Status
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Gene information
General information
TC032556
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016329,TC016328,TC016327
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20453254..20465297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032556
    iBeetle screen iB_06002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032556 RA
      MLKLQYTQK FRDAWLKDP ILKDWLVVV ESIAGKEAK 
      CKFCSKIVT SRYADLKAH GESKKHKTI AETAIGKPK 
      ICDHVVGTA VWGNMAKLG EVGELRDNI RKALVSSVR 
      TLFNILFGT GQRLNRTKI CDFEGFGWE VDSDECRQK 
      IENLKRGYS VAELTAVCV LLGISYDGT AEDLATRLC 
      TALTDFSAL SAVAEVEDD EEEDIHTVA NDTKVATGN 
      RPASRQQIM PQTPVPNFS ISFRDVEDS IRPFSGTDP 
      AYPVEKFVA DFEDLAMLL GWGDLQSYD LKTRLVREF 
      AAKINSAEL HQQLRERKM KKSESAYEY FLLMKELAS 
      RGNIEEEAV VHYIVEGII DDSENKLML YEASSYDQL 
      RIKLEVYEK YKRSKNLKI AGSCRGEYQ GVKEKPKTS 
      AFAEDKKHL GPIPRKCFN CSLIGHVAA QCPKPKREK 
      GSCYGCGKL DHVVANCPD RKRKERPIE RSETARNHA 
      MLVEKSGRD CEVAVDLEL GDIAKNLLG VVDSAAPKA 
      NGQIERVNR VLTPMLAKL TVELNKWDK SLPQLELVL 
      NNAANRSTG YSPSKLLFG VNQSYLEDD NIKTYLESI 
      QKDSRDLLT IRAEASTKM ELVGKYNKD QFDNRHKSP 
      RKYQKSCDH VVGTAVWGN MAKLGEVGE LGDNIRKAP 
      VSSARTLFN ILFGTGQRL NRTKICDFE GFVWEVDSD 
      ECQQKIENI KRGYSVAEL TAVCVLLGI SYDGTAEDL 
      ATRLCIALT DFSALNAVA EVEDDEEED IHTVANDTE 
      VATGNRPAS RQQIMPQTP VPNFSISFR DVEDSIRPF 
      SGTDPAYPV EKFVADFED LAMLLGWGD LQSFLFAKK 
      SLIGLAKMF VQGLHGVRT WYDLKTRLV REFAAKINS 
      AELHQQLRE RKMKKSESA YEYFLLMKE LASRGNIEE 
      EAVVHYIVE GILDDSENK LMLYEASSY DQLRIKLEV 
      YEKFKRSKN LKIAGSSRG EYRGVKEKP KTSAFAEDK 
      KHLGPIPRK CFNCSLIGH VAAQCPKPK REKGSCYGC 
      GKLDHVIAN CPDRKRKER PIERSETAR NHAMLVEKS 
      GRDCEVAVD LELGDIAKN LLGVVDSGS PISFIKSFL 
      IPHNLRKPY LDTQCYTGI NSSKLIILA LYEGTVFVN 
      SIKTSILFH VVPDETMKY DVALGRNFL FTSGLKISF 
      DDNNMIIEQ KPNERRESF DNELLLIDT TVFEGKELD 
      CVINPELPR ETLQKVENL VYEYNLPKV NDTQGTPTD 
      FQTKIILRP DHTPFNFYP RRLSFYEKT QLQKILDDL 
      QREGIIKES NSPYASPIV LVPKKSGEL RLKKS
    CDS sequence
    • >TC032556 RA
      atgctaaaa ctacagtat actcaaaag ttccgcgac 
      gcatggttg aaggatcct attttgaaa gactggcta 
      gtggttgta gaaagcatt gcagggaaa gaggcaaag 
      tgtaaattt tgttcaaaa attgtcact agtagatat 
      gcggatttg aaggctcat ggagaatcc aagaaacac 
      aagacaatc gctgaaact gccatagga aagccaaaa 
      atttgcgac cacgtggtg ggtactgcc gtgtgggga 
      aatatggcg aaactggga gaagtgggt gaattaaga 
      gacaacatt aggaaggct ctggtcagt tcggtgcgt 
      acgcttttt aatattcta ttcggaacg ggccaaaga 
      ctaaatcgt acaaaaatt tgtgacttt gagggtttt 
      gggtgggag gtagactcg gatgaatgt cgacagaaa 
      attgaaaac ctaaaaagg gggtacagt gtcgcggaa 
      cttacggcg gtttgcgtg cttttgggt ataagttac 
      gatgggacc gcagaggac ctcgcgacg cggctgtgc 
      accgcatta acagacttt agtgccctc agtgccgtt 
      gcggaagtg gaggatgac gaagaggaa gacatccat 
      accgtagct aatgataca aaagtggcg acgggcaat 
      cgacctgct tcgagacaa caaatcatg ccgcagacc 
      ccagtgccg aatttttcg atatcgttt agagacgtg 
      gaggattcg atacgaccc ttttcaggc acagacccg 
      gcgtatcca gtcgaaaag ttcgtggct gattttgaa 
      gatttggca atgttgttg ggatgggga gatttgcaa 
      tcatacgac ctcaaaact cgattagtg cgagagttc 
      gcggccaaa ataaacagc gcggagttg catcaacaa 
      ttacgggaa agaaaaatg aaaaaaagt gaatctgcg 
      tatgaatat tttcttttg atgaaggag ttggcttca 
      cggggcaat attgaagaa gaagcggtg gtgcactac 
      atcgtagaa ggaatcata gatgacagt gaaaacaaa 
      ttaatgttg tacgaggcg tccagctac gaccaatta 
      cgaattaaa ttagaagtc tacgaaaaa tataagcga 
      tcgaaaaat ctaaaaatt gccggatcc tgcagagga 
      gagtaccag ggggttaag gaaaaaccg aaaacgtcc 
      gcatttgcc gaagacaaa aagcatttg ggcccaatt 
      ccaaggaaa tgttttaat tgcagcctt ataggacac 
      gtagcggcc caatgtccg aaaccgaag agggagaaa 
      ggttcttgt tacggctgt gggaaactg gaccatgtc 
      gtagcaaat tgtcccgat agaaaaagg aaagagagg 
      cccattgag cgaagcgaa acggcaagg aatcacgca 
      atgcttgtt gagaagtcg gggagagac tgcgaagtt 
      gcggtggac cttgaatta ggtgatatt gctaaaaat 
      cttttaggt gtagttgat tcagcggct cctaaagcg 
      aatggacaa atagaaaga gttaatcgc gtattaaca 
      cccatgctt gccaaactg acggtagag ttgaataaa 
      tgggacaaa agtttaccg caactcgaa ctagttcta 
      aataatgca gcaaatcgt tccacgggg tattcccca 
      tcaaaatta ctatttggg gtcaatcaa agctacttg 
      gaggatgat aatattaaa acgtattta gagtctata 
      caaaaggat agtcgtgat ttacttaca attagggcc 
      gaagcaagt acgaaaatg gaattggtc ggtaaatat 
      aataaagac caatttgac aaccgtcat aagagtcct 
      agaaaatac cagaaaagt tgtgaccac gtggtgggt 
      actgccgtg tggggaaat atggcgaaa ctgggagaa 
      gtgggtgaa ttaggagac aacattagg aaggctccg 
      gtcagttcg gcgcgtacg ctttttaat attctattc 
      ggaacgggc caaagacta aaccgtaca aaaatttgt 
      gactttgag ggtttcgtg tgggaggta gactcggat 
      gaatgtcaa cagaaaatt gaaaacata aaaaggggg 
      tacagtgtc gcggaactt acggcggtt tgcgtgctt 
      ttgggtata agttacgat gggacagca gaggacctc 
      gcgacgcgg ctgtgcatc gcattaaca gactttagt 
      gccctcaat gccgttgcg gaagtggag gatgacgaa 
      gaggaagac atccatacc gtagctaat gatacagaa 
      gtggcgacg ggcaatcga cctgcttcg agacaacaa 
      atcatgccg cagacccca gtgccgaat ttttcgata 
      tcgtttaga gacgtggag gattcgata cgacccttt 
      tcaggcaca gacccggcg tatccagtc gaaaagttc 
      gtggctgat tttgaagat ttggcaatg ttgttggga 
      tggggagat ttgcaatca tttttattt gcaaaaaag 
      tcgttaatc gggttagcc aaaatgttt gtccagggt 
      ttacacgga gtaaggacg tggtacgac ctcaaaact 
      cgattagtg cgagagttc gcggccaaa ataaacagc 
      gcggagttg catcaacaa ttacgggaa agaaaaatg 
      aaaaaaagt gaatctgcg tatgaatat tttcttttg 
      atgaaggag ttggcttca cggggcaat attgaagaa 
      gaagcggtg gtgcactac atcgtagaa ggaatctta 
      gatgacagt gaaaacaaa ttaatgttg tacgaggcg 
      tccagttac gaccaatta cgaattaaa ttagaagtc 
      tacgaaaaa tttaagcga tcgaaaaat ctgaaaatt 
      gccggatcc agcagagga gagtaccgg ggggttaag 
      gaaaaaccg aaaacgtcc gcatttgcc gaagacaaa 
      aagcatttg ggcccaatt cctaggaaa tgttttaat 
      tgcagcctt ataggacac gtagcggcc caatgtccg 
      aaaccgaag agggagaaa ggttcttgt tacggctgt 
      gggaaactg gaccatgtc atagcaaat tgtcccgat 
      agaaaaagg aaagagagg cccattgag cgaagcgaa 
      acggcaagg aatcacgca atgcttgtc gagaagtcg 
      gggagagac tgcgaagtt gcggtggac cttgaatta 
      ggtgatatt gctaaaaat cttttaggt gtagttgat 
      tcaggtagt cctatatct ttcataaaa tcattcctc 
      ataccacac aatttgcgt aagccttat ttggacact 
      cagtgttac accgggatt aattcttca aaattgata 
      attttagct ctttacgaa ggaactgtg tttgtaaat 
      agtattaag acgtctatc ctatttcat gtcgtacct 
      gatgaaaca atgaagtat gatgtcgca ttgggaagg 
      aattttttg ttcacttcg ggattgaaa ataagtttt 
      gacgataat aatatgata attgaacaa aaacctaat 
      gagcgacgc gagagtttt gataatgaa ttgctctta 
      atcgacacc acagtgttt gaaggcaag gaattagat 
      tgtgttata aatcctgaa ttacctcga gaaacttta 
      caaaaggta gaaaattta gtttatgaa tataacctg 
      cctaaagtc aatgatacc caaggcact ccgaccgat 
      tttcaaacc aaaattatt ttaagaccg gatcatact 
      ccttttaat ttttatcct aggcgtttg tctttttac 
      gaaaaaact caattacaa aagatttta gatgattta 
      caaagagaa ggcattatt aaggaaagc aactcacct 
      tatgcttct ccaattgtt ttggtaccc aaaaaatca 
      ggagaatta aggctgaaa aagagctga
    mRNA sequence
    • >TC032556 RA
      aaacttatt attattatt attattaaa attgcgtca 
      tctgaaatg ctaaaacta cagtatact caaaagttc 
      cgcgacgca tggttgaag gatcctatt ttgaaagac 
      tggctagtg gttgtagaa agcattgca gggaaagag 
      gcaaagtgt aaattttgt tcaaaaatt gtcactagt 
      agatatgcg gatttgaag gctcatgga gaatccaag 
      aaacacaag acaatcgct gaaactgcc ataggaaag 
      ccaaaaatt tgcgaccac gtggtgggt actgccgtg 
      tggggaaat atggcgaaa ctgggagaa gtgggtgaa 
      ttaagagac aacattagg aaggctctg gtcagttcg 
      gtgcgtacg ctttttaat attctattc ggaacgggc 
      caaagacta aatcgtaca aaaatttgt gactttgag 
      ggttttggg tgggaggta gactcggat gaatgtcga 
      cagaaaatt gaaaaccta aaaaggggg tacagtgtc 
      gcggaactt acggcggtt tgcgtgctt ttgggtata 
      agttacgat gggaccgca gaggacctc gcgacgcgg 
      ctgtgcacc gcattaaca gactttagt gccctcagt 
      gccgttgcg gaagtggag gatgacgaa gaggaagac 
      atccatacc gtagctaat gatacaaaa gtggcgacg 
      ggcaatcga cctgcttcg agacaacaa atcatgccg 
      cagacccca gtgccgaat ttttcgata tcgtttaga 
      gacgtggag gattcgata cgacccttt tcaggcaca 
      gacccggcg tatccagtc gaaaagttc gtggctgat 
      tttgaagat ttggcaatg ttgttggga tggggagat 
      ttgcaatca tacgacctc aaaactcga ttagtgcga 
      gagttcgcg gccaaaata aacagcgcg gagttgcat 
      caacaatta cgggaaaga aaaatgaaa aaaagtgaa 
      tctgcgtat gaatatttt cttttgatg aaggagttg 
      gcttcacgg ggcaatatt gaagaagaa gcggtggtg 
      cactacatc gtagaagga atcatagat gacagtgaa 
      aacaaatta atgttgtac gaggcgtcc agctacgac 
      caattacga attaaatta gaagtctac gaaaaatat 
      aagcgatcg aaaaatcta aaaattgcc ggatcctgc 
      agaggagag taccagggg gttaaggaa aaaccgaaa 
      acgtccgca tttgccgaa gacaaaaag catttgggc 
      ccaattcca aggaaatgt tttaattgc agccttata 
      ggacacgta gcggcccaa tgtccgaaa ccgaagagg 
      gagaaaggt tcttgttac ggctgtggg aaactggac 
      catgtcgta gcaaattgt cccgataga aaaaggaaa 
      gagaggccc attgagcga agcgaaacg gcaaggaat 
      cacgcaatg cttgttgag aagtcgggg agagactgc 
      gaagttgcg gtggacctt gaattaggt gatattgct 
      aaaaatctt ttaggtgta gttgattca gcggctcct 
      aaagcgaat ggacaaata gaaagagtt aatcgcgta 
      ttaacaccc atgcttgcc aaactgacg gtagagttg 
      aataaatgg gacaaaagt ttaccgcaa ctcgaacta 
      gttctaaat aatgcagca aatcgttcc acggggtat 
      tccccatca aaattacta tttggggtc aatcaaagc 
      tacttggag gatgataat attaaaacg tatttagag 
      tctatacaa aaggatagt cgtgattta cttacaatt 
      agggccgaa gcaagtacg aaaatggaa ttggtcggt 
      aaatataat aaagaccaa tttgacaac cgtcataag 
      agtcctaga aaataccag aaaagttgt gaccacgtg 
      gtgggtact gccgtgtgg ggaaatatg gcgaaactg 
      ggagaagtg ggtgaatta ggagacaac attaggaag 
      gctccggtc agttcggcg cgtacgctt tttaatatt 
      ctattcgga acgggccaa agactaaac cgtacaaaa 
      atttgtgac tttgagggt ttcgtgtgg gaggtagac 
      tcggatgaa tgtcaacag aaaattgaa aacataaaa 
      agggggtac agtgtcgcg gaacttacg gcggtttgc 
      gtgcttttg ggtataagt tacgatggg acagcagag 
      gacctcgcg acgcggctg tgcatcgca ttaacagac 
      tttagtgcc ctcaatgcc gttgcggaa gtggaggat 
      gacgaagag gaagacatc cataccgta gctaatgat 
      acagaagtg gcgacgggc aatcgacct gcttcgaga 
      caacaaatc atgccgcag accccagtg ccgaatttt 
      tcgatatcg tttagagac gtggaggat tcgatacga 
      cccttttca ggcacagac ccggcgtat ccagtcgaa 
      aagttcgtg gctgatttt gaagatttg gcaatgttg 
      ttgggatgg ggagatttg caatcattt ttatttgca 
      aaaaagtcg ttaatcggg ttagccaaa atgtttgtc 
      cagggttta cacggagta aggacgtgg tacgacctc 
      aaaactcga ttagtgcga gagttcgcg gccaaaata 
      aacagcgcg gagttgcat caacaatta cgggaaaga 
      aaaatgaaa aaaagtgaa tctgcgtat gaatatttt 
      cttttgatg aaggagttg gcttcacgg ggcaatatt 
      gaagaagaa gcggtggtg cactacatc gtagaagga 
      atcttagat gacagtgaa aacaaatta atgttgtac 
      gaggcgtcc agttacgac caattacga attaaatta 
      gaagtctac gaaaaattt aagcgatcg aaaaatctg 
      aaaattgcc ggatccagc agaggagag taccggggg 
      gttaaggaa aaaccgaaa acgtccgca tttgccgaa 
      gacaaaaag catttgggc ccaattcct aggaaatgt 
      tttaattgc agccttata ggacacgta gcggcccaa 
      tgtccgaaa ccgaagagg gagaaaggt tcttgttac 
      ggctgtggg aaactggac catgtcata gcaaattgt 
      cccgataga aaaaggaaa gagaggccc attgagcga 
      agcgaaacg gcaaggaat cacgcaatg cttgtcgag 
      aagtcgggg agagactgc gaagttgcg gtggacctt 
      gaattaggt gatattgct aaaaatctt ttaggtgta 
      gttgattca ggtagtcct atatctttc ataaaatca 
      ttcctcata ccacacaat ttgcgtaag ccttatttg 
      gacactcag tgttacacc gggattaat tcttcaaaa 
      ttgataatt ttagctctt tacgaagga actgtgttt 
      gtaaatagt attaagacg tctatccta tttcatgtc 
      gtacctgat gaaacaatg aagtatgat gtcgcattg 
      ggaaggaat tttttgttc acttcggga ttgaaaata 
      agttttgac gataataat atgataatt gaacaaaaa 
      cctaatgag cgacgcgag agttttgat aatgaattg 
      ctcttaatc gacaccaca gtgtttgaa ggcaaggaa 
      ttagattgt gttataaat cctgaatta cctcgagaa 
      actttacaa aaggtagaa aatttagtt tatgaatat 
      aacctgcct aaagtcaat gatacccaa ggcactccg 
      accgatttt caaaccaaa attatttta agaccggat 
      catactcct tttaatttt tatcctagg cgtttgtct 
      ttttacgaa aaaactcaa ttacaaaag attttagat 
      gatttacaa agagaaggc attattaag gaaagcaac 
      tcaccttat gcttctcca attgttttg gtacccaaa 
      aaatcagga gaattaagg ctgaaaaag agctgacag 
      cttttgaat tattaaaaa atttacttg tatctcacc 
      ccatacttg ccatttatt cacctaacg cagagacag 
      agcttcatt gtgacgcat cagctgaag gatttggtg 
      ccgttttac tacaaaaac aaccggata ataaattcc 
      atccgattt catttttta gcaaaagga ccacgggaa 
      atgagagta aatatcaca gctatgaat tagagtgtt 
      tggcgatta tatatgccc tgaaacggt atcatcctt 
      atttgcaag gtatcaaat ttaaaattg ttacagatt 
      gtaatagta tcaccttaa ccttacata aaaaggagt 
      tgaatccca gaatagcta gatgggcat tgtttttac 
      aaaattatg attacatgt tagagcaca gaaatggca 
      atcaaatga aacacgtag atgctctta gtcgaaatc 
      atgtgctta ttttggaag ataatacat ttgagcaaa 
      atttgtctt tcaaacaaa atcaagatt cttctttag 
      ctgaaattc gtaatttac tagaaaatc aggaacatt 
      caaaattcg aattaagaa acgggctaa tctattata 
      agtttaaga ataagtcgt tattttata tacccgcaa 
      aaatggaac agcaagtaa ttagaaaat atcatgatg 
      attttggac acttaggtg tagataaag tagtagaat 
      tgattacaa ggtattatt ggtttccta aattacggt 
      ctaaagtta gtgaatata tatcaaatt gtctgaagt 
      gcatatctt tttctccta actcaggta aacaggaag 
      gtgagttac ataata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY