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Gene information
General information
TC016205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4399460..4403907
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016205
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05974

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016205 RA
      MVIHCSGYL KVKQFTASS SPYEGCYQN MGLVAVGHS 
      LPPSAITEI KLHANMFMF RASLDLKLI FLDARVAQL 
      TGYEPQDLI EKTLYHYIH GSDIMPMRF AHHQLLCKG 
      QVTTKYYRF LSKGGGWVW MQSYATIVH NSRSSRPHC 
      IVSVNYVLS DLEGKDLLL NSAQGSLRE EVPTSPKSS 
      PKPLPKYPP SRATPQQPP IISPSIPDD DFSDSAITY 
      PTPEYSMFL SLPPSEEPY YSPHQELFY PYSDPEITQ 
      HHVINQSQR PFSASSSSC SSSESELHL QNLSSNPYC 
      NDSGLHGQH FNLSCFNNN QQQQQYQLN HVGSPAGYT 
      SVIVDTQQY QLANEYVH
    CDS sequence
    • >TC016205 RA
      atggtgatc cactgttcg ggctacctc aaagtgaag 
      caattcacc gcctcctcg tcgccctac gaaggctgc 
      taccagaac atgggcctc gtcgccgtc ggccactct 
      ctacctccc agtgccatc accgaaatc aagctgcac 
      gccaacatg ttcatgttt cgggccagt cttgacttg 
      aaattgatt tttctggac gctagggtg gcccaactg 
      acgggatac gagccgcag gatttgatc gaaaaaaca 
      ctctatcac tacatacat gggagcgat atcatgccc 
      atgaggttc gcacaccac cagttgttg tgcaagggc 
      caagtgaca acaaaatac taccgattt ttgagcaaa 
      ggcggcggc tgggtgtgg atgcaaagc tacgccacc 
      atcgtccac aactcgcgt tcttccaga ccacactgt 
      atagtctca gtcaactat gttttaagc gaccttgaa 
      ggcaaagac ctgctcctg aactcagcg caaggctcc 
      ctccgggag gaggtcccc acctccccc aagagctcc 
      cccaagccg ctccccaag tacccccca agccgcgcg 
      accccgcaa caaccccca atcatctcc ccctcgatc 
      cctgacgac gatttcagc gactctgca attacctac 
      cccaccccc gagtactcc atgttcctc tccctccct 
      cccagcgaa gaaccgtac tactccccc caccaggag 
      ctgttctac ccctactcc gaccccgaa atcacccaa 
      caccacgtc atcaaccaa tcacagcgg ccgttcagt 
      gcttcgtct tcgagctgt agctcgtct gagagtgag 
      cttcatctt cagaatctc agctcgaat ccgtattgc 
      aatgacagt gggttacat ggacaacat ttcaatctt 
      tcgtgtttt aataataat cagcaacag cagcagtat 
      cagctgaat catgttggc agccctgcg ggctacacc 
      agtgtcatt gtggacacg cagcagtac caacttgcc 
      aacgaatac gttcactag
    mRNA sequence
    • >TC016205 RA
      gtacttgac caccggcaa aatggtgat ccactgttc 
      gggctacct caaagtgaa gcaattcac cgcctcctc 
      gtcgcccta cgaaggctg ctaccagaa catgggcct 
      cgtcgccgt cggccactc tctacctcc cagtgccat 
      caccgaaat caagctgca cgccaacat gttcatgtt 
      tcgggccag tcttgactt gaaattgat ttttctgga 
      cgctagggt ggcccaact gacgggata cgagccgca 
      ggatttgat cgaaaaaac actctatca ctacataca 
      tgggagcga tatcatgcc catgaggtt cgcacacca 
      ccagttgtt gtgcaaggg ccaagtgac aacaaaata 
      ctaccgatt tttgagcaa aggcggcgg ctgggtgtg 
      gatgcaaag ctacgccac catcgtcca caactcgcg 
      ttcttccag accacactg tatagtctc agtcaacta 
      tgttttaag cgaccttga aggcaaaga cctgctcct 
      gaactcagc gcaaggctc cctccggga ggaggtccc 
      cacctcccc caagagctc ccccaagcc gctccccaa 
      gtacccccc aagccgcgc gaccccgca acaaccccc 
      aatcatctc cccctcgat ccctgacga cgatttcag 
      cgactctgc aattaccta ccccacccc cgagtactc 
      catgttcct ctccctccc tcccagcga agaaccgta 
      ctactcccc ccaccagga gctgttcta cccctactc 
      cgaccccga aatcaccca acaccacgt catcaacca 
      atcacagcg gccgttcag tgcttcgtc ttcgagctg 
      tagctcgtc tgagagtga gcttcatct tcagaatct 
      cagctcgaa tccgtattg caatgacag tgggttaca 
      tggacaaca tttcaatct ttcgtgttt taataataa 
      tcagcaaca gcagcagta tcagctgaa tcatgttgg 
      cagccctgc gggctacac cagtgtcat tgtggacac 
      gcagcagta ccaacttgc caacgaata cgttcacta 
      gtgagctat ctatatcat catgtactg tacatttta 
      ttttttgtg taagttttt gatttattt atggaacgt 
      tttgtaaat agaagtgag tccccgtgg tgtgccata 
      cgatgaaga gtttgtttc gtgtaatta tcattttga 
      gtacttatt attgtttgg atagatacg acaataatt 
      taagactgt gaataccat atgctgtaa atagtctat 
      gtacaataa ctactttac tattacgta ttcccaacc 
      aaatatatt aataaaaat tgctgttat tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY