| General information | |||
| TC016270 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 10199922..10225727 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC016270 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05983 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC016270 RA
MDGDEDSYC SKSHSRQNG VHVSDLGDM SFMMLDYIK EAMKAMDMM RGHQMLTDI TLEVGHEVF YAHRVVLAA ASPYFKAMF TGGLKECEM TRIPLQGVS ATAMARLIH FMYTGRIRV TENTVCQLL PAATMLQVT NVIQACCDF LERQLDPSN AIGIASFAE QHGCIELCK KANQYIERH FCQICQEEE FLQLNALQL ITLIKKDEL NVQDEKEVY NAVLKWVRH DEENRHKKM EHILSAVRC QFLPPKFLN DQMTNCEVI KKTPACREY LARIFKDLT LHIRTVVKE RTPNIPRVI YVAGGYYRQ SLDVLEGYN IDDKTWHKL DSLTVPRSG LGGAFLKGT FYAVGGRNN APGNSYDSD WVDKYNPVK DQWRPCSPM SVPRNRVGV AVMDGLLYA VGGSEGSRY HNSVECYDP DLDRWTTIK PMHFKRLAV GVAVVNRLL YAIGGYDGT QRHNSAECY HPENNSWTM IAPMHTQRS GAGVAAINQ YIYVVGGYD GSKQLNTVE RYDTEKDTW EFVASMKIA RSALSVTVL DCKIYAMGG YNGQDFLAN VEIYDPLRD VWEDGEPLT SGRSGHTSA VCYQPPCTQ KCRILSQIN NSSSSTSSS TIK
- >TC016270 RB
MISPSDSGW PADLDCRMD GDEDSYCSK SHSRQNGVH VSDLGDMSF MMLDYIKEA MKAMDMMRG HQMLTDITL EVGHEVFYA HRVVLAAAS PYFKAMFTG GLKECEMTR IPLQGVSAT AMARLIHFM YTGRIRVTE NTVCQLLPA ATMLQVTNV IQACCDFLE RQLDPSNAI GIASFAEQH GCIELCKKA NQYIERHFC QICQEEEFL QLNALQLIT LIKKDELNV QDEKEVYNA VLKWVRHDE ENRHKKMEH ILSAVRCQF LPPKFLNDQ MTNCEVIKK TPACREYLA RIFKDLTLH IRTVVKERT PNIPRVIYV AGGYYRQSL DVLEGYNID DKTWHKLDS LTVPRSGLG GAFLKGTFY AVGGRNNAP GNSYDSDWV DKYNPVKDQ WRPCSPMSV PRNRVGVAV MDGLLYAVG GSEGSRYHN SVECYDPDL DRWTTIKPM HFKRLAVGV AVVNRLLYA IGGYDGTQR HNSAECYHP ENNSWTMIA PMHTQRSGA GVAAINQYI YVVGGYDGS KQLNTVERY DTEKDTWEF VASMKIARS ALSVTVLDC KIYAMGGYN GQDFLANVE IYDPLRDVW EDGEPLTSG RSGHTSAVC YQPPCTQKC RILSQINNS SSSTSSSTI K
- >TC016270 RA
atggatgga gacgaagac agttactgc agcaaaagc cattctcgc cagaatggc gtccacgtg tctgacttg ggcgacatg agtttcatg atgttggat tacatcaaa gaagccatg aaggccatg gacatgatg agaggtcat caaatgttg accgacata acactagaa gttggccac gaagtattt tatgcacat cgagtggtc ctcgccgct gctagtccc tactttaag gccatgttt actggtggc ttaaaagaa tgcgaaatg acgaggata ccccttcaa ggcgtttcc gcgaccgcg atggctaga ctaatacat ttcatgtac accggtagg ataagagtg acggaaaac accgtgtgc caattatta cctgccgcg acaatgcta caagttacg aacgtaata caagcctgc tgtgatttt ttagaaaga caattagat cctagcaat gcaattgga attgcaagt tttgcggaa caacacgga tgtatcgaa ttatgcaaa aaggcgaat caatatatc gaacgtcat ttttgccag atctgtcag gaggaggag tttcttcaa ttaaacgcg ttacaatta atcacattg attaagaaa gatgaactt aacgttcaa gacgagaag gaagtttac aacgccgtc ctaaaatgg gtccgacac gacgaagaa aaccgacac aagaaaatg gaacacata ttaagcgca gtccgatgt cagttttta ccacccaag ttcctcaac gaccaaatg actaactgc gaagttatc aaaaaaaca ccggcatgt agagaatat ttagctagg atctttaaa gatttgacg cttcacata cggacagtc gtaaaagaa cgaacacca aatatccct agagtaata tatgtcgca ggcggctac taccgacaa tctctcgac gtcttagag ggttacaat atcgacgat aaaacgtgg cacaaatta gacagtctc actgtacct agatctgga ttaggagga gcttttttg aagggaact ttttacgca gtaggtggc agaaacaac gctccgggt aatagttac gattcggat tgggtggat aaatacaac ccagtcaag gaccaatgg agaccatgt agccccatg tcggtgccg cgaaatcga gtaggtgtt gctgtaatg gacggcttg ctgtacgca gttggcgga agtgagggc tccagatac cacaatagc gtcgaatgc tacgatcct gatttggat aggtggacg acgataaaa cctatgcat ttcaaacgt ctagcggtt ggtgtcgct gttgtcaac cgactgcta tatgcaata ggtggttat gacggtacc caaagacac aatagtgca gagtgttac caccccgaa aataactcg tggactatg atagccccc atgcatacg caacggagc ggcgctgga gttgccgca attaatcag tacatatac gtagttggt ggatatgac ggctcgaag caactgaat acagtggaa agatatgat acggagaaa gatacttgg gaatttgtt gctagtatg aagattgca agaagtgcc ctaagtgta acggtccta gattgcaaa atatacgca atgggtgga tataatggt caggatttt ttagcgaac gtagaaatt tatgatcct ttgagagac gtatgggag gatggggaa ccgttgacc tcgggtcga tcaggtcat actagtgct gtttgttac caaccacct tgtactcag aagtgtagg attttatcg caaataaac aatagttcg agttcgacg tcatcttcc accattaag taa
- >TC016270 RB
atgatttct cccagcgat agtggttgg ccggcagat ttagattgt agaatggat ggagacgaa gacagttac tgcagcaaa agccattct cgccagaat ggcgtccac gtgtctgac ttgggcgac atgagtttc atgatgttg gattacatc aaagaagcc atgaaggcc atggacatg atgagaggt catcaaatg ttgaccgac ataacacta gaagttggc cacgaagta ttttatgca catcgagtg gtcctcgcc gctgctagt ccctacttt aaggccatg tttactggt ggcttaaaa gaatgcgaa atgacgagg atacccctt caaggcgtt tccgcgacc gcgatggct agactaata catttcatg tacaccggt aggataaga gtgacggaa aacaccgtg tgccaatta ttacctgcc gcgacaatg ctacaagtt acgaacgta atacaagcc tgctgtgat tttttagaa agacaatta gatcctagc aatgcaatt ggaattgca agttttgcg gaacaacac ggatgtatc gaattatgc aaaaaggcg aatcaatat atcgaacgt catttttgc cagatctgt caggaggag gagtttctt caattaaac gcgttacaa ttaatcaca ttgattaag aaagatgaa cttaacgtt caagacgag aaggaagtt tacaacgcc gtcctaaaa tgggtccga cacgacgaa gaaaaccga cacaagaaa atggaacac atattaagc gcagtccga tgtcagttt ttaccaccc aagttcctc aacgaccaa atgactaac tgcgaagtt atcaaaaaa acaccggca tgtagagaa tatttagct aggatcttt aaagatttg acgcttcac atacggaca gtcgtaaaa gaacgaaca ccaaatatc cctagagta atatatgtc gcaggcggc tactaccga caatctctc gacgtctta gagggttac aatatcgac gataaaacg tggcacaaa ttagacagt ctcactgta cctagatct ggattagga ggagctttt ttgaaggga actttttac gcagtaggt ggcagaaac aacgctccg ggtaatagt tacgattcg gattgggtg gataaatac aacccagtc aaggaccaa tggagacca tgtagcccc atgtcggtg ccgcgaaat cgagtaggt gttgctgta atggacggc ttgctgtac gcagttggc ggaagtgag ggctccaga taccacaat agcgtcgaa tgctacgat cctgatttg gataggtgg acgacgata aaacctatg catttcaaa cgtctagcg gttggtgtc gctgttgtc aaccgactg ctatatgca ataggtggt tatgacggt acccaaaga cacaatagt gcagagtgt taccacccc gaaaataac tcgtggact atgatagcc cccatgcat acgcaacgg agcggcgct ggagttgcc gcaattaat cagtacata tacgtagtt ggtggatat gacggctcg aagcaactg aatacagtg gaaagatat gatacggag aaagatact tgggaattt gttgctagt atgaagatt gcaagaagt gccctaagt gtaacggtc ctagattgc aaaatatac gcaatgggt ggatataat ggtcaggat tttttagcg aacgtagaa atttatgat cctttgaga gacgtatgg gaggatggg gaaccgttg acctcgggt cgatcaggt catactagt gctgtttgt taccaacca ccttgtact cagaagtgt aggatttta tcgcaaata aacaatagt tcgagttcg acgtcatct tccaccatt aagtaa
- >TC016270 RA
gtttgttat gaaacaatt gtgtaacat tctgcatgc gtcaactcc gatctcgga ctctcgact agtagtgtc agaatgtca ctgtcattt tgtgcggtt attgaaatg tcaaatgtg taataataa acattgctg aaactgatg ttaagtgtt gtttatctc taacatatt tgcacgtgc caatcccga aaaataatt gggaaaata ataccaaag cgttgtaga atggatgga gacgaagac agttactgc agcaaaagc cattctcgc cagaatggc gtccacgtg tctgacttg ggcgacatg agtttcatg atgttggat tacatcaaa gaagccatg aaggccatg gacatgatg agaggtcat caaatgttg accgacata acactagaa gttggccac gaagtattt tatgcacat cgagtggtc ctcgccgct gctagtccc tactttaag gccatgttt actggtggc ttaaaagaa tgcgaaatg acgaggata ccccttcaa ggcgtttcc gcgaccgcg atggctaga ctaatacat ttcatgtac accggtagg ataagagtg acggaaaac accgtgtgc caattatta cctgccgcg acaatgcta caagttacg aacgtaata caagcctgc tgtgatttt ttagaaaga caattagat cctagcaat gcaattgga attgcaagt tttgcggaa caacacgga tgtatcgaa ttatgcaaa aaggcgaat caatatatc gaacgtcat ttttgccag atctgtcag gaggaggag tttcttcaa ttaaacgcg ttacaatta atcacattg attaagaaa gatgaactt aacgttcaa gacgagaag gaagtttac aacgccgtc ctaaaatgg gtccgacac gacgaagaa aaccgacac aagaaaatg gaacacata ttaagcgca gtccgatgt cagttttta ccacccaag ttcctcaac gaccaaatg actaactgc gaagttatc aaaaaaaca ccggcatgt agagaatat ttagctagg atctttaaa gatttgacg cttcacata cggacagtc gtaaaagaa cgaacacca aatatccct agagtaata tatgtcgca ggcggctac taccgacaa tctctcgac gtcttagag ggttacaat atcgacgat aaaacgtgg cacaaatta gacagtctc actgtacct agatctgga ttaggagga gcttttttg aagggaact ttttacgca gtaggtggc agaaacaac gctccgggt aatagttac gattcggat tgggtggat aaatacaac ccagtcaag gaccaatgg agaccatgt agccccatg tcggtgccg cgaaatcga gtaggtgtt gctgtaatg gacggcttg ctgtacgca gttggcgga agtgagggc tccagatac cacaatagc gtcgaatgc tacgatcct gatttggat aggtggacg acgataaaa cctatgcat ttcaaacgt ctagcggtt ggtgtcgct gttgtcaac cgactgcta tatgcaata ggtggttat gacggtacc caaagacac aatagtgca gagtgttac caccccgaa aataactcg tggactatg atagccccc atgcatacg caacggagc ggcgctgga gttgccgca attaatcag tacatatac gtagttggt ggatatgac ggctcgaag caactgaat acagtggaa agatatgat acggagaaa gatacttgg gaatttgtt gctagtatg aagattgca agaagtgcc ctaagtgta acggtccta gattgcaaa atatacgca atgggtgga tataatggt caggatttt ttagcgaac gtagaaatt tatgatcct ttgagagac gtatgggag gatggggaa ccgttgacc tcgggtcga tcaggtcat actagtgct gtttgttac caaccacct tgtactcag aagtgtagg attttatcg caaataaac aatagttcg agttcgacg tcatcttcc accattaag taataaagg cgttatgtc ggattaaaa ataataata ataatcttt acctgactg aaaataggt taaacgtgc atttttgtt gcatcagtt cagttatac atctcaata tatgtgata atatttgtc agaagtgtg tatggagtc tattgtttt gttaagaac agaaaaaat gacggtatt taataattt atttatgta aatgaagat gtgcgtgtg tgagttttc ttatgattt tgtagatac tttaaaccg atttaaaaa tctattgtt cttgaaagt tactgtgag acagtattt ataaatgtt gaataaaac attcccgtt gcc
- >TC016270 RB
gtttgttat gaaacaatt gtgtaacat tctgcatgc gtcaactcc gatctcgga ctctcgact agtagtgtc agaatgtca ctgtcattt tgtgcggtt attgaaatg tcaaatgtg taataataa acattgctg aaactgatg ttaagtgtt gtttatctc taacatatt tgcacgtgc caatcccga aaaataatt gggaaaata ataccaaag cgttgtgga gtaacggtg gagcatgga ggaccattg ttcgagctg tgatatatt agtgcgtaa tgtgtagat aaaaaaaat gatttctcc cagcgatag tggttggcc ggcagattt agattgtag aatggatgg agacgaaga cagttactg cagcaaaag ccattctcg ccagaatgg cgtccacgt gtctgactt gggcgacat gagtttcat gatgttgga ttacatcaa agaagccat gaaggccat ggacatgat gagaggtca tcaaatgtt gaccgacat aacactaga agttggcca cgaagtatt ttatgcaca tcgagtggt cctcgccgc tgctagtcc ctactttaa ggccatgtt tactggtgg cttaaaaga atgcgaaat gacgaggat accccttca aggcgtttc cgcgaccgc gatggctag actaataca tttcatgta caccggtag gataagagt gacggaaaa caccgtgtg ccaattatt acctgccgc gacaatgct acaagttac gaacgtaat acaagcctg ctgtgattt tttagaaag acaattaga tcctagcaa tgcaattgg aattgcaag ttttgcgga acaacacgg atgtatcga attatgcaa aaaggcgaa tcaatatat cgaacgtca tttttgcca gatctgtca ggaggagga gtttcttca attaaacgc gttacaatt aatcacatt gattaagaa agatgaact taacgttca agacgagaa ggaagttta caacgccgt cctaaaatg ggtccgaca cgacgaaga aaaccgaca caagaaaat ggaacacat attaagcgc agtccgatg tcagttttt accacccaa gttcctcaa cgaccaaat gactaactg cgaagttat caaaaaaac accggcatg tagagaata tttagctag gatctttaa agatttgac gcttcacat acggacagt cgtaaaaga acgaacacc aaatatccc tagagtaat atatgtcgc aggcggcta ctaccgaca atctctcga cgtcttaga gggttacaa tatcgacga taaaacgtg gcacaaatt agacagtct cactgtacc tagatctgg attaggagg agctttttt gaagggaac tttttacgc agtaggtgg cagaaacaa cgctccggg taatagtta cgattcgga ttgggtgga taaatacaa cccagtcaa ggaccaatg gagaccatg tagccccat gtcggtgcc gcgaaatcg agtaggtgt tgctgtaat ggacggctt gctgtacgc agttggcgg aagtgaggg ctccagata ccacaatag cgtcgaatg ctacgatcc tgatttgga taggtggac gacgataaa acctatgca tttcaaacg tctagcggt tggtgtcgc tgttgtcaa ccgactgct atatgcaat aggtggtta tgacggtac ccaaagaca caatagtgc agagtgtta ccaccccga aaataactc gtggactat gatagcccc catgcatac gcaacggag cggcgctgg agttgccgc aattaatca gtacatata cgtagttgg tggatatga cggctcgaa gcaactgaa tacagtgga aagatatga tacggagaa agatacttg ggaatttgt tgctagtat gaagattgc aagaagtgc cctaagtgt aacggtcct agattgcaa aatatacgc aatgggtgg atataatgg tcaggattt tttagcgaa cgtagaaat ttatgatcc tttgagaga cgtatggga ggatgggga accgttgac ctcgggtcg atcaggtca tactagtgc tgtttgtta ccaaccacc ttgtactca gaagtgtag gattttatc gcaaataaa caatagttc gagttcgac gtcatcttc caccattaa gtaataaag gcgttatgt cggattaaa aataataat aataatctt tacctgact gaaaatagg ttaaacgtg catttttgt tgcatcagt tcagttata catctcaat atatgtgat aatatttgt cagaagtgt gtatggagt ctattgttt tgttaagaa cagaaaaaa tgacggtat ttaataatt tatttatgt aaatgaaga tgtgcgtgt gtgagtttt cttatgatt ttgtagata ctttaaacc gatttaaaa atctattgt tcttgaaag ttactgtga gacagtatt tataaatgt tgaataaaa cattcccgt tgcc
