| General information | |||
| TC016256 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450232.1: 259002..303305 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC016256 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05982 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC016256 RA
MNEVGSLGI RGLAVSPDS DCNSNHHGS ASYLSNTSS QDIDFIQDN SDYQWFLDY GYRDGGTNH HTSILSLPE SYEANDMNY YDALAKNMD ANLAEADME SFRTEDIHA LLTNLPPMC TDHLSQENN RQGESYASV SGSMMAKFD FDSSLSPHS SSHGEDSAN STSMSIYKS ELLFSPVKE TPMPGANFS VDSLDCDMM LTCQANKDN YTIAFEGST LYSEDSDYH DDKSENSGM GTHWVKKPT NNCKLTEAS MTQSDSSSF TTWSKLKKR SSDNQLRRH PSGNNNNSG DETSLTENS AKSQSMPNL YKKKLNYLK NHNFVSTET IRRKPVKVF DIQHQSSSV SDMATSIDA STSETTTNK HQHNFSLVK LFMKQKSMS TEGMSTTMD QLSSTENWP GSQSGESNS SDSKPELDQ KTNESSVRT YDLIAEEPE EEVSRSESV EQLSESISK IDYSDENVN NNNTLKHEL MNQSIQTSQ FSEITNLKN IAKPATKPV KIVEPSFLN KLKQEGEVQ KPVYVLYPN YVLPNLDFL NKQDDMTKV LLIPQEPNL KTVNKRRPF SCTDLEMLK KKGFSHVKD WDSLNFLLP QEYRKILAE VPEISENIK KSRKRRPFS CDYSSSSSS TATQPSSGY RGSSTMLSD SQNSPATNL NPLFVYRYD SVTSSEASL LNSGQRSIM TAPPLPVRC VSLQQGEVP PRPPLPKGI LRKVDAKRF SLYETSEED LTEEGMYRR NILRKKRFS ETEDEGVDA GTSSSSVDE HHEPPKTDF LLSNISTDE LVQLEEFLK ISGISTSDN EEMTDESLM QLRSYVGKF LSLKINQDG PGKKTVSFA EKVNVLPKQ FTAPNNSPN VSKNYFGKN LAEMPICEE SENSNYSPV HKPNLVEKR SLVNAVTDS VEQLINHFS SACNQSELN TLGDSGATP NLAKLALST LCPALYAVL SDGLKPSLE SSFGAINNS VWQVVEASA QQGPLTKAL NELVMRINS EDAITEGLV KFNAFVFGL LNVRSLDAW VSYLKTRES ILRKHYNKD SLIMAHVGG LTLRTLLDS LISALQPLA LLPFQLDLL FEYRQLHLS FKRLEQLSP THKNRTSSS SDDVSVVTV IPDLLSSSK KKERPRSCV ESGSLPKPN FKLGDVTTA AKKRWSGIS SNSKLYLVY DRLAREDEE EYTDSLENQ TKPDDGEPP ESLDSNISD DKPINGRRF KKLQQKWEM LSGRESNPS PPSSPTNNK SKIPRPITS PIKPSGIPI LISPSGKSQ KTPKKNLVQ AKPPPKPKK TTSVRTSRV DQVESETPR HVPRPSSLP YKAMTAQKV AKVTPPRRA ASSSLSRKP VGKSNVPKV VRTLSHRVP SESGHLSYN EGERLKVVL EVDDKWLLC CRGSQKGLV PKSAVISAD SRF
- >TC016256 RA
atgaacgag gtggggagt ctgggtatt cgagggctt gctgtcagc ccagactct gactgcaac agcaaccac cacggctca gcaagttac ctcagcaat acgtcttcc caagatata gatttcatt caagataac tctgattac caatggttt ctagactat ggctatcgg gatggcgga accaatcat cacacgagt attctgtca cttcccgag tcgtacgaa gccaacgat atgaattat tatgacgct ctggcaaag aacatggac gcgaatttg gcagaagcc gatatggag agttttcgg acggaggat attcacgca cttttgact aacttaccg ccaatgtgc accgaccat ttgagtcaa gaaaataat cggcaagga gagagttat gcaagtgtc tcgggttca atgatggca aaatttgat ttcgacagc tcgttaagt cctcatagt agttcacac ggtgaagat tcggcaaat tcaacatca atgtcaatt tacaaatca gaattactc tttagtcca gtcaaagaa acaccaatg cctggcgct aatttcagt gttgattcc cttgattgt gacatgatg ttaacttgt caagccaac aaagacaac tacaccatt gcctttgaa ggctccaca ttgtattcg gaagatagt gactaccat gatgataag agcgagaat tccggtatg ggaactcat tgggttaag aaaccaaca aacaattgc aaattgacc gaagcttca atgactcag tccgattca agtagtttc accacttgg agcaagttg aagaaaaga agtagtgat aaccagtta aggcgtcac ccttctggt aataacaac aactcagga gacgaaact agtctaacg gaaaactcg gccaaaagc caaagtatg cccaatttg tacaaaaag aagttgaat tacctcaaa aaccacaat tttgtttcg accgaaacc attcgccga aaaccggtc aaagtgttt gatattcag caccaaagc agtagcgta tcagacatg gcgacgtcc atcgatgca agtacaagt gaaaccaca acaaacaaa catcaacac aacttcagt cttgttaaa ctcttcatg aagcaaaaa agtatgagt acggaaggt atgtcaaca acaatggat cagttatca tcgacggaa aactggcca ggaagtcaa tcaggtgaa agcaattcc tccgactcg aaaccagaa cttgaccaa aaaacgaac gaaagtagc gtcaggact tacgattta atcgcggaa gaaccagaa gaggaagtt tcacgaagc gaatcagtc gagcaatta tcggaaagt atatcgaaa attgactac agtgatgag aacgttaac aacaacaat accctcaaa cacgagttg atgaatcaa agcattcaa acgtcacag ttttccgaa attaccaac ttgaaaaat attgctaaa cctgccaca aaaccggtg aaaatcgtc gaaccttcg ttcctcaac aaactcaaa caagagggt gaggtgcaa aaaccagtc tatgtcctg taccccaac tacgttcta ccgaattta gactttttg aacaaacag gacgatatg actaaagtt ctactgatt ccgcaagaa ccaaatttg aaaactgtc aacaagcgg cgaccgttt agttgtacg gacttggaa atgttgaag aagaaaggt ttcagtcat gtgaaagac tgggattct ttaaatttc cttcttcca caagagtac cgcaaaatt ttggctgaa gtacccgaa atttccgaa aatattaaa aaatctcgg aaacgtcgg ccgtttagt tgcgattat tccagttct agttctagt actgcgacg caacctagt agcggttat cgggggtct tcgaccatg ttgagtgat tcccagaat agtcctgcc acaaatttg aatcccctt tttgtctat cgttatgac agcgtaaca agttcggaa gcaagtctt ttgaatagt ggtcaaagg tcaataatg accgctcca ccacttccc gtacgttgt gtgagtctc caacaaggc gaagtacca ccacgtcca ccgctccct aaaggaatt ttgcgaaaa gttgatgca aaaaggttc agtttgtac gaaacaagc gaggaagat ttgactgaa gaaggaatg tatcggaga aatattttg cggaagaaa cggttcagt gagactgaa gatgaaggt gtcgatgct ggaacatcg agtagtagt gtggacgag caccacgaa cctcccaaa acagatttc cttctctca aacataagt accgatgaa ctggtccaa ttggaggag tttctaaag ataagcgga atctcaacg tccgataac gaagaaatg accgacgaa agtctaatg cagttgcgt tcgtacgtc ggcaaattc ctctccttg aagatcaat caagacggt cctgggaaa aaaaccgta agtttcgcc gaaaaagtc aacgttttg cccaaacaa ttcacagcc ccaaacaac tcacccaat gtttcgaaa aactacttt gggaaaaac ctcgccgaa atgcccatc tgtgaagag agcgaaaat agtaactac agtccggtc cataaacca aatttggtg gaaaaacgc agtttagtg aacgctgta accgattct gtcgaacag ctcatcaat cacttttcg tcggcttgt aaccaatct gaattgaac actttgggc gattctgga gccactcca aatttggca aagttggca ctttcgaca ctgtgtcct gcactttac gctgtattg agtgatggt ttgaaacca agccttgag agcagtttc ggtgcaatt aacaattcc gtttggcaa gtggtggaa gcttcagct cagcaagga ccgctcact aaagctttg aacgaactg gtgatgcgg attaatagt gaggatgca ataacggaa ggtttggtt aagttcaat gctttcgtg ttcggttta cttaacgtg cgttcgttg gacgcgtgg gtgagttac ctaaaaacg agggaaagc atcttgagg aaacactac aataaggat tcgttgatt atggcacat gttggtggt ttgacttta cggacgtta ctcgattcg ctcatttct gccctgcaa cctttggcg ttgctcccg ttccagttg gatcttttg tttgagtac cgccagttg catttgagt ttcaaacgt ttggagcag ttgagccca actcataaa aaccggact tcgtcaagt tccgacgat gtgtccgtc gtgacggtg attcccgat cttttgagt tcgtccaaa aagaaagaa cgtccaagg tcgtgtgtc gagtccggt agtctccct aaaccaaac ttcaagctt ggtgatgtt acaacagct gcgaaaaaa cgctggtcg gggatttcc tcaaattcg aaactttac ctagtttac gatcgactg gcacgtgaa gatgaagaa gagtatact gatagtctt gaaaatcaa acaaaaccg gacgatggg gaaccacct gaatcactc gactcgaac atttccgac gataaaccg atcaacggc cgacgtttc aaaaagttg caacagaag tgggagatg ttaagtggg cgtgaatct aacccctcc cctccgtca tcgcccacc aataacaaa tcgaagatc cctcgtccc attacttcc ccaattaaa ccgtcgggg attcccatc ctgatttca ccatcaggt aagagtcaa aaaactccg aagaagaat ctagtccag gcaaagcca cccccaaaa cccaagaaa actactagt gtgaggacg agtagagtg gatcaagtc gagagtgag acaccaaga catgtccca agacccagt agtttgcct tataaggcc atgacggcg cagaaagtg gcaaaggtt acgccgcca cgaagggca gcatcgagt tcgttaagt aggaaaccg gtggggaaa agtaacgta ccaaaggtc gtgaggact ctatctcac cgcgtccct tccgaaagc gggcacttg tcgtataac gagggtgag agactcaag gttgtgtta gaagtggac gacaaatgg ttactctgt tgccgcgga tctcagaaa ggtctcgtc ccgaaatca gcagtgata tcagccgac agtcgtttc tga
- >TC016256 RA
agctcgagc gtttcagtt cgcttgttt cggtcgttg gtgttacaa gttgcgttt acttactgc gagtgtgat tattttatt gtgtttttt gtgccatgt gggatatat catcactct tgataacac cattggatc aaattgatt tttttgtga aaattgtgc attgacggg aatttttta ttatgttta ggagaacgg caagacaat gaacgaggt ggggagtct gggtattcg agggcttgc tgtcagccc agactctga ctgcaacag caaccacca cggctcagc aagttacct cagcaatac gtcttccca agatataga tttcattca agataactc tgattacca atggtttct agactatgg ctatcggga tggcggaac caatcatca cacgagtat tctgtcact tcccgagtc gtacgaagc caacgatat gaattatta tgacgctct ggcaaagaa catggacgc gaatttggc agaagccga tatggagag ttttcggac ggaggatat tcacgcact tttgactaa cttaccgcc aatgtgcac cgaccattt gagtcaaga aaataatcg gcaaggaga gagttatgc aagtgtctc gggttcaat gatggcaaa atttgattt cgacagctc gttaagtcc tcatagtag ttcacacgg tgaagattc ggcaaattc aacatcaat gtcaattta caaatcaga attactctt tagtccagt caaagaaac accaatgcc tggcgctaa tttcagtgt tgattccct tgattgtga catgatgtt aacttgtca agccaacaa agacaacta caccattgc ctttgaagg ctccacatt gtattcgga agatagtga ctaccatga tgataagag cgagaattc cggtatggg aactcattg ggttaagaa accaacaaa caattgcaa attgaccga agcttcaat gactcagtc cgattcaag tagtttcac cacttggag caagttgaa gaaaagaag tagtgataa ccagttaag gcgtcaccc ttctggtaa taacaacaa ctcaggaga cgaaactag tctaacgga aaactcggc caaaagcca aagtatgcc caatttgta caaaaagaa gttgaatta cctcaaaaa ccacaattt tgtttcgac cgaaaccat tcgccgaaa accggtcaa agtgtttga tattcagca ccaaagcag tagcgtatc agacatggc gacgtccat cgatgcaag tacaagtga aaccacaac aaacaaaca tcaacacaa cttcagtct tgttaaact cttcatgaa gcaaaaaag tatgagtac ggaaggtat gtcaacaac aatggatca gttatcatc gacggaaaa ctggccagg aagtcaatc aggtgaaag caattcctc cgactcgaa accagaact tgaccaaaa aacgaacga aagtagcgt caggactta cgatttaat cgcggaaga accagaaga ggaagtttc acgaagcga atcagtcga gcaattatc ggaaagtat atcgaaaat tgactacag tgatgagaa cgttaacaa caacaatac cctcaaaca cgagttgat gaatcaaag cattcaaac gtcacagtt ttccgaaat taccaactt gaaaaatat tgctaaacc tgccacaaa accggtgaa aatcgtcga accttcgtt cctcaacaa actcaaaca agagggtga ggtgcaaaa accagtcta tgtcctgta ccccaacta cgttctacc gaatttaga ctttttgaa caaacagga cgatatgac taaagttct actgattcc gcaagaacc aaatttgaa aactgtcaa caagcggcg accgtttag ttgtacgga cttggaaat gttgaagaa gaaaggttt cagtcatgt gaaagactg ggattcttt aaatttcct tcttccaca agagtaccg caaaatttt ggctgaagt acccgaaat ttccgaaaa tattaaaaa atctcggaa acgtcggcc gtttagttg cgattattc cagttctag ttctagtac tgcgacgca acctagtag cggttatcg ggggtcttc gaccatgtt gagtgattc ccagaatag tcctgccac aaatttgaa tcccctttt tgtctatcg ttatgacag cgtaacaag ttcggaagc aagtctttt gaatagtgg tcaaaggtc aataatgac cgctccacc acttcccgt acgttgtgt gagtctcca acaaggcga agtaccacc acgtccacc gctccctaa aggaatttt gcgaaaagt tgatgcaaa aaggttcag tttgtacga aacaagcga ggaagattt gactgaaga aggaatgta tcggagaaa tattttgcg gaagaaacg gttcagtga gactgaaga tgaaggtgt cgatgctgg aacatcgag tagtagtgt ggacgagca ccacgaacc tcccaaaac agatttcct tctctcaaa cataagtac cgatgaact ggtccaatt ggaggagtt tctaaagat aagcggaat ctcaacgtc cgataacga agaaatgac cgacgaaag tctaatgca gttgcgttc gtacgtcgg caaattcct ctccttgaa gatcaatca agacggtcc tgggaaaaa aaccgtaag tttcgccga aaaagtcaa cgttttgcc caaacaatt cacagcccc aaacaactc acccaatgt ttcgaaaaa ctactttgg gaaaaacct cgccgaaat gcccatctg tgaagagag cgaaaatag taactacag tccggtcca taaaccaaa tttggtgga aaaacgcag tttagtgaa cgctgtaac cgattctgt cgaacagct catcaatca cttttcgtc ggcttgtaa ccaatctga attgaacac tttgggcga ttctggagc cactccaaa tttggcaaa gttggcact ttcgacact gtgtcctgc actttacgc tgtattgag tgatggttt gaaaccaag ccttgagag cagtttcgg tgcaattaa caattccgt ttggcaagt ggtggaagc ttcagctca gcaaggacc gctcactaa agctttgaa cgaactggt gatgcggat taatagtga ggatgcaat aacggaagg tttggttaa gttcaatgc tttcgtgtt cggtttact taacgtgcg ttcgttgga cgcgtgggt gagttacct aaaaacgag ggaaagcat cttgaggaa acactacaa taaggattc gttgattat ggcacatgt tggtggttt gactttacg gacgttact cgattcgct catttctgc cctgcaacc tttggcgtt gctcccgtt ccagttgga tcttttgtt tgagtaccg ccagttgca tttgagttt caaacgttt ggagcagtt gagcccaac tcataaaaa ccggacttc gtcaagttc cgacgatgt gtccgtcgt gacggtgat tcccgatct tttgagttc gtccaaaaa gaaagaacg tccaaggtc gtgtgtcga gtccggtag tctccctaa accaaactt caagcttgg tgatgttac aacagctgc gaaaaaacg ctggtcggg gatttcctc aaattcgaa actttacct agtttacga tcgactggc acgtgaaga tgaagaaga gtatactga tagtcttga aaatcaaac aaaaccgga cgatgggga accacctga atcactcga ctcgaacat ttccgacga taaaccgat caacggccg acgtttcaa aaagttgca acagaagtg ggagatgtt aagtgggcg tgaatctaa cccctcccc tccgtcatc gcccaccaa taacaaatc gaagatccc tcgtcccat tacttcccc aattaaacc gtcggggat tcccatcct gatttcacc atcaggtaa gagtcaaaa aactccgaa gaagaatct agtccaggc aaagccacc cccaaaacc caagaaaac tactagtgt gaggacgag tagagtgga tcaagtcga gagtgagac accaagaca tgtcccaag acccagtag tttgcctta taaggccat gacggcgca gaaagtggc aaaggttac gccgccacg aagggcagc atcgagttc gttaagtag gaaaccggt ggggaaaag taacgtacc aaaggtcgt gaggactct atctcaccg cgtcccttc cgaaagcgg gcacttgtc gtataacga gggtgagag actcaaggt tgtgttaga agtggacga caaatggtt actctgttg ccgcggatc tcagaaagg tctcgtccc gaaatcagc agtgatatc agccgacag tcgtttctg aaattccct acaattgta aatatacgt agtgttttt tcagttgta catttttat acagactgg atttgtgat tttagtata ttttaagtc aagacacgt atattatta ttgttaaaa aaagtatta atcatattg ttgcttgtt gtaacagca aatttgata ttatttatt attgcttgt aggatttgt cattatgta cttaatagg ggcgcgact caattgtta aatcttgca cgtcatgaa atttttgca catatctta ggtggttgg ttgggttct tgtttgctc ttcattttt tggtgctgt tttattgtt tttaacact cacaccttc gcaccgttc cttgcatgc taacatgtg attccccca attttgcag tacctacta aaattggca tcactgcga gctaattta gctcaaaag tgataattt tgcactcgc ttcgttcga ttctagtct ttaaagcac cacagcatg agtggtccg agtattttc gacaatacc tccaggatt caacccagc tcatctcta attgatcac tttgtgacg tgcaagaaa gccgtttcg tttcctttt tgcttctgt aatgtgttg ctagatgta atagtgtag tttgaaaaa tcaaagttt gtgaacggg gccttgagg gatttttct ggtcctagt tttcgtatg cttgcttta tttgcgtcc actatgtgt atatattga accgaacgc ttttgtgat gtatataat gttaccaaa ggatcgaac gatcgatcg atttcagcg atattctaa gtttctttt ttcattttt ttgggaaca atttttgtg ttgtaacct caccatagg tgccataaa cacccgttt tgttttgta ttttcattg atttttgtc gaaattgta gctagagac tatggaaat agtgtaggg attaaaatc atgtaactc agtcataat aaatgaatt ataatacat tttgttggt ttctttatt aaaatacac cgaaaactt tcttcattg aatagttta tgtcgactt tagagtccg tttgccata agagtaaca cgtaagttg atgcattgc cgcaaatct ggaagaaat ggaatcaaa acagataaa agataaaag aggtgagta aatataata ccgatataa aaattggta agtcgatca aaaacatgt tgcctgcgt aaatctcaa acggttcgt gcaatagtt gaagaccaa aggcattta gctgaaaca aataaatac taggggaag gc
