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Gene information
General information
TC016297 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9745559..9752334
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016297
    iBeetle screen iB_05991

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016297 RA
      MWSAVVPAD VESPAPSAR SKHSATIVG ENIYLLGGR 
      NGNLPMKDF WRYNLITGK WHLLKSTGD RLPALQEHS 
      TVAYRNCLY VFGGEVGFS AGTETPLWC YDIKTNNWK 
      KIRNKKGVM TPKGRRGHT ALVHRDQML LYGGYQDLR 
      GSCSELWAF HFETESWHL LSTTPKNAN IESLPPPRH 
      KHSAVIHDD SMWVYGGMT DLQERGDLW KWDTHSKSW 
      TFIKTKINP GPLHSHAVC KLPSSMLLF GGERGGHPT 
      NELWKFSFA TETWEKIIT SGAKPQPRS ESVAFSVSD 
      FSVNESAVT EKAVRLRSR TCISADRTN RHSSYLPNN 
      RIAPYEKTY IFRPSHTNY TDGSDPTHT IEEKKSFLQ 
      EISKISQIS KINKCSYTV LTGCTTDST ESLLKQHAS 
      PHFDETEAI PSKNAMVKS KSAYVIKKK VQVESETIT 
      REPISVPNF SVLTLPTPV LTPVEAAKL VYLDTDDEN 
      EILNEKCEF DKPVLNPPK RGESYSSHL GYADNPLYQ 
      HMMKLSEKE NISSTSDYA SIETMNRLS SASNYSVKT 
      NTAPEEPKN LEKDGPFGF CNPNYLGPD IKALLDPSE 
      KKQISKLLT HPENKDSEE NLLELQEYK NAKVYFRAS 
      TRLPSESHR SKEAKRRAH SAGRADSHK VDKNEADFD 
      RIIDFVPLY VYVIGGKEH GQVTIFRRP ISMWKLNLF
    CDS sequence
    • >TC016297 RA
      atgtggagt gccgtagtg cctgccgat gttgaaagt 
      ccagctcct tcggcacgg tctaaacac agtgctacc 
      atagttggg gaaaatatt tacctctta ggcgggcga 
      aatggtaat ttacccatg aaagatttc tggagatat 
      aatctgata accggcaaa tggcactta ctgaaatcc 
      accggtgac agacttcca gcattgcaa gaacactca 
      actgttgcg tatagaaat tgtctttac gtgttcgga 
      ggcgaagtc ggcttttca gcgggaact gaaactcct 
      ctgtggtgc tatgacatc aaaaccaac aactggaag 
      aaaatcaga aacaaaaaa ggcgtcatg acgccaaaa 
      ggccgtcga gggcacaca gctttagtc catcgcgac 
      caaatgctt ctatatgga ggctatcag gacctacga 
      gggtcctgt agtgaattg tgggctttc cattttgaa 
      actgagtct tggcactta ttatcgacc acaccaaag 
      aatgccaac atcgagtcg cttccaccc ccgcgtcat 
      aaacactcg gctgttatt catgatgat tccatgtgg 
      gtgtatggc ggaatgact gacttacag gaaagagga 
      gatttgtgg aagtgggat acgcattcc aagtcttgg 
      acttttatt aaaactaag atcaatccg gggcccttg 
      cacagtcat gccgtctgt aagcttccg tcttcgatg 
      ctgctcttt ggaggggaa agaggtgga caccctaca 
      aatgaattg tggaaattc agttttgca accgaaact 
      tgggaaaaa atcatcaca tctggtgcc aagccgcaa 
      ccgcggtcc gaaagcgtt gccttctcc gtctcggat 
      ttctccgta aacgaatcc gccgtcacc gagaaagcc 
      gtccgtctc cgttcccgg acttgcatc tccgccgac 
      cgcaccaat cgccactcg tcctacctc cccaacaac 
      cggatcgcc ccctacgag aaaacctac atttttcgc 
      ccttctcac accaactac accgacggc agcgatccc 
      actcacacc atcgaagag aaaaagagc ttcttgcaa 
      gaaatctcc aaaatctcc caaatttcc aaaatcaac 
      aaatgcagc tacaccgta ttgaccggc tgcacgacg 
      gacagcacc gagagcctc ctcaaacag cacgcaagc 
      ccgcatttc gacgaaacc gaagcaatt ccttccaaa 
      aacgcaatg gtcaagtca aaatccgcg tacgtgatc 
      aagaagaaa gtccaagtg gagagcgag acgataacg 
      cgagagccg atttcagta ccaaatttc tcggttttg 
      actctgcca actccagtt ctgacacca gtcgaagcg 
      gcgaaattg gtgtatttg gacaccgat gatgaaaat 
      gagattcta aacgaaaag tgcgagttt gataaacct 
      gttctgaat ccccctaaa agaggcgaa agctacagc 
      tcgcacctg ggctacgcc gacaaccct ctctaccaa 
      cacatgatg aaactgagc gaaaaagag aacatttcg 
      tccacttcc gactatgcc agcattgag acaatgaac 
      cgactctcc tcagccagt aattacagc gtcaagact 
      aacacagca ccagaagag cccaaaaac ctggaaaaa 
      gacggccct tttggtttt tgcaacccc aattatttg 
      ggacccgac atcaaagcg ttactagat ccaagcgag 
      aagaagcag atttccaag ctattgacc caccctgaa 
      aacaaggat tccgaagag aatcttttg gagctgcag 
      gagtacaag aatgcgaaa gtgtatttt agggcttcg 
      acgcgactt ccatccgag tcccatcgc agcaaagag 
      gcgaaacga agggcacat agtgccgga agggccgac 
      agccacaag gttgataaa aacgaggcc gattttgac 
      cggattatt gattttgtt ccattgtat gtctatgtc 
      attggaggg aaagagcat gggcaagtt acgattttt 
      cggaggccg atctcgatg tggaaatta aacctgttc 
      taa
    mRNA sequence
    • >TC016297 RA
      attgtgtag agtagaaac gcatgcgtc tgtatttta 
      ttgcgacaa caaaaataa tataaattg tccaagcca 
      tgtgtcttg tgctctttc ttacaaaat gtaataaat 
      ccacagtaa agaaatgtc taaaaaaac acactagtg 
      aataaacca ctctgggtc caaggaatt gtagaagaa 
      atctagtat gtggagtgc cgtagtgcc tgccgatgt 
      tgaaagtcc agctccttc ggcacggtc taaacacag 
      tgctaccat agttgggga aaatattta cctcttagg 
      cgggcgaaa tggtaattt acccatgaa agatttctg 
      gagatataa tctgataac cggcaaatg gcacttact 
      gaaatccac cggtgacag acttccagc attgcaaga 
      acactcaac tgttgcgta tagaaattg tctttacgt 
      gttcggagg cgaagtcgg cttttcagc gggaactga 
      aactcctct gtggtgcta tgacatcaa aaccaacaa 
      ctggaagaa aatcagaaa caaaaaagg cgtcatgac 
      gccaaaagg ccgtcgagg gcacacagc tttagtcca 
      tcgcgacca aatgcttct atatggagg ctatcagga 
      cctacgagg gtcctgtag tgaattgtg ggctttcca 
      ttttgaaac tgagtcttg gcacttatt atcgaccac 
      accaaagaa tgccaacat cgagtcgct tccaccccc 
      gcgtcataa acactcggc tgttattca tgatgattc 
      catgtgggt gtatggcgg aatgactga cttacagga 
      aagaggaga tttgtggaa gtgggatac gcattccaa 
      gtcttggac ttttattaa aactaagat caatccggg 
      gcccttgca cagtcatgc cgtctgtaa gcttccgtc 
      ttcgatgct gctctttgg aggggaaag aggtggaca 
      ccctacaaa tgaattgtg gaaattcag ttttgcaac 
      cgaaacttg ggaaaaaat catcacatc tggtgccaa 
      gccgcaacc gcggtccga aagcgttgc cttctccgt 
      ctcggattt ctccgtaaa cgaatccgc cgtcaccga 
      gaaagccgt ccgtctccg ttcccggac ttgcatctc 
      cgccgaccg caccaatcg ccactcgtc ctacctccc 
      caacaaccg gatcgcccc ctacgagaa aacctacat 
      ttttcgccc ttctcacac caactacac cgacggcag 
      cgatcccac tcacaccat cgaagagaa aaagagctt 
      cttgcaaga aatctccaa aatctccca aatttccaa 
      aatcaacaa atgcagcta caccgtatt gaccggctg 
      cacgacgga cagcaccga gagcctcct caaacagca 
      cgcaagccc gcatttcga cgaaaccga agcaattcc 
      ttccaaaaa cgcaatggt caagtcaaa atccgcgta 
      cgtgatcaa gaagaaagt ccaagtgga gagcgagac 
      gataacgcg agagccgat ttcagtacc aaatttctc 
      ggttttgac tctgccaac tccagttct gacaccagt 
      cgaagcggc gaaattggt gtatttgga caccgatga 
      tgaaaatga gattctaaa cgaaaagtg cgagtttga 
      taaacctgt tctgaatcc ccctaaaag aggcgaaag 
      ctacagctc gcacctggg ctacgccga caaccctct 
      ctaccaaca catgatgaa actgagcga aaaagagaa 
      catttcgtc cacttccga ctatgccag cattgagac 
      aatgaaccg actctcctc agccagtaa ttacagcgt 
      caagactaa cacagcacc agaagagcc caaaaacct 
      ggaaaaaga cggcccttt tggtttttg caaccccaa 
      ttatttggg acccgacat caaagcgtt actagatcc 
      aagcgagaa gaagcagat ttccaagct attgaccca 
      ccctgaaaa caaggattc cgaagagaa tcttttgga 
      gctgcagga gtacaagaa tgcgaaagt gtattttag 
      ggcttcgac gcgacttcc atccgagtc ccatcgcag 
      caaagaggc gaaacgaag ggcacatag tgccggaag 
      ggccgacag ccacaaggt tgataaaaa cgaggccga 
      ttttgaccg gattattga ttttgttcc attgtatgt 
      ctatgtcat tggagggaa agagcatgg gcaagttac 
      gatttttcg gaggccgat ctcgatgtg gaaattaaa 
      cctgttcta atttttttg caatagacg tgccaaagt 
      tagagttcc aggagttag attttctac cttttctaa 
      tagtttagt ttagataga aataaaatg agaaattcg 
      tccaatacg ttgtgatca agtttaata agaagagtc 
      actattttc ggacgaaag ccttgtaga tttagtcga 
      aagtgcaat atattttca aatttcga
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