA EVEDDEEED IHTVANDTE VATGNRPAS RQQIMPQTP VPNFSISFR DVEDSIRPF SGTDPAYPV EKFVADFED LAMLLGWGD LQSFLFAKK SLIGLAKMF VQGLHGVRT WYDLKTRLV REFAAKINS AELHQQLRE RKMKKSESA YEYFLLMKE LASRGNIEE EAVVHYIVE GILDDSENK LMLYEASSY DQLRIKLEV YEKFKRSKN LKIAGSSRG EYRGVKEKP KTSAFAEDK KHLGPIPRK CFNCSLIGH VAAQCPKPK REKGSCYGC GKLDHVIAN CPDRKRKER PIERSETAR NHAMLVEKS GRDCEVAVD LELGDIAKN LLGVVDSGS PISFIKSFL IPHNLRKPY LDTQCYTGI NSSKLIILA LYEGTVFVN SIKTSILFH VVPDETMKY DVALGRNFL FTSGLKISF DDNNMIIEQ KPNERRESF DNELLLIDT TVFEGKELD CVINPELPR ETLQKVENL VYEYNLPKV NDTQGTPTD FQTKIILRP DHTPFNFYP RRLSFYEKT QLQKILDDL QREGIIKES NSPYASPIV LVPKKSGEL RLKKS
- >TC032556 RA
atgctaaaa ctacagtat actcaaaag ttccgcgac gcatggttg aaggatcct attttgaaa gactggcta gtggttgta gaaagcatt gcagggaaa gaggcaaag tgtaaattt tgttcaaaa attgtcact agtagatat gcggatttg aaggctcat ggagaatcc aagaaacac aagacaatc gctgaaact gccatagga aagccaaaa atttgcgac cacgtggtg ggtactgcc gtgtgggga aatatggcg aaactggga gaagtgggt gaattaaga gacaacatt aggaaggct ctggtcagt tcggtgcgt acgcttttt aatattcta ttcggaacg ggccaaaga ctaaatcgt acaaaaatt tgtgacttt gagggtttt gggtgggag gtagactcg gatgaatgt cgacagaaa attgaaaac ctaaaaagg gggtacagt gtcgcggaa cttacggcg gtttgcgtg cttttgggt ataagttac gatgggacc gcagaggac ctcgcgacg cggctgtgc accgcatta acagacttt agtgccctc agtgccgtt gcggaagtg gaggatgac gaagaggaa gacatccat accgtagct aatgataca aaagtggcg acgggcaat cgacctgct tcgagacaa caaatcatg ccgcagacc ccagtgccg aatttttcg atatcgttt agagacgtg gaggattcg atacgaccc ttttcaggc acagacccg gcgtatcca gtcgaaaag ttcgtggct gattttgaa gatttggca atgttgttg ggatgggga gatttgcaa tcatacgac ctcaaaact cgattagtg cgagagttc gcggccaaa ataaacagc gcggagttg catcaacaa ttacgggaa agaaaaatg aaaaaaagt gaatctgcg tatgaatat tttcttttg atgaaggag ttggcttca cggggcaat attgaagaa gaagcggtg gtgcactac atcgtagaa ggaatcata gatgacagt gaaaacaaa ttaatgttg tacgaggcg tccagctac gaccaatta cgaattaaa ttagaagtc tacgaaaaa tataagcga tcgaaaaat ctaaaaatt gccggatcc tgcagagga gagtaccag ggggttaag gaaaaaccg aaaacgtcc gcatttgcc gaagacaaa aagcatttg ggcccaatt ccaaggaaa tgttttaat tgcagcctt ataggacac gtagcggcc caatgtccg aaaccgaag agggagaaa ggttcttgt tacggctgt gggaaactg gaccatgtc gtagcaaat tgtcccgat agaaaaagg aaagagagg cccattgag cgaagcgaa acggcaagg aatcacgca atgcttgtt gagaagtcg gggagagac tgcgaagtt gcggtggac cttgaatta ggtgatatt gctaaaaat cttttaggt gtagttgat tcagcggct cctaaagcg aatggacaa atagaaaga gttaatcgc gtattaaca cccatgctt gccaaactg acggtagag ttgaataaa tgggacaaa agtttaccg caactcgaa ctagttcta aataatgca gcaaatcgt tccacgggg tattcccca tcaaaatta ctatttggg gtcaatcaa agctacttg gaggatgat aatattaaa acgtattta gagtctata caaaaggat agtcgtgat ttacttaca attagggcc gaagcaagt acgaaaatg gaattggtc ggtaaatat aataaagac caatttgac aaccgtcat aagagtcct agaaaatac cagaaaagt tgtgaccac gtggtgggt actgccgtg tggggaaat atggcgaaa ctgggagaa gtgggtgaa ttaggagac aacattagg aaggctccg gtcagttcg gcgcgtacg ctttttaat attctattc ggaacgggc caaagacta aaccgtaca aaaatttgt gactttgag ggtttcgtg tgggaggta gactcggat gaatgtcaa cagaaaatt gaaaacata aaaaggggg tacagtgtc gcggaactt acggcggtt tgcgtgctt ttgggtata agttacgat gggacagca gaggacctc gcgacgcgg ctgtgcatc gcattaaca gactttagt gccctcaat gccgttgcg gaagtggag gatgacgaa gaggaagac atccatacc gtagctaat gatacagaa gtggcgacg ggcaatcga cctgcttcg agacaacaa atcatgccg cagacccca gtgccgaat ttttcgata tcgtttaga gacgtggag gattcgata cgacccttt tcaggcaca gacccggcg tatccagtc gaaaagttc gtggctgat tttgaagat ttggcaatg ttgttggga tggggagat ttgcaatca tttttattt gcaaaaaag tcgttaatc gggttagcc aaaatgttt gtccagggt ttacacgga gtaaggacg tggtacgac ctcaaaact cgattagtg cgagagttc gcggccaaa ataaacagc gcggagttg catcaacaa ttacgggaa agaaaaatg aaaaaaagt gaatctgcg tatgaatat tttcttttg atgaaggag ttggcttca cggggcaat attgaagaa gaagcggtg gtgcactac atcgtagaa ggaatctta gatgacagt gaaaacaaa ttaatgttg tacgaggcg tccagttac gaccaatta cgaattaaa ttagaagtc tacgaaaaa tttaagcga tcgaaaaat ctgaaaatt gccggatcc agcagagga gagtaccgg ggggttaag gaaaaaccg aaaacgtcc gcatttgcc gaagacaaa aagcatttg ggcccaatt cctaggaaa tgttttaat tgcagcctt ataggacac gtagcggcc caatgtccg aaaccgaag agggagaaa ggttcttgt tacggctgt gggaaactg gaccatgtc atagcaaat tgtcccgat agaaaaagg aaagagagg cccattgag cgaagcgaa acggcaagg aatcacgca atgcttgtc gagaagtcg gggagagac tgcgaagtt gcggtggac cttgaatta ggtgatatt gctaaaaat cttttaggt gtagttgat tcaggtagt cctatatct ttcataaaa tcattcctc ataccacac aatttgcgt aagccttat ttggacact cagtgttac accgggatt aattcttca aaattgata attttagct ctttacgaa ggaactgtg tttgtaaat agtattaag acgtctatc ctatttcat gtcgtacct gatgaaaca atgaagtat gatgtcgca ttgggaagg aattttttg ttcacttcg ggattgaaa ataagtttt gacgataat aatatgata attgaacaa aaacctaat gagcgacgc gagagtttt gataatgaa ttgctctta atcgacacc acagtgttt gaaggcaag gaattagat tgtgttata aatcctgaa ttacctcga gaaacttta caaaaggta gaaaattta gtttatgaa tataacctg cctaaagtc aatgatacc caaggcact ccgaccgat tttcaaacc aaaattatt ttaagaccg gatcatact ccttttaat ttttatcct aggcgtttg tctttttac gaaaaaact caattacaa aagatttta gatgattta caaagagaa ggcattatt aaggaaagc aactcacct tatgcttct ccaattgtt ttggtaccc aaaaaatca ggagaatta aggctgaaa aagagctga
- >TC032556 RA
aaacttatt attattatt attattaaa attgcgtca tctgaaatg ctaaaacta cagtatact caaaagttc cgcgacgca tggttgaag gatcctatt ttgaaagac tggctagtg gttgtagaa agcattgca gggaaagag gcaaagtgt aaattttgt tcaaaaatt gtcactagt agatatgcg gatttgaag gctcatgga gaatccaag aaacacaag acaatcgct gaaactgcc ataggaaag ccaaaaatt tgcgaccac gtggtgggt actgccgtg tggggaaat atggcgaaa ctgggagaa gtgggtgaa ttaagagac aacattagg aaggctctg gtcagttcg gtgcgtacg ctttttaat attctattc ggaacgggc caaagacta aatcgtaca aaaatttgt gactttgag ggttttggg tgggaggta gactcggat gaatgtcga cagaaaatt gaaaaccta aaaaggggg tacagtgtc gcggaactt acggcggtt tgcgtgctt ttgggtata agttacgat gggaccgca gaggacctc gcgacgcgg ctgtgcacc gcattaaca gactttagt gccctcagt gccgttgcg gaagtggag gatgacgaa gaggaagac atccatacc gtagctaat gatacaaaa gtggcgacg ggcaatcga cctgcttcg agacaacaa atcatgccg cagacccca gtgccgaat ttttcgata tcgtttaga gacgtggag gattcgata cgacccttt tcaggcaca gacccggcg tatccagtc gaaaagttc gtggctgat tttgaagat ttggcaatg ttgttggga tggggagat ttgcaatca tacgacctc aaaactcga ttagtgcga gagttcgcg gccaaaata aacagcgcg gagttgcat caacaatta cgggaaaga aaaatgaaa aaaagtgaa tctgcgtat gaatatttt cttttgatg aaggagttg gcttcacgg ggcaatatt gaagaagaa gcggtggtg cactacatc gtagaagga atcatagat gacagtgaa aacaaatta atgttgtac gaggcgtcc agctacgac caattacga attaaatta gaagtctac gaaaaatat aagcgatcg aaaaatcta aaaattgcc ggatcctgc agaggagag taccagggg gttaaggaa aaaccgaaa acgtccgca tttgccgaa gacaaaaag catttgggc ccaattcca aggaaatgt tttaattgc agccttata ggacacgta gcggcccaa tgtccgaaa ccgaagagg gagaaaggt tcttgttac ggctgtggg aaactggac catgtcgta gcaaattgt cccgataga aaaaggaaa gagaggccc attgagcga agcgaaacg gcaaggaat cacgcaatg cttgttgag aagtcgggg agagactgc gaagttgcg gtggacctt gaattaggt gatattgct aaaaatctt ttaggtgta gttgattca gcggctcct aaagcgaat ggacaaata gaaagagtt aatcgcgta ttaacaccc atgcttgcc aaactgacg gtagagttg aataaatgg gacaaaagt ttaccgcaa ctcgaacta gttctaaat aatgcagca aatcgttcc acggggtat tccccatca aaattacta tttggggtc aatcaaagc tacttggag gatgataat attaaaacg tatttagag tctatacaa aaggatagt cgtgattta cttacaatt agggccgaa gcaagtacg aaaatggaa ttggtcggt aaatataat aaagaccaa tttgacaac cgtcataag agtcctaga aaataccag aaaagttgt gaccacgtg gtgggtact gccgtgtgg ggaaatatg gcgaaactg ggagaagtg ggtgaatta ggagacaac attaggaag gctccggtc agttcggcg cgtacgctt tttaatatt ctattcgga acgggccaa agactaaac cgtacaaaa atttgtgac tttgagggt ttcgtgtgg gaggtagac tcggatgaa tgtcaacag aaaattgaa aacataaaa agggggtac agtgtcgcg gaacttacg gcggtttgc gtgcttttg ggtataagt tacgatggg acagcagag gacctcgcg acgcggctg tgcatcgca ttaacagac tttagtgcc ctcaatgcc gttgcggaa gtggaggat gacgaagag gaagacatc cataccgta gctaatgat acagaagtg gcgacgggc aatcgacct gcttcgaga caacaaatc atgccgcag accccagtg ccgaatttt tcgatatcg tttagagac gtggaggat tcgatacga cccttttca ggcacagac ccggcgtat ccagtcgaa aagttcgtg gctgatttt gaagatttg gcaatgttg ttgggatgg ggagatttg caatcattt ttatttgca aaaaagtcg ttaatcggg ttagccaaa atgtttgtc cagggttta cacggagta aggacgtgg tacgacctc aaaactcga ttagtgcga gagttcgcg gccaaaata aacagcgcg gagttgcat caacaatta cgggaaaga aaaatgaaa aaaagtgaa tctgcgtat gaatatttt cttttgatg aaggagttg gcttcacgg ggcaatatt gaagaagaa gcggtggtg cactacatc gtagaagga atcttagat gacagtgaa aacaaatta atgttgtac gaggcgtcc agttacgac caattacga attaaatta gaagtctac gaaaaattt aagcgatcg aaaaatctg aaaattgcc ggatccagc agaggagag taccggggg gttaaggaa aaaccgaaa acgtccgca tttgccgaa gacaaaaag catttgggc ccaattcct aggaaatgt tttaattgc agccttata ggacacgta gcggcccaa tgtccgaaa ccgaagagg gagaaaggt tcttgttac ggctgtggg aaactggac catgtcata gcaaattgt cccgataga aaaaggaaa gagaggccc attgagcga agcgaaacg gcaaggaat cacgcaatg cttgtcgag aagtcgggg agagactgc gaagttgcg gtggacctt gaattaggt gatattgct aaaaatctt ttaggtgta gttgattca ggtagtcct atatctttc ataaaatca ttcctcata ccacacaat ttgcgtaag ccttatttg gacactcag tgttacacc gggattaat tcttcaaaa ttgataatt ttagctctt tacgaagga actgtgttt gtaaatagt attaagacg tctatccta tttcatgtc gtacctgat gaaacaatg aagtatgat gtcgcattg ggaaggaat tttttgttc acttcggga ttgaaaata agttttgac gataataat atgataatt gaacaaaaa cctaatgag cgacgcgag agttttgat aatgaattg ctcttaatc gacaccaca gtgtttgaa ggcaaggaa ttagattgt gttataaat cctgaatta cctcgagaa actttacaa aaggtagaa aatttagtt tatgaatat aacctgcct aaagtcaat gatacccaa ggcactccg accgatttt caaaccaaa attatttta agaccggat catactcct tttaatttt tatcctagg cgtttgtct ttttacgaa aaaactcaa ttacaaaag attttagat gatttacaa agagaaggc attattaag gaaagcaac tcaccttat gcttctcca attgttttg gtacccaaa aaatcagga gaattaagg ctgaaaaag agctgacag cttttgaat tattaaaaa atttacttg tatctcacc ccatacttg ccatttatt cacctaacg cagagacag agcttcatt gtgacgcat cagctgaag gatttggtg ccgttttac tacaaaaac aaccggata ataaattcc atccgattt catttttta gcaaaagga ccacgggaa atgagagta aatatcaca gctatgaat tagagtgtt tggcgatta tatatgccc tgaaacggt atcatcctt atttgcaag gtatcaaat ttaaaattg ttacagatt gtaatagta tcaccttaa ccttacata aaaaggagt tgaatccca gaatagcta gatgggcat tgtttttac aaaattatg attacatgt tagagcaca gaaatggca atcaaatga aacacgtag atgctctta gtcgaaatc atgtgctta ttttggaag ataatacat ttgagcaaa atttgtctt tcaaacaaa atcaagatt cttctttag ctgaaattc gtaatttac tagaaaatc aggaacatt caaaattcg aattaagaa acgggctaa tctattata agtttaaga ataagtcgt tattttata tacccgcaa aaatggaac agcaagtaa ttagaaaat atcatgatg attttggac acttaggtg tagataaag tagtagaat tgattacaa ggtattatt ggtttccta aattacggt ctaaagtta gtgaatata tatcaaatt gtctgaagt gcatatctt tttctccta actcaggta aacaggaag gtgagttac ataata
