Status
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Gene information
General information
TC016104 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451130.1: 0..4836
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC016104
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05958

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Fertility
      vitellarium shortened - Number of animals showing the phenotype: 4

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       larva partially ventralnot closed - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection

      Pupal morphology
      wings shortened - Number of animals showing the phenotype: 7

      wings orientation irregular - Number of animals showing the phenotype: 7

      Adult morphology
      flagellomeres number decreased - Number of animals showing the phenotype: 3


      club partially fused - Number of animals showing the phenotype: 3

      wings not closed - Number of animals showing the phenotype: 3


      scutellum flattened - Number of animals showing the phenotype: 3


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016104 RA
        SNDITPPST PTSLAPPTS NNERPPVQE GCVEIVYSG 
PDEDSPSQA IESDAVLEE RVAEMKEEF SNCVVPPLQ 
EVPAPTTRP EMPPVSCLM DPRLSRDHH HIFSNSDHL 
VGTVEKTLS ILGFEERKP EPITPAKRK LSITEYRQR 
KKLNNNDKT SMDEPVSTE ENNSSESFK PMRPRSDSM 
SSSTSITSS DDEAPAIEM TAPSAFNSE PTELERQRE 
LTTLRLKKV LGIAVDEES RKPALDVEA ILNCELPPV 
VKTIPPSPT FPIPGSCDV TLTAAKSSP ILEAAPPNP 
APVSSSSPP TVENEEPEV ESEEIAEEQ TEVGVKPNM 
FYTPDEEEA GVDENAQGS FEEIDSVDY VPPFNNPVY 
PNDSNFTAF ASVLDEEGR YEGRNPSPP PDLEAGSSP 
YPGQQT
      • >TC016104 RB
        SNDITPPST PTSLAPPTS NNERPPVQE GCVEIVYSG 
PDEDSPSQA IESDAVLEE RVAEMKEEF SNCVVPPLQ 
EVPAPTTRP EMPPVSCLM DPRLSRDHH HIFSNSDHL 
VGTVEKTLS ILGFEERKP EPITPAKRK LSITEYRQR 
KKLNNNDKT SMDEPVSTE ENNSSESFK PMRPRSDSM 
SSSTSITSS DDEAPAIEM TGKAPSAFN SEPTELERQ 
RELTTLRLK KVLGIAVDE ESRKPALDV EAILNCELP 
PVVKTIPPS PTFPIPGSC DVTLTAAKS SPILEAAPP 
NPAPVSSSS PPTVENEEP EVESEEIAE EQTEVGVKP 
NMFYTPDEE EAGVDENAQ GSFEEIDSV DYVPPFNNP 
VYPNDSNFT AFASVLDEE GRYEGRNPS PPPDLEAGS 
SPYPGQQT
      CDS sequence
      • >TC016104 RA
        tcaaatgac attacacca ccatcgaca ccaactagt 
ttagcacct ccaactagt aataatgaa cgtcctccg 
gtccaggaa ggttgtgtc gagatcgtg tacagcggt 
cccgacgaa gacagtccc tcgcaggcc attgaatca 
gatgcagta ttagaagaa cgcgtcgct gaaatgaag 
gaagagttc agtaattgt gtggtgcct ccgttacaa 
gaggtgcct gcgcccaca actcggccc gaaatgccc 
cccgtttca tgtctcatg gatcctcgt ctgtcgagg 
gatcatcat catattttc tcgaatagt gatcattta 
gtcggcact gtggaaaaa acgctgagt attttgggg 
tttgaggag cggaagcct gaaccgatc acaccggcc 
aagcgaaaa ctttcgatt accgaatac aggcaacgg 
aagaagctc aataataat gataaaacg tcaatggac 
gaacctgtc agcactgag gaaaataat tcttcggag 
tcgttcaaa ccgatgcgt ccgaggtcg gatagtatg 
agtagttca acgtcgatc acttcgtcg gatgatgag 
gctccagct atcgaaatg actgctccg tcagcattt 
aattcagag cccaccgag ctcgaacgc caacgtgaa 
ttgaccact ctaaggttg aagaaggtt ttaggcatt 
gcagtggac gaagaaagc cgcaaacca gctttggac 
gtggaagcg attctaaat tgtgaactg ccaccggtc 
gtgaagaca atcccaccg agtccgact ttcccaatt 
ccgggcagt tgtgatgtc acgctaacg gctgcaaag 
tcatcaccg attttggag gctgcgccc ccgaatcct 
gctcctgtg agtagtagt agccctccg actgttgaa 
aatgaagaa ccggaagtt gagagtgaa gaaatcgcc 
gaagagcaa actgaagtt ggtgtcaaa ccgaatatg 
ttttatacg ccggatgag gaagaagct ggagtggat 
gagaacgct caaggcagt tttgaggaa atcgattct 
gttgattat gtgccaccg tttaataat ccggtgtat 
ccgaacgac agcaatttt acagcgttt gcgtcggtt 
ttggatgag gagggccgc tacgagggc cgcaacccc 
tcgccgccc cccgacttg gaggcgggc agctcgccg 
taccccggc caacagacg tga
      • >TC016104 RB
        tcaaatgac attacacca ccatcgaca ccaactagt 
ttagcacct ccaactagt aataatgaa cgtcctccg 
gtccaggaa ggttgtgtc gagatcgtg tacagcggt 
cccgacgaa gacagtccc tcgcaggcc attgaatca 
gatgcagta ttagaagaa cgcgtcgct gaaatgaag 
gaagagttc agtaattgt gtggtgcct ccgttacaa 
gaggtgcct gcgcccaca actcggccc gaaatgccc 
cccgtttca tgtctcatg gatcctcgt ctgtcgagg 
gatcatcat catattttc tcgaatagt gatcattta 
gtcggcact gtggaaaaa acgctgagt attttgggg 
tttgaggag cggaagcct gaaccgatc acaccggcc 
aagcgaaaa ctttcgatt accgaatac aggcaacgg 
aagaagctc aataataat gataaaacg tcaatggac 
gaacctgtc agcactgag gaaaataat tcttcggag 
tcgttcaaa ccgatgcgt ccgaggtcg gatagtatg 
agtagttca acgtcgatc acttcgtcg gatgatgag 
gctccagct atcgaaatg actggtaaa gctccgtca 
gcatttaat tcagagccc accgagctc gaacgccaa 
cgtgaattg accactcta aggttgaag aaggtttta 
ggcattgca gtggacgaa gaaagccgc aaaccagct 
ttggacgtg gaagcgatt ctaaattgt gaactgcca 
ccggtcgtg aagacaatc ccaccgagt ccgactttc 
ccaattccg ggcagttgt gatgtcacg ctaacggct 
gcaaagtca tcaccgatt ttggaggct gcgcccccg 
aatcctgct cctgtgagt agtagtagc cctccgact 
gttgaaaat gaagaaccg gaagttgag agtgaagaa 
atcgccgaa gagcaaact gaagttggt gtcaaaccg 
aatatgttt tatacgccg gatgaggaa gaagctgga 
gtggatgag aacgctcaa ggcagtttt gaggaaatc 
gattctgtt gattatgtg ccaccgttt aataatccg 
gtgtatccg aacgacagc aattttaca gcgtttgcg 
tcggttttg gatgaggag ggccgctac gagggccgc 
aacccctcg ccgcccccc gacttggag gcgggcagc 
tcgccgtac cccggccaa cagacgtga
      mRNA sequence
      • >TC016104 RA
        tctcaaatg acattacac caccatcga caccaacta 
gtttagcac ctccaacta gtaataatg aacgtcctc 
cggtccagg aaggttgtg tcgagatcg tgtacagcg 
gtcccgacg aagacagtc cctcgcagg ccattgaat 
cagatgcag tattagaag aacgcgtcg ctgaaatga 
aggaagagt tcagtaatt gtgtggtgc ctccgttac 
aagaggtgc ctgcgccca caactcggc ccgaaatgc 
cccccgttt catgtctca tggatcctc gtctgtcga 
gggatcatc atcatattt tctcgaata gtgatcatt 
tagtcggca ctgtggaaa aaacgctga gtattttgg 
ggtttgagg agcggaagc ctgaaccga tcacaccgg 
ccaagcgaa aactttcga ttaccgaat acaggcaac 
ggaagaagc tcaataata atgataaaa cgtcaatgg 
acgaacctg tcagcactg aggaaaata attcttcgg 
agtcgttca aaccgatgc gtccgaggt cggatagta 
tgagtagtt caacgtcga tcacttcgt cggatgatg 
aggctccag ctatcgaaa tgactgctc cgtcagcat 
ttaattcag agcccaccg agctcgaac gccaacgtg 
aattgacca ctctaaggt tgaagaagg ttttaggca 
ttgcagtgg acgaagaaa gccgcaaac cagctttgg 
acgtggaag cgattctaa attgtgaac tgccaccgg 
tcgtgaaga caatcccac cgagtccga ctttcccaa 
ttccgggca gttgtgatg tcacgctaa cggctgcaa 
agtcatcac cgattttgg aggctgcgc ccccgaatc 
ctgctcctg tgagtagta gtagccctc cgactgttg 
aaaatgaag aaccggaag ttgagagtg aagaaatcg 
ccgaagagc aaactgaag ttggtgtca aaccgaata 
tgttttata cgccggatg aggaagaag ctggagtgg 
atgagaacg ctcaaggca gttttgagg aaatcgatt 
ctgttgatt atgtgccac cgtttaata atccggtgt 
atccgaacg acagcaatt ttacagcgt ttgcgtcgg 
ttttggatg aggagggcc gctacgagg gccgcaacc 
cctcgccgc cccccgact tggaggcgg gcagctcgc 
cgtaccccg gccaacaga cgtgaactg aaggagctt 
taattagtt taaagaggg tgtgcaatt caagtttct 
tacaacata tttatttaa ttcaagtat taattgtaa 
gcacagtgt gtatatagt tgtttttat ttttggaga 
tttattttt taaaaggat aatatatgt tatcgataa 
atagcgatg cgtaggacc agtgattaa gattttttt 
aagagtttc gttcagtta gcagtttga gttactctt 
actcgtttg tcattggta atatagttt tttttatat 
atttttgta gatgatgat gatgatgag agtatgtac 
actttgtaa ataatatat ataattgaa tttctatcg 
ctaagctgg aattagaaa attgttatt atttttttg 
cgtttaaag tgcagcgtc aatcactct aatcaatac 
tcccgaaaa tctcatttg tacatatta taaattaaa 
tatttgtaa tactcgcga gtttgtggc agtgatttg 
gcgtctggc actgttgta catgtaata aaagtacaa 
aattattta agaggtgat ctcgtgttt taaaaatgt 
agaacttct gtttttatt gattacaaa tagtttttc 
ttagtgtaa aatcgattg ttattacat gttgggtta 
cataggtga attttcaaa taaccatag acagtttct 
agaattgat cgtcaaact gatgataac tttttgtaa 
taatttgtc ttttccatg ttgtttcag tttcgagcg 
gcttccaca aagtgctga agggctcgt aaatatggc 
agtgattta aataagtaa ctgaacaaa atgcatcac 
tggtttatt tgttgaagc cgctctgta catattaca 
tttatcata atctatgtg taaataagt acatttata 
tatgacttt gtaaatttc gtactacaa gcccagttg 
taagttttg tttctccaa tggtacact aaattttta 
tttctattt atttcttct gtatttcag ccatttttg 
caattgatc cagggtcag ttgcattaa agactgtcg 
gaaatttgt ggtatagaa gtctttgca gctgctatt 
tatttcaga taccgaaaa ttgtacaaa atttcgtaa 
taaaatttg ttaaaaaga tataaaaat aaatttctt 
acaatcagt attaattct ataagacgt actgaagta 
agtaacagc gcaaagttg gttaaattt gtcaatgaa 
atgtcaacg actttatta ttacataaa ttaactatt 
ttaatatat aaattaaat aacatggct gtatatata 
actcgatgt tggtacgaa aatcgtacg cattattaa 
ataaattca ttaaaattt a
      • >TC016104 RB
        tctcaaatg acattacac caccatcga caccaacta 
gtttagcac ctccaacta gtaataatg aacgtcctc 
cggtccagg aaggttgtg tcgagatcg tgtacagcg 
gtcccgacg aagacagtc cctcgcagg ccattgaat 
cagatgcag tattagaag aacgcgtcg ctgaaatga 
aggaagagt tcagtaatt gtgtggtgc ctccgttac 
aagaggtgc ctgcgccca caactcggc ccgaaatgc 
cccccgttt catgtctca tggatcctc gtctgtcga 
gggatcatc atcatattt tctcgaata gtgatcatt 
tagtcggca ctgtggaaa aaacgctga gtattttgg 
ggtttgagg agcggaagc ctgaaccga tcacaccgg 
ccaagcgaa aactttcga ttaccgaat acaggcaac 
ggaagaagc tcaataata atgataaaa cgtcaatgg 
acgaacctg tcagcactg aggaaaata attcttcgg 
agtcgttca aaccgatgc gtccgaggt cggatagta 
tgagtagtt caacgtcga tcacttcgt cggatgatg 
aggctccag ctatcgaaa tgactggta aagctccgt 
cagcattta attcagagc ccaccgagc tcgaacgcc 
aacgtgaat tgaccactc taaggttga agaaggttt 
taggcattg cagtggacg aagaaagcc gcaaaccag 
ctttggacg tggaagcga ttctaaatt gtgaactgc 
caccggtcg tgaagacaa tcccaccga gtccgactt 
tcccaattc cgggcagtt gtgatgtca cgctaacgg 
ctgcaaagt catcaccga ttttggagg ctgcgcccc 
cgaatcctg ctcctgtga gtagtagta gccctccga 
ctgttgaaa atgaagaac cggaagttg agagtgaag 
aaatcgccg aagagcaaa ctgaagttg gtgtcaaac 
cgaatatgt tttatacgc cggatgagg aagaagctg 
gagtggatg agaacgctc aaggcagtt ttgaggaaa 
tcgattctg ttgattatg tgccaccgt ttaataatc 
cggtgtatc cgaacgaca gcaatttta cagcgtttg 
cgtcggttt tggatgagg agggccgct acgagggcc 
gcaacccct cgccgcccc ccgacttgg aggcgggca 
gctcgccgt accccggcc aacagacgt gaactgaag 
gagctttaa ttagtttaa agagggtgt gcaattcaa 
gtttcttac aacatattt atttaattc aagtattaa 
ttgtaagca cagtgtgta tatagttgt ttttatttt 
tggagattt attttttaa aaggataat atatgttat 
cgataaata gcgatgcgt aggaccagt gattaagat 
ttttttaag agtttcgtt cagttagca gtttgagtt 
actcttact cgtttgtca ttggtaata tagtttttt 
ttatatatt tttgtagat gatgatgat gatgagagt 
atgtacact ttgtaaata atatatata attgaattt 
ctatcgcta agctggaat tagaaaatt gttattatt 
tttttgcgt ttaaagtgc agcgtcaat cactctaat 
caatactcc cgaaaatct catttgtac atattataa 
attaaatat ttgtaatac tcgcgagtt tgtggcagt 
gatttggcg tctggcact gttgtacat gtaataaaa 
gtacaaaat tatttaaga ggtgatctc gtgttttaa 
aaatgtaga acttctgtt tttattgat tacaaatag 
tttttctta gtgtaaaat cgattgtta ttacatgtt 
gggttacat aggtgaatt ttcaaataa ccatagaca 
gtttctaga attgatcgt caaactgat gataacttt 
ttgtaataa tttgtcttt tccatgttg tttcagttt 
cgagcggct tccacaaag tgctgaagg gctcgtaaa 
tatggcagt gatttaaat aagtaactg aacaaaatg 
catcactgg tttatttgt tgaagccgc tctgtacat 
attacattt atcataatc tatgtgtaa ataagtaca 
tttatatat gactttgta aatttcgta ctacaagcc 
cagttgtaa gttttgttt ctccaatgg tacactaaa 
tttttattt ctatttatt tcttctgta tttcagcca 
tttttgcaa ttgatccag ggtcagttg cattaaaga 
ctgtcggaa atttgtggt atagaagtc tttgcagct 
gctatttat ttcagatac cgaaaattg tacaaaatt 
tcgtaataa aatttgtta aaaagatat aaaaataaa 
tttcttaca atcagtatt aattctata agacgtact 
gaagtaagt aacagcgca aagttggtt aaatttgtc 
aatgaaatg tcaacgact ttattatta cataaatta 
actatttta atatataaa ttaaataac atggctgta 
tatataact cgatgttgg tacgaaaat cgtacgcat 
tattaaata aattcatta aaattta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY