Status
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Gene information
General information
TC016301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9708108..9710764
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC016301
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05993

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016301 RA
        MSNKKTLNI VVIGETGVG KSTFINMFS TYFRHDDID 
        AAVAEYPDN VLIPTLVTI YDENGQDLE FKVGSNKDQ 
        IQEAGCSGT QTVNSYLFK CEGSDFNIN LVDTPGMGD 
        TNCVSKDNE HCEMILNEL SELGEIHAI CFLMRPTNT 
        RNTFFFDYC IKEIMSRLH KSACHNMIF VFTNTRSQN 
        YEPGDTLPG LKRMITAIN ERLTDVKIK LHKNTNIFC 
        FDNESIKYL VAKHNNVNL NTKVVEDYK QSWDVSKKE 
        FSRLIEYVN TLEPHDARN SVSLNKTRR LISEILKPL 
        SDISDLLQD NIQNLIKNE EEISKVRET QKDLEDKLY 
        ITETELEII QLETPANIC TNSSCATQE NQSEGVQKW 
        HYKEKCHEC PWVGNLIKE ESIGHWGLW FCSQIAFNG 
        TCKKCNCSA SHHMIIFYE TKKHQKSVL VPVVQRELE 
        STESKLDDF WQYMQQVSQ RKDELQNEL DFAAQCAAK 
        FAIFIKKCA VVPINDAYE GYISLLIKR EEYQEESDN 
        LKLEVLRHM LQNHRELKT KFFETSNSL PTYFSVSSQ 
        SIEEDIKKL YSCKHIGPK LKEISEKRN KCIVIAEET 
        GSNKKKHSI RIFLGGLTF KLKNKVVSS LKSLGVYGQ 
        YNGPNEAAE PATEALEAG DFH
      CDS sequence
      • >TC016301 RA
        atgtctaat aaaaaaacc ttaaatatc gttgttatc 
        ggagagacg ggagtcggg aaatcaacg tttatcaac 
        atgttctcc acatatttt agacatgac gatatcgat 
        gctgcagtt gcagaatat ccagataat gttcttata 
        cctacgttg gtgacaatt tacgatgaa aatggtcaa 
        gatttagaa tttaaagtg ggttcaaac aaagatcaa 
        attcaggaa gcaggttgc agtggcact caaactgtt 
        aattcgtac ttgttcaaa tgtgaggga agtgacttt 
        aatataaat ttagtggac actccaggg atgggagat 
        acaaactgc gtatctaaa gacaatgaa cactgtgaa 
        atgattttg aatgaattg tctgaatta ggtgaaatt 
        catgccatt tgctttttg atgagacca acgaacacc 
        agaaatacc tttttcttt gactactgt attaaagaa 
        ataatgtca cgcttacat aaatcagcg tgccacaac 
        atgattttc gtttttaca aacacgagg agtcaaaat 
        tatgaacca ggagatacc ttacctgga ttgaaaaga 
        atgataaca gcgattaat gaaagatta acagatgta 
        aagatcaaa ctgcataaa aatacaaat atcttttgt 
        tttgacaat gaaagtatt aagtattta gtggcaaaa 
        cacaataat gtgaactta aataccaaa gtagtggag 
        gactataag caaagttgg gatgtttcc aagaaagaa 
        ttttcacgg ctcattgaa tacgttaat accctggaa 
        ccacatgat gcaagaaac agtgtaagt ctaaataaa 
        acaagaagg ttaatttcc gaaattctt aaacctcta 
        tcagacatc tctgatctt ttacaagac aatatacaa 
        aatttaatt aagaacgaa gaagagata agcaaagta 
        agagaaacg caaaaagat ttagaagat aaattatac 
        atcactgaa accgaattg gaaataatt cagttggaa 
        acaccagcc aatatttgt accaattca agctgtgcc 
        acgcaggaa aatcagagt gaaggtgtg caaaaatgg 
        cattataag gaaaaatgt catgaatgc ccttgggtg 
        ggaaaccta ataaaagaa gagagtatt ggtcactgg 
        ggtctttgg ttttgttcc caaattgca tttaatggc 
        acgtgcaag aaatgcaac tgttcagct tcacatcat 
        atgataatt ttttatgaa accaaaaaa catcaaaaa 
        agtgtgttg gtacctgtt gttcaaaga gaacttgaa 
        tccacagag agtaaactc gatgatttt tggcaatat 
        atgcaacaa gtatcacag cgaaaagac gagttacaa 
        aatgagctc gattttgca gcacaatgt gccgcaaaa 
        tttgcaatt ttcataaaa aaatgtgcg gtggtacct 
        ataaatgat gcctacgaa ggatatatc agcctttta 
        attaaacga gaggaatat caggaagaa tctgacaac 
        cttaaactt gaagttctt cgccatatg ctacaaaat 
        catagagaa ttgaaaaca aaatttttt gagacatca 
        aattcactc ccaacatat ttcagtgtt agttcccaa 
        agtatcgaa gaagacatc aaaaaattg tacagttgt 
        aaacacatt ggacccaaa ctaaaagaa atctccgaa 
        aagaggaat aaatgtatt gtaattgca gaagaaact 
        ggcagcaat aaaaaaaaa cattctatt cgaattttt 
        ttgggggga cttacattt aaactaaaa aataaagta 
        gtaagttct ctgaaaagt ttaggagtt tatggacaa 
        tataatgga ccgaatgaa gcggccgaa ccagccact 
        gaggccttg gaggctgga gatttccac tga
      mRNA sequence
      • >TC016301 RA
        tgttatcac agattagag aggtgatct gtgctgtta 
        ttctgtgct tattagacc agcagaaac cattgaatt 
        tcctaaagc atttagtga ttgagggtg ttaagttgg 
        tacacacag tgcaaaata cttaagttg aaacaacga 
        tagcaaagt tgtaatcct ccaaaatgt ctaataaaa 
        aaaccttaa atatcgttg ttatcggag agacgggag 
        tcgggaaat caacgttta tcaacatgt tctccacat 
        attttagac atgacgata tcgatgctg cagttgcag 
        aatatccag ataatgttc ttataccta cgttggtga 
        caatttacg atgaaaatg gtcaagatt tagaattta 
        aagtgggtt caaacaaag atcaaattc aggaagcag 
        gttgcagtg gcactcaaa ctgttaatt cgtacttgt 
        tcaaatgtg agggaagtg actttaata taaatttag 
        tggacactc cagggatgg gagatacaa actgcgtat 
        ctaaagaca atgaacact gtgaaatga ttttgaatg 
        aattgtctg aattaggtg aaattcatg ccatttgct 
        ttttgatga gaccaacga acaccagaa atacctttt 
        tctttgact actgtatta aagaaataa tgtcacgct 
        tacataaat cagcgtgcc acaacatga ttttcgttt 
        ttacaaaca cgaggagtc aaaattatg aaccaggag 
        ataccttac ctggattga aaagaatga taacagcga 
        ttaatgaaa gattaacag atgtaaaga tcaaactgc 
        ataaaaata caaatatct tttgttttg acaatgaaa 
        gtattaagt atttagtgg caaaacaca ataatgtga 
        acttaaata ccaaagtag tggaggact ataagcaaa 
        gttgggatg tttccaaga aagaatttt cacggctca 
        ttgaatacg ttaataccc tggaaccac atgatgcaa 
        gaaacagtg taagtctaa ataaaacaa gaaggttaa 
        tttccgaaa ttcttaaac ctctatcag acatctctg 
        atcttttac aagacaata tacaaaatt taattaaga 
        acgaagaag agataagca aagtaagag aaacgcaaa 
        aagatttag aagataaat tatacatca ctgaaaccg 
        aattggaaa taattcagt tggaaacac cagccaata 
        tttgtacca attcaagct gtgccacgc aggaaaatc 
        agagtgaag gtgtgcaaa aatggcatt ataaggaaa 
        aatgtcatg aatgccctt gggtgggaa acctaataa 
        aagaagaga gtattggtc actggggtc tttggtttt 
        gttcccaaa ttgcattta atggcacgt gcaagaaat 
        gcaactgtt cagcttcac atcatatga taatttttt 
        atgaaacca aaaaacatc aaaaaagtg tgttggtac 
        ctgttgttc aaagagaac ttgaatcca cagagagta 
        aactcgatg atttttggc aatatatgc aacaagtat 
        cacagcgaa aagacgagt tacaaaatg agctcgatt 
        ttgcagcac aatgtgccg caaaatttg caattttca 
        taaaaaaat gtgcggtgg tacctataa atgatgcct 
        acgaaggat atatcagcc ttttaatta aacgagagg 
        aatatcagg aagaatctg acaacctta aacttgaag 
        ttcttcgcc atatgctac aaaatcata gagaattga 
        aaacaaaat tttttgaga catcaaatt cactcccaa 
        catatttca gtgttagtt cccaaagta tcgaagaag 
        acatcaaaa aattgtaca gttgtaaac acattggac 
        ccaaactaa aagaaatct ccgaaaaga ggaataaat 
        gtattgtaa ttgcagaag aaactggca gcaataaaa 
        aaaaacatt ctattcgaa tttttttgg ggggactta 
        catttaaac taaaaaata aagtagtaa gttctctga 
        aaagtttag gagtttatg gacaatata atggaccga 
        atgaagcgg ccgaaccag ccactgagg ccttggagg 
        ctggagatt tccactgaa gtttcactg tacagttct 
        aaatctact atttagtac tgtactatt ctaaatgtt 
        ttaaacatt tgcttgtgt ttaacgtaa ataatgttt 
        atttcttgt ttagaatca agttcattt tgtaataac 
        aatttatta aataaatat agcatgtgt aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY