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Gene information
General information
TC016125 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451646.1: 165666..173502
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016125
    iBeetle screen iB_05960

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016125 RA
      MPENENLTD VIKLISDIR HGKVFNSYE IALRTEQLL 
      EKLISEGQW NNARELMQQ IKDKIRQVS QSLPLEATA 
      TNIMRHILK VIREEFDLG SEKKAEGQS LHQIVTGDQ 
      SDELDYSES HGNLRNRLL DHLTEYKVE LETSTENIA 
      AQALEHIHT NEIILTVGI SNTVEKFLK HAAKQRKFQ 
      VIVVEAAPL YRGHKMAAS LANSNIQTT VIPDSGVFA 
      MMSRVNKVI IGTHSVMAD GGLRAASGV HAIALAAKH 
      YSVPVMVLS HMYKLTPTY VCSHEKDTF NMCASPANV 
      IPYSTGPLL NKITVYNPI FDYVPPELV TLFISHQGG 
      NAPSYVYRL LSELYHPDD YEL
    CDS sequence
    • >TC016125 RA
      atgccagaa aacgaaaat ttaaccgac gttattaaa 
      ctaataagt gatattcgt catgggaag gtgttcaac 
      tcgtacgaa atagccctc agaactgag caactttta 
      gaaaaactc atctctgag gggcagtgg aacaatgca 
      cgagagttg atgcaacaa atcaaagac aaaatcaga 
      caagtctcg cagtctctc cctcttgaa gccaccgcc 
      actaacata atgcgccac attttgaaa gtaatccgt 
      gaagagttt gatttgggc tcagagaaa aaagccgag 
      ggacaatca ctacaccag attgtaaca ggtgatcaa 
      agtgacgaa cttgactat tcggagtca cacggaaac 
      ttgcgaaat cgacttttg gaccatttg accgaatat 
      aaggtcgag ttggagaca agcacggag aatatcgcg 
      gcacaagcc ttggaacat attcacaca aatgagatt 
      attttaacg gttgggatt tcaaatact gtggaaaaa 
      ttcttaaaa catgcggcc aaacagcgg aaatttcaa 
      gtgattgtc gtcgaggct gcgccttta tacaggggt 
      cataaaatg gcagcaagt cttgccaac agcaacatc 
      caaacgact gtaattcct gattctgga gtttttgca 
      atgatgtca cgtgtcaat aaagttata attggcact 
      cacagtgtt atggccgat ggaggtctc cgtgccgca 
      agtggagtt catgctatt gctctggcg gctaaacac 
      tactcagtt cctgttatg gttctctct cacatgtat 
      aaattgact ccgacctat gtctgctca catgaaaaa 
      gacaccttt aatatgtgt gcatcgcct gctaatgtt 
      attccctat tcaactgga ccgcttttg aataagatt 
      actgtttat aatccgatt tttgactac gttccacca 
      gaattggtg acgcttttt atatcacat cagggtggc 
      aacgcacca tcttacgtc taccgtctc ctctccgaa 
      ttgtaccac cctgacgac tacgaactt tga
    mRNA sequence
    • >TC016125 RA
      atgctattg acatttttc atatgttaa gttaaccta 
      caaatataa caattttaa aatatctca aaaaccgta 
      ttagaaatg ccagaaaac gaaaattta accgacgtt 
      attaaacta ataagtgat attcgtcat gggaaggtg 
      ttcaactcg tacgaaata gccctcaga actgagcaa 
      cttttagaa aaactcatc tctgagggg cagtggaac 
      aatgcacga gagttgatg caacaaatc aaagacaaa 
      atcagacaa gtctcgcag tctctccct cttgaagcc 
      accgccact aacataatg cgccacatt ttgaaagta 
      atccgtgaa gagtttgat ttgggctca gagaaaaaa 
      gccgaggga caatcacta caccagatt gtaacaggt 
      gatcaaagt gacgaactt gactattcg gagtcacac 
      ggaaacttg cgaaatcga cttttggac catttgacc 
      gaatataag gtcgagttg gagacaagc acggagaat 
      atcgcggca caagccttg gaacatatt cacacaaat 
      gagattatt ttaacggtt gggatttca aatactgtg 
      gaaaaattc ttaaaacat gcggccaaa cagcggaaa 
      tttcaagtg attgtcgtc gaggctgcg cctttatac 
      aggggtcat aaaatggca gcaagtctt gccaacagc 
      aacatccaa acgactgta attcctgat tctggagtt 
      tttgcaatg atgtcacgt gtcaataaa gttataatt 
      ggcactcac agtgttatg gccgatgga ggtctccgt 
      gccgcaagt ggagttcat gctattgct ctggcggct 
      aaacactac tcagttcct gttatggtt ctctctcac 
      atgtataaa ttgactccg acctatgtc tgctcacat 
      gaaaaagac acctttaat atgtgtgca tcgcctgct 
      aatgttatt ccctattca actggaccg cttttgaat 
      aagattact gtttataat ccgattttt gactacgtt 
      ccaccagaa ttggtgacg ctttttata tcacatcag 
      ggtggcaac gcaccatct tacgtctac cgtctcctc 
      tccgaattg taccaccct gacgactac gaactttga 
      aaccaatta aataaaacc ataaatttt atcaaaact 
      tttattttt caccattac tttacaaaa attacgctt 
      atattggct taatcttaa aatgcattc caatcaaag 
      aaaacacca aacacttta aatcctgca ccaaataat 
      aaaagcagc gcagtcctt cgggatcct tcgactgac 
      tcacatcga ccaaagacc cagttttcg aggtagtaa 
      acgaaatgt gttcatcat tgatcacaa tctcaagct 
      cctgacgac cgattctat ccggtggcg gccacaacg 
      aatcgtctt cttgcatta tctccgaat cgataataa 
      tcctcttga gctcctcca tcacgcact gatgcacat 
      aaacctcct tacggatca ttgtgtcgt tcttgtagt 
      tggaattat tggcgtaac gcaatttac catcgggac 
      ggaactcaa actcgagaa actcatgcc caaatttac 
      ctttgtggc ccacgtagt aacgtatgt aaaaatcgg 
      cagacattc taacggaat ttagggaag ccgcttgga 
      acgtttgtg gggttaggg atcacttct gtcaaattt 
      gtgacagcg ttagtgttg ccaacacaa cagacaaaa 
      tcaccagga cggcgcgct gaaaattta atttgggaa 
      gtaaaaacg cgtccagat ctagaaaaa ttcacgtct 
      agaatttgt catcaacag aaaataaaa tgacaaaac 
      tgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY