Status
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Gene information
General information
TC016104 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451130.1: 0..4836
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC016104
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05958

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Fertility
      vitellarium shortened - Number of animals showing the phenotype: 4

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva partially ventralnot closed - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection



      adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      Pupal morphology
      wings shortened - Number of animals showing the phenotype: 7

      wings orientation irregular - Number of animals showing the phenotype: 7

      Adult morphology
      flagellomeres number decreased - Number of animals showing the phenotype: 3


      club partially fused - Number of animals showing the phenotype: 3

      wings not closed - Number of animals showing the phenotype: 3


      scutellum flattened - Number of animals showing the phenotype: 3


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016104 RA
        SNDITPPST PTSLAPPTS NNERPPVQE GCVEIVYSG 
        PDEDSPSQA IESDAVLEE RVAEMKEEF SNCVVPPLQ 
        EVPAPTTRP EMPPVSCLM DPRLSRDHH HIFSNSDHL 
        VGTVEKTLS ILGFEERKP EPITPAKRK LSITEYRQR 
        KKLNNNDKT SMDEPVSTE ENNSSESFK PMRPRSDSM 
        SSSTSITSS DDEAPAIEM TAPSAFNSE PTELERQRE 
        LTTLRLKKV LGIAVDEES RKPALDVEA ILNCELPPV 
        VKTIPPSPT FPIPGSCDV TLTAAKSSP ILEAAPPNP 
        APVSSSSPP TVENEEPEV ESEEIAEEQ TEVGVKPNM 
        FYTPDEEEA GVDENAQGS FEEIDSVDY VPPFNNPVY 
        PNDSNFTAF ASVLDEEGR YEGRNPSPP PDLEAGSSP 
        YPGQQT
      • >TC016104 RB
        SNDITPPST PTSLAPPTS NNERPPVQE GCVEIVYSG 
        PDEDSPSQA IESDAVLEE RVAEMKEEF SNCVVPPLQ 
        EVPAPTTRP EMPPVSCLM DPRLSRDHH HIFSNSDHL 
        VGTVEKTLS ILGFEERKP EPITPAKRK LSITEYRQR 
        KKLNNNDKT SMDEPVSTE ENNSSESFK PMRPRSDSM 
        SSSTSITSS DDEAPAIEM TGKAPSAFN SEPTELERQ 
        RELTTLRLK KVLGIAVDE ESRKPALDV EAILNCELP 
        PVVKTIPPS PTFPIPGSC DVTLTAAKS SPILEAAPP 
        NPAPVSSSS PPTVENEEP EVESEEIAE EQTEVGVKP 
        NMFYTPDEE EAGVDENAQ GSFEEIDSV DYVPPFNNP 
        VYPNDSNFT AFASVLDEE GRYEGRNPS PPPDLEAGS 
        SPYPGQQT
      CDS sequence
      • >TC016104 RA
        tcaaatgac attacacca ccatcgaca ccaactagt 
        ttagcacct ccaactagt aataatgaa cgtcctccg 
        gtccaggaa ggttgtgtc gagatcgtg tacagcggt 
        cccgacgaa gacagtccc tcgcaggcc attgaatca 
        gatgcagta ttagaagaa cgcgtcgct gaaatgaag 
        gaagagttc agtaattgt gtggtgcct ccgttacaa 
        gaggtgcct gcgcccaca actcggccc gaaatgccc 
        cccgtttca tgtctcatg gatcctcgt ctgtcgagg 
        gatcatcat catattttc tcgaatagt gatcattta 
        gtcggcact gtggaaaaa acgctgagt attttgggg 
        tttgaggag cggaagcct gaaccgatc acaccggcc 
        aagcgaaaa ctttcgatt accgaatac aggcaacgg 
        aagaagctc aataataat gataaaacg tcaatggac 
        gaacctgtc agcactgag gaaaataat tcttcggag 
        tcgttcaaa ccgatgcgt ccgaggtcg gatagtatg 
        agtagttca acgtcgatc acttcgtcg gatgatgag 
        gctccagct atcgaaatg actgctccg tcagcattt 
        aattcagag cccaccgag ctcgaacgc caacgtgaa 
        ttgaccact ctaaggttg aagaaggtt ttaggcatt 
        gcagtggac gaagaaagc cgcaaacca gctttggac 
        gtggaagcg attctaaat tgtgaactg ccaccggtc 
        gtgaagaca atcccaccg agtccgact ttcccaatt 
        ccgggcagt tgtgatgtc acgctaacg gctgcaaag 
        tcatcaccg attttggag gctgcgccc ccgaatcct 
        gctcctgtg agtagtagt agccctccg actgttgaa 
        aatgaagaa ccggaagtt gagagtgaa gaaatcgcc 
        gaagagcaa actgaagtt ggtgtcaaa ccgaatatg 
        ttttatacg ccggatgag gaagaagct ggagtggat 
        gagaacgct caaggcagt tttgaggaa atcgattct 
        gttgattat gtgccaccg tttaataat ccggtgtat 
        ccgaacgac agcaatttt acagcgttt gcgtcggtt 
        ttggatgag gagggccgc tacgagggc cgcaacccc 
        tcgccgccc cccgacttg gaggcgggc agctcgccg 
        taccccggc caacagacg tga
      • >TC016104 RB
        tcaaatgac attacacca ccatcgaca ccaactagt 
        ttagcacct ccaactagt aataatgaa cgtcctccg 
        gtccaggaa ggttgtgtc gagatcgtg tacagcggt 
        cccgacgaa gacagtccc tcgcaggcc attgaatca 
        gatgcagta ttagaagaa cgcgtcgct gaaatgaag 
        gaagagttc agtaattgt gtggtgcct ccgttacaa 
        gaggtgcct gcgcccaca actcggccc gaaatgccc 
        cccgtttca tgtctcatg gatcctcgt ctgtcgagg 
        gatcatcat catattttc tcgaatagt gatcattta 
        gtcggcact gtggaaaaa acgctgagt attttgggg 
        tttgaggag cggaagcct gaaccgatc acaccggcc 
        aagcgaaaa ctttcgatt accgaatac aggcaacgg 
        aagaagctc aataataat gataaaacg tcaatggac 
        gaacctgtc agcactgag gaaaataat tcttcggag 
        tcgttcaaa ccgatgcgt ccgaggtcg gatagtatg 
        agtagttca acgtcgatc acttcgtcg gatgatgag 
        gctccagct atcgaaatg actggtaaa gctccgtca 
        gcatttaat tcagagccc accgagctc gaacgccaa 
        cgtgaattg accactcta aggttgaag aaggtttta 
        ggcattgca gtggacgaa gaaagccgc aaaccagct 
        ttggacgtg gaagcgatt ctaaattgt gaactgcca 
        ccggtcgtg aagacaatc ccaccgagt ccgactttc 
        ccaattccg ggcagttgt gatgtcacg ctaacggct 
        gcaaagtca tcaccgatt ttggaggct gcgcccccg 
        aatcctgct cctgtgagt agtagtagc cctccgact 
        gttgaaaat gaagaaccg gaagttgag agtgaagaa 
        atcgccgaa gagcaaact gaagttggt gtcaaaccg 
        aatatgttt tatacgccg gatgaggaa gaagctgga 
        gtggatgag aacgctcaa ggcagtttt gaggaaatc 
        gattctgtt gattatgtg ccaccgttt aataatccg 
        gtgtatccg aacgacagc aattttaca gcgtttgcg 
        tcggttttg gatgaggag ggccgctac gagggccgc 
        aacccctcg ccgcccccc gacttggag gcgggcagc 
        tcgccgtac cccggccaa cagacgtga
      mRNA sequence
      • >TC016104 RA
        tctcaaatg acattacac caccatcga caccaacta 
        gtttagcac ctccaacta gtaataatg aacgtcctc 
        cggtccagg aaggttgtg tcgagatcg tgtacagcg 
        gtcccgacg aagacagtc cctcgcagg ccattgaat 
        cagatgcag tattagaag aacgcgtcg ctgaaatga 
        aggaagagt tcagtaatt gtgtggtgc ctccgttac 
        aagaggtgc ctgcgccca caactcggc ccgaaatgc 
        cccccgttt catgtctca tggatcctc gtctgtcga 
        gggatcatc atcatattt tctcgaata gtgatcatt 
        tagtcggca ctgtggaaa aaacgctga gtattttgg 
        ggtttgagg agcggaagc ctgaaccga tcacaccgg 
        ccaagcgaa aactttcga ttaccgaat acaggcaac 
        ggaagaagc tcaataata atgataaaa cgtcaatgg 
        acgaacctg tcagcactg aggaaaata attcttcgg 
        agtcgttca aaccgatgc gtccgaggt cggatagta 
        tgagtagtt caacgtcga tcacttcgt cggatgatg 
        aggctccag ctatcgaaa tgactgctc cgtcagcat 
        ttaattcag agcccaccg agctcgaac gccaacgtg 
        aattgacca ctctaaggt tgaagaagg ttttaggca 
        ttgcagtgg acgaagaaa gccgcaaac cagctttgg 
        acgtggaag cgattctaa attgtgaac tgccaccgg 
        tcgtgaaga caatcccac cgagtccga ctttcccaa 
        ttccgggca gttgtgatg tcacgctaa cggctgcaa 
        agtcatcac cgattttgg aggctgcgc ccccgaatc 
        ctgctcctg tgagtagta gtagccctc cgactgttg 
        aaaatgaag aaccggaag ttgagagtg aagaaatcg 
        ccgaagagc aaactgaag ttggtgtca aaccgaata 
        tgttttata cgccggatg aggaagaag ctggagtgg 
        atgagaacg ctcaaggca gttttgagg aaatcgatt 
        ctgttgatt atgtgccac cgtttaata atccggtgt 
        atccgaacg acagcaatt ttacagcgt ttgcgtcgg 
        ttttggatg aggagggcc gctacgagg gccgcaacc 
        cctcgccgc cccccgact tggaggcgg gcagctcgc 
        cgtaccccg gccaacaga cgtgaactg aaggagctt 
        taattagtt taaagaggg tgtgcaatt caagtttct 
        tacaacata tttatttaa ttcaagtat taattgtaa 
        gcacagtgt gtatatagt tgtttttat ttttggaga 
        tttattttt taaaaggat aatatatgt tatcgataa 
        atagcgatg cgtaggacc agtgattaa gattttttt 
        aagagtttc gttcagtta gcagtttga gttactctt 
        actcgtttg tcattggta atatagttt tttttatat 
        atttttgta gatgatgat gatgatgag agtatgtac 
        actttgtaa ataatatat ataattgaa tttctatcg 
        ctaagctgg aattagaaa attgttatt atttttttg 
        cgtttaaag tgcagcgtc aatcactct aatcaatac 
        tcccgaaaa tctcatttg tacatatta taaattaaa 
        tatttgtaa tactcgcga gtttgtggc agtgatttg 
        gcgtctggc actgttgta catgtaata aaagtacaa 
        aattattta agaggtgat ctcgtgttt taaaaatgt 
        agaacttct gtttttatt gattacaaa tagtttttc 
        ttagtgtaa aatcgattg ttattacat gttgggtta 
        cataggtga attttcaaa taaccatag acagtttct 
        agaattgat cgtcaaact gatgataac tttttgtaa 
        taatttgtc ttttccatg ttgtttcag tttcgagcg 
        gcttccaca aagtgctga agggctcgt aaatatggc 
        agtgattta aataagtaa ctgaacaaa atgcatcac 
        tggtttatt tgttgaagc cgctctgta catattaca 
        tttatcata atctatgtg taaataagt acatttata 
        tatgacttt gtaaatttc gtactacaa gcccagttg 
        taagttttg tttctccaa tggtacact aaattttta 
        tttctattt atttcttct gtatttcag ccatttttg 
        caattgatc cagggtcag ttgcattaa agactgtcg 
        gaaatttgt ggtatagaa gtctttgca gctgctatt 
        tatttcaga taccgaaaa ttgtacaaa atttcgtaa 
        taaaatttg ttaaaaaga tataaaaat aaatttctt 
        acaatcagt attaattct ataagacgt actgaagta 
        agtaacagc gcaaagttg gttaaattt gtcaatgaa 
        atgtcaacg actttatta ttacataaa ttaactatt 
        ttaatatat aaattaaat aacatggct gtatatata 
        actcgatgt tggtacgaa aatcgtacg cattattaa 
        ataaattca ttaaaattt a
      • >TC016104 RB
        tctcaaatg acattacac caccatcga caccaacta 
        gtttagcac ctccaacta gtaataatg aacgtcctc 
        cggtccagg aaggttgtg tcgagatcg tgtacagcg 
        gtcccgacg aagacagtc cctcgcagg ccattgaat 
        cagatgcag tattagaag aacgcgtcg ctgaaatga 
        aggaagagt tcagtaatt gtgtggtgc ctccgttac 
        aagaggtgc ctgcgccca caactcggc ccgaaatgc 
        cccccgttt catgtctca tggatcctc gtctgtcga 
        gggatcatc atcatattt tctcgaata gtgatcatt 
        tagtcggca ctgtggaaa aaacgctga gtattttgg 
        ggtttgagg agcggaagc ctgaaccga tcacaccgg 
        ccaagcgaa aactttcga ttaccgaat acaggcaac 
        ggaagaagc tcaataata atgataaaa cgtcaatgg 
        acgaacctg tcagcactg aggaaaata attcttcgg 
        agtcgttca aaccgatgc gtccgaggt cggatagta 
        tgagtagtt caacgtcga tcacttcgt cggatgatg 
        aggctccag ctatcgaaa tgactggta aagctccgt 
        cagcattta attcagagc ccaccgagc tcgaacgcc 
        aacgtgaat tgaccactc taaggttga agaaggttt 
        taggcattg cagtggacg aagaaagcc gcaaaccag 
        ctttggacg tggaagcga ttctaaatt gtgaactgc 
        caccggtcg tgaagacaa tcccaccga gtccgactt 
        tcccaattc cgggcagtt gtgatgtca cgctaacgg 
        ctgcaaagt catcaccga ttttggagg ctgcgcccc 
        cgaatcctg ctcctgtga gtagtagta gccctccga 
        ctgttgaaa atgaagaac cggaagttg agagtgaag 
        aaatcgccg aagagcaaa ctgaagttg gtgtcaaac 
        cgaatatgt tttatacgc cggatgagg aagaagctg 
        gagtggatg agaacgctc aaggcagtt ttgaggaaa 
        tcgattctg ttgattatg tgccaccgt ttaataatc 
        cggtgtatc cgaacgaca gcaatttta cagcgtttg 
        cgtcggttt tggatgagg agggccgct acgagggcc 
        gcaacccct cgccgcccc ccgacttgg aggcgggca 
        gctcgccgt accccggcc aacagacgt gaactgaag 
        gagctttaa ttagtttaa agagggtgt gcaattcaa 
        gtttcttac aacatattt atttaattc aagtattaa 
        ttgtaagca cagtgtgta tatagttgt ttttatttt 
        tggagattt attttttaa aaggataat atatgttat 
        cgataaata gcgatgcgt aggaccagt gattaagat 
        ttttttaag agtttcgtt cagttagca gtttgagtt 
        actcttact cgtttgtca ttggtaata tagtttttt 
        ttatatatt tttgtagat gatgatgat gatgagagt 
        atgtacact ttgtaaata atatatata attgaattt 
        ctatcgcta agctggaat tagaaaatt gttattatt 
        tttttgcgt ttaaagtgc agcgtcaat cactctaat 
        caatactcc cgaaaatct catttgtac atattataa 
        attaaatat ttgtaatac tcgcgagtt tgtggcagt 
        gatttggcg tctggcact gttgtacat gtaataaaa 
        gtacaaaat tatttaaga ggtgatctc gtgttttaa 
        aaatgtaga acttctgtt tttattgat tacaaatag 
        tttttctta gtgtaaaat cgattgtta ttacatgtt 
        gggttacat aggtgaatt ttcaaataa ccatagaca 
        gtttctaga attgatcgt caaactgat gataacttt 
        ttgtaataa tttgtcttt tccatgttg tttcagttt 
        cgagcggct tccacaaag tgctgaagg gctcgtaaa 
        tatggcagt gatttaaat aagtaactg aacaaaatg 
        catcactgg tttatttgt tgaagccgc tctgtacat 
        attacattt atcataatc tatgtgtaa ataagtaca 
        tttatatat gactttgta aatttcgta ctacaagcc 
        cagttgtaa gttttgttt ctccaatgg tacactaaa 
        tttttattt ctatttatt tcttctgta tttcagcca 
        tttttgcaa ttgatccag ggtcagttg cattaaaga 
        ctgtcggaa atttgtggt atagaagtc tttgcagct 
        gctatttat ttcagatac cgaaaattg tacaaaatt 
        tcgtaataa aatttgtta aaaagatat aaaaataaa 
        tttcttaca atcagtatt aattctata agacgtact 
        gaagtaagt aacagcgca aagttggtt aaatttgtc 
        aatgaaatg tcaacgact ttattatta cataaatta 
        actatttta atatataaa ttaaataac atggctgta 
        tatataact cgatgttgg tacgaaaat cgtacgcat 
        tattaaata aattcatta aaattta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY