Status
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Gene information
General information
TC031426
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016212,TC016211
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4348516..4365157
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031426
    iBeetle screen iB_05977

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031426 RA
      MMFKSLFFV ITLVVTQSL AADHNTFKD CSRVDFCTN 
      LRTRQPSND YSVDPDTIT TNANTLSAT LRPNSGGTD 
      LVLTLTGLQ EDTFRVKIK EVDSGRYEL VDVLDGEPK 
      PTDFNGVAR DDTSITVST NSNSNSARV TFSPFSIEF 
      SKDGLLEVV FDGDKLTLL ETEVNAPFS FGVKFPQAV 
      QLYGIHEHC ETLALKNTA PGGTDPYRL KNSDVGGFE 
      IESPMALYG SIPVLYGHG PKATSGVFL HNAAQQWVE 
      TTSSSAGTT QAYFMTEAG CLDLFVLLG PTPTQVVRQ 
      YTQLTGTAH LPQIWVLGY HQSRYSYDT QELVKDVAA 
      NFDSHNFQL DAIWLDIDY TDGFKYFTW NPDTFSDPV 
      EMQNVLNGT NKKLVTIID PHIKVEEGY NVYDGALAK 
      DLFVKNADG SVFQGSCWP GLSSYMDFL NPEARDFYG 
      SMYSYENFQ STTATLAGI WNDMNEPSV FDNSLEMTL 
      PADAIHHGN VKHQEIHNI YGFLHTMST HKGLLERDN 
      ATKRPFILT RSHFAGSQR FAAIWTGDN TADWPYLLA 
      EVQECLNSN ILGIVLCGS DVGGFFNNP SNELYERWY 
      QLGAWLPFF RAHSTKDAE RREPYLKPE DTQGVVRTA 
      LQTRYKHLP VWYTLAFEH TITGDPIVR PLFYQYPED 
      VSVYKIDDQ LLLGRDILV RAVAEAGVE SVDVYFPGG 
      GNEVWVSET SDDVQAGNG KVAIPVTRE NIPVYYRRG 
      GIVVRKDTV RSNTAEMAS DSFTIYYTV DENNTAHGT 
      LYIDDGVSF KYRDENDYT YVNIEVSGD NVTISNIQG 
      DGNYPVIIE KIVRRDIVQ MPEGSQSGR YKEIVHTTD 
      ASGQLLKNV KIDPKNKTV LRLK
    CDS sequence
    • >TC031426 RA
      atgatgttc aagtcttta ttttttgta atcactttg 
      gtggtaaca cagagcctt gcagccgac cataacact 
      ttcaaagat tgtagccga gttgatttt tgcactaat 
      ttacgaaca agacaacca tcaaatgac tattcggta 
      gatcctgac accatcacc actaatgca aacacgctc 
      agtgctact cttagacct aacagtggt ggtaccgac 
      ctggtactc acccttaca ggcctccag gaagacact 
      ttccgggta aaaataaag gaagttgat tcaggacgg 
      tacgagctg gttgatgtt ctcgatgga gaaccaaaa 
      ccaaccgat ttcaacggt gtggcacgc gatgatacg 
      tccataaca gtctcaaca aactcaaat tcgaactca 
      gcccgtgtc acattctcg ccatttagt atcgagttc 
      agtaaagac gggcttttg gaggtggtt ttcgatggc 
      gataaacta actttactt gagacggaa gttaacgcc 
      ccgttcagt ttcggcgta aaattccca caagccgtc 
      caactttac ggcattcac gaacactgc gaaacccta 
      gctttgaaa aacacagca ccaggtggt accgacccg 
      taccgtctc aaaaactca gacgttggt gggtttgaa 
      attgagtca cccatggct ctttatggc tcaatcccg 
      gtgttatac ggacatggc cctaaagcc acttctgga 
      gtgttcctc cataacgcg gcccaacaa tgggtggaa 
      accacgagt tcaagtgcc ggtaccacc caggcctac 
      tttatgaca gaagctggg tgcctcgac ttgttcgtt 
      ttgctgggt cctacaccc acacaagtc gtacgacag 
      tacacccag ctgactggt accgcccat ctgccccag 
      atctgggtg ctagggtac caccagtcg cggtacagt 
      tacgacacc caggaactg gtcaaagac gtggctgcg 
      aactttgac agtcacaat ttccagttg gatgcaatt 
      tggctggac attgactac accgacggg ttcaagtac 
      ttcacttgg aaccctgac acgttcagc gatccggtg 
      gaaatgcaa aacgtgctc aatggtact aacaagaag 
      ctagtgacc attattgac ccccatatt aaagttgag 
      gagggctat aatgtgtac gatggggct ttggccaag 
      gatttgttc gtgaaaaat gcagatggg tcggttttt 
      caaggctcg tgttggccg ggacttagc agttatatg 
      gattttttg aacccggaa gctagggat ttttatggg 
      agtatgtac tcgtatgag aattttcaa agtacgact 
      gctactctc gccgggatt tggaacgac atgaatgaa 
      ccctcagtg tttgacaat agcttggaa atgactctg 
      ccggcagac gccatccac catggcaat gtcaagcac 
      caggaaatc cacaacatt tatggattc ctccataca 
      atgtcaacc cacaaaggc ctcctggag cgtgacaat 
      gccaccaaa cgtcccttc atcctaact cgctcccac 
      tttgccggt tcccaacga tttgccgcc atctggacc 
      ggtgataac accgcagat tggccttat ctcttagcc 
      gaagtccag gaatgtcta aactcaaat attctggga 
      atcgtcctc tgtggctca gacgttggc ggttttttc 
      aacaaccct tcaaacgaa ctttacgaa aggtggtac 
      caacttggt gcttggctc cctttcttc cgagctcat 
      tcgactaaa gacgccgaa cggcgggag ccctatctc 
      aagcctgag gacactcaa ggtgtggtc aggacggcc 
      ctccagacc agatacaag catttgcca gtgtggtac 
      actttggcg tttgaacat acgataaca ggggaccca 
      attgttcgg ccacttttc taccaatat ccagaagat 
      gtcagtgtt tataaaatc gatgaccaa cttcttctg 
      ggacgcgat atcctggtg agggctgtg gcagaagcg 
      ggggttgaa tctgttgat gtttatttt cctggtggt 
      ggaaacgaa gtttgggtt tccgaaacc agtgatgat 
      gtacaggct ggcaatggg aaagttgcg attccagtg 
      accagagaa aatattcct gtttattac agaagggga 
      ggaatcgtt gtgaggaaa gatacagtt aggtcaaat 
      actgcagaa atggcgagt gattctttt acaatctat 
      tatacagtc gatgagaat aacacagca catgggact 
      ctctatatt gacgatggt gtcagtttc aaatatcgg 
      gatgaaaat gattacacg tatgttaat attgaagtt 
      tcaggagac aatgttaca atttcaaac attcaaggt 
      gacggcaat tatcccgta attattgaa aaaattgtg 
      cgtcgagat atcgttcaa atgcctgaa ggaagtcag 
      tctggacga tacaaagaa attgtccac actacagac 
      gcttctgga cagttatta aaaaatgtt aagattgat 
      ccgaaaaat aaaactgta ttgagactt aaataa
    mRNA sequence
    • >TC031426 RA
      tgtcaccta taagtagag aattttcca gttcagttt 
      aaacattgc caacaatga tgttcaagt ctttatttt 
      ttgtaatca ctttggtgg taacacaga gccttgcag 
      ccgaccata acactttca aagattgta gccgagttg 
      atttttgca ctaatttac gaacaagac aaccatcaa 
      atgactatt cggtagatc ctgacacca tcaccacta 
      atgcaaaca cgctcagtg ctactctta gacctaaca 
      gtggtggta ccgacctgg tactcaccc ttacaggcc 
      tccaggaag acactttcc gggtaaaaa taaaggaag 
      ttgattcag gacggtacg agctggttg atgttctcg 
      atggagaac caaaaccaa ccgatttca acggtgtgg 
      cacgcgatg atacgtcca taacagtct caacaaact 
      caaattcga actcagccc gtgtcacat tctcgccat 
      ttagtatcg agttcagta aagacgggc ttttggagg 
      tggttttcg atggcgata aactaactt tacttgaga 
      cggaagtta acgccccgt tcagtttcg gcgtaaaat 
      tcccacaag ccgtccaac tttacggca ttcacgaac 
      actgcgaaa ccctagctt tgaaaaaca cagcaccag 
      gtggtaccg acccgtacc gtctcaaaa actcagacg 
      ttggtgggt ttgaaattg agtcaccca tggctcttt 
      atggctcaa tcccggtgt tatacggac atggcccta 
      aagccactt ctggagtgt tcctccata acgcggccc 
      aacaatggg tggaaacca cgagttcaa gtgccggta 
      ccacccagg cctacttta tgacagaag ctgggtgcc 
      tcgacttgt tcgttttgc tgggtccta cacccacac 
      aagtcgtac gacagtaca cccagctga ctggtaccg 
      cccatctgc cccagatct gggtgctag ggtaccacc 
      agtcgcggt acagttacg acacccagg aactggtca 
      aagacgtgg ctgcgaact ttgacagtc acaatttcc 
      agttggatg caatttggc tggacattg actacaccg 
      acgggttca agtacttca cttggaacc ctgacacgt 
      tcagcgatc cggtggaaa tgcaaaacg tgctcaatg 
      gtactaaca agaagctag tgaccatta ttgaccccc 
      atattaaag ttgaggagg gctataatg tgtacgatg 
      gggctttgg ccaaggatt tgttcgtga aaaatgcag 
      atgggtcgg tttttcaag gctcgtgtt ggccgggac 
      ttagcagtt atatggatt ttttgaacc cggaagcta 
      gggattttt atgggagta tgtactcgt atgagaatt 
      ttcaaagta cgactgcta ctctcgccg ggatttgga 
      acgacatga atgaaccct cagtgtttg acaatagct 
      tggaaatga ctctgccgg cagacgcca tccaccatg 
      gcaatgtca agcaccagg aaatccaca acatttatg 
      gattcctcc atacaatgt caacccaca aaggcctcc 
      tggagcgtg acaatgcca ccaaacgtc ccttcatcc 
      taactcgct cccactttg ccggttccc aacgatttg 
      ccgccatct ggaccggtg ataacaccg cagattggc 
      cttatctct tagccgaag tccaggaat gtctaaact 
      caaatattc tgggaatcg tcctctgtg gctcagacg 
      ttggcggtt ttttcaaca acccttcaa acgaacttt 
      acgaaaggt ggtaccaac ttggtgctt ggctccctt 
      tcttccgag ctcattcga ctaaagacg ccgaacggc 
      gggagccct atctcaagc ctgaggaca ctcaaggtg 
      tggtcagga cggccctcc agaccagat acaagcatt 
      tgccagtgt ggtacactt tggcgtttg aacatacga 
      taacagggg acccaattg ttcggccac ttttctacc 
      aatatccag aagatgtca gtgtttata aaatcgatg 
      accaacttc ttctgggac gcgatatcc tggtgaggg 
      ctgtggcag aagcggggg ttgaatctg ttgatgttt 
      attttcctg gtggtggaa acgaagttt gggtttccg 
      aaaccagtg atgatgtac aggctggca atgggaaag 
      ttgcgattc cagtgacca gagaaaata ttcctgttt 
      attacagaa ggggaggaa tcgttgtga ggaaagata 
      cagttaggt caaatactg cagaaatgg cgagtgatt 
      cttttacaa tctattata cagtcgatg agaataaca 
      cagcacatg ggactctct atattgacg atggtgtca 
      gtttcaaat atcgggatg aaaatgatt acacgtatg 
      ttaatattg aagtttcag gagacaatg ttacaattt 
      caaacattc aaggtgacg gcaattatc ccgtaatta 
      ttgaaaaaa ttgtgcgtc gagatatcg ttcaaatgc 
      ctgaaggaa gtcagtctg gacgataca aagaaattg 
      tccacacta cagacgctt ctggacagt tattaaaaa 
      atgttaaga ttgatccga aaaataaaa ctgtattga 
      gacttaaat aaaatgatt tttccatga tctgtgttt 
      attatcttc atgattctg atctttaat ttaataaat 
      cccttgaga aagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY