| General information | |||
| TC001812 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450214.1: 654..6744 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001812 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05963 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001812 RA
MEDLYEKTI ERSEQLKKA GYNLIEMWE CEWTRSKEY KKEMKQIEE EIKELEELN PRNAFFGGR TNATKLRVK GKKMKYIDI CSLYPTVQC YDDYPVGHP TKIFKPRTY NTDWYGLIK CAILPPRGL YHPVLPVKN KTKTGDEKL TFPLCQLCA KLNNQKDKC SHTESQRII RGTWCTNEV EKAIEKGYK IISIDEVWH FEKKSSYLF KGYVKAFMK IKLETSPWQ DDFESEEEY RKAVKEILG IELGKIENN PGKRAVAKI CLNSLWGKF GQRQNMGAT EYVTDVKRF YEILLDDRL DNIHINEIN ENMLQIDYK LKDCYVENN FNTNIFIAA FTTANARLR LYEMLDKLG NSVAYYDTD SIVYIDDGK NTVKTGCLL GDWTDELDD VNIRTNESK IQSSAKPYD ESKIQSSAK PYDESKIQS SAKPYDESK IQSYLEQFG YFASASTKL GNASHAESL IEINEALIR FQEYYNLPV DGTLNQETL DLIKQPRCG NKDNPTAYR VHYQKWNKT NLKWYFSLA TNEMKELAQ KAFDQWESV SNLKFEYWH SQPSAVGEY NSVKPDILI SFSNTLFQH NHNSRCQKG ICSSSFDGK GNVLAHGFF PNNDECLGI HFDKSENWY FGESSNTPD DQTNFYTVL LHEIGHTLG IEHSANNNS IMYAYYKGD IDKLTRDDM WAIQYLYGI PERSKYESI PTTTTTTTT STTTKKPIT LTPRKTDSL PDLCSLSET INTFLIANH KLYIFYKKY VWIVNLKDM SYDNKPKLI TDYLTFLPD NFQEISQIY QRPDGTILL TTNNLYYII DFPNFVVKN GYNGRSITS LGVPSGKKI NAIFSTYSG QTYIFYDNT MFIQFDECL LRPKKHGMI AELFSSIPS NVDKAFRYI NGKIYFFKD NNYYEYHEF LQTTKSYKF DLSIFGIKC SIIDKIMIL LNKLK
- >TC001812 RA
atggaagat ctttatgag aaaacaata gaaagaagc gagcaatta aaaaaagct ggttataac ttaatagaa atgtgggaa tgtgagtgg acgaggtca aaagaatat aagaaagaa atgaaacaa atcgaagaa gaaattaag gaattggaa gaacttaat cctagaaac gcattcttt ggaggaaga acgaatgca acaaaattg cgcgttaag gggaagaaa atgaaatat atagatatt tgcagttta tatccaaca gtacaatgt tacgatgat tatccagtt ggacatcct actaaaata ttcaaacct cgtacgtat aatacagat tggtatggt ttaataaaa tgcgcaatt ttaccgcca agaggattg tatcacccc gttttacct gttaagaat aaaaccaaa actggtgat gaaaaacta acatttcca ttatgtcaa ttgtgtgca aaattaaat aatcaaaaa gataaatgt tctcatacg gaatctcaa agaattatc agaggaacc tggtgtacc aacgaagta gaaaaagca attgaaaaa ggctataaa ataataagt attgatgaa gtttggcat tttgaaaaa aaatcgagt tatttgttt aaaggatat gtaaaagca tttatgaaa ataaagtta gagacaagc ccttggcag gatgatttt gaatcagaa gaagaatat agaaaagct gtaaaagaa attctaggt attgaattg ggtaaaata gaaaataat cctggaaag agagctgtt gcgaaaata tgcttaaat tccttatgg ggtaaattt gggcaaaga caaaatatg ggtgcaaca gaatatgtt acagatgtt aaaagattt tatgaaatt ttattagat gacagactt gataatata catatcaat gaaataaat gaaaatatg ttacaaatt gattataag ttaaaagat tgctatgta gaaaataat ttcaatacc aatatattc atagctgct tttacaact gcaaatgct agactaaga ttgtacgaa atgttagat aaattagga aacagcgta gcatattat gatacagat agtattgta tatatagat gatggaaaa aacactgtt aaaacaggc tgtctattg ggagattgg acagacgaa ttagatgat gttaatatt cggacgaat gaatcgaag attcagagt tcagcaaag ccttacgat gaatcgaag attcagagt tcagcaaag ccttacgat gaatcgaag attcagagt tcagcaaag ccttacgat gaatcgaag attcagagt tatttagaa caatttggc tatttcgcg tcagcgtct acaaaactt ggcaatgcc tcgcatgct gaatcatta atagaaata aatgaagca ttaattaga tttcaagaa tattacaat ttacctgtt gatggaaca ttaaatcaa gaaacactt gatttgatt aaacagccg agatgtgga aataaagat aatccaaca gcttataga gttcattat caaaaatgg aataaaaca aatcttaaa tggtatttt tcgttagct acaaacgag atgaaagaa ttagcacaa aaagccttt gatcaatgg gaatctgtt tcaaattta aagtttgag tactggcac agccagccc agcgccgta ggcgagtac aatagtgtt aaaccggac atacttatt tcattttct aatacgctg tttcaacat aatcataat tcaaggtgt caaaaagga atatgttct agcagtttt gacggaaaa ggtaatgtc ttagcacat ggcttcttt ccaaataat gatgaatgt ctaggaatt cattttgat aaatcagaa aattggtat tttggtgaa tcaagtaat acacctgat gatcaaact aatttttat acagtatta ttacacgaa atcggacat acattaggt attgagcac tctgccaat aataattca attatgtac gcatattat aaaggtgat attgataaa ttaactcga gacgatatg tgggcaatt caatattta tacggaata cctgaacgg tcaaaatat gaatcaata ccaactaca actaccaca actactaca tctaccaca actaaaaaa cctattact ttgactcct agaaagact gattcatta cctgattta tgcagctta agtgaaact attaacaca tttttaata gcaaatcat aaattatat attttctac aagaaatat gtctggatt gttaattta aaagacatg agctatgat aataaacca aaattaatt acagattat ttaactttt ttaccagat aattttcaa gaaatatca caaatttat cagagacca gatggaact attttgtta acaacaaac aacttatat tatattatt gattttcct aatttcgtt gttaaaaac ggttataat ggaagatct atcacaagt ttgggtgtt cctagtgga aagaaaatt aatgcaata tttagtact tattctggt caaacttat atattttat gacaacaca atgtttata caatttgat gaatgtctg cttagacct aagaaacat ggtatgata gctgaattg ttttctagt attccttct aatgttgat aaagccttt agatatatt aatggaaaa atttatttc tttaaagac aataattac tacgagtat catgaattt ttgcaaacg acaaaatcg tataaattt gatttatct atttttgga attaaatgt agtattata gacaagatt atgatttta ctaaataaa cttaaataa
- >TC001812 RA
atggaagat ctttatgag aaaacaata gaaagaagc gagcaatta aaaaaagct ggttataac ttaatagaa atgtgggaa tgtgagtgg acgaggtca aaagaatat aagaaagaa atgaaacaa atcgaagaa gaaattaag gaattggaa gaacttaat cctagaaac gcattcttt ggaggaaga acgaatgca acaaaattg cgcgttaag gggaagaaa atgaaatat atagatatt tgcagttta tatccaaca gtacaatgt tacgatgat tatccagtt ggacatcct actaaaata ttcaaacct cgtacgtat aatacagat tggtatggt ttaataaaa tgcgcaatt ttaccgcca agaggattg tatcacccc gttttacct gttaagaat aaaaccaaa actggtgat gaaaaacta acatttcca ttatgtcaa ttgtgtgca aaattaaat aatcaaaaa gataaatgt tctcatacg gaatctcaa agaattatc agaggaacc tggtgtacc aacgaagta gaaaaagca attgaaaaa ggctataaa ataataagt attgatgaa gtttggcat tttgaaaaa aaatcgagt tatttgttt aaaggatat gtaaaagca tttatgaaa ataaagtta gagacaagc ccttggcag gatgatttt gaatcagaa gaagaatat agaaaagct gtaaaagaa attctaggt attgaattg ggtaaaata gaaaataat cctggaaag agagctgtt gcgaaaata tgcttaaat tccttatgg ggtaaattt gggcaaaga caaaatatg ggtgcaaca gaatatgtt acagatgtt aaaagattt tatgaaatt ttattagat gacagactt gataatata catatcaat gaaataaat gaaaatatg ttacaaatt gattataag ttaaaagat tgctatgta gaaaataat ttcaatacc aatatattc atagctgct tttacaact gcaaatgct agactaaga ttgtacgaa atgttagat aaattagga aacagcgta gcatattat gatacagat agtattgta tatatagat gatggaaaa aacactgtt aaaacaggc tgtctattg ggagattgg acagacgaa ttagatgat gttaatatt cggacgaat gaatcgaag attcagagt tcagcaaag ccttacgat gaatcgaag attcagagt tcagcaaag ccttacgat gaatcgaag attcagagt tcagcaaag ccttacgat gaatcgaag attcagagt tatttagaa caatttggc tatttcgcg tcagcgtct acaaaactt ggcaatgcc tcgcatgct gaatcatta atagaaata aatgaagca ttaattaga tttcaagaa tattacaat ttacctgtt gatggaaca ttaaatcaa gaaacactt gatttgatt aaacagccg agatgtgga aataaagat aatccaaca gcttataga gttcattat caaaaatgg aataaaaca aatcttaaa tggtatttt tcgttagct acaaacgag atgaaagaa ttagcacaa aaagccttt gatcaatgg gaatctgtt tcaaattta aagtttgag tactggcac agccagccc agcgccgta ggcgagtac aatagtgtt aaaccggac atacttatt tcattttct aatacgctg tttcaacat aatcataat tcaaggtgt caaaaagga atatgttct agcagtttt gacggaaaa ggtaatgtc ttagcacat ggcttcttt ccaaataat gatgaatgt ctaggaatt cattttgat aaatcagaa aattggtat tttggtgaa tcaagtaat acacctgat gatcaaact aatttttat acagtatta ttacacgaa atcggacat acattaggt attgagcac tctgccaat aataattca attatgtac gcatattat aaaggtgat attgataaa ttaactcga gacgatatg tgggcaatt caatattta tacggaata cctgaacgg tcaaaatat gaatcaata ccaactaca actaccaca actactaca tctaccaca actaaaaaa cctattact ttgactcct agaaagact gattcatta cctgattta tgcagctta agtgaaact attaacaca tttttaata gcaaatcat aaattatat attttctac aagaaatat gtctggatt gttaattta aaagacatg agctatgat aataaacca aaattaatt acagattat ttaactttt ttaccagat aattttcaa gaaatatca caaatttat cagagacca gatggaact attttgtta acaacaaac aacttatat tatattatt gattttcct aatttcgtt gttaaaaac ggttataat ggaagatct atcacaagt ttgggtgtt cctagtgga aagaaaatt aatgcaata tttagtact tattctggt caaacttat atattttat gacaacaca atgtttata caatttgat gaatgtctg cttagacct aagaaacat ggtatgata gctgaattg ttttctagt attccttct aatgttgat aaagccttt agatatatt aatggaaaa atttatttc tttaaagac aataattac tacgagtat catgaattt ttgcaaacg acaaaatcg tataaattt gatttatct atttttgga attaaatgt agtattata gacaagatt atgatttta ctaaataaa cttaaataa
