| General information | |||
| TC031973
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 34677..67981 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031973 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05966 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031973 RA
MTATAEQVH DFTDELSEM VKITIVLTV FLLLLMCLL LVGITLSIA KICSTSPGS ASCQLIGRS QEVACNGNQ HGVDCLLNI QKNETHLGK ITGDEALLA AGVDLSEDV VVLGKITSH HPPSETVVI VNNDIAWNF VSLEGKTLC HPGYNHDST FSYFMLQEL ENKIIESKS PNCDNITLL ERHIKQLSE FFGPSCRPG PWTQNDKFD KTLKTKYSS LCKLCTNEH CNENYPQPF NDSLKCLLR KNADVAVTT LQEAQLFFQ NVANSNRYK YMCKDGSVK SSSTPCSWT KTLPDLVVA HRSVVGQMK NFLQKNLGS HINYNLTLT RDTSNNKYE EPLVDLLEL QQRKITFLE NNGDFLSLK KFVNDYTTV PNPETSKQC DRTLRWCTY SDLETKKCK WLRQAAVNW GIQPVVECV QSEGDDELS CLDDLKKGS ADIVVASSN YAYISLKKE LTSIAFPET DTRYLSQIF VVKRADDSS IKSFSDLKG KSACIPQFG GKEHLSLID TTRRLGITD DTQCDYGKL LNDFIGDSC VPGVRSSLI KDSTDLVDK DKMCRRCKA INQTKTAEY CNADVINAY FASQGAVNC LLNNDGDFA VVSKKDVLN DTSLTALCK NDSLASMNG LLNVNTDCA LFVLASSEV VLKNDSSKK SDIQLALYE FEKWFGQKL RKPFHLFDK FDNEPDLLF LDSTPGLNF EGNLPYMEE YQRLLNNVL DCNTSGSAS VISSMSFII SFVLLTLCF TF
- >TC031973 RB
MWCCNIFSI FVSISLFGT SCSSQDIID YSKICSTSP GSASCQLIG RSQEVACNG NQHGVDCLL NIQKNETHL GKITGDEAL LAAGVDLSE DVVVLGKIT SHHPPSETV VIVNNDIAW NFVSLEGKT LCHPGYNHD STFSYFMLQ ELENKIIES KSPNCDNIT LLERHIKQL SEFFGPSCR PGPWTQNDK FDKTLKTKY SSLCKLCTN EHCNENYPQ PFNDSLKCL LRKNADVAV TTLQEAQLF FQNVANSNR YKYMCKDGS VKSSSTPCS WTKTLPDLV VAHRSVVGQ MKNFLQKNL GSHINYNLT LTRDTSNNK YEEPLVDLL ELQQRKITF LENNGDFLS LKKFVNDYT TVPNPETSK QCDRTLRWC TYSDLETKK CKWLRQAAV NWGIQPVVE CVQSEGDDE LSCLDDLKK GSADIVVAS SNYAYISLK KELTSIAFP ETDTRYLSQ IFVVKRADD SSIKSFSDL KGKSACIPQ FGGKEHLSL IDTTRRLGI TDDTQCDYG KLLNDFIGD SCVPGVRSS LIKDSTDLV DKDKMCRRC KAINQTKTA EYCNADVIN AYFASQGAV NCLLNNDGD FAVVSKKDV LNDTSLTAL CKNDSLASM NGLLNVNTD CALFVLASS EVVLKNDSS KKSDIQLAL YEFEKWFGQ KLRKPFHLF DKFDNEPDL LFLDSTPGL NFEGNLPYM EEYQRLLNN VLDCNTSGS ASVISSMSF IISFVLLTL CFTF
- >TC031973 RC
MWCCNIFSI FVSISLFGT SCSSQDIID YSKICSTSP GSASCQLIG RSQEVACNG NQHGVDCLL NIQKNETHL GKITGDEAL LAAGVDLSE DVVVLGKIT SQTKYSSLC KLCTNEHCN ENYPQPFND SLKCLLRKN ADVAVTTLQ EAQLFFQNV ANSNRYKYM CKDGSVKSS STPCSWTKT LPDLVVAHR SVVGQMKNF LQKNLGSHI NYNLTLTRD TSNNKYEEP LVDLLELQQ RKITFLENN GDFLSLKKF VNDYTTVPN PETSKQCDR TLRWCTYSD LETKKCKWL RQAAVNWGI QPVVECVQS EGDDELSCL DDLKKGSAD IVVASSNYA YISLKKELT SIAFPETDT RYLSQIFVV KRADDSSIK SFSDLKGKS ACIPQFGGK EHLSLIDTT RRLGITDDT QCDYGKLLN DFIGDSCVP GVRSSLIKD STDLVDKDK MCRRCKAIN QTKTAEYCN ADVINAYFA SQGAVNCLL NNDGDFAVV SKKDVLNDT SLTALCKND SLASMNGLL NVNTDCALF VLASSEVVL KNDSSKKSD IQLALYEFE KWFGQKLRK PFHLFDKFD NEPDLLFLD STPGLNFEG NLPYMEEYQ RLLNNVLDC NTSGSASVI SSMSFIISF VLLTLCFTF
- >TC031973 RA
atgacggca acagcggag caagtccac gattttact gatgagctg tcggaaatg gtgaagata accattgtg ttaactgtc ttcttgttg ctgttgatg tgcttgcta ttggtggga attacactc agcatagca aaaatatgt tcaacatcg cctggtagt gctagttgc caactaata ggcagaagt caagaagta gcatgtaat ggcaatcaa catggcgtc gactgtctt ttaaatatt caaaagaac gaaactcac cttggtaaa atcacaggt gatgaagct ttgttagct gcaggagtt gatttatcc gaagatgta gttgttttg ggaaaaatt actagtcat catcccccc tccgaaact gtagttatt gttaacaac gacattgct tggaatttt gtgagtttg gaaggcaaa actctatgt catccaggt tataaccac gattcgact ttctcctac tttatgtta caagagctg gaaaacaaa ataattgaa tcaaaatct cccaactgt gacaatatc actttgctg gaaagacac ataaaacaa ctcagtgaa ttttttggt ccttcgtgc agaccagga ccttggact cagaacgac aaatttgat aaaacgcta aagacgaaa tattcttcg ttatgcaag ctttgtacc aacgaacat tgcaacgag aattatcca caaccattc aacgactca ctcaagtgc ttactcaga aaaaatgca gatgtggcc gttacaacg ttgcaagag gcacaatta tttttccaa aatgttgct aatagcaat agatataaa tatatgtgc aaagatggc agtgttaag tcgtcctca actccttgt tcttggacc aaaacactt ccagacctt gtagttgca cacaggagt gtggttgga caaatgaaa aactttcta cagaagaat ttaggctct cacataaat tacaactta acgctaacg agagacacg tcgaataat aaatacgaa gaaccttta gtcgatttg ttggagcta cagcagcga aaaataaca ttcttagaa aataatggc gatttttta agtttgaaa aaatttgtt aatgactat accacagtt ccaaaccca gaaacgtcc aaacaatgt gatcgcact ttaaggtgg tgcacttat tccgacttg gaaacaaaa aaatgcaaa tggttgagg caagctgct gtcaactgg ggaattcaa ccagttgtt gaatgtgtt cagagtgaa ggcgatgac gaattgtcg tgtttggat gaccttaaa aaaggaagt gctgatatc gttgtggct tcctctaac tatgcttac atatcgttg aagaaagaa ctgacaagt atagcgttc ccagaaaca gacacgaga tatttatcg caaattttt gtcgttaaa agagctgat gacagttca atcaaatcg tttagtgac ttgaagggc aaatcggct tgcatacct cagtttgga ggaaaagaa cacttgtca ttgattgat acaacacga agacttgga attactgac gatacacag tgtgattac ggaaaacta ttgaatgat tttattggt gactcgtgt gtccctggc gtacgatcg tcactcata aaggactca acagatctc gttgataag gataaaatg tgtaggcgt tgcaaagcc ataaaccaa accaaaact gctgaatat tgcaatgct gacgtgatt aatgcatat tttgccagc caaggtgcc gtcaattgt ctcttgaat aatgatgga gattttgca gttgtttcg aaaaaagat gtattgaat gatacaagt ctcacagct ttgtgtaaa aatgactct ttggcatca atgaatggt ttattaaac gtcaacact gactgcgca ttgttcgta cttgcgagc agtgaagtt gttttaaaa aacgatagc agtaaaaaa agtgacata caattggcc ctctacgaa tttgaaaaa tggtttggt caaaaatta aggaagccg ttccatttg ttcgacaaa tttgataat gaaccagac ctattgttt ctggattcc actcctgga ttaaacttt gagggaaat ttgccatat atggaagaa taccaaagg ttactaaat aacgtgctt gattgtaat acaagtggc tcagcaagc gtgatatca tcaatgtct tttattatt agtttcgtt ttattgact ttgtgtttc actttttag
- >TC031973 RB
atgtggtgc tgcaatatt ttcagtata tttgtctcg atatccctt tttggaacc tcgtgttcc agtcaagac attattgat tactcaaaa atatgttca acatcgcct ggtagtgct agttgccaa ctaataggc agaagtcaa gaagtagca tgtaatggc aatcaacat ggcgtcgac tgtctttta aatattcaa aagaacgaa actcacctt ggtaaaatc acaggtgat gaagctttg ttagctgca ggagttgat ttatccgaa gatgtagtt gttttggga aaaattact agtcatcat cccccctcc gaaactgta gttattgtt aacaacgac attgcttgg aattttgtg agtttggaa ggcaaaact ctatgtcat ccaggttat aaccacgat tcgactttc tcctacttt atgttacaa gagctggaa aacaaaata attgaatca aaatctccc aactgtgac aatatcact ttgctggaa agacacata aaacaactc agtgaattt tttggtcct tcgtgcaga ccaggacct tggactcag aacgacaaa tttgataaa acgctaaag acgaaatat tcttcgtta tgcaagctt tgtaccaac gaacattgc aacgagaat tatccacaa ccattcaac gactcactc aagtgctta ctcagaaaa aatgcagat gtggccgtt acaacgttg caagaggca caattattt ttccaaaat gttgctaat agcaataga tataaatat atgtgcaaa gatggcagt gttaagtcg tcctcaact ccttgttct tggaccaaa acacttcca gaccttgta gttgcacac aggagtgtg gttggacaa atgaaaaac tttctacag aagaattta ggctctcac ataaattac aacttaacg ctaacgaga gacacgtcg aataataaa tacgaagaa cctttagtc gatttgttg gagctacag cagcgaaaa ataacattc ttagaaaat aatggcgat tttttaagt ttgaaaaaa tttgttaat gactatacc acagttcca aacccagaa acgtccaaa caatgtgat cgcacttta aggtggtgc acttattcc gacttggaa acaaaaaaa tgcaaatgg ttgaggcaa gctgctgtc aactgggga attcaacca gttgttgaa tgtgttcag agtgaaggc gatgacgaa ttgtcgtgt ttggatgac cttaaaaaa ggaagtgct gatatcgtt gtggcttcc tctaactat gcttacata tcgttgaag aaagaactg acaagtata gcgttccca gaaacagac acgagatat ttatcgcaa atttttgtc gttaaaaga gctgatgac agttcaatc aaatcgttt agtgacttg aagggcaaa tcggcttgc atacctcag tttggagga aaagaacac ttgtcattg attgataca acacgaaga cttggaatt actgacgat acacagtgt gattacgga aaactattg aatgatttt attggtgac tcgtgtgtc cctggcgta cgatcgtca ctcataaag gactcaaca gatctcgtt gataaggat aaaatgtgt aggcgttgc aaagccata aaccaaacc aaaactgct gaatattgc aatgctgac gtgattaat gcatatttt gccagccaa ggtgccgtc aattgtctc ttgaataat gatggagat tttgcagtt gtttcgaaa aaagatgta ttgaatgat acaagtctc acagctttg tgtaaaaat gactctttg gcatcaatg aatggttta ttaaacgtc aacactgac tgcgcattg ttcgtactt gcgagcagt gaagttgtt ttaaaaaac gatagcagt aaaaaaagt gacatacaa ttggccctc tacgaattt gaaaaatgg tttggtcaa aaattaagg aagccgttc catttgttc gacaaattt gataatgaa ccagaccta ttgtttctg gattccact cctggatta aactttgag ggaaatttg ccatatatg gaagaatac caaaggtta ctaaataac gtgcttgat tgtaataca agtggctca gcaagcgtg atatcatca atgtctttt attattagt ttcgtttta ttgactttg tgtttcact ttttag
- >TC031973 RC
atgtggtgc tgcaatatt ttcagtata tttgtctcg atatccctt tttggaacc tcgtgttcc agtcaagac attattgat tactcaaaa atatgttca acatcgcct ggtagtgct agttgccaa ctaataggc agaagtcaa gaagtagca tgtaatggc aatcaacat ggcgtcgac tgtctttta aatattcaa aagaacgaa actcacctt ggtaaaatc acaggtgat gaagctttg ttagctgca ggagttgat ttatccgaa gatgtagtt gttttggga aaaattact agtcagacg aaatattct tcgttatgc aagctttgt accaacgaa cattgcaac gagaattat ccacaacca ttcaacgac tcactcaag tgcttactc agaaaaaat gcagatgtg gccgttaca acgttgcaa gaggcacaa ttatttttc caaaatgtt gctaatagc aatagatat aaatatatg tgcaaagat ggcagtgtt aagtcgtcc tcaactcct tgttcttgg accaaaaca cttccagac cttgtagtt gcacacagg agtgtggtt ggacaaatg aaaaacttt ctacagaag aatttaggc tctcacata aattacaac ttaacgcta acgagagac acgtcgaat aataaatac gaagaacct ttagtcgat ttgttggag ctacagcag cgaaaaata acattctta gaaaataat ggcgatttt ttaagtttg aaaaaattt gttaatgac tataccaca gttccaaac ccagaaacg tccaaacaa tgtgatcgc actttaagg tggtgcact tattccgac ttggaaaca aaaaaatgc aaatggttg aggcaagct gctgtcaac tggggaatt caaccagtt gttgaatgt gttcagagt gaaggcgat gacgaattg tcgtgtttg gatgacctt aaaaaagga agtgctgat atcgttgtg gcttcctct aactatgct tacatatcg ttgaagaaa gaactgaca agtatagcg ttcccagaa acagacacg agatattta tcgcaaatt tttgtcgtt aaaagagct gatgacagt tcaatcaaa tcgtttagt gacttgaag ggcaaatcg gcttgcata cctcagttt ggaggaaaa gaacacttg tcattgatt gatacaaca cgaagactt ggaattact gacgataca cagtgtgat tacggaaaa ctattgaat gattttatt ggtgactcg tgtgtccct ggcgtacga tcgtcactc ataaaggac tcaacagat ctcgttgat aaggataaa atgtgtagg cgttgcaaa gccataaac caaaccaaa actgctgaa tattgcaat gctgacgtg attaatgca tattttgcc agccaaggt gccgtcaat tgtctcttg aataatgat ggagatttt gcagttgtt tcgaaaaaa gatgtattg aatgataca agtctcaca gctttgtgt aaaaatgac tctttggca tcaatgaat ggtttatta aacgtcaac actgactgc gcattgttc gtacttgcg agcagtgaa gttgtttta aaaaacgat agcagtaaa aaaagtgac atacaattg gccctctac gaatttgaa aaatggttt ggtcaaaaa ttaaggaag ccgttccat ttgttcgac aaatttgat aatgaacca gacctattg tttctggat tccactcct ggattaaac tttgaggga aatttgcca tatatggaa gaataccaa aggttacta aataacgtg cttgattgt aatacaagt ggctcagca agcgtgata tcatcaatg tcttttatt attagtttc gttttattg actttgtgt ttcactttt tag
- >TC031973 RA
taccctttt aattaatta ttaattaat attaattat tttattttt tgcaatttt tagaaacgt gaagaagga tggtgggtt gttattggt gatccgaaa accaattcc ttgctctcc attaaacgc cttactctt caacaaaaa gctagagtc aagttggat tttgttgcc cctagtcca ggacaccat aattacact ttatatttt atgagtgat gcgtatttg ggttgcgat caagaatat aaattttca atagatgtt ggtgatttt gaaagcagt gaaagtgag tcagagtga acgagaaat atttagttg agaataggt aaacttatt ttgttattt aagattcgt acaataaaa aatcaaaag gaagtgacg gcaataaag atattgaag agagcacag cggaagaaa cgaagtcga aggaaacat tatctaaag cagatgaag atgacggca acagcggag caagtccac gattttact gatgagctg tcggaaatg gtgaagata accattgtg ttaactgtc ttcttgttg ctgttgatg tgcttgcta ttggtggga attacactc agcatagca aaaatatgt tcaacatcg cctggtagt gctagttgc caactaata ggcagaagt caagaagta gcatgtaat ggcaatcaa catggcgtc gactgtctt ttaaatatt caaaagaac gaaactcac cttggtaaa atcacaggt gatgaagct ttgttagct gcaggagtt gatttatcc gaagatgta gttgttttg ggaaaaatt actagtcat catcccccc tccgaaact gtagttatt gttaacaac gacattgct tggaatttt gtgagtttg gaaggcaaa actctatgt catccaggt tataaccac gattcgact ttctcctac tttatgtta caagagctg gaaaacaaa ataattgaa tcaaaatct cccaactgt gacaatatc actttgctg gaaagacac ataaaacaa ctcagtgaa ttttttggt ccttcgtgc agaccagga ccttggact cagaacgac aaatttgat aaaacgcta aagacgaaa tattcttcg ttatgcaag ctttgtacc aacgaacat tgcaacgag aattatcca caaccattc aacgactca ctcaagtgc ttactcaga aaaaatgca gatgtggcc gttacaacg ttgcaagag gcacaatta tttttccaa aatgttgct aatagcaat agatataaa tatatgtgc aaagatggc agtgttaag tcgtcctca actccttgt tcttggacc aaaacactt ccagacctt gtagttgca cacaggagt gtggttgga caaatgaaa aactttcta cagaagaat ttaggctct cacataaat tacaactta acgctaacg agagacacg tcgaataat aaatacgaa gaaccttta gtcgatttg ttggagcta cagcagcga aaaataaca ttcttagaa aataatggc gatttttta agtttgaaa aaatttgtt aatgactat accacagtt ccaaaccca gaaacgtcc aaacaatgt gatcgcact ttaaggtgg tgcacttat tccgacttg gaaacaaaa aaatgcaaa tggttgagg caagctgct gtcaactgg ggaattcaa ccagttgtt gaatgtgtt cagagtgaa ggcgatgac gaattgtcg tgtttggat gaccttaaa aaaggaagt gctgatatc gttgtggct tcctctaac tatgcttac atatcgttg aagaaagaa ctgacaagt atagcgttc ccagaaaca gacacgaga tatttatcg caaattttt gtcgttaaa agagctgat gacagttca atcaaatcg tttagtgac ttgaagggc aaatcggct tgcatacct cagtttgga ggaaaagaa cacttgtca ttgattgat acaacacga agacttgga attactgac gatacacag tgtgattac ggaaaacta ttgaatgat tttattggt gactcgtgt gtccctggc gtacgatcg tcactcata aaggactca acagatctc gttgataag gataaaatg tgtaggcgt tgcaaagcc ataaaccaa accaaaact gctgaatat tgcaatgct gacgtgatt aatgcatat tttgccagc caaggtgcc gtcaattgt ctcttgaat aatgatgga gattttgca gttgtttcg aaaaaagat gtattgaat gatacaagt ctcacagct ttgtgtaaa aatgactct ttggcatca atgaatggt ttattaaac gtcaacact gactgcgca ttgttcgta cttgcgagc agtgaagtt gttttaaaa aacgatagc agtaaaaaa agtgacata caattggcc ctctacgaa tttgaaaaa tggtttggt caaaaatta aggaagccg ttccatttg ttcgacaaa tttgataat gaaccagac ctattgttt ctggattcc actcctgga ttaaacttt gagggaaat ttgccatat atggaagaa taccaaagg ttactaaat aacgtgctt gattgtaat acaagtggc tcagcaagc gtgatatca tcaatgtct tttattatt agtttcgtt ttattgact ttgtgtttc actttttag ttagaagaa aatgtataa cgactctgc ttcgaaatt ctgtgctta ataactata ttttgcatg aactgacat aacgttgtt aaagtcgtt tttaatttt catactttt taataaaat tacgatatt ttgctataa tttgaactt ttactttga atgtatgtc tagttacta aaaagagat ttttacctc atttaattt ttttgttcc aatagtccc ataccattg aacaaa
- >TC031973 RB
tgagacgta tgaaaaatg tttcaagct aataaaaag tgaatgcca acaattaaa actaaattt ccaacccaa aagtgtgac agtcgattt tgagcttgc cattcacaa cgctattaa tcgaatttg gcgtgaaat agttattat tggatgcag attccattt atcacaagc ttggtggcg aaaaattca agagttggg tctttcagt ggactataa gtcaaataa tcctactgg aaaatggct gaaatatac tttcggact tcaatattt acctccgaa cgaagtttt tgactgttt tgtttcgga acttgttga attagctcc accggagta caacgataa agttgtaaa atataatcg acattttaa tagaaactt acatttcac caatttcat caatttggg caagtgctt ctgcgagtt ctacccgca ccacaaata actgcgaat cgtttcatg cccatttta attcaagtt tttatacat ataaatgct catcaggtg tgtgcaaca atgtggtgc tgcaatatt ttcagtata tttgtctcg atatccctt tttggaacc tcgtgttcc agtcaagac attattgat tactcaaaa atatgttca acatcgcct ggtagtgct agttgccaa ctaataggc agaagtcaa gaagtagca tgtaatggc aatcaacat ggcgtcgac tgtctttta aatattcaa aagaacgaa actcacctt ggtaaaatc acaggtgat gaagctttg ttagctgca ggagttgat ttatccgaa gatgtagtt gttttggga aaaattact agtcatcat cccccctcc gaaactgta gttattgtt aacaacgac attgcttgg aattttgtg agtttggaa ggcaaaact ctatgtcat ccaggttat aaccacgat tcgactttc tcctacttt atgttacaa gagctggaa aacaaaata attgaatca aaatctccc aactgtgac aatatcact ttgctggaa agacacata aaacaactc agtgaattt tttggtcct tcgtgcaga ccaggacct tggactcag aacgacaaa tttgataaa acgctaaag acgaaatat tcttcgtta tgcaagctt tgtaccaac gaacattgc aacgagaat tatccacaa ccattcaac gactcactc aagtgctta ctcagaaaa aatgcagat gtggccgtt acaacgttg caagaggca caattattt ttccaaaat gttgctaat agcaataga tataaatat atgtgcaaa gatggcagt gttaagtcg tcctcaact ccttgttct tggaccaaa acacttcca gaccttgta gttgcacac aggagtgtg gttggacaa atgaaaaac tttctacag aagaattta ggctctcac ataaattac aacttaacg ctaacgaga gacacgtcg aataataaa tacgaagaa cctttagtc gatttgttg gagctacag cagcgaaaa ataacattc ttagaaaat aatggcgat tttttaagt ttgaaaaaa tttgttaat gactatacc acagttcca aacccagaa acgtccaaa caatgtgat cgcacttta aggtggtgc acttattcc gacttggaa acaaaaaaa tgcaaatgg ttgaggcaa gctgctgtc aactgggga attcaacca gttgttgaa tgtgttcag agtgaaggc gatgacgaa ttgtcgtgt ttggatgac cttaaaaaa ggaagtgct gatatcgtt gtggcttcc tctaactat gcttacata tcgttgaag aaagaactg acaagtata gcgttccca gaaacagac acgagatat ttatcgcaa atttttgtc gttaaaaga gctgatgac agttcaatc aaatcgttt agtgacttg aagggcaaa tcggcttgc atacctcag tttggagga aaagaacac ttgtcattg attgataca acacgaaga cttggaatt actgacgat acacagtgt gattacgga aaactattg aatgatttt attggtgac tcgtgtgtc cctggcgta cgatcgtca ctcataaag gactcaaca gatctcgtt gataaggat aaaatgtgt aggcgttgc aaagccata aaccaaacc aaaactgct gaatattgc aatgctgac gtgattaat gcatatttt gccagccaa ggtgccgtc aattgtctc ttgaataat gatggagat tttgcagtt gtttcgaaa aaagatgta ttgaatgat acaagtctc acagctttg tgtaaaaat gactctttg gcatcaatg aatggttta ttaaacgtc aacactgac tgcgcattg ttcgtactt gcgagcagt gaagttgtt ttaaaaaac gatagcagt aaaaaaagt gacatacaa ttggccctc tacgaattt gaaaaatgg tttggtcaa aaattaagg aagccgttc catttgttc gacaaattt gataatgaa ccagaccta ttgtttctg gattccact cctggatta aactttgag ggaaatttg ccatatatg gaagaatac caaaggtta ctaaataac gtgcttgat tgtaataca agtggctca gcaagcgtg atatcatca atgtctttt attattagt ttcgtttta ttgactttg tgtttcact ttttagtta gaagaaaat gtataacga ctctgcttc gaaattctg tgcttaata actatattt tgcatgaac tgacataac gttgttaaa gtcgttttt aattttcat actttttaa taaaattac gatattttg ctataattt gaactttta ctttgaatg tatgtctag ttactaaaa agagatttt tacctcatt taatttttt tgttccaat agtcccata ccattgaac aaa
- >TC031973 RC
tgagacgta tgaaaaatg tttcaagct aataaaaag tgaatgcca acaattaaa actaaattt ccaacccaa aagtgtgac agtcgattt tgagcttgc cattcacaa cgctattaa tcgaatttg gcgtgaaat agttattat tggatgcag attccattt atcacaagc ttggtggcg aaaaattca agagttggg tctttcagt ggactataa gtcaaataa tcctactgg aaaatggct gaaatatac tttcggact tcaatattt acctccgaa cgaagtttt tgactgttt tgtttcgga acttgttga attagctcc accggagta caacgataa agttgtaaa atataatcg acattttaa tagaaactt acatttcac caatttcat caatttggg caagtgctt ctgcgagtt ctacccgca ccacaaata actgcgaat cgtttcatg cccatttta attcaagtt tttatacat ataaatgct catcaggtg tgtgcaaca atgtggtgc tgcaatatt ttcagtata tttgtctcg atatccctt tttggaacc tcgtgttcc agtcaagac attattgat tactcaaaa atatgttca acatcgcct ggtagtgct agttgccaa ctaataggc agaagtcaa gaagtagca tgtaatggc aatcaacat ggcgtcgac tgtctttta aatattcaa aagaacgaa actcacctt ggtaaaatc acaggtgat gaagctttg ttagctgca ggagttgat ttatccgaa gatgtagtt gttttggga aaaattact agtcagacg aaatattct tcgttatgc aagctttgt accaacgaa cattgcaac gagaattat ccacaacca ttcaacgac tcactcaag tgcttactc agaaaaaat gcagatgtg gccgttaca acgttgcaa gaggcacaa ttatttttc caaaatgtt gctaatagc aatagatat aaatatatg tgcaaagat ggcagtgtt aagtcgtcc tcaactcct tgttcttgg accaaaaca cttccagac cttgtagtt gcacacagg agtgtggtt ggacaaatg aaaaacttt ctacagaag aatttaggc tctcacata aattacaac ttaacgcta acgagagac acgtcgaat aataaatac gaagaacct ttagtcgat ttgttggag ctacagcag cgaaaaata acattctta gaaaataat ggcgatttt ttaagtttg aaaaaattt gttaatgac tataccaca gttccaaac ccagaaacg tccaaacaa tgtgatcgc actttaagg tggtgcact tattccgac ttggaaaca aaaaaatgc aaatggttg aggcaagct gctgtcaac tggggaatt caaccagtt gttgaatgt gttcagagt gaaggcgat gacgaattg tcgtgtttg gatgacctt aaaaaagga agtgctgat atcgttgtg gcttcctct aactatgct tacatatcg ttgaagaaa gaactgaca agtatagcg ttcccagaa acagacacg agatattta tcgcaaatt tttgtcgtt aaaagagct gatgacagt tcaatcaaa tcgtttagt gacttgaag ggcaaatcg gcttgcata cctcagttt ggaggaaaa gaacacttg tcattgatt gatacaaca cgaagactt ggaattact gacgataca cagtgtgat tacggaaaa ctattgaat gattttatt ggtgactcg tgtgtccct ggcgtacga tcgtcactc ataaaggac tcaacagat ctcgttgat aaggataaa atgtgtagg cgttgcaaa gccataaac caaaccaaa actgctgaa tattgcaat gctgacgtg attaatgca tattttgcc agccaaggt gccgtcaat tgtctcttg aataatgat ggagatttt gcagttgtt tcgaaaaaa gatgtattg aatgataca agtctcaca gctttgtgt aaaaatgac tctttggca tcaatgaat ggtttatta aacgtcaac actgactgc gcattgttc gtacttgcg agcagtgaa gttgtttta aaaaacgat agcagtaaa aaaagtgac atacaattg gccctctac gaatttgaa aaatggttt ggtcaaaaa ttaaggaag ccgttccat ttgttcgac aaatttgat aatgaacca gacctattg tttctggat tccactcct ggattaaac tttgaggga aatttgcca tatatggaa gaataccaa aggttacta aataacgtg cttgattgt aatacaagt ggctcagca agcgtgata tcatcaatg tcttttatt attagtttc gttttattg actttgtgt ttcactttt tagttagaa gaaaatgta taacgactc tgcttcgaa attctgtgc ttaataact atattttgc atgaactga cataacgtt gttaaagtc gtttttaat tttcatact ttttaataa aattacgat attttgcta taatttgaa cttttactt tgaatgtat gtctagtta ctaaaaaga gatttttac ctcatttaa tttttttgt tccaatagt cccatacca ttgaacaaa
The data is licensed under a
Creative Commons Attribution 3.0 Unported License. (CC-BY)
