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Gene information
General information
TC016127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451646.1: 224078..225353
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016127
    iBeetle screen iB_05962

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016127 RA
      MGEDFGEGG PPVPGEGVR QFRPPTAPS IPEVIKEKI 
      LTFEEASKC LNTLGKDET GSRYAYLMI TATNRKLTD 
      VSIILRFKH VLFLDLSGN YLTTDALTV LTEMPFLIL 
      LKAERNRVD SAALKPMPY LQVLALNQN QIKQVGNID 
      QPLLDCLEM NYNDIYSTE FETANLKQL KQLEMRSNL 
      LFEISGFYP PNLRKLYMA ANKITTINS PEFAKLSRL 
      ETLHLRENS IQNLDGFSE AQTSLKYLN LRNNKIEKF 
      KEFRKLQCL PNLETLIVT GNPLPGSEV PQMGGEGGG 
      GGVFGEGGK IRDPVVIPL LVLLPKLKR INKTVITME 
      DRIDAEDME NDVIEALLR DDETAQDTD RETTTTDYK 
      QESDLDG
    CDS sequence
    • >TC016127 RA
      atgggcgaa gatttcggt gaaggtgga cctccagtt 
      cctggcgaa ggcgtccgt caattccgt cccccaacc 
      gccccctca atccccgaa gtaatcaaa gagaaaata 
      ctaacattc gaagaagcc tccaaatgc ctcaacacc 
      ctcggcaaa gatgaaacc ggaagccgc tacgcctac 
      ttgatgata acagcaaca aatcgcaaa ctaaccgat 
      gtctccata atcctccgc ttcaaacac gtgctattc 
      ctcgatctc agcggcaac tacctcacc actgacgcc 
      ctcactgtc ctcactgaa atgcccttc ctcatactc 
      ctcaaagcc gagaggaac cgagtcgat tcagccgcc 
      ctcaaaccc atgccctac ctccaggtc ttagccctc 
      aaccaaaac caaatcaaa caggtggga aacatcgac 
      caacccctg cttgactgc ctcgaaatg aactacaac 
      gacatctac agcacggaa ttcgaaact gccaatttg 
      aagcaatta aaacaactg gagatgaga agtaatctc 
      ttgtttgaa atttcgggg ttttaccca cctaaccta 
      cggaaactc tacatggct gccaataaa atcacgacg 
      ataaattcg ccagaattt gcaaaactt tcgcgtctg 
      gaaacactt catttgcga gagaacagc atccagaat 
      ttggacggt ttctccgag gctcaaact agtctcaaa 
      tatctcaat ttgcggaac aataaaatc gaaaaattt 
      aaggagttt cgaaaattg cagtgttta ccaaatttg 
      gaaactttg atcgtcact ggaaatccg ctcccaggg 
      agtgaagtc ccacaaatg ggtggtgaa ggtggtggt 
      gggggagtg tttggtgaa gggggtaag atcagggat 
      ccggtggtg attccactc ttggtgttg ttaccaaag 
      ctaaagagg attaataaa acggtgatt acaatggag 
      gataggatt gatgctgag gatatggag aatgatgtt 
      atagaggca ttgttgagg gatgatgag acggctcaa 
      gacacagat agggagacg acgactacg gattataag 
      caagagtcg gatttggat gggtga
    mRNA sequence
    • >TC016127 RA
      ggaatgaaa tttatagac attgaagtt ttgacagat 
      tttgtcaac acaacaact atctccatt tttgaaatc 
      aatagaaaa ccgtaatgg gcgaagatt tcggtgaag 
      gtggacctc cagttcctg gcgaaggcg tccgtcaat 
      tccgtcccc caaccgccc cctcaatcc ccgaagtaa 
      tcaaagaga aaatactaa cattcgaag aagcctcca 
      aatgcctca acaccctcg gcaaagatg aaaccggaa 
      gccgctacg cctacttga tgataacag caacaaatc 
      gcaaactaa ccgatgtct ccataatcc tccgcttca 
      aacacgtgc tattcctcg atctcagcg gcaactacc 
      tcaccactg acgccctca ctgtcctca ctgaaatgc 
      ccttcctca tactcctca aagccgaga ggaaccgag 
      tcgattcag ccgccctca aacccatgc cctacctcc 
      aggtcttag ccctcaacc aaaaccaaa tcaaacagg 
      tgggaaaca tcgaccaac ccctgcttg actgcctcg 
      aaatgaact acaacgaca tctacagca cggaattcg 
      aaactgcca atttgaagc aattaaaac aactggaga 
      tgagaagta atctcttgt ttgaaattt cggggtttt 
      acccaccta acctacgga aactctaca tggctgcca 
      ataaaatca cgacgataa attcgccag aatttgcaa 
      aactttcgc gtctggaaa cacttcatt tgcgagaga 
      acagcatcc agaatttgg acggtttct ccgaggctc 
      aaactagtc tcaaatatc tcaatttgc ggaacaata 
      aaatcgaaa aatttaagg agtttcgaa aattgcagt 
      gtttaccaa atttggaaa ctttgatcg tcactggaa 
      atccgctcc cagggagtg aagtcccac aaatgggtg 
      gtgaaggtg gtggtgggg gagtgtttg gtgaagggg 
      gtaagatca gggatccgg tggtgattc cactcttgg 
      tgttgttac caaagctaa agaggatta ataaaacgg 
      tgattacaa tggaggata ggattgatg ctgaggata 
      tggagaatg atgttatag aggcattgt tgagggatg 
      atgagacgg ctcaagaca cagataggg agacgacga 
      ctacggatt ataagcaag agtcggatt tggatgggt 
      gaagtattg ttttagtta aatactcac attcggcca 
      tttggtgat gaagtttca acaacgtga tctgcaata 
      aaatgaaaa tgctgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY