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Gene information
General information
TC001812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450214.1: 654..6744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001812
    iBeetle screen iB_05963

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001812 RA
      MEDLYEKTI ERSEQLKKA GYNLIEMWE CEWTRSKEY 
KKEMKQIEE EIKELEELN PRNAFFGGR TNATKLRVK 
GKKMKYIDI CSLYPTVQC YDDYPVGHP TKIFKPRTY 
NTDWYGLIK CAILPPRGL YHPVLPVKN KTKTGDEKL 
TFPLCQLCA KLNNQKDKC SHTESQRII RGTWCTNEV 
EKAIEKGYK IISIDEVWH FEKKSSYLF KGYVKAFMK 
IKLETSPWQ DDFESEEEY RKAVKEILG IELGKIENN 
PGKRAVAKI CLNSLWGKF GQRQNMGAT EYVTDVKRF 
YEILLDDRL DNIHINEIN ENMLQIDYK LKDCYVENN 
FNTNIFIAA FTTANARLR LYEMLDKLG NSVAYYDTD 
SIVYIDDGK NTVKTGCLL GDWTDELDD VNIRTNESK 
IQSSAKPYD ESKIQSSAK PYDESKIQS SAKPYDESK 
IQSYLEQFG YFASASTKL GNASHAESL IEINEALIR 
FQEYYNLPV DGTLNQETL DLIKQPRCG NKDNPTAYR 
VHYQKWNKT NLKWYFSLA TNEMKELAQ KAFDQWESV 
SNLKFEYWH SQPSAVGEY NSVKPDILI SFSNTLFQH 
NHNSRCQKG ICSSSFDGK GNVLAHGFF PNNDECLGI 
HFDKSENWY FGESSNTPD DQTNFYTVL LHEIGHTLG 
IEHSANNNS IMYAYYKGD IDKLTRDDM WAIQYLYGI 
PERSKYESI PTTTTTTTT STTTKKPIT LTPRKTDSL 
PDLCSLSET INTFLIANH KLYIFYKKY VWIVNLKDM 
SYDNKPKLI TDYLTFLPD NFQEISQIY QRPDGTILL 
TTNNLYYII DFPNFVVKN GYNGRSITS LGVPSGKKI 
NAIFSTYSG QTYIFYDNT MFIQFDECL LRPKKHGMI 
AELFSSIPS NVDKAFRYI NGKIYFFKD NNYYEYHEF 
LQTTKSYKF DLSIFGIKC SIIDKIMIL LNKLK
    CDS sequence
    • >TC001812 RA
      atggaagat ctttatgag aaaacaata gaaagaagc 
gagcaatta aaaaaagct ggttataac ttaatagaa 
atgtgggaa tgtgagtgg acgaggtca aaagaatat 
aagaaagaa atgaaacaa atcgaagaa gaaattaag 
gaattggaa gaacttaat cctagaaac gcattcttt 
ggaggaaga acgaatgca acaaaattg cgcgttaag 
gggaagaaa atgaaatat atagatatt tgcagttta 
tatccaaca gtacaatgt tacgatgat tatccagtt 
ggacatcct actaaaata ttcaaacct cgtacgtat 
aatacagat tggtatggt ttaataaaa tgcgcaatt 
ttaccgcca agaggattg tatcacccc gttttacct 
gttaagaat aaaaccaaa actggtgat gaaaaacta 
acatttcca ttatgtcaa ttgtgtgca aaattaaat 
aatcaaaaa gataaatgt tctcatacg gaatctcaa 
agaattatc agaggaacc tggtgtacc aacgaagta 
gaaaaagca attgaaaaa ggctataaa ataataagt 
attgatgaa gtttggcat tttgaaaaa aaatcgagt 
tatttgttt aaaggatat gtaaaagca tttatgaaa 
ataaagtta gagacaagc ccttggcag gatgatttt 
gaatcagaa gaagaatat agaaaagct gtaaaagaa 
attctaggt attgaattg ggtaaaata gaaaataat 
cctggaaag agagctgtt gcgaaaata tgcttaaat 
tccttatgg ggtaaattt gggcaaaga caaaatatg 
ggtgcaaca gaatatgtt acagatgtt aaaagattt 
tatgaaatt ttattagat gacagactt gataatata 
catatcaat gaaataaat gaaaatatg ttacaaatt 
gattataag ttaaaagat tgctatgta gaaaataat 
ttcaatacc aatatattc atagctgct tttacaact 
gcaaatgct agactaaga ttgtacgaa atgttagat 
aaattagga aacagcgta gcatattat gatacagat 
agtattgta tatatagat gatggaaaa aacactgtt 
aaaacaggc tgtctattg ggagattgg acagacgaa 
ttagatgat gttaatatt cggacgaat gaatcgaag 
attcagagt tcagcaaag ccttacgat gaatcgaag 
attcagagt tcagcaaag ccttacgat gaatcgaag 
attcagagt tcagcaaag ccttacgat gaatcgaag 
attcagagt tatttagaa caatttggc tatttcgcg 
tcagcgtct acaaaactt ggcaatgcc tcgcatgct 
gaatcatta atagaaata aatgaagca ttaattaga 
tttcaagaa tattacaat ttacctgtt gatggaaca 
ttaaatcaa gaaacactt gatttgatt aaacagccg 
agatgtgga aataaagat aatccaaca gcttataga 
gttcattat caaaaatgg aataaaaca aatcttaaa 
tggtatttt tcgttagct acaaacgag atgaaagaa 
ttagcacaa aaagccttt gatcaatgg gaatctgtt 
tcaaattta aagtttgag tactggcac agccagccc 
agcgccgta ggcgagtac aatagtgtt aaaccggac 
atacttatt tcattttct aatacgctg tttcaacat 
aatcataat tcaaggtgt caaaaagga atatgttct 
agcagtttt gacggaaaa ggtaatgtc ttagcacat 
ggcttcttt ccaaataat gatgaatgt ctaggaatt 
cattttgat aaatcagaa aattggtat tttggtgaa 
tcaagtaat acacctgat gatcaaact aatttttat 
acagtatta ttacacgaa atcggacat acattaggt 
attgagcac tctgccaat aataattca attatgtac 
gcatattat aaaggtgat attgataaa ttaactcga 
gacgatatg tgggcaatt caatattta tacggaata 
cctgaacgg tcaaaatat gaatcaata ccaactaca 
actaccaca actactaca tctaccaca actaaaaaa 
cctattact ttgactcct agaaagact gattcatta 
cctgattta tgcagctta agtgaaact attaacaca 
tttttaata gcaaatcat aaattatat attttctac 
aagaaatat gtctggatt gttaattta aaagacatg 
agctatgat aataaacca aaattaatt acagattat 
ttaactttt ttaccagat aattttcaa gaaatatca 
caaatttat cagagacca gatggaact attttgtta 
acaacaaac aacttatat tatattatt gattttcct 
aatttcgtt gttaaaaac ggttataat ggaagatct 
atcacaagt ttgggtgtt cctagtgga aagaaaatt 
aatgcaata tttagtact tattctggt caaacttat 
atattttat gacaacaca atgtttata caatttgat 
gaatgtctg cttagacct aagaaacat ggtatgata 
gctgaattg ttttctagt attccttct aatgttgat 
aaagccttt agatatatt aatggaaaa atttatttc 
tttaaagac aataattac tacgagtat catgaattt 
ttgcaaacg acaaaatcg tataaattt gatttatct 
atttttgga attaaatgt agtattata gacaagatt 
atgatttta ctaaataaa cttaaataa
    mRNA sequence
    • >TC001812 RA
      atggaagat ctttatgag aaaacaata gaaagaagc 
gagcaatta aaaaaagct ggttataac ttaatagaa 
atgtgggaa tgtgagtgg acgaggtca aaagaatat 
aagaaagaa atgaaacaa atcgaagaa gaaattaag 
gaattggaa gaacttaat cctagaaac gcattcttt 
ggaggaaga acgaatgca acaaaattg cgcgttaag 
gggaagaaa atgaaatat atagatatt tgcagttta 
tatccaaca gtacaatgt tacgatgat tatccagtt 
ggacatcct actaaaata ttcaaacct cgtacgtat 
aatacagat tggtatggt ttaataaaa tgcgcaatt 
ttaccgcca agaggattg tatcacccc gttttacct 
gttaagaat aaaaccaaa actggtgat gaaaaacta 
acatttcca ttatgtcaa ttgtgtgca aaattaaat 
aatcaaaaa gataaatgt tctcatacg gaatctcaa 
agaattatc agaggaacc tggtgtacc aacgaagta 
gaaaaagca attgaaaaa ggctataaa ataataagt 
attgatgaa gtttggcat tttgaaaaa aaatcgagt 
tatttgttt aaaggatat gtaaaagca tttatgaaa 
ataaagtta gagacaagc ccttggcag gatgatttt 
gaatcagaa gaagaatat agaaaagct gtaaaagaa 
attctaggt attgaattg ggtaaaata gaaaataat 
cctggaaag agagctgtt gcgaaaata tgcttaaat 
tccttatgg ggtaaattt gggcaaaga caaaatatg 
ggtgcaaca gaatatgtt acagatgtt aaaagattt 
tatgaaatt ttattagat gacagactt gataatata 
catatcaat gaaataaat gaaaatatg ttacaaatt 
gattataag ttaaaagat tgctatgta gaaaataat 
ttcaatacc aatatattc atagctgct tttacaact 
gcaaatgct agactaaga ttgtacgaa atgttagat 
aaattagga aacagcgta gcatattat gatacagat 
agtattgta tatatagat gatggaaaa aacactgtt 
aaaacaggc tgtctattg ggagattgg acagacgaa 
ttagatgat gttaatatt cggacgaat gaatcgaag 
attcagagt tcagcaaag ccttacgat gaatcgaag 
attcagagt tcagcaaag ccttacgat gaatcgaag 
attcagagt tcagcaaag ccttacgat gaatcgaag 
attcagagt tatttagaa caatttggc tatttcgcg 
tcagcgtct acaaaactt ggcaatgcc tcgcatgct 
gaatcatta atagaaata aatgaagca ttaattaga 
tttcaagaa tattacaat ttacctgtt gatggaaca 
ttaaatcaa gaaacactt gatttgatt aaacagccg 
agatgtgga aataaagat aatccaaca gcttataga 
gttcattat caaaaatgg aataaaaca aatcttaaa 
tggtatttt tcgttagct acaaacgag atgaaagaa 
ttagcacaa aaagccttt gatcaatgg gaatctgtt 
tcaaattta aagtttgag tactggcac agccagccc 
agcgccgta ggcgagtac aatagtgtt aaaccggac 
atacttatt tcattttct aatacgctg tttcaacat 
aatcataat tcaaggtgt caaaaagga atatgttct 
agcagtttt gacggaaaa ggtaatgtc ttagcacat 
ggcttcttt ccaaataat gatgaatgt ctaggaatt 
cattttgat aaatcagaa aattggtat tttggtgaa 
tcaagtaat acacctgat gatcaaact aatttttat 
acagtatta ttacacgaa atcggacat acattaggt 
attgagcac tctgccaat aataattca attatgtac 
gcatattat aaaggtgat attgataaa ttaactcga 
gacgatatg tgggcaatt caatattta tacggaata 
cctgaacgg tcaaaatat gaatcaata ccaactaca 
actaccaca actactaca tctaccaca actaaaaaa 
cctattact ttgactcct agaaagact gattcatta 
cctgattta tgcagctta agtgaaact attaacaca 
tttttaata gcaaatcat aaattatat attttctac 
aagaaatat gtctggatt gttaattta aaagacatg 
agctatgat aataaacca aaattaatt acagattat 
ttaactttt ttaccagat aattttcaa gaaatatca 
caaatttat cagagacca gatggaact attttgtta 
acaacaaac aacttatat tatattatt gattttcct 
aatttcgtt gttaaaaac ggttataat ggaagatct 
atcacaagt ttgggtgtt cctagtgga aagaaaatt 
aatgcaata tttagtact tattctggt caaacttat 
atattttat gacaacaca atgtttata caatttgat 
gaatgtctg cttagacct aagaaacat ggtatgata 
gctgaattg ttttctagt attccttct aatgttgat 
aaagccttt agatatatt aatggaaaa atttatttc 
tttaaagac aataattac tacgagtat catgaattt 
ttgcaaacg acaaaatcg tataaattt gatttatct 
atttttgga attaaatgt agtattata gacaagatt 
atgatttta ctaaataaa cttaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY