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Gene information
General information
TC016251 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 328977..333600
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016251
    iBeetle screen iB_05981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva dorsal irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva ventral irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment random partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016251 RA
      MKCIQLFVF FSLKIGVDL SDPLVELPN GLIRGQRIK 
      TQNNEFYSF KKIPYAQPP IGTLRFQAP REPRNWDGV 
      LDCTTMDVV CYQMFNKLE NQSENCLFL NVYTPNLPE 
      FVNESFPVM VYIHGGGFT EGMTQQYDP RLFVENGVV 
      LVTFNYRLG PFGFLSTQD DTIPGNNGL KDQNMALKW 
      VHKNIEFFS GDPQKVTLF GHSAGSASV GYHLLNLKS 
      EDLFWAAIC QSGSVLGPW AYQRQPRRQ AFLTASFIN 
      ETFLEQNDS KKLLEFLQN VEPGTIRSA AEMFYDFIL 
      NGTSDDFAA TEGSSWAPT LEIKNEDSF ISEKMFKLL 
      KSGKFVKVP LMIGMTTEE NLSMDHKNF DKNVEQYDI 
      SYKTLVPVD ITGDEEKLN EIGRKIRDM YTGGKNFSK 
      NIGDAIRFS SDNDMTRPI MKQAEIYSK FAPTYFYQF 
      SYDGNFAGF SLTYPGTGS VAHAEELYY LFDFIHDIK 
      SFPKEDQIV RNRLIKLWT NFAKFSFLL GIRHQR
    • >TC016251 RB
      MKCIQLFVF FSLKIGVDL SDPLVELPN GLIRGQRIK 
      TQNNEFYSF KKIPYAQPP IGTLRFQAP REPRNWDGV 
      LDCTTMDVV CYQMFNKLE NQSENCLFL NVYTPNLPE 
      FVNESFPVM VYIHGGGFT EGMTQQYDP RLFVENGVV 
      LVTFNYRLG PFGFLSTQD DTIPGNNGL KDQNMALKW 
      VHKNIEFFS GDPQKVTLF GHSAGSASV GYHLLNLKS 
      EDLFWAAIC QSGSVLGPW AYQRQPRRQ AFLTASFIN 
      ETFLEQNDS KKLLEFLQN VEPGTIRSA AEMFYDFIL 
      NGTSDDFAA TEGSSWAPT LEIKNEDSF ISEKMFKLL 
      KSGKFVKVP LMIGMTTEE NLSMDHKNF DKNVEQYDI 
      SYKTLVPVD ITGDEEKLN EIGRKIRDM YTGGKNFSK 
      NIGDAIRFS SDNDMTRPI MKQAEIYSK FAPTYFYQF 
      SYDGNFAGF SLTYPGTGS VAHAEELYY LFDFIHDIK 
      SFPKEDQIV RNRLIKLWT NFAKFRNPT PTLMENVSW 
      PQLKVEEND FPYLDIGAN LTVKNFPKK EIYKKWSQI 
      YESLGHDDF DTY
    CDS sequence
    • >TC016251 RA
      atgaagtgt attcaacta tttgtgttt ttctcacta 
      aaaattggg gtcgatctg agtgaccct ttggtggaa 
      ctcccaaac ggcttaata agagggcaa agaataaaa 
      acccaaaac aatgaattc tactctttc aaaaaaata 
      ccatatgcc caaccacct attggtact ttgcgattt 
      caagcccct cgtgaaccc aggaattgg gacggtgtt 
      ttggactgc accactatg gacgttgtt tgttaccag 
      atgtttaac aaattggag aatcaatca gaaaattgc 
      cttttttta aatgtttac acacccaac ttgccggag 
      tttgtaaat gagtcattt ccagtcatg gtatacatc 
      catggtggt ggtttcaca gaaggaatg acccaacaa 
      tacgaccca cggctcttt gtggaaaat ggggtggtt 
      ttagttact tttaactac cgtttagga ccatttggt 
      tttctctcc actcaagat gatacgatc cccggaaac 
      aacgggtta aaagatcag aatatggcc ttgaaatgg 
      gtgcacaaa aatattgaa tttttcagt ggggaccct 
      caaaaagtc acacttttt gggcacagt gcagggtca 
      gcttcagtc ggttatcac ctccttaac ctcaaatcg 
      gaagattta ttttgggcc gcaatttgt caaagtggg 
      tccgtttta gggccatgg gcctatcaa aggcaaccg 
      agacgacaa gctttcctc actgctagt tttataaat 
      gagacattt cttgagcaa aatgattcg aaaaaattg 
      cttgaattt ttgcaaaac gtggaacca ggaacgatt 
      cgatcagct gccgaaatg ttttacgat tttattttg 
      aatggtaca agtgatgat tttgcggca actgaagga 
      tcaagttgg gccccgact ttggaaatc aaaaatgag 
      gattctttt attagtgaa aaaatgttc aaattgttg 
      aaaagtggt aaatttgtt aaagtgcct ttaatgatt 
      gggatgacc actgaggaa aatttatca atggaccac 
      aaaaatttt gacaaaaat gtcgaacag tatgatatt 
      tcttataaa actttggtg ccggttgac ataacagga 
      gatgaagaa aaattaaat gaaattggt cgaaagatt 
      cgagacatg tacacagga gggaaaaac ttttcgaaa 
      aatattgga gacgccatt cgattttcc agtgataat 
      gacatgact cgcccgatt atgaaacaa gccgagatt 
      tactcaaaa ttcgctcca acttacttt taccaattt 
      tcttacgac ggaaatttt gcagggttt agtctaact 
      tatccaggt actggaagt gttgctcat gccgaggaa 
      ttatattat ctttttgat tttattcat gacatcaaa 
      agttttcca aaagaagat caaattgta cgaaatcgt 
      ttaattaaa ctgtggacc aattttgcc aaatttagt 
      tttctttta ggaatccga caccaacgc taa
    • >TC016251 RB
      atgaagtgt attcaacta tttgtgttt ttctcacta 
      aaaattggg gtcgatctg agtgaccct ttggtggaa 
      ctcccaaac ggcttaata agagggcaa agaataaaa 
      acccaaaac aatgaattc tactctttc aaaaaaata 
      ccatatgcc caaccacct attggtact ttgcgattt 
      caagcccct cgtgaaccc aggaattgg gacggtgtt 
      ttggactgc accactatg gacgttgtt tgttaccag 
      atgtttaac aaattggag aatcaatca gaaaattgc 
      cttttttta aatgtttac acacccaac ttgccggag 
      tttgtaaat gagtcattt ccagtcatg gtatacatc 
      catggtggt ggtttcaca gaaggaatg acccaacaa 
      tacgaccca cggctcttt gtggaaaat ggggtggtt 
      ttagttact tttaactac cgtttagga ccatttggt 
      tttctctcc actcaagat gatacgatc cccggaaac 
      aacgggtta aaagatcag aatatggcc ttgaaatgg 
      gtgcacaaa aatattgaa tttttcagt ggggaccct 
      caaaaagtc acacttttt gggcacagt gcagggtca 
      gcttcagtc ggttatcac ctccttaac ctcaaatcg 
      gaagattta ttttgggcc gcaatttgt caaagtggg 
      tccgtttta gggccatgg gcctatcaa aggcaaccg 
      agacgacaa gctttcctc actgctagt tttataaat 
      gagacattt cttgagcaa aatgattcg aaaaaattg 
      cttgaattt ttgcaaaac gtggaacca ggaacgatt 
      cgatcagct gccgaaatg ttttacgat tttattttg 
      aatggtaca agtgatgat tttgcggca actgaagga 
      tcaagttgg gccccgact ttggaaatc aaaaatgag 
      gattctttt attagtgaa aaaatgttc aaattgttg 
      aaaagtggt aaatttgtt aaagtgcct ttaatgatt 
      gggatgacc actgaggaa aatttatca atggaccac 
      aaaaatttt gacaaaaat gtcgaacag tatgatatt 
      tcttataaa actttggtg ccggttgac ataacagga 
      gatgaagaa aaattaaat gaaattggt cgaaagatt 
      cgagacatg tacacagga gggaaaaac ttttcgaaa 
      aatattgga gacgccatt cgattttcc agtgataat 
      gacatgact cgcccgatt atgaaacaa gccgagatt 
      tactcaaaa ttcgctcca acttacttt taccaattt 
      tcttacgac ggaaatttt gcagggttt agtctaact 
      tatccaggt actggaagt gttgctcat gccgaggaa 
      ttatattat ctttttgat tttattcat gacatcaaa 
      agttttcca aaagaagat caaattgta cgaaatcgt 
      ttaattaaa ctgtggacc aattttgcc aaatttagg 
      aatccgaca ccaacgcta atggaaaat gtttcttgg 
      ccacaacta aaagtggag gaaaatgat tttccatat 
      cttgatatt ggagcaaat ttgacagtt aagaatttc 
      cccaaaaaa gaaatttat aaaaaatgg agtcaaata 
      tatgaaagt ttaggacat gatgatttt gatacttat 
      taa
    mRNA sequence
    • >TC016251 RA
      aatcgttta tgaaattaa agtcatttg atacaaacg 
      cctcccaac aaacatgaa gtgtattca actatttgt 
      gtttttctc actaaaaat tggggtcga tctgagtga 
      ccctttggt ggaactccc aaacggctt aataagagg 
      gcaaagaat aaaaaccca aaacaatga attctactc 
      tttcaaaaa aataccata tgcccaacc acctattgg 
      tactttgcg atttcaagc ccctcgtga acccaggaa 
      ttgggacgg tgttttgga ctgcaccac tatggacgt 
      tgtttgtta ccagatgtt taacaaatt ggagaatca 
      atcagaaaa ttgcctttt tttaaatgt ttacacacc 
      caacttgcc ggagtttgt aaatgagtc atttccagt 
      catggtata catccatgg tggtggttt cacagaagg 
      aatgaccca acaatacga cccacggct ctttgtgga 
      aaatggggt ggttttagt tacttttaa ctaccgttt 
      aggaccatt tggttttct ctccactca agatgatac 
      gatccccgg aaacaacgg gttaaaaga tcagaatat 
      ggccttgaa atgggtgca caaaaatat tgaattttt 
      cagtgggga ccctcaaaa agtcacact ttttgggca 
      cagtgcagg gtcagcttc agtcggtta tcacctcct 
      taacctcaa atcggaaga tttattttg ggccgcaat 
      ttgtcaaag tgggtccgt tttagggcc atgggccta 
      tcaaaggca accgagacg acaagcttt cctcactgc 
      tagttttat aaatgagac atttcttga gcaaaatga 
      ttcgaaaaa attgcttga atttttgca aaacgtgga 
      accaggaac gattcgatc agctgccga aatgtttta 
      cgattttat tttgaatgg tacaagtga tgattttgc 
      ggcaactga aggatcaag ttgggcccc gactttgga 
      aatcaaaaa tgaggattc ttttattag tgaaaaaat 
      gttcaaatt gttgaaaag tggtaaatt tgttaaagt 
      gcctttaat gattgggat gaccactga ggaaaattt 
      atcaatgga ccacaaaaa ttttgacaa aaatgtcga 
      acagtatga tatttctta taaaacttt ggtgccggt 
      tgacataac aggagatga agaaaaatt aaatgaaat 
      tggtcgaaa gattcgaga catgtacac aggagggaa 
      aaacttttc gaaaaatat tggagacgc cattcgatt 
      ttccagtga taatgacat gactcgccc gattatgaa 
      acaagccga gatttactc aaaattcgc tccaactta 
      cttttacca attttctta cgacggaaa ttttgcagg 
      gtttagtct aacttatcc aggtactgg aagtgttgc 
      tcatgccga ggaattata ttatctttt tgattttat 
      tcatgacat caaaagttt tccaaaaga agatcaaat 
      tgtacgaaa tcgtttaat taaactgtg gaccaattt 
      tgccaaatt tagttttct tttaggaat ccgacacca 
      acgctaatg gaaaatgtt tcttggcca caactaaaa 
      gtggaggaa aatgatttt ccatatctt gatattgga 
      gcaaatttg acagttaag aatttcccc aaaaaagaa 
      atttataaa aaatggagt caaatatat gaaagttta 
      ggacatgat gattttgat acttattaa ttattatta 
      ttagaaaac aagtgttaa taataatca taaagacaa
    • >TC016251 RB
      aatcgttta tgaaattaa agtcatttg atacaaacg 
      cctcccaac aaacatgaa gtgtattca actatttgt 
      gtttttctc actaaaaat tggggtcga tctgagtga 
      ccctttggt ggaactccc aaacggctt aataagagg 
      gcaaagaat aaaaaccca aaacaatga attctactc 
      tttcaaaaa aataccata tgcccaacc acctattgg 
      tactttgcg atttcaagc ccctcgtga acccaggaa 
      ttgggacgg tgttttgga ctgcaccac tatggacgt 
      tgtttgtta ccagatgtt taacaaatt ggagaatca 
      atcagaaaa ttgcctttt tttaaatgt ttacacacc 
      caacttgcc ggagtttgt aaatgagtc atttccagt 
      catggtata catccatgg tggtggttt cacagaagg 
      aatgaccca acaatacga cccacggct ctttgtgga 
      aaatggggt ggttttagt tacttttaa ctaccgttt 
      aggaccatt tggttttct ctccactca agatgatac 
      gatccccgg aaacaacgg gttaaaaga tcagaatat 
      ggccttgaa atgggtgca caaaaatat tgaattttt 
      cagtgggga ccctcaaaa agtcacact ttttgggca 
      cagtgcagg gtcagcttc agtcggtta tcacctcct 
      taacctcaa atcggaaga tttattttg ggccgcaat 
      ttgtcaaag tgggtccgt tttagggcc atgggccta 
      tcaaaggca accgagacg acaagcttt cctcactgc 
      tagttttat aaatgagac atttcttga gcaaaatga 
      ttcgaaaaa attgcttga atttttgca aaacgtgga 
      accaggaac gattcgatc agctgccga aatgtttta 
      cgattttat tttgaatgg tacaagtga tgattttgc 
      ggcaactga aggatcaag ttgggcccc gactttgga 
      aatcaaaaa tgaggattc ttttattag tgaaaaaat 
      gttcaaatt gttgaaaag tggtaaatt tgttaaagt 
      gcctttaat gattgggat gaccactga ggaaaattt 
      atcaatgga ccacaaaaa ttttgacaa aaatgtcga 
      acagtatga tatttctta taaaacttt ggtgccggt 
      tgacataac aggagatga agaaaaatt aaatgaaat 
      tggtcgaaa gattcgaga catgtacac aggagggaa 
      aaacttttc gaaaaatat tggagacgc cattcgatt 
      ttccagtga taatgacat gactcgccc gattatgaa 
      acaagccga gatttactc aaaattcgc tccaactta 
      cttttacca attttctta cgacggaaa ttttgcagg 
      gtttagtct aacttatcc aggtactgg aagtgttgc 
      tcatgccga ggaattata ttatctttt tgattttat 
      tcatgacat caaaagttt tccaaaaga agatcaaat 
      tgtacgaaa tcgtttaat taaactgtg gaccaattt 
      tgccaaatt taggaatcc gacaccaac gctaatgga 
      aaatgtttc ttggccaca actaaaagt ggaggaaaa 
      tgattttcc atatcttga tattggagc aaatttgac 
      agttaagaa tttccccaa aaaagaaat ttataaaaa 
      atggagtca aatatatga aagtttagg acatgatga 
      ttttgatac ttattaatt attattatt agaaaacaa 
      gtgttaata ataatcata aagacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY