| General information | |||
| TC034370
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 9822243..10032571 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034370 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05990 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
ovary without detectable defects - Number of animals showing the phenotype: 1
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC034370 RA
MVMKDPATR RSRGFGFIT FTDPASVDK VLAQGTHEL DGKKIDPKV AFPRRAHPK MVTRTKKIF VGGLSAPTT LEDVKSYFE QFGPIEDAM LMFDKQTNR HRGFGFVTF QSEDVVDKV CEIHFHEIN NKMVECKKA QPKEVMLPA NLAKTRAAG RGAYDFMWS LGALPDGFP AAAYAAYAA GRGYSGYPS FGLPYPTVD LTNGQLTPN NNTPLLTQA LSVMNNYQA AAAAAQGFG PPASPHAAN TRGGFPAAN SPGPTIDMY NSSAGDSVG YVQAASPQP SSFPAIAVS RAPLNYSPK TGPAGGCMS LIRCGATAT VASSAVRDL GTFQHRLSY TNISKAGRG LARTFKEGH VEL
- >TC034370 RB
MVMKDPATR RSRGFGFIT FTDPASVDK VLAQGTHEL DGKKIDPKV AFPRRAHPK MVTRTKKIF VGGLSAPTT LEDVKSYFE QFGPIEDAM LMFDKQTNR HRGFGFVTF QSEDVVDKV CEIHFHEIN NKMVECKKA QPKEVMLPA NLAKTRAAG RGAYDFMWS LGALPDGFP AAAYAAYAA GRGYSGYPS FGLPYPTVD LTNGQLTPN NNTPLLTQA LSVMNNYQA AAAAAQGFG PPASPHAAN TRGGFPAAN SPGPTIDMY NSSAGDSVG YVQAASPQP SSFPAIAVS RAPLNYSPG PLIPAAFTN GYH
- >TC034370 RC
MVMKDPATR RSSTNCDGS AVFGVDSVT RLFTPRGFG FITFTDPAS VDKVLAQGT HELDGKKID PKVAFPRRA HPKMVTRTK KIFVGGLSA PTTLEDVKS YFEQFGPIE DAMLMFDKQ TNRHRGFGF VTFQSEDVV DKVCEIHFH EINNKMVEC KKAQPKEVM LPANLAKTR AAGRGAYDF MWSLGALPD GFPAAAYAA YAAGRGYSG YPSFGLPYP TVDLTNGQL TPNNNTPLL TQALSVMNN YQAAAAAAQ GFGPPASPH AANTRGGFP AANSPGPTI DMYNSSAGD SVGYVQAAS PQPSSFPAI AVSRAPLNY SPGPLIPAA FTNGYH
- >TC034370 RA
atggtaatg aaagatcct gccaccagg cggtcgagg ggctttgga tttatcacc ttcaccgac ccggctagc gtagataag gttttagcc caaggcact cacgaacta gatggcaaa aagattgac cccaaagta gcgttcccg agaagagca cacccaaag atggtaacc cggacgaaa aaaatattc gtcggtgga ttatcagcg cccactacc ctagaggac gtcaaaagt tacttcgaa caattcggt ccgattgaa gatgcaatg ctaatgttc gataagcaa acgaatcga cataggggt tttggattt gtcacattt caaagcgaa gatgttgta gataaagtt tgtgaaatc catttccat gagattaac aataaaatg gtggaatgt aagaaagcg caaccaaaa gaagtaatg ctccccgcc aacctagcg aagacacgg gcggcaggc aggggtgct tacgatttt atgtggtca cttggagct cttcctgac ggttttccg gcagccgca tacgcggcc tacgcggcc ggtcgaggt tactcgggc taccctagt ttcggacta ccgtaccca acagtggac ctaaccaac ggacaactg acacctaac aataacacc ccactattg actcaggcc ttgtcagtg atgaataac taccaggct gcggcggcg gcagcgcag ggcttcgga ccgccggct tcgccccat gcagccaac acacgtgga ggatttcct gctgccaac tcacctgga cctaccata gatatgtac aatagcagt gcgggggat agtgtgggc tatgtgcag gccgctagc ccacaaccc tccagcttc ccggcgatc gccgtcagc agggcccct cttaactac agtccgaag acaggaccg gccggtggc tgcatgtct ttgataaga tgtggggcc actgcgacg gtcgcgtcg agcgctgtc cgcgatttg ggcacattc cagcaccgc ctctcgtat accaacata tcaaaggcc ggtcgcggg ctcgctcgg accttcaaa gaggggcac gtcgagcta tga
- >TC034370 RB
atggtaatg aaagatcct gccaccagg cggtcgagg ggctttgga tttatcacc ttcaccgac ccggctagc gtagataag gttttagcc caaggcact cacgaacta gatggcaaa aagattgac cccaaagta gcgttcccg agaagagca cacccaaag atggtaacc cggacgaaa aaaatattc gtcggtgga ttatcagcg cccactacc ctagaggac gtcaaaagt tacttcgaa caattcggt ccgattgaa gatgcaatg ctaatgttc gataagcaa acgaatcga cataggggt tttggattt gtcacattt caaagcgaa gatgttgta gataaagtt tgtgaaatc catttccat gagattaac aataaaatg gtggaatgt aagaaagcg caaccaaaa gaagtaatg ctccccgcc aacctagcg aagacacgg gcggcaggc aggggtgct tacgatttt atgtggtca cttggagct cttcctgac ggttttccg gcagccgca tacgcggcc tacgcggcc ggtcgaggt tactcgggc taccctagt ttcggacta ccgtaccca acagtggac ctaaccaac ggacaactg acacctaac aataacacc ccactattg actcaggcc ttgtcagtg atgaataac taccaggct gcggcggcg gcagcgcag ggcttcgga ccgccggct tcgccccat gcagccaac acacgtgga ggatttcct gctgccaac tcacctgga cctaccata gatatgtac aatagcagt gcgggggat agtgtgggc tatgtgcag gccgctagc ccacaaccc tccagcttc ccggcgatc gccgtcagc agggcccct cttaactac agtccgggt cccctaatt cctgctgcg tttaccaac ggctatcac tga
- >TC034370 RC
atggtaatg aaagatcct gccaccagg cggtcgagc acaaattgc gacgggtct gccgtcttc ggcgtcgac agcgtcacc agattgttc acccccagg ggctttgga tttatcacc ttcaccgac ccggctagc gtagataag gttttagcc caaggcact cacgaacta gatggcaaa aagattgac cccaaagta gcgttcccg agaagagca cacccaaag atggtaacc cggacgaaa aaaatattc gtcggtgga ttatcagcg cccactacc ctagaggac gtcaaaagt tacttcgaa caattcggt ccgattgaa gatgcaatg ctaatgttc gataagcaa acgaatcga cataggggt tttggattt gtcacattt caaagcgaa gatgttgta gataaagtt tgtgaaatc catttccat gagattaac aataaaatg gtggaatgt aagaaagcg caaccaaaa gaagtaatg ctccccgcc aacctagcg aagacacgg gcggcaggc aggggtgct tacgatttt atgtggtca cttggagct cttcctgac ggttttccg gcagccgca tacgcggcc tacgcggcc ggtcgaggt tactcgggc taccctagt ttcggacta ccgtaccca acagtggac ctaaccaac ggacaactg acacctaac aataacacc ccactattg actcaggcc ttgtcagtg atgaataac taccaggct gcggcggcg gcagcgcag ggcttcgga ccgccggct tcgccccat gcagccaac acacgtgga ggatttcct gctgccaac tcacctgga cctaccata gatatgtac aatagcagt gcgggggat agtgtgggc tatgtgcag gccgctagc ccacaaccc tccagcttc ccggcgatc gccgtcagc agggcccct cttaactac agtccgggt cccctaatt cctgctgcg tttaccaac ggctatcac tga
- >TC034370 RA
cgagttcaa gaatacaaa gatttcaat ttggtctcg aaattgcct ttcaataaa aaagaaaag tggaaaagt atatgaaca ctaaactag attggcgaa ccgaaaagc ctaagagaa tactttagt aaatacggc gacataaca gaagtaatg gtaatgaaa gatcctgcc accaggcgg tcgaggggc tttggattt atcaccttc accgacccg gctagcgta gataaggtt ttagcccaa ggcactcac gaactagat ggcaaaaag attgacccc aaagtagcg ttcccgaga agagcacac ccaaagatg gtaacccgg acgaaaaaa atattcgtc ggtggatta tcagcgccc actacccta gaggacgtc aaaagttac ttcgaacaa ttcggtccg attgaagat gcaatgcta atgttcgat aagcaaacg aatcgacat aggggtttt ggatttgtc acatttcaa agcgaagat gttgtagat aaagtttgt gaaatccat ttccatgag attaacaat aaaatggtg gaatgtaag aaagcgcaa ccaaaagaa gtaatgctc cccgccaac ctagcgaag acacgggcg gcaggcagg ggtgcttac gattttatg tggtcactt ggagctctt cctgacggt tttccggca gccgcatac gcggcctac gcggccggt cgaggttac tcgggctac cctagtttc ggactaccg tacccaaca gtggaccta accaacgga caactgaca cctaacaat aacacccca ctattgact caggccttg tcagtgatg aataactac caggctgcg gcggcggca gcgcagggc ttcggaccg ccggcttcg ccccatgca gccaacaca cgtggagga tttcctgct gccaactca cctggacct accatagat atgtacaat agcagtgcg ggggatagt gtgggctat gtgcaggcc gctagccca caaccctcc agcttcccg gcgatcgcc gtcagcagg gcccctctt aactacagt ccgaagaca ggaccggcc ggtggctgc atgtctttg ataagatgt ggggccact gcgacggtc gcgtcgagc gctgtccgc gatttgggc acattccag caccgcctc tcgtatacc aacatatca aaggccggt cgcgggctc gctcggacc ttcaaagag gggcacgtc gagctatga ttcaaattt tgattcgcg tgcatattt ttctattag gtacacagt catgtggtg gtgctaatc ggccaaagt ataaaacaa attgacaaa tatttaata aaagaaacg ttgcggatg ttagtttag tgtataggt tgttattga tttttacaa ataaattcg ggttgtact taattgtta ttgagacat tcggcgtgt ccctccgtc ccttagagg ctagaatag gagttatta gtcatttgt aacgtatta tttcccaaa cggaatatt atctcgaca atattgatt cacactgtg aagttttca gttgtatga ttaaaactg tatatcgtg ggccttact atacactga aaattaaat cgtgctgtc caaaattta ttattgtat ttagttcga tcttgcaag cgtatatag tttaggggt ggacgcagg tagtgtagc cgcccccca ccttttgta tacttaagg cgccccgcg ctcttttgt tgtgttact ttcatacgt actttagga ttacgttat taaatgtat acaagctgt atatattga tattatgat gatatacat aacatatac tcttattta attaagcgg tagcgagtg gttaggtgc cgccacgat atgatgtat tatggcacc cgccctgtc cctgtgttg cagcccctg gtccatccg cgctcgggt ctgggccgc tttgtgcgg ggccccatc gtatataat atttagcag cagtcctag tcggtagta gagagcggc ccccgtaga ccggcccaa gccccagcg ctcagtgtt acgtaaact cattgtatt acagccgaa cggcttctt gcaataaaa aagcaataa ccatattcc ttctgtgga cgcttttga cttttacat taagaaaaa tataatatt attattatt aaacaatta ctgtaagta cgtacttta tgaacaaga ttttacact atgataata atgaaggcg atggcatat tgctaatta gaatgattg tacagatag agtttgtaa cataagaaa attatgttg ttcagacga gaggccact caaaatttg gtggtcgct cgcattgct cttgtagcg ctcggcaac ggtccgggc cttcatgta tatctaact acacgttct acaatgtac atataatta ttacaatga taagacaag gttcacaac gtgccgtgt gtatgtggg ataaatata atatggaca ttgtt
- >TC034370 RB
cgagttcaa gaatacaaa gatttcaat ttggtctcg aaattgcct ttcaataaa aaagaaaag tggaaaagt atatgaaca ctaaactag attggcgaa ccgaaaagc ctaagagaa tactttagt aaatacggc gacataaca gaagtaatg gtaatgaaa gatcctgcc accaggcgg tcgaggggc tttggattt atcaccttc accgacccg gctagcgta gataaggtt ttagcccaa ggcactcac gaactagat ggcaaaaag attgacccc aaagtagcg ttcccgaga agagcacac ccaaagatg gtaacccgg acgaaaaaa atattcgtc ggtggatta tcagcgccc actacccta gaggacgtc aaaagttac ttcgaacaa ttcggtccg attgaagat gcaatgcta atgttcgat aagcaaacg aatcgacat aggggtttt ggatttgtc acatttcaa agcgaagat gttgtagat aaagtttgt gaaatccat ttccatgag attaacaat aaaatggtg gaatgtaag aaagcgcaa ccaaaagaa gtaatgctc cccgccaac ctagcgaag acacgggcg gcaggcagg ggtgcttac gattttatg tggtcactt ggagctctt cctgacggt tttccggca gccgcatac gcggcctac gcggccggt cgaggttac tcgggctac cctagtttc ggactaccg tacccaaca gtggaccta accaacgga caactgaca cctaacaat aacacccca ctattgact caggccttg tcagtgatg aataactac caggctgcg gcggcggca gcgcagggc ttcggaccg ccggcttcg ccccatgca gccaacaca cgtggagga tttcctgct gccaactca cctggacct accatagat atgtacaat agcagtgcg ggggatagt gtgggctat gtgcaggcc gctagccca caaccctcc agcttcccg gcgatcgcc gtcagcagg gcccctctt aactacagt ccgggtccc ctaattcct gctgcgttt accaacggc tatcactga gctcgagga ctgtctagt gttgtgtga ctcgactcg gcagctact gtgatgtca tcacaactg cacttgtac attcacttt atgtgtata atataataa tataataat aataatgta
- >TC034370 RC
cgagttcaa gaatacaaa gatttcaat ttggtctcg aaattgcct ttcaataaa aaagaaaag tggaaaagt atatgaaca ctaaactag attggcgaa ccgaaaagc ctaagagaa tactttagt aaatacggc gacataaca gaagtaatg gtaatgaaa gatcctgcc accaggcgg tcgagcaca aattgcgac gggtctgcc gtcttcggc gtcgacagc gtcaccaga ttgttcacc cccaggggc tttggattt atcaccttc accgacccg gctagcgta gataaggtt ttagcccaa ggcactcac gaactagat ggcaaaaag attgacccc aaagtagcg ttcccgaga agagcacac ccaaagatg gtaacccgg acgaaaaaa atattcgtc ggtggatta tcagcgccc actacccta gaggacgtc aaaagttac ttcgaacaa ttcggtccg attgaagat gcaatgcta atgttcgat aagcaaacg aatcgacat aggggtttt ggatttgtc acatttcaa agcgaagat gttgtagat aaagtttgt gaaatccat ttccatgag attaacaat aaaatggtg gaatgtaag aaagcgcaa ccaaaagaa gtaatgctc cccgccaac ctagcgaag acacgggcg gcaggcagg ggtgcttac gattttatg tggtcactt ggagctctt cctgacggt tttccggca gccgcatac gcggcctac gcggccggt cgaggttac tcgggctac cctagtttc ggactaccg tacccaaca gtggaccta accaacgga caactgaca cctaacaat aacacccca ctattgact caggccttg tcagtgatg aataactac caggctgcg gcggcggca gcgcagggc ttcggaccg ccggcttcg ccccatgca gccaacaca cgtggagga tttcctgct gccaactca cctggacct accatagat atgtacaat agcagtgcg ggggatagt gtgggctat gtgcaggcc gctagccca caaccctcc agcttcccg gcgatcgcc gtcagcagg gcccctctt aactacagt ccgggtccc ctaattcct gctgcgttt accaacggc tatcactga gctcgagga ctgtctagt gttgtgtga ctcgactcg gcagctact gtgatgtca tcacaactg cacttgtac attcacttt atgtgtata atataataa tataataat aataatgta
