Status
Please wait ...
Gene information
General information
TC016122 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451646.1: 128646..139130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016122
    iBeetle screen iB_05959

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016122 RA
      MEGIECSGF TFYNEFDSA NLAKVVYVH PNESVIPVP 
      NSSTKSTPE VPDAEFNLW TKPDCCGTE FENGNRTWF 
      YFGVKAHSP CLLVRLNIV DLNRQGKMY SQGMAPVYR 
      TVPGKMQWE RIRDKPIYN TVDDIFTLS FKFKTPENV 
      QSITYFAFT YPFSYTDLQ KLLTNIDLK FENFTANYE 
      DDIYYHKEV VCRSLGGRD IHLLTISSY HGITPEIET 
      RLKNLFPDK NSRRPFKFV GKKVVFVSA RVHPGETPS 
      SFVFNGFLN LLLTRDDPV AIMLRRLYV FKMIPFLNP 
      DGVVLGHYR TDTRGVNLN RVYLNPILA QHPAVYAAR 
      ALIRYYHYG YEKEDVVDK SQGDATISV KVSNMSLEE 
      KEGGSVWCA KCKGKMCRR CRSAEIVKD EDENIEVNA 
      NESGLFLYI DLHGHASKK GIFMYGNHF QDLERNVEC 
      MLLPKLMSM NNHNFHFTA CNFTERNMY LRDRRDGMS 
      REGSGRVAV LKLTGLTKS YTLECNYNT GRIVNVLPA 
      TIKESHNKV HTILVPPKY TPQIFEEVG RSLGASILD 
      LTGQNPCSR IPNSEFHSI SGLKDWLRI YCANEMDET 
      RPHSKIKLR NLAGLHNQG KPSHGLLLK TRPVSAPKR 
      LANRPRPAT VPIERKENM AVSSAAAML ASQPSCSHH 
      TTTNDTNTK TFRLNKIKT KTKFKRNVS GERKIVRKQ 
      IKPTKKTIA EERKKLREI CIEAKSEEK LWKDEAGPS 
      RGATFIVHN FAKNSTFVK CKFKKHSLR RLTKSSPDA 
      GKVDKVKKR KKLKSK
    CDS sequence
    • >TC016122 RA
      atggaagga attgaatgt tcaggattc actttttac 
      aatgaattc gattcggcc aatttagct aaagttgtc 
      tacgttcac cccaatgaa tcggttatt ccggttccc 
      aacagttcg accaagtca acacctgaa gtcccagat 
      gctgagttc aatctttgg acgaagccc gattgttgt 
      gggacggag ttcgagaac gggaacagg acttggttc 
      tattttgga gtaaaagca cattcgcca tgtcttcta 
      gtacgcctc aatatagtc gatctcaat cgacaaggc 
      aaaatgtac agtcaaggt atggcaccc gtatatcgt 
      acagtccct ggaaaaatg caatgggaa cgcatacgt 
      gacaagccc atctataac accgttgac gacatattc 
      actctcagt tttaaattc aaaacccca gaaaatgtc 
      caaagtatc acatatttc gccttcact tacccattt 
      tcctacacc gatcttcaa aaactatta acaaacatt 
      gatttaaaa ttcgaaaat ttcacagcc aactatgag 
      gacgatatt tactaccat aaagaagta gtttgtcga 
      tcgttaggt ggccgcgac attcacctt ttgacaatt 
      tcttcttac catggaata acacccgaa attgaaacc 
      cgcttgaaa aatcttttt cccgataaa aattcccgt 
      cgaccgttt aaattcgtc ggtaagaaa gtcgtcttc 
      gtgtcggct cgggttcat ccaggagaa actccttcg 
      agttttgtt tttaacggt tttctgaat ctactattg 
      actcgtgac gatccggtt gcgataatg ttgagaaga 
      ttgtacgtt ttcaagatg attccgttt ttgaatcct 
      gatggtgta gttcttgga cattatagg acggataca 
      agaggtgtt aacttaaat cgagtttat ttgaatccg 
      attttggca caacatccg gcagtttat gctgcaagg 
      gctttgata aggtactat cactacggc tatgagaag 
      gaggatgtg gtggataag tcgcaagga gacgcgacg 
      attagtgtt aaagtgtcg aatatgagt cttgaggag 
      aaggaggga ggaagcgtt tggtgtgca aaatgtaaa 
      ggtaagatg tgtcgaaga tgtagaagt gccgaaatt 
      gtaaaagat gaagatgaa aatatcgaa gttaacgca 
      aacgaaagc ggactattt ctgtacata gatttgcac 
      ggccatgcg tctaaaaaa gggattttt atgtacgga 
      aatcatttt caagacttg gaacggaac gtcgaatgt 
      atgctttta ccgaaactg atgagtatg aataatcat 
      aattttcac ttcactgcc tgtaatttc accgaaagg 
      aatatgtat ctcagggac cgtcgagac ggcatgagt 
      cgagaaggc tctggccgt gtagctgta ctcaagctc 
      accggttta acaaaaagt tatactctc gaatgtaac 
      tacaacaca ggtcgaatc gtaaacgtc cttccagcg 
      accataaaa gaatctcac aataaagtc cacacgatt 
      ttggttcct ccaaaatac actccacaa atattcgaa 
      gaggtcggt agatcacta ggtgcttca attctagac 
      ctcaccggt caaaatccc tgttcacgt attcccaat 
      tccgaattt cattccatt tccggtttg aaagattgg 
      ctaaggatc tattgtgcc aatgagatg gacgagact 
      agacctcac agcaaaatc aagctcaga aacttagcc 
      ggcctccat aatcaggga aaaccctct catggttta 
      ttactgaag acgcgaccc gtttcggcg ccgaaacgt 
      ctagcaaac cgtcctaga cctgccaca gttccaatc 
      gagcgcaaa gaaaacatg gcggtgagt tcagccgcc 
      gccatgttg gcgtcacaa ccgagttgt tcacatcat 
      accaccacc aatgatact aacactaaa actttccgt 
      ttgaataaa atcaaaacg aaaactaaa ttcaaacgg 
      aacgtcagc ggtgaaaga aaaatcgtc cgcaaacag 
      ataaaacca acaaagaaa acaattgct gaagagcgg 
      aagaagttg cgcgagatt tgcatcgaa gcgaaaagc 
      gaggagaaa ttgtggaag gacgaggca gggccgagt 
      cgtggagcc acttttatc gttcataat tttgcgaaa 
      aattccact tttgtcaag tgtaagttt aaaaagcac 
      tcgttgcgg aggttgacg aaaagttcg cccgatgct 
      ggtaaggtc gacaaagtg aagaaaagg aagaaactc 
      aagtcgaaa tag
    mRNA sequence
    • >TC016122 RA
      tcgtagtaa ttattgcgt aactccgaa aggtgcttt 
      acataataa aacactacg tgtatcact atcactatt 
      tctagtgac ggtttctgt acagttttc cgtcaattt 
      ctttcagaa aaactgaat ttccatcca aatgtcact 
      tgtcatgtt tgtgtaatg tcaaaacaa atacaaaac 
      aactgtcag ctgaaaaaa tgacaagtt tataagaat 
      atttcgtgt caaaacaat tccgtgaga tattttagg 
      tactggtca aagtgttgc gggggtgac gtaattttc 
      gtgtagatt tttgatcat gggaaactt tggttactg 
      tggtatcaa gtgaattat ggaaggaat tgaatgttc 
      aggattcac tttttacaa tgaattcga ttcggccaa 
      tttagctaa agttgtcta cgttcaccc caatgaatc 
      ggttattcc ggttcccaa cagttcgac caagtcaac 
      acctgaagt cccagatgc tgagttcaa tctttggac 
      gaagcccga ttgttgtgg gacggagtt cgagaacgg 
      gaacaggac ttggttcta ttttggagt aaaagcaca 
      ttcgccatg tcttctagt acgcctcaa tatagtcga 
      tctcaatcg acaaggcaa aatgtacag tcaaggtat 
      ggcacccgt atatcgtac agtccctgg aaaaatgca 
      atgggaacg catacgtga caagcccat ctataacac 
      cgttgacga catattcac tctcagttt taaattcaa 
      aaccccaga aaatgtcca aagtatcac atatttcgc 
      cttcactta cccattttc ctacaccga tcttcaaaa 
      actattaac aaacattga tttaaaatt cgaaaattt 
      cacagccaa ctatgagga cgatattta ctaccataa 
      agaagtagt ttgtcgatc gttaggtgg ccgcgacat 
      tcacctttt gacaatttc ttcttacca tggaataac 
      acccgaaat tgaaacccg cttgaaaaa tctttttcc 
      cgataaaaa ttcccgtcg accgtttaa attcgtcgg 
      taagaaagt cgtcttcgt gtcggctcg ggttcatcc 
      aggagaaac tccttcgag ttttgtttt taacggttt 
      tctgaatct actattgac tcgtgacga tccggttgc 
      gataatgtt gagaagatt gtacgtttt caagatgat 
      tccgttttt gaatcctga tggtgtagt tcttggaca 
      ttataggac ggatacaag aggtgttaa cttaaatcg 
      agtttattt gaatccgat tttggcaca acatccggc 
      agtttatgc tgcaagggc tttgataag gtactatca 
      ctacggcta tgagaagga ggatgtggt ggataagtc 
      gcaaggaga cgcgacgat tagtgttaa agtgtcgaa 
      tatgagtct tgaggagaa ggagggagg aagcgtttg 
      gtgtgcaaa atgtaaagg taagatgtg tcgaagatg 
      tagaagtgc cgaaattgt aaaagatga agatgaaaa 
      tatcgaagt taacgcaaa cgaaagcgg actatttct 
      gtacataga tttgcacgg ccatgcgtc taaaaaagg 
      gatttttat gtacggaaa tcattttca agacttgga 
      acggaacgt cgaatgtat gcttttacc gaaactgat 
      gagtatgaa taatcataa ttttcactt cactgcctg 
      taatttcac cgaaaggaa tatgtatct cagggaccg 
      tcgagacgg catgagtcg agaaggctc tggccgtgt 
      agctgtact caagctcac cggtttaac aaaaagtta 
      tactctcga atgtaacta caacacagg tcgaatcgt 
      aaacgtcct tccagcgac cataaaaga atctcacaa 
      taaagtcca cacgatttt ggttcctcc aaaatacac 
      tccacaaat attcgaaga ggtcggtag atcactagg 
      tgcttcaat tctagacct caccggtca aaatccctg 
      ttcacgtat tcccaattc cgaatttca ttccatttc 
      cggtttgaa agattggct aaggatcta ttgtgccaa 
      tgagatgga cgagactag acctcacag caaaatcaa 
      gctcagaaa cttagccgg cctccataa tcagggaaa 
      accctctca tggtttatt actgaagac gcgacccgt 
      ttcggcgcc gaaacgtct agcaaaccg tcctagacc 
      tgccacagt tccaatcga gcgcaaaga aaacatggc 
      ggtgagttc agccgccgc catgttggc gtcacaacc 
      gagttgttc acatcatac caccaccaa tgatactaa 
      cactaaaac tttccgttt gaataaaat caaaacgaa 
      aactaaatt caaacggaa cgtcagcgg tgaaagaaa 
      aatcgtccg caaacagat aaaaccaac aaagaaaac 
      aattgctga agagcggaa gaagttgcg cgagatttg 
      catcgaagc gaaaagcga ggagaaatt gtggaagga 
      cgaggcagg gccgagtcg tggagccac ttttatcgt 
      tcataattt tgcgaaaaa ttccacttt tgtcaagtg 
      taagtttaa aaagcactc gttgcggag gttgacgaa 
      aagttcgcc cgatgctgg taaggtcga caaagtgaa 
      gaaaaggaa gaaactcaa gtcgaaata gatttctgt 
      ttgtaaatt ggtggttta taaagtgat atttttgtg 
      attttggtt gttattggt tgaggtggt gcgctctgg 
      tggtcggtt gccaaactg atcactggt gaccgcgtt 
      gtttttttt ttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY