Status
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Gene information
General information
TC016270 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10199922..10225727
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016270
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016270 RA
      MDGDEDSYC SKSHSRQNG VHVSDLGDM SFMMLDYIK 
      EAMKAMDMM RGHQMLTDI TLEVGHEVF YAHRVVLAA 
      ASPYFKAMF TGGLKECEM TRIPLQGVS ATAMARLIH 
      FMYTGRIRV TENTVCQLL PAATMLQVT NVIQACCDF 
      LERQLDPSN AIGIASFAE QHGCIELCK KANQYIERH 
      FCQICQEEE FLQLNALQL ITLIKKDEL NVQDEKEVY 
      NAVLKWVRH DEENRHKKM EHILSAVRC QFLPPKFLN 
      DQMTNCEVI KKTPACREY LARIFKDLT LHIRTVVKE 
      RTPNIPRVI YVAGGYYRQ SLDVLEGYN IDDKTWHKL 
      DSLTVPRSG LGGAFLKGT FYAVGGRNN APGNSYDSD 
      WVDKYNPVK DQWRPCSPM SVPRNRVGV AVMDGLLYA 
      VGGSEGSRY HNSVECYDP DLDRWTTIK PMHFKRLAV 
      GVAVVNRLL YAIGGYDGT QRHNSAECY HPENNSWTM 
      IAPMHTQRS GAGVAAINQ YIYVVGGYD GSKQLNTVE 
      RYDTEKDTW EFVASMKIA RSALSVTVL DCKIYAMGG 
      YNGQDFLAN VEIYDPLRD VWEDGEPLT SGRSGHTSA 
      VCYQPPCTQ KCRILSQIN NSSSSTSSS TIK
    • >TC016270 RB
      MISPSDSGW PADLDCRMD GDEDSYCSK SHSRQNGVH 
      VSDLGDMSF MMLDYIKEA MKAMDMMRG HQMLTDITL 
      EVGHEVFYA HRVVLAAAS PYFKAMFTG GLKECEMTR 
      IPLQGVSAT AMARLIHFM YTGRIRVTE NTVCQLLPA 
      ATMLQVTNV IQACCDFLE RQLDPSNAI GIASFAEQH 
      GCIELCKKA NQYIERHFC QICQEEEFL QLNALQLIT 
      LIKKDELNV QDEKEVYNA VLKWVRHDE ENRHKKMEH 
      ILSAVRCQF LPPKFLNDQ MTNCEVIKK TPACREYLA 
      RIFKDLTLH IRTVVKERT PNIPRVIYV AGGYYRQSL 
      DVLEGYNID DKTWHKLDS LTVPRSGLG GAFLKGTFY 
      AVGGRNNAP GNSYDSDWV DKYNPVKDQ WRPCSPMSV 
      PRNRVGVAV MDGLLYAVG GSEGSRYHN SVECYDPDL 
      DRWTTIKPM HFKRLAVGV AVVNRLLYA IGGYDGTQR 
      HNSAECYHP ENNSWTMIA PMHTQRSGA GVAAINQYI 
      YVVGGYDGS KQLNTVERY DTEKDTWEF VASMKIARS 
      ALSVTVLDC KIYAMGGYN GQDFLANVE IYDPLRDVW 
      EDGEPLTSG RSGHTSAVC YQPPCTQKC RILSQINNS 
      SSSTSSSTI K
    CDS sequence
    • >TC016270 RA
      atggatgga gacgaagac agttactgc agcaaaagc 
      cattctcgc cagaatggc gtccacgtg tctgacttg 
      ggcgacatg agtttcatg atgttggat tacatcaaa 
      gaagccatg aaggccatg gacatgatg agaggtcat 
      caaatgttg accgacata acactagaa gttggccac 
      gaagtattt tatgcacat cgagtggtc ctcgccgct 
      gctagtccc tactttaag gccatgttt actggtggc 
      ttaaaagaa tgcgaaatg acgaggata ccccttcaa 
      ggcgtttcc gcgaccgcg atggctaga ctaatacat 
      ttcatgtac accggtagg ataagagtg acggaaaac 
      accgtgtgc caattatta cctgccgcg acaatgcta 
      caagttacg aacgtaata caagcctgc tgtgatttt 
      ttagaaaga caattagat cctagcaat gcaattgga 
      attgcaagt tttgcggaa caacacgga tgtatcgaa 
      ttatgcaaa aaggcgaat caatatatc gaacgtcat 
      ttttgccag atctgtcag gaggaggag tttcttcaa 
      ttaaacgcg ttacaatta atcacattg attaagaaa 
      gatgaactt aacgttcaa gacgagaag gaagtttac 
      aacgccgtc ctaaaatgg gtccgacac gacgaagaa 
      aaccgacac aagaaaatg gaacacata ttaagcgca 
      gtccgatgt cagttttta ccacccaag ttcctcaac 
      gaccaaatg actaactgc gaagttatc aaaaaaaca 
      ccggcatgt agagaatat ttagctagg atctttaaa 
      gatttgacg cttcacata cggacagtc gtaaaagaa 
      cgaacacca aatatccct agagtaata tatgtcgca 
      ggcggctac taccgacaa tctctcgac gtcttagag 
      ggttacaat atcgacgat aaaacgtgg cacaaatta 
      gacagtctc actgtacct agatctgga ttaggagga 
      gcttttttg aagggaact ttttacgca gtaggtggc 
      agaaacaac gctccgggt aatagttac gattcggat 
      tgggtggat aaatacaac ccagtcaag gaccaatgg 
      agaccatgt agccccatg tcggtgccg cgaaatcga 
      gtaggtgtt gctgtaatg gacggcttg ctgtacgca 
      gttggcgga agtgagggc tccagatac cacaatagc 
      gtcgaatgc tacgatcct gatttggat aggtggacg 
      acgataaaa cctatgcat ttcaaacgt ctagcggtt 
      ggtgtcgct gttgtcaac cgactgcta tatgcaata 
      ggtggttat gacggtacc caaagacac aatagtgca 
      gagtgttac caccccgaa aataactcg tggactatg 
      atagccccc atgcatacg caacggagc ggcgctgga 
      gttgccgca attaatcag tacatatac gtagttggt 
      ggatatgac ggctcgaag caactgaat acagtggaa 
      agatatgat acggagaaa gatacttgg gaatttgtt 
      gctagtatg aagattgca agaagtgcc ctaagtgta 
      acggtccta gattgcaaa atatacgca atgggtgga 
      tataatggt caggatttt ttagcgaac gtagaaatt 
      tatgatcct ttgagagac gtatgggag gatggggaa 
      ccgttgacc tcgggtcga tcaggtcat actagtgct 
      gtttgttac caaccacct tgtactcag aagtgtagg 
      attttatcg caaataaac aatagttcg agttcgacg 
      tcatcttcc accattaag taa
    • >TC016270 RB
      atgatttct cccagcgat agtggttgg ccggcagat 
      ttagattgt agaatggat ggagacgaa gacagttac 
      tgcagcaaa agccattct cgccagaat ggcgtccac 
      gtgtctgac ttgggcgac atgagtttc atgatgttg 
      gattacatc aaagaagcc atgaaggcc atggacatg 
      atgagaggt catcaaatg ttgaccgac ataacacta 
      gaagttggc cacgaagta ttttatgca catcgagtg 
      gtcctcgcc gctgctagt ccctacttt aaggccatg 
      tttactggt ggcttaaaa gaatgcgaa atgacgagg 
      atacccctt caaggcgtt tccgcgacc gcgatggct 
      agactaata catttcatg tacaccggt aggataaga 
      gtgacggaa aacaccgtg tgccaatta ttacctgcc 
      gcgacaatg ctacaagtt acgaacgta atacaagcc 
      tgctgtgat tttttagaa agacaatta gatcctagc 
      aatgcaatt ggaattgca agttttgcg gaacaacac 
      ggatgtatc gaattatgc aaaaaggcg aatcaatat 
      atcgaacgt catttttgc cagatctgt caggaggag 
      gagtttctt caattaaac gcgttacaa ttaatcaca 
      ttgattaag aaagatgaa cttaacgtt caagacgag 
      aaggaagtt tacaacgcc gtcctaaaa tgggtccga 
      cacgacgaa gaaaaccga cacaagaaa atggaacac 
      atattaagc gcagtccga tgtcagttt ttaccaccc 
      aagttcctc aacgaccaa atgactaac tgcgaagtt 
      atcaaaaaa acaccggca tgtagagaa tatttagct 
      aggatcttt aaagatttg acgcttcac atacggaca 
      gtcgtaaaa gaacgaaca ccaaatatc cctagagta 
      atatatgtc gcaggcggc tactaccga caatctctc 
      gacgtctta gagggttac aatatcgac gataaaacg 
      tggcacaaa ttagacagt ctcactgta cctagatct 
      ggattagga ggagctttt ttgaaggga actttttac 
      gcagtaggt ggcagaaac aacgctccg ggtaatagt 
      tacgattcg gattgggtg gataaatac aacccagtc 
      aaggaccaa tggagacca tgtagcccc atgtcggtg 
      ccgcgaaat cgagtaggt gttgctgta atggacggc 
      ttgctgtac gcagttggc ggaagtgag ggctccaga 
      taccacaat agcgtcgaa tgctacgat cctgatttg 
      gataggtgg acgacgata aaacctatg catttcaaa 
      cgtctagcg gttggtgtc gctgttgtc aaccgactg 
      ctatatgca ataggtggt tatgacggt acccaaaga 
      cacaatagt gcagagtgt taccacccc gaaaataac 
      tcgtggact atgatagcc cccatgcat acgcaacgg 
      agcggcgct ggagttgcc gcaattaat cagtacata 
      tacgtagtt ggtggatat gacggctcg aagcaactg 
      aatacagtg gaaagatat gatacggag aaagatact 
      tgggaattt gttgctagt atgaagatt gcaagaagt 
      gccctaagt gtaacggtc ctagattgc aaaatatac 
      gcaatgggt ggatataat ggtcaggat tttttagcg 
      aacgtagaa atttatgat cctttgaga gacgtatgg 
      gaggatggg gaaccgttg acctcgggt cgatcaggt 
      catactagt gctgtttgt taccaacca ccttgtact 
      cagaagtgt aggatttta tcgcaaata aacaatagt 
      tcgagttcg acgtcatct tccaccatt aagtaa
    mRNA sequence
    • >TC016270 RA
      gtttgttat gaaacaatt gtgtaacat tctgcatgc 
      gtcaactcc gatctcgga ctctcgact agtagtgtc 
      agaatgtca ctgtcattt tgtgcggtt attgaaatg 
      tcaaatgtg taataataa acattgctg aaactgatg 
      ttaagtgtt gtttatctc taacatatt tgcacgtgc 
      caatcccga aaaataatt gggaaaata ataccaaag 
      cgttgtaga atggatgga gacgaagac agttactgc 
      agcaaaagc cattctcgc cagaatggc gtccacgtg 
      tctgacttg ggcgacatg agtttcatg atgttggat 
      tacatcaaa gaagccatg aaggccatg gacatgatg 
      agaggtcat caaatgttg accgacata acactagaa 
      gttggccac gaagtattt tatgcacat cgagtggtc 
      ctcgccgct gctagtccc tactttaag gccatgttt 
      actggtggc ttaaaagaa tgcgaaatg acgaggata 
      ccccttcaa ggcgtttcc gcgaccgcg atggctaga 
      ctaatacat ttcatgtac accggtagg ataagagtg 
      acggaaaac accgtgtgc caattatta cctgccgcg 
      acaatgcta caagttacg aacgtaata caagcctgc 
      tgtgatttt ttagaaaga caattagat cctagcaat 
      gcaattgga attgcaagt tttgcggaa caacacgga 
      tgtatcgaa ttatgcaaa aaggcgaat caatatatc 
      gaacgtcat ttttgccag atctgtcag gaggaggag 
      tttcttcaa ttaaacgcg ttacaatta atcacattg 
      attaagaaa gatgaactt aacgttcaa gacgagaag 
      gaagtttac aacgccgtc ctaaaatgg gtccgacac 
      gacgaagaa aaccgacac aagaaaatg gaacacata 
      ttaagcgca gtccgatgt cagttttta ccacccaag 
      ttcctcaac gaccaaatg actaactgc gaagttatc 
      aaaaaaaca ccggcatgt agagaatat ttagctagg 
      atctttaaa gatttgacg cttcacata cggacagtc 
      gtaaaagaa cgaacacca aatatccct agagtaata 
      tatgtcgca ggcggctac taccgacaa tctctcgac 
      gtcttagag ggttacaat atcgacgat aaaacgtgg 
      cacaaatta gacagtctc actgtacct agatctgga 
      ttaggagga gcttttttg aagggaact ttttacgca 
      gtaggtggc agaaacaac gctccgggt aatagttac 
      gattcggat tgggtggat aaatacaac ccagtcaag 
      gaccaatgg agaccatgt agccccatg tcggtgccg 
      cgaaatcga gtaggtgtt gctgtaatg gacggcttg 
      ctgtacgca gttggcgga agtgagggc tccagatac 
      cacaatagc gtcgaatgc tacgatcct gatttggat 
      aggtggacg acgataaaa cctatgcat ttcaaacgt 
      ctagcggtt ggtgtcgct gttgtcaac cgactgcta 
      tatgcaata ggtggttat gacggtacc caaagacac 
      aatagtgca gagtgttac caccccgaa aataactcg 
      tggactatg atagccccc atgcatacg caacggagc 
      ggcgctgga gttgccgca attaatcag tacatatac 
      gtagttggt ggatatgac ggctcgaag caactgaat 
      acagtggaa agatatgat acggagaaa gatacttgg 
      gaatttgtt gctagtatg aagattgca agaagtgcc 
      ctaagtgta acggtccta gattgcaaa atatacgca 
      atgggtgga tataatggt caggatttt ttagcgaac 
      gtagaaatt tatgatcct ttgagagac gtatgggag 
      gatggggaa ccgttgacc tcgggtcga tcaggtcat 
      actagtgct gtttgttac caaccacct tgtactcag 
      aagtgtagg attttatcg caaataaac aatagttcg 
      agttcgacg tcatcttcc accattaag taataaagg 
      cgttatgtc ggattaaaa ataataata ataatcttt 
      acctgactg aaaataggt taaacgtgc atttttgtt 
      gcatcagtt cagttatac atctcaata tatgtgata 
      atatttgtc agaagtgtg tatggagtc tattgtttt 
      gttaagaac agaaaaaat gacggtatt taataattt 
      atttatgta aatgaagat gtgcgtgtg tgagttttc 
      ttatgattt tgtagatac tttaaaccg atttaaaaa 
      tctattgtt cttgaaagt tactgtgag acagtattt 
      ataaatgtt gaataaaac attcccgtt gcc
    • >TC016270 RB
      gtttgttat gaaacaatt gtgtaacat tctgcatgc 
      gtcaactcc gatctcgga ctctcgact agtagtgtc 
      agaatgtca ctgtcattt tgtgcggtt attgaaatg 
      tcaaatgtg taataataa acattgctg aaactgatg 
      ttaagtgtt gtttatctc taacatatt tgcacgtgc 
      caatcccga aaaataatt gggaaaata ataccaaag 
      cgttgtgga gtaacggtg gagcatgga ggaccattg 
      ttcgagctg tgatatatt agtgcgtaa tgtgtagat 
      aaaaaaaat gatttctcc cagcgatag tggttggcc 
      ggcagattt agattgtag aatggatgg agacgaaga 
      cagttactg cagcaaaag ccattctcg ccagaatgg 
      cgtccacgt gtctgactt gggcgacat gagtttcat 
      gatgttgga ttacatcaa agaagccat gaaggccat 
      ggacatgat gagaggtca tcaaatgtt gaccgacat 
      aacactaga agttggcca cgaagtatt ttatgcaca 
      tcgagtggt cctcgccgc tgctagtcc ctactttaa 
      ggccatgtt tactggtgg cttaaaaga atgcgaaat 
      gacgaggat accccttca aggcgtttc cgcgaccgc 
      gatggctag actaataca tttcatgta caccggtag 
      gataagagt gacggaaaa caccgtgtg ccaattatt 
      acctgccgc gacaatgct acaagttac gaacgtaat 
      acaagcctg ctgtgattt tttagaaag acaattaga 
      tcctagcaa tgcaattgg aattgcaag ttttgcgga 
      acaacacgg atgtatcga attatgcaa aaaggcgaa 
      tcaatatat cgaacgtca tttttgcca gatctgtca 
      ggaggagga gtttcttca attaaacgc gttacaatt 
      aatcacatt gattaagaa agatgaact taacgttca 
      agacgagaa ggaagttta caacgccgt cctaaaatg 
      ggtccgaca cgacgaaga aaaccgaca caagaaaat 
      ggaacacat attaagcgc agtccgatg tcagttttt 
      accacccaa gttcctcaa cgaccaaat gactaactg 
      cgaagttat caaaaaaac accggcatg tagagaata 
      tttagctag gatctttaa agatttgac gcttcacat 
      acggacagt cgtaaaaga acgaacacc aaatatccc 
      tagagtaat atatgtcgc aggcggcta ctaccgaca 
      atctctcga cgtcttaga gggttacaa tatcgacga 
      taaaacgtg gcacaaatt agacagtct cactgtacc 
      tagatctgg attaggagg agctttttt gaagggaac 
      tttttacgc agtaggtgg cagaaacaa cgctccggg 
      taatagtta cgattcgga ttgggtgga taaatacaa 
      cccagtcaa ggaccaatg gagaccatg tagccccat 
      gtcggtgcc gcgaaatcg agtaggtgt tgctgtaat 
      ggacggctt gctgtacgc agttggcgg aagtgaggg 
      ctccagata ccacaatag cgtcgaatg ctacgatcc 
      tgatttgga taggtggac gacgataaa acctatgca 
      tttcaaacg tctagcggt tggtgtcgc tgttgtcaa 
      ccgactgct atatgcaat aggtggtta tgacggtac 
      ccaaagaca caatagtgc agagtgtta ccaccccga 
      aaataactc gtggactat gatagcccc catgcatac 
      gcaacggag cggcgctgg agttgccgc aattaatca 
      gtacatata cgtagttgg tggatatga cggctcgaa 
      gcaactgaa tacagtgga aagatatga tacggagaa 
      agatacttg ggaatttgt tgctagtat gaagattgc 
      aagaagtgc cctaagtgt aacggtcct agattgcaa 
      aatatacgc aatgggtgg atataatgg tcaggattt 
      tttagcgaa cgtagaaat ttatgatcc tttgagaga 
      cgtatggga ggatgggga accgttgac ctcgggtcg 
      atcaggtca tactagtgc tgtttgtta ccaaccacc 
      ttgtactca gaagtgtag gattttatc gcaaataaa 
      caatagttc gagttcgac gtcatcttc caccattaa 
      gtaataaag gcgttatgt cggattaaa aataataat 
      aataatctt tacctgact gaaaatagg ttaaacgtg 
      catttttgt tgcatcagt tcagttata catctcaat 
      atatgtgat aatatttgt cagaagtgt gtatggagt 
      ctattgttt tgttaagaa cagaaaaaa tgacggtat 
      ttaataatt tatttatgt aaatgaaga tgtgcgtgt 
      gtgagtttt cttatgatt ttgtagata ctttaaacc 
      gatttaaaa atctattgt tcttgaaag ttactgtga 
      gacagtatt tataaatgt tgaataaaa cattcccgt 
      tgcc
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