Status
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Gene information
General information
TC016256 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 259002..303305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016256
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05982

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016256 RA
      MNEVGSLGI RGLAVSPDS DCNSNHHGS ASYLSNTSS 
      QDIDFIQDN SDYQWFLDY GYRDGGTNH HTSILSLPE 
      SYEANDMNY YDALAKNMD ANLAEADME SFRTEDIHA 
      LLTNLPPMC TDHLSQENN RQGESYASV SGSMMAKFD 
      FDSSLSPHS SSHGEDSAN STSMSIYKS ELLFSPVKE 
      TPMPGANFS VDSLDCDMM LTCQANKDN YTIAFEGST 
      LYSEDSDYH DDKSENSGM GTHWVKKPT NNCKLTEAS 
      MTQSDSSSF TTWSKLKKR SSDNQLRRH PSGNNNNSG 
      DETSLTENS AKSQSMPNL YKKKLNYLK NHNFVSTET 
      IRRKPVKVF DIQHQSSSV SDMATSIDA STSETTTNK 
      HQHNFSLVK LFMKQKSMS TEGMSTTMD QLSSTENWP 
      GSQSGESNS SDSKPELDQ KTNESSVRT YDLIAEEPE 
      EEVSRSESV EQLSESISK IDYSDENVN NNNTLKHEL 
      MNQSIQTSQ FSEITNLKN IAKPATKPV KIVEPSFLN 
      KLKQEGEVQ KPVYVLYPN YVLPNLDFL NKQDDMTKV 
      LLIPQEPNL KTVNKRRPF SCTDLEMLK KKGFSHVKD 
      WDSLNFLLP QEYRKILAE VPEISENIK KSRKRRPFS 
      CDYSSSSSS TATQPSSGY RGSSTMLSD SQNSPATNL 
      NPLFVYRYD SVTSSEASL LNSGQRSIM TAPPLPVRC 
      VSLQQGEVP PRPPLPKGI LRKVDAKRF SLYETSEED 
      LTEEGMYRR NILRKKRFS ETEDEGVDA GTSSSSVDE 
      HHEPPKTDF LLSNISTDE LVQLEEFLK ISGISTSDN 
      EEMTDESLM QLRSYVGKF LSLKINQDG PGKKTVSFA 
      EKVNVLPKQ FTAPNNSPN VSKNYFGKN LAEMPICEE 
      SENSNYSPV HKPNLVEKR SLVNAVTDS VEQLINHFS 
      SACNQSELN TLGDSGATP NLAKLALST LCPALYAVL 
      SDGLKPSLE SSFGAINNS VWQVVEASA QQGPLTKAL 
      NELVMRINS EDAITEGLV KFNAFVFGL LNVRSLDAW 
      VSYLKTRES ILRKHYNKD SLIMAHVGG LTLRTLLDS 
      LISALQPLA LLPFQLDLL FEYRQLHLS FKRLEQLSP 
      THKNRTSSS SDDVSVVTV IPDLLSSSK KKERPRSCV 
      ESGSLPKPN FKLGDVTTA AKKRWSGIS SNSKLYLVY 
      DRLAREDEE EYTDSLENQ TKPDDGEPP ESLDSNISD 
      DKPINGRRF KKLQQKWEM LSGRESNPS PPSSPTNNK 
      SKIPRPITS PIKPSGIPI LISPSGKSQ KTPKKNLVQ 
      AKPPPKPKK TTSVRTSRV DQVESETPR HVPRPSSLP 
      YKAMTAQKV AKVTPPRRA ASSSLSRKP VGKSNVPKV 
      VRTLSHRVP SESGHLSYN EGERLKVVL EVDDKWLLC 
      CRGSQKGLV PKSAVISAD SRF
    CDS sequence
    • >TC016256 RA
      atgaacgag gtggggagt ctgggtatt cgagggctt 
      gctgtcagc ccagactct gactgcaac agcaaccac 
      cacggctca gcaagttac ctcagcaat acgtcttcc 
      caagatata gatttcatt caagataac tctgattac 
      caatggttt ctagactat ggctatcgg gatggcgga 
      accaatcat cacacgagt attctgtca cttcccgag 
      tcgtacgaa gccaacgat atgaattat tatgacgct 
      ctggcaaag aacatggac gcgaatttg gcagaagcc 
      gatatggag agttttcgg acggaggat attcacgca 
      cttttgact aacttaccg ccaatgtgc accgaccat 
      ttgagtcaa gaaaataat cggcaagga gagagttat 
      gcaagtgtc tcgggttca atgatggca aaatttgat 
      ttcgacagc tcgttaagt cctcatagt agttcacac 
      ggtgaagat tcggcaaat tcaacatca atgtcaatt 
      tacaaatca gaattactc tttagtcca gtcaaagaa 
      acaccaatg cctggcgct aatttcagt gttgattcc 
      cttgattgt gacatgatg ttaacttgt caagccaac 
      aaagacaac tacaccatt gcctttgaa ggctccaca 
      ttgtattcg gaagatagt gactaccat gatgataag 
      agcgagaat tccggtatg ggaactcat tgggttaag 
      aaaccaaca aacaattgc aaattgacc gaagcttca 
      atgactcag tccgattca agtagtttc accacttgg 
      agcaagttg aagaaaaga agtagtgat aaccagtta 
      aggcgtcac ccttctggt aataacaac aactcagga 
      gacgaaact agtctaacg gaaaactcg gccaaaagc 
      caaagtatg cccaatttg tacaaaaag aagttgaat 
      tacctcaaa aaccacaat tttgtttcg accgaaacc 
      attcgccga aaaccggtc aaagtgttt gatattcag 
      caccaaagc agtagcgta tcagacatg gcgacgtcc 
      atcgatgca agtacaagt gaaaccaca acaaacaaa 
      catcaacac aacttcagt cttgttaaa ctcttcatg 
      aagcaaaaa agtatgagt acggaaggt atgtcaaca 
      acaatggat cagttatca tcgacggaa aactggcca 
      ggaagtcaa tcaggtgaa agcaattcc tccgactcg 
      aaaccagaa cttgaccaa aaaacgaac gaaagtagc 
      gtcaggact tacgattta atcgcggaa gaaccagaa 
      gaggaagtt tcacgaagc gaatcagtc gagcaatta 
      tcggaaagt atatcgaaa attgactac agtgatgag 
      aacgttaac aacaacaat accctcaaa cacgagttg 
      atgaatcaa agcattcaa acgtcacag ttttccgaa 
      attaccaac ttgaaaaat attgctaaa cctgccaca 
      aaaccggtg aaaatcgtc gaaccttcg ttcctcaac 
      aaactcaaa caagagggt gaggtgcaa aaaccagtc 
      tatgtcctg taccccaac tacgttcta ccgaattta 
      gactttttg aacaaacag gacgatatg actaaagtt 
      ctactgatt ccgcaagaa ccaaatttg aaaactgtc 
      aacaagcgg cgaccgttt agttgtacg gacttggaa 
      atgttgaag aagaaaggt ttcagtcat gtgaaagac 
      tgggattct ttaaatttc cttcttcca caagagtac 
      cgcaaaatt ttggctgaa gtacccgaa atttccgaa 
      aatattaaa aaatctcgg aaacgtcgg ccgtttagt 
      tgcgattat tccagttct agttctagt actgcgacg 
      caacctagt agcggttat cgggggtct tcgaccatg 
      ttgagtgat tcccagaat agtcctgcc acaaatttg 
      aatcccctt tttgtctat cgttatgac agcgtaaca 
      agttcggaa gcaagtctt ttgaatagt ggtcaaagg 
      tcaataatg accgctcca ccacttccc gtacgttgt 
      gtgagtctc caacaaggc gaagtacca ccacgtcca 
      ccgctccct aaaggaatt ttgcgaaaa gttgatgca 
      aaaaggttc agtttgtac gaaacaagc gaggaagat 
      ttgactgaa gaaggaatg tatcggaga aatattttg 
      cggaagaaa cggttcagt gagactgaa gatgaaggt 
      gtcgatgct ggaacatcg agtagtagt gtggacgag 
      caccacgaa cctcccaaa acagatttc cttctctca 
      aacataagt accgatgaa ctggtccaa ttggaggag 
      tttctaaag ataagcgga atctcaacg tccgataac 
      gaagaaatg accgacgaa agtctaatg cagttgcgt 
      tcgtacgtc ggcaaattc ctctccttg aagatcaat 
      caagacggt cctgggaaa aaaaccgta agtttcgcc 
      gaaaaagtc aacgttttg cccaaacaa ttcacagcc 
      ccaaacaac tcacccaat gtttcgaaa aactacttt 
      gggaaaaac ctcgccgaa atgcccatc tgtgaagag 
      agcgaaaat agtaactac agtccggtc cataaacca 
      aatttggtg gaaaaacgc agtttagtg aacgctgta 
      accgattct gtcgaacag ctcatcaat cacttttcg 
      tcggcttgt aaccaatct gaattgaac actttgggc 
      gattctgga gccactcca aatttggca aagttggca 
      ctttcgaca ctgtgtcct gcactttac gctgtattg 
      agtgatggt ttgaaacca agccttgag agcagtttc 
      ggtgcaatt aacaattcc gtttggcaa gtggtggaa 
      gcttcagct cagcaagga ccgctcact aaagctttg 
      aacgaactg gtgatgcgg attaatagt gaggatgca 
      ataacggaa ggtttggtt aagttcaat gctttcgtg 
      ttcggttta cttaacgtg cgttcgttg gacgcgtgg 
      gtgagttac ctaaaaacg agggaaagc atcttgagg 
      aaacactac aataaggat tcgttgatt atggcacat 
      gttggtggt ttgacttta cggacgtta ctcgattcg 
      ctcatttct gccctgcaa cctttggcg ttgctcccg 
      ttccagttg gatcttttg tttgagtac cgccagttg 
      catttgagt ttcaaacgt ttggagcag ttgagccca 
      actcataaa aaccggact tcgtcaagt tccgacgat 
      gtgtccgtc gtgacggtg attcccgat cttttgagt 
      tcgtccaaa aagaaagaa cgtccaagg tcgtgtgtc 
      gagtccggt agtctccct aaaccaaac ttcaagctt 
      ggtgatgtt acaacagct gcgaaaaaa cgctggtcg 
      gggatttcc tcaaattcg aaactttac ctagtttac 
      gatcgactg gcacgtgaa gatgaagaa gagtatact 
      gatagtctt gaaaatcaa acaaaaccg gacgatggg 
      gaaccacct gaatcactc gactcgaac atttccgac 
      gataaaccg atcaacggc cgacgtttc aaaaagttg 
      caacagaag tgggagatg ttaagtggg cgtgaatct 
      aacccctcc cctccgtca tcgcccacc aataacaaa 
      tcgaagatc cctcgtccc attacttcc ccaattaaa 
      ccgtcgggg attcccatc ctgatttca ccatcaggt 
      aagagtcaa aaaactccg aagaagaat ctagtccag 
      gcaaagcca cccccaaaa cccaagaaa actactagt 
      gtgaggacg agtagagtg gatcaagtc gagagtgag 
      acaccaaga catgtccca agacccagt agtttgcct 
      tataaggcc atgacggcg cagaaagtg gcaaaggtt 
      acgccgcca cgaagggca gcatcgagt tcgttaagt 
      aggaaaccg gtggggaaa agtaacgta ccaaaggtc 
      gtgaggact ctatctcac cgcgtccct tccgaaagc 
      gggcacttg tcgtataac gagggtgag agactcaag 
      gttgtgtta gaagtggac gacaaatgg ttactctgt 
      tgccgcgga tctcagaaa ggtctcgtc ccgaaatca 
      gcagtgata tcagccgac agtcgtttc tga
    mRNA sequence
    • >TC016256 RA
      agctcgagc gtttcagtt cgcttgttt cggtcgttg 
      gtgttacaa gttgcgttt acttactgc gagtgtgat 
      tattttatt gtgtttttt gtgccatgt gggatatat 
      catcactct tgataacac cattggatc aaattgatt 
      tttttgtga aaattgtgc attgacggg aatttttta 
      ttatgttta ggagaacgg caagacaat gaacgaggt 
      ggggagtct gggtattcg agggcttgc tgtcagccc 
      agactctga ctgcaacag caaccacca cggctcagc 
      aagttacct cagcaatac gtcttccca agatataga 
      tttcattca agataactc tgattacca atggtttct 
      agactatgg ctatcggga tggcggaac caatcatca 
      cacgagtat tctgtcact tcccgagtc gtacgaagc 
      caacgatat gaattatta tgacgctct ggcaaagaa 
      catggacgc gaatttggc agaagccga tatggagag 
      ttttcggac ggaggatat tcacgcact tttgactaa 
      cttaccgcc aatgtgcac cgaccattt gagtcaaga 
      aaataatcg gcaaggaga gagttatgc aagtgtctc 
      gggttcaat gatggcaaa atttgattt cgacagctc 
      gttaagtcc tcatagtag ttcacacgg tgaagattc 
      ggcaaattc aacatcaat gtcaattta caaatcaga 
      attactctt tagtccagt caaagaaac accaatgcc 
      tggcgctaa tttcagtgt tgattccct tgattgtga 
      catgatgtt aacttgtca agccaacaa agacaacta 
      caccattgc ctttgaagg ctccacatt gtattcgga 
      agatagtga ctaccatga tgataagag cgagaattc 
      cggtatggg aactcattg ggttaagaa accaacaaa 
      caattgcaa attgaccga agcttcaat gactcagtc 
      cgattcaag tagtttcac cacttggag caagttgaa 
      gaaaagaag tagtgataa ccagttaag gcgtcaccc 
      ttctggtaa taacaacaa ctcaggaga cgaaactag 
      tctaacgga aaactcggc caaaagcca aagtatgcc 
      caatttgta caaaaagaa gttgaatta cctcaaaaa 
      ccacaattt tgtttcgac cgaaaccat tcgccgaaa 
      accggtcaa agtgtttga tattcagca ccaaagcag 
      tagcgtatc agacatggc gacgtccat cgatgcaag 
      tacaagtga aaccacaac aaacaaaca tcaacacaa 
      cttcagtct tgttaaact cttcatgaa gcaaaaaag 
      tatgagtac ggaaggtat gtcaacaac aatggatca 
      gttatcatc gacggaaaa ctggccagg aagtcaatc 
      aggtgaaag caattcctc cgactcgaa accagaact 
      tgaccaaaa aacgaacga aagtagcgt caggactta 
      cgatttaat cgcggaaga accagaaga ggaagtttc 
      acgaagcga atcagtcga gcaattatc ggaaagtat 
      atcgaaaat tgactacag tgatgagaa cgttaacaa 
      caacaatac cctcaaaca cgagttgat gaatcaaag 
      cattcaaac gtcacagtt ttccgaaat taccaactt 
      gaaaaatat tgctaaacc tgccacaaa accggtgaa 
      aatcgtcga accttcgtt cctcaacaa actcaaaca 
      agagggtga ggtgcaaaa accagtcta tgtcctgta 
      ccccaacta cgttctacc gaatttaga ctttttgaa 
      caaacagga cgatatgac taaagttct actgattcc 
      gcaagaacc aaatttgaa aactgtcaa caagcggcg 
      accgtttag ttgtacgga cttggaaat gttgaagaa 
      gaaaggttt cagtcatgt gaaagactg ggattcttt 
      aaatttcct tcttccaca agagtaccg caaaatttt 
      ggctgaagt acccgaaat ttccgaaaa tattaaaaa 
      atctcggaa acgtcggcc gtttagttg cgattattc 
      cagttctag ttctagtac tgcgacgca acctagtag 
      cggttatcg ggggtcttc gaccatgtt gagtgattc 
      ccagaatag tcctgccac aaatttgaa tcccctttt 
      tgtctatcg ttatgacag cgtaacaag ttcggaagc 
      aagtctttt gaatagtgg tcaaaggtc aataatgac 
      cgctccacc acttcccgt acgttgtgt gagtctcca 
      acaaggcga agtaccacc acgtccacc gctccctaa 
      aggaatttt gcgaaaagt tgatgcaaa aaggttcag 
      tttgtacga aacaagcga ggaagattt gactgaaga 
      aggaatgta tcggagaaa tattttgcg gaagaaacg 
      gttcagtga gactgaaga tgaaggtgt cgatgctgg 
      aacatcgag tagtagtgt ggacgagca ccacgaacc 
      tcccaaaac agatttcct tctctcaaa cataagtac 
      cgatgaact ggtccaatt ggaggagtt tctaaagat 
      aagcggaat ctcaacgtc cgataacga agaaatgac 
      cgacgaaag tctaatgca gttgcgttc gtacgtcgg 
      caaattcct ctccttgaa gatcaatca agacggtcc 
      tgggaaaaa aaccgtaag tttcgccga aaaagtcaa 
      cgttttgcc caaacaatt cacagcccc aaacaactc 
      acccaatgt ttcgaaaaa ctactttgg gaaaaacct 
      cgccgaaat gcccatctg tgaagagag cgaaaatag 
      taactacag tccggtcca taaaccaaa tttggtgga 
      aaaacgcag tttagtgaa cgctgtaac cgattctgt 
      cgaacagct catcaatca cttttcgtc ggcttgtaa 
      ccaatctga attgaacac tttgggcga ttctggagc 
      cactccaaa tttggcaaa gttggcact ttcgacact 
      gtgtcctgc actttacgc tgtattgag tgatggttt 
      gaaaccaag ccttgagag cagtttcgg tgcaattaa 
      caattccgt ttggcaagt ggtggaagc ttcagctca 
      gcaaggacc gctcactaa agctttgaa cgaactggt 
      gatgcggat taatagtga ggatgcaat aacggaagg 
      tttggttaa gttcaatgc tttcgtgtt cggtttact 
      taacgtgcg ttcgttgga cgcgtgggt gagttacct 
      aaaaacgag ggaaagcat cttgaggaa acactacaa 
      taaggattc gttgattat ggcacatgt tggtggttt 
      gactttacg gacgttact cgattcgct catttctgc 
      cctgcaacc tttggcgtt gctcccgtt ccagttgga 
      tcttttgtt tgagtaccg ccagttgca tttgagttt 
      caaacgttt ggagcagtt gagcccaac tcataaaaa 
      ccggacttc gtcaagttc cgacgatgt gtccgtcgt 
      gacggtgat tcccgatct tttgagttc gtccaaaaa 
      gaaagaacg tccaaggtc gtgtgtcga gtccggtag 
      tctccctaa accaaactt caagcttgg tgatgttac 
      aacagctgc gaaaaaacg ctggtcggg gatttcctc 
      aaattcgaa actttacct agtttacga tcgactggc 
      acgtgaaga tgaagaaga gtatactga tagtcttga 
      aaatcaaac aaaaccgga cgatgggga accacctga 
      atcactcga ctcgaacat ttccgacga taaaccgat 
      caacggccg acgtttcaa aaagttgca acagaagtg 
      ggagatgtt aagtgggcg tgaatctaa cccctcccc 
      tccgtcatc gcccaccaa taacaaatc gaagatccc 
      tcgtcccat tacttcccc aattaaacc gtcggggat 
      tcccatcct gatttcacc atcaggtaa gagtcaaaa 
      aactccgaa gaagaatct agtccaggc aaagccacc 
      cccaaaacc caagaaaac tactagtgt gaggacgag 
      tagagtgga tcaagtcga gagtgagac accaagaca 
      tgtcccaag acccagtag tttgcctta taaggccat 
      gacggcgca gaaagtggc aaaggttac gccgccacg 
      aagggcagc atcgagttc gttaagtag gaaaccggt 
      ggggaaaag taacgtacc aaaggtcgt gaggactct 
      atctcaccg cgtcccttc cgaaagcgg gcacttgtc 
      gtataacga gggtgagag actcaaggt tgtgttaga 
      agtggacga caaatggtt actctgttg ccgcggatc 
      tcagaaagg tctcgtccc gaaatcagc agtgatatc 
      agccgacag tcgtttctg aaattccct acaattgta 
      aatatacgt agtgttttt tcagttgta catttttat 
      acagactgg atttgtgat tttagtata ttttaagtc 
      aagacacgt atattatta ttgttaaaa aaagtatta 
      atcatattg ttgcttgtt gtaacagca aatttgata 
      ttatttatt attgcttgt aggatttgt cattatgta 
      cttaatagg ggcgcgact caattgtta aatcttgca 
      cgtcatgaa atttttgca catatctta ggtggttgg 
      ttgggttct tgtttgctc ttcattttt tggtgctgt 
      tttattgtt tttaacact cacaccttc gcaccgttc 
      cttgcatgc taacatgtg attccccca attttgcag 
      tacctacta aaattggca tcactgcga gctaattta 
      gctcaaaag tgataattt tgcactcgc ttcgttcga 
      ttctagtct ttaaagcac cacagcatg agtggtccg 
      agtattttc gacaatacc tccaggatt caacccagc 
      tcatctcta attgatcac tttgtgacg tgcaagaaa 
      gccgtttcg tttcctttt tgcttctgt aatgtgttg 
      ctagatgta atagtgtag tttgaaaaa tcaaagttt 
      gtgaacggg gccttgagg gatttttct ggtcctagt 
      tttcgtatg cttgcttta tttgcgtcc actatgtgt 
      atatattga accgaacgc ttttgtgat gtatataat 
      gttaccaaa ggatcgaac gatcgatcg atttcagcg 
      atattctaa gtttctttt ttcattttt ttgggaaca 
      atttttgtg ttgtaacct caccatagg tgccataaa 
      cacccgttt tgttttgta ttttcattg atttttgtc 
      gaaattgta gctagagac tatggaaat agtgtaggg 
      attaaaatc atgtaactc agtcataat aaatgaatt 
      ataatacat tttgttggt ttctttatt aaaatacac 
      cgaaaactt tcttcattg aatagttta tgtcgactt 
      tagagtccg tttgccata agagtaaca cgtaagttg 
      atgcattgc cgcaaatct ggaagaaat ggaatcaaa 
      acagataaa agataaaag aggtgagta aatataata 
      ccgatataa aaattggta agtcgatca aaaacatgt 
      tgcctgcgt aaatctcaa acggttcgt gcaatagtt 
      gaagaccaa aggcattta gctgaaaca aataaatac 
      taggggaag gc
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