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Gene information
General information
TC016273 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10241882..10244300
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC016273
      iBeetle screen iB_05985

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016273 RA
        MWKFLLIFA LKTATSDEI FTSTFSVTD QCISLCKEN 
        ELPLLDLEN YEQIVCQRG CRFFNIINF KDELNLNTT 
        RKECHLSCN ESYIVAKEN EICLKGCDL MAKKRQSDM 
        STFLVITED DESHNIVLA EPDLEILES DILSDPSIK 
        SQLEIGFNI DYKIPETHI RTMPIEFKD DTIVIKSSE 
        YSGDWLDCA SRNSGIPRW ILLTAFLSA VLVALWLSF 
        STEKKNTEE VAEVDIPDE KLILENEEK DPELLEDFQ 
        EIGFSTPPK YTVESEKV
      CDS sequence
      • >TC016273 RA
        atgtggaag tttttgtta atttttgcc ttaaaaacc 
        gcaacttct gatgaaatt tttacaagt acgttctca 
        gtgactgat cagtgtatt agtttgtgt aaggaaaat 
        gagctgccg ctgcttgac ttggaaaac tacgagcaa 
        atcgtatgc caacgcggt tgtaggttt tttaacata 
        attaatttt aaggacgag ttgaattta aacaccaca 
        cggaaggag tgccatttg tcatgcaac gaatcttac 
        attgtcgcg aaagaaaac gaaatttgc ttaaaaggt 
        tgcgatttg atggcgaaa aagcgccag agcgacatg 
        tccacattt ctggtgata acagaagat gatgagtcg 
        cataacatt gttttggcg gagcctgac ttagaaata 
        ttagaatct gatattctc tctgacccg agtattaaa 
        agtcagctt gagatcggt ttcaatatt gattacaaa 
        atccccgaa actcacatc agaacaatg cctatcgaa 
        ttcaaggat gacacgatc gtgatcaaa tcgagtgaa 
        tattcaggc gactggctc gattgcgct tccagaaat 
        tcggggatt ccccgatgg attctccta actgcgttt 
        ttgtcagcg gttttagtg gctttatgg ttgagtttc 
        tcaactgaa aagaaaaac actgaggag gtagcggag 
        gtggacatt cctgacgaa aaattaata ctagaaaac 
        gaagaaaaa gatcctgaa cttctggag gattttcaa 
        gaaattgga ttctcgact cctccaaag tacactgtt 
        gaaagtgaa aaggtttaa
      mRNA sequence
      • >TC016273 RA
        cgatagaac aaatcccag aatgtggaa gtttttgtt 
        aatttttgc cttaaaaac cgcaacttc tgatgaaat 
        ttttacaag tacgttctc agtgactga tcagtgtat 
        tagtttgtg taaggaaaa tgagctgcc gctgcttga 
        cttggaaaa ctacgagca aatcgtatg ccaacgcgg 
        ttgtaggtt ttttaacat aattaattt taaggacga 
        gttgaattt aaacaccac acggaagga gtgccattt 
        gtcatgcaa cgaatctta cattgtcgc gaaagaaaa 
        cgaaatttg cttaaaagg ttgcgattt gatggcgaa 
        aaagcgcca gagcgacat gtccacatt tctggtgat 
        aacagaaga tgatgagtc gcataacat tgttttggc 
        ggagcctga cttagaaat attagaatc tgatattct 
        ctctgaccc gagtattaa aagtcagct tgagatcgg 
        tttcaatat tgattacaa aatccccga aactcacat 
        cagaacaat gcctatcga attcaagga tgacacgat 
        cgtgatcaa atcgagtga atattcagg cgactggct 
        cgattgcgc ttccagaaa ttcggggat tccccgatg 
        gattctcct aactgcgtt tttgtcagc ggttttagt 
        ggctttatg gttgagttt ctcaactga aaagaaaaa 
        cactgagga ggtagcgga ggtggacat tcctgacga 
        aaaattaat actagaaaa cgaagaaaa agatcctga 
        acttctgga ggattttca agaaattgg attctcgac 
        tcctccaaa gtacactgt tgaaagtga aaaggttta 
        aaggtagat tttttaaga ggcatattt tcttaagga 
        gggctgatc aggcatagt aattgtgac tgtctcgta 
        aatgtgtag tgggtttat ttcagcatt tgttactta 
        aatgcacag cttacttaa tcagtaaaa taagtttgc 
        gttgtgctt ttgtttcta aggaagtta ctgtggtta 
        tgtgttgct ggtgtaata gatttttca aacaaaagg 
        gcataattt gatttggta acgtggcta taagttttc 
        aggagaaat taacaggtg gcgccacat tgagttagg 
        tcataacca actcgatat gaaattatt acataattt 
        tggcttagc cttcttata tacagatac atttaaata 
        agaaataac atttcagag gaataaagc attaaattt 
        ttaacagta agtaattaa agtaatcca aaaattgtg 
        tcgtaaact atattaaat ttataagaa ttcaaactt 
        ttattattt acatttagt aggggccat tttcataaa 
        caaatttaa atcttcaat taaatttaa gtcgacttt 
        aattctatt ttgttatta cttgaattg tacaattaa 
        aaaattatg tccgtttag gtttaaaat cacaatttt 
        cgtccatta acttattaa atcctccat ttaataaca 
        acgtaaggt aattgaaag taccagttc gttttttgg 
        aagtgaaga tgatgtgtg aaaaaagac gaacaaaat 
        acagtttga gaaataatt acttacaaa tttactaat 
        tatagactg gtttttata agcgtcatt aatttattc 
        cttaattag atacgtgat taactgagc acgtgacca 
        aattgaagt aatttgatt ggtccattt gcgttgtta 
        atttttaac aacgaataa aattttaac agagctttc 
        tataaaccg gcccttatt ttaattgca aaaatacaa 
        tgattagaa aatattaat gaagaagat gatttagaa 
        acgaagatt ttttgataa aacacaaat gctaatgtt 
        agaaaagtt ctaattaca gaccacttt gttgggtta 
        taaaaaatt acttactaa ttattcttt aacattatt 
        ttcttattt tttttttct agatttaga gcatcattt 
        tttggtgtt ctcagtatc ccgcaattt ttttaactg 
        ctttaactt atttctttt cttcctcca cttctttct 
        tgtattttt ttctaactt ttaaacagc tcgtagaat 
        cctcgtaga tagtatttg ttattacct gcaattatt 
        tttctgcct ctgtttttc aaattgtcg aatttggtt 
        ttaatttct gtggcatga attttttaa attaattta 
        tattcaaca tattatatt attcccagg agttgttta 
        aattttgca tcaccatgc cgctagagt aaaaatatt 
        tgaactggt gtaccggaa aaagttaaa aggtggtta 
        tacagggag tttcatgtt ttagtgcca gtcaaaaaa 
        taaacaagt acttttagt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY