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Gene information
General information
TC016321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 888273..891221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016321
    iBeetle screen iB_06000

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 55.56% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016321 RA
      MWNFQISVF FTLLSILDS SKADTNCTE LKEQFASAT 
      AEFSSCAIQ NSRPVTFCE NCVNAYIQI VDSYNGMKE 
      VPDNGTYCI DRFVNLDRL GIVQTSYIN SYNLWNNAK 
      CYECFQVVD GKLTPNKSL ETIAFNKYY DNFMQCING 
      TDINDTTLC PTCIDDYLD LNSYYHSIS NENEKIGGC 
      MDMVDLINN TRQFWSQKC CKYRRHDEH IFLASAITV 
      LIVTLLFYV IAQFSSVKK TPTIMQQTR FAESLSHNS 
      QS
    CDS sequence
    • >TC016321 RA
      atgtggaat tttcaaatt tccgtgttt tttacgctt 
      ctaagcatc ctggactca tcaaaagca gacacaaac 
      tgtactgaa ctcaaagaa cagtttgca agtgctaca 
      gcagagttc agttcttgt gcaatccaa aattccaga 
      ccagtaaca ttctgtgaa aattgtgtt aacgcttat 
      atacaaata gtggatagt tacaacggt atgaaagag 
      gttccagac aatggaacg tactgcatt gatagattc 
      gtaaattta gataggtta ggaattgtc caaacttcg 
      tacatcaac agttacaat ttgtggaat aacgccaaa 
      tgttacgaa tgtttccaa gttgttgat ggaaaatta 
      acaccaaat aaatcactt gaaaccata gctttcaac 
      aaatattat gataatttt atgcagtgt atcaatgga 
      actgatatt aatgatacg acattgtgc ccgacttgt 
      atagacgat tatttagat ttaaatagt tactatcac 
      agcatcagt aatgaaaac gagaaaatt gggggatgc 
      atggatatg gtcgattta ataaataat accagacaa 
      ttctggagt caaaaatgc tgcaaatat cgcagacac 
      gatgaacac attttttta gcatcggca attacagtt 
      cttatagta actttactc ttttacgta attgcacaa 
      ttttcttca gtgaagaag acaccaaca ataatgcaa 
      cagacccga tttgcagaa tcgttaagc cacaacagc 
      cagtcataa
    mRNA sequence
    • >TC016321 RA
      agttcgtaa tttaaacaa aactaataa atacgggga 
      tttttttat gttatcacg tacatatcg tatcagttt 
      agtaatatg tgagcaaca gtcgtagta caaatgtgg 
      aattttcaa atttccgtg ttttttacg cttctaagc 
      atcctggac tcatcaaaa gcagacaca aactgtact 
      gaactcaaa gaacagttt gcaagtgct acagcagag 
      ttcagttct tgtgcaatc caaaattcc agaccagta 
      acattctgt gaaaattgt gttaacgct tatatacaa 
      atagtggat agttacaac ggtatgaaa gaggttcca 
      gacaatgga acgtactgc attgataga ttcgtaaat 
      ttagatagg ttaggaatt gtccaaact tcgtacatc 
      aacagttac aatttgtgg aataacgcc aaatgttac 
      gaatgtttc caagttgtt gatggaaaa ttaacacca 
      aataaatca cttgaaacc atagctttc aacaaatat 
      tatgataat tttatgcag tgtatcaat ggaactgat 
      attaatgat acgacattg tgcccgact tgtatagac 
      gattattta gatttaaat agttactat cacagcatc 
      agtaatgaa aacgagaaa attggggga tgcatggat 
      atggtcgat ttaataaat aataccaga caattctgg 
      agtcaaaaa tgctgcaaa tatcgcaga cacgatgaa 
      cacattttt ttagcatcg gcaattaca gttcttata 
      gtaacttta ctcttttac gtaattgca caattttct 
      tcagtgaag aagacacca acaataatg caacagacc 
      cgatttgca gaatcgtta agccacaac agccagtca 
      taaaacaat gtgatgttt tttattaaa atatttaca 
      ataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY