Status
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Gene information
General information
TC001812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450214.1: 654..6744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001812
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05963

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001812 RA
      MEDLYEKTI ERSEQLKKA GYNLIEMWE CEWTRSKEY 
      KKEMKQIEE EIKELEELN PRNAFFGGR TNATKLRVK 
      GKKMKYIDI CSLYPTVQC YDDYPVGHP TKIFKPRTY 
      NTDWYGLIK CAILPPRGL YHPVLPVKN KTKTGDEKL 
      TFPLCQLCA KLNNQKDKC SHTESQRII RGTWCTNEV 
      EKAIEKGYK IISIDEVWH FEKKSSYLF KGYVKAFMK 
      IKLETSPWQ DDFESEEEY RKAVKEILG IELGKIENN 
      PGKRAVAKI CLNSLWGKF GQRQNMGAT EYVTDVKRF 
      YEILLDDRL DNIHINEIN ENMLQIDYK LKDCYVENN 
      FNTNIFIAA FTTANARLR LYEMLDKLG NSVAYYDTD 
      SIVYIDDGK NTVKTGCLL GDWTDELDD VNIRTNESK 
      IQSSAKPYD ESKIQSSAK PYDESKIQS SAKPYDESK 
      IQSYLEQFG YFASASTKL GNASHAESL IEINEALIR 
      FQEYYNLPV DGTLNQETL DLIKQPRCG NKDNPTAYR 
      VHYQKWNKT NLKWYFSLA TNEMKELAQ KAFDQWESV 
      SNLKFEYWH SQPSAVGEY NSVKPDILI SFSNTLFQH 
      NHNSRCQKG ICSSSFDGK GNVLAHGFF PNNDECLGI 
      HFDKSENWY FGESSNTPD DQTNFYTVL LHEIGHTLG 
      IEHSANNNS IMYAYYKGD IDKLTRDDM WAIQYLYGI 
      PERSKYESI PTTTTTTTT STTTKKPIT LTPRKTDSL 
      PDLCSLSET INTFLIANH KLYIFYKKY VWIVNLKDM 
      SYDNKPKLI TDYLTFLPD NFQEISQIY QRPDGTILL 
      TTNNLYYII DFPNFVVKN GYNGRSITS LGVPSGKKI 
      NAIFSTYSG QTYIFYDNT MFIQFDECL LRPKKHGMI 
      AELFSSIPS NVDKAFRYI NGKIYFFKD NNYYEYHEF 
      LQTTKSYKF DLSIFGIKC SIIDKIMIL LNKLK
    CDS sequence
    • >TC001812 RA
      atggaagat ctttatgag aaaacaata gaaagaagc 
      gagcaatta aaaaaagct ggttataac ttaatagaa 
      atgtgggaa tgtgagtgg acgaggtca aaagaatat 
      aagaaagaa atgaaacaa atcgaagaa gaaattaag 
      gaattggaa gaacttaat cctagaaac gcattcttt 
      ggaggaaga acgaatgca acaaaattg cgcgttaag 
      gggaagaaa atgaaatat atagatatt tgcagttta 
      tatccaaca gtacaatgt tacgatgat tatccagtt 
      ggacatcct actaaaata ttcaaacct cgtacgtat 
      aatacagat tggtatggt ttaataaaa tgcgcaatt 
      ttaccgcca agaggattg tatcacccc gttttacct 
      gttaagaat aaaaccaaa actggtgat gaaaaacta 
      acatttcca ttatgtcaa ttgtgtgca aaattaaat 
      aatcaaaaa gataaatgt tctcatacg gaatctcaa 
      agaattatc agaggaacc tggtgtacc aacgaagta 
      gaaaaagca attgaaaaa ggctataaa ataataagt 
      attgatgaa gtttggcat tttgaaaaa aaatcgagt 
      tatttgttt aaaggatat gtaaaagca tttatgaaa 
      ataaagtta gagacaagc ccttggcag gatgatttt 
      gaatcagaa gaagaatat agaaaagct gtaaaagaa 
      attctaggt attgaattg ggtaaaata gaaaataat 
      cctggaaag agagctgtt gcgaaaata tgcttaaat 
      tccttatgg ggtaaattt gggcaaaga caaaatatg 
      ggtgcaaca gaatatgtt acagatgtt aaaagattt 
      tatgaaatt ttattagat gacagactt gataatata 
      catatcaat gaaataaat gaaaatatg ttacaaatt 
      gattataag ttaaaagat tgctatgta gaaaataat 
      ttcaatacc aatatattc atagctgct tttacaact 
      gcaaatgct agactaaga ttgtacgaa atgttagat 
      aaattagga aacagcgta gcatattat gatacagat 
      agtattgta tatatagat gatggaaaa aacactgtt 
      aaaacaggc tgtctattg ggagattgg acagacgaa 
      ttagatgat gttaatatt cggacgaat gaatcgaag 
      attcagagt tcagcaaag ccttacgat gaatcgaag 
      attcagagt tcagcaaag ccttacgat gaatcgaag 
      attcagagt tcagcaaag ccttacgat gaatcgaag 
      attcagagt tatttagaa caatttggc tatttcgcg 
      tcagcgtct acaaaactt ggcaatgcc tcgcatgct 
      gaatcatta atagaaata aatgaagca ttaattaga 
      tttcaagaa tattacaat ttacctgtt gatggaaca 
      ttaaatcaa gaaacactt gatttgatt aaacagccg 
      agatgtgga aataaagat aatccaaca gcttataga 
      gttcattat caaaaatgg aataaaaca aatcttaaa 
      tggtatttt tcgttagct acaaacgag atgaaagaa 
      ttagcacaa aaagccttt gatcaatgg gaatctgtt 
      tcaaattta aagtttgag tactggcac agccagccc 
      agcgccgta ggcgagtac aatagtgtt aaaccggac 
      atacttatt tcattttct aatacgctg tttcaacat 
      aatcataat tcaaggtgt caaaaagga atatgttct 
      agcagtttt gacggaaaa ggtaatgtc ttagcacat 
      ggcttcttt ccaaataat gatgaatgt ctaggaatt 
      cattttgat aaatcagaa aattggtat tttggtgaa 
      tcaagtaat acacctgat gatcaaact aatttttat 
      acagtatta ttacacgaa atcggacat acattaggt 
      attgagcac tctgccaat aataattca attatgtac 
      gcatattat aaaggtgat attgataaa ttaactcga 
      gacgatatg tgggcaatt caatattta tacggaata 
      cctgaacgg tcaaaatat gaatcaata ccaactaca 
      actaccaca actactaca tctaccaca actaaaaaa 
      cctattact ttgactcct agaaagact gattcatta 
      cctgattta tgcagctta agtgaaact attaacaca 
      tttttaata gcaaatcat aaattatat attttctac 
      aagaaatat gtctggatt gttaattta aaagacatg 
      agctatgat aataaacca aaattaatt acagattat 
      ttaactttt ttaccagat aattttcaa gaaatatca 
      caaatttat cagagacca gatggaact attttgtta 
      acaacaaac aacttatat tatattatt gattttcct 
      aatttcgtt gttaaaaac ggttataat ggaagatct 
      atcacaagt ttgggtgtt cctagtgga aagaaaatt 
      aatgcaata tttagtact tattctggt caaacttat 
      atattttat gacaacaca atgtttata caatttgat 
      gaatgtctg cttagacct aagaaacat ggtatgata 
      gctgaattg ttttctagt attccttct aatgttgat 
      aaagccttt agatatatt aatggaaaa atttatttc 
      tttaaagac aataattac tacgagtat catgaattt 
      ttgcaaacg acaaaatcg tataaattt gatttatct 
      atttttgga attaaatgt agtattata gacaagatt 
      atgatttta ctaaataaa cttaaataa
    mRNA sequence
    • >TC001812 RA
      atggaagat ctttatgag aaaacaata gaaagaagc 
      gagcaatta aaaaaagct ggttataac ttaatagaa 
      atgtgggaa tgtgagtgg acgaggtca aaagaatat 
      aagaaagaa atgaaacaa atcgaagaa gaaattaag 
      gaattggaa gaacttaat cctagaaac gcattcttt 
      ggaggaaga acgaatgca acaaaattg cgcgttaag 
      gggaagaaa atgaaatat atagatatt tgcagttta 
      tatccaaca gtacaatgt tacgatgat tatccagtt 
      ggacatcct actaaaata ttcaaacct cgtacgtat 
      aatacagat tggtatggt ttaataaaa tgcgcaatt 
      ttaccgcca agaggattg tatcacccc gttttacct 
      gttaagaat aaaaccaaa actggtgat gaaaaacta 
      acatttcca ttatgtcaa ttgtgtgca aaattaaat 
      aatcaaaaa gataaatgt tctcatacg gaatctcaa 
      agaattatc agaggaacc tggtgtacc aacgaagta 
      gaaaaagca attgaaaaa ggctataaa ataataagt 
      attgatgaa gtttggcat tttgaaaaa aaatcgagt 
      tatttgttt aaaggatat gtaaaagca tttatgaaa 
      ataaagtta gagacaagc ccttggcag gatgatttt 
      gaatcagaa gaagaatat agaaaagct gtaaaagaa 
      attctaggt attgaattg ggtaaaata gaaaataat 
      cctggaaag agagctgtt gcgaaaata tgcttaaat 
      tccttatgg ggtaaattt gggcaaaga caaaatatg 
      ggtgcaaca gaatatgtt acagatgtt aaaagattt 
      tatgaaatt ttattagat gacagactt gataatata 
      catatcaat gaaataaat gaaaatatg ttacaaatt 
      gattataag ttaaaagat tgctatgta gaaaataat 
      ttcaatacc aatatattc atagctgct tttacaact 
      gcaaatgct agactaaga ttgtacgaa atgttagat 
      aaattagga aacagcgta gcatattat gatacagat 
      agtattgta tatatagat gatggaaaa aacactgtt 
      aaaacaggc tgtctattg ggagattgg acagacgaa 
      ttagatgat gttaatatt cggacgaat gaatcgaag 
      attcagagt tcagcaaag ccttacgat gaatcgaag 
      attcagagt tcagcaaag ccttacgat gaatcgaag 
      attcagagt tcagcaaag ccttacgat gaatcgaag 
      attcagagt tatttagaa caatttggc tatttcgcg 
      tcagcgtct acaaaactt ggcaatgcc tcgcatgct 
      gaatcatta atagaaata aatgaagca ttaattaga 
      tttcaagaa tattacaat ttacctgtt gatggaaca 
      ttaaatcaa gaaacactt gatttgatt aaacagccg 
      agatgtgga aataaagat aatccaaca gcttataga 
      gttcattat caaaaatgg aataaaaca aatcttaaa 
      tggtatttt tcgttagct acaaacgag atgaaagaa 
      ttagcacaa aaagccttt gatcaatgg gaatctgtt 
      tcaaattta aagtttgag tactggcac agccagccc 
      agcgccgta ggcgagtac aatagtgtt aaaccggac 
      atacttatt tcattttct aatacgctg tttcaacat 
      aatcataat tcaaggtgt caaaaagga atatgttct 
      agcagtttt gacggaaaa ggtaatgtc ttagcacat 
      ggcttcttt ccaaataat gatgaatgt ctaggaatt 
      cattttgat aaatcagaa aattggtat tttggtgaa 
      tcaagtaat acacctgat gatcaaact aatttttat 
      acagtatta ttacacgaa atcggacat acattaggt 
      attgagcac tctgccaat aataattca attatgtac 
      gcatattat aaaggtgat attgataaa ttaactcga 
      gacgatatg tgggcaatt caatattta tacggaata 
      cctgaacgg tcaaaatat gaatcaata ccaactaca 
      actaccaca actactaca tctaccaca actaaaaaa 
      cctattact ttgactcct agaaagact gattcatta 
      cctgattta tgcagctta agtgaaact attaacaca 
      tttttaata gcaaatcat aaattatat attttctac 
      aagaaatat gtctggatt gttaattta aaagacatg 
      agctatgat aataaacca aaattaatt acagattat 
      ttaactttt ttaccagat aattttcaa gaaatatca 
      caaatttat cagagacca gatggaact attttgtta 
      acaacaaac aacttatat tatattatt gattttcct 
      aatttcgtt gttaaaaac ggttataat ggaagatct 
      atcacaagt ttgggtgtt cctagtgga aagaaaatt 
      aatgcaata tttagtact tattctggt caaacttat 
      atattttat gacaacaca atgtttata caatttgat 
      gaatgtctg cttagacct aagaaacat ggtatgata 
      gctgaattg ttttctagt attccttct aatgttgat 
      aaagccttt agatatatt aatggaaaa atttatttc 
      tttaaagac aataattac tacgagtat catgaattt 
      ttgcaaacg acaaaatcg tataaattt gatttatct 
      atttttgga attaaatgt agtattata gacaagatt 
      atgatttta ctaaataaa cttaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY