General information | |||
TC016104 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451130.1: 0..4836 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC016104 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05958 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva partially ventralnot closed - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
club partially fused - Number of animals showing the phenotype: 3
wings not closed - Number of animals showing the phenotype: 3
scutellum flattened - Number of animals showing the phenotype: 3
- >TC016104 RA
SNDITPPST PTSLAPPTS NNERPPVQE GCVEIVYSG PDEDSPSQA IESDAVLEE RVAEMKEEF SNCVVPPLQ EVPAPTTRP EMPPVSCLM DPRLSRDHH HIFSNSDHL VGTVEKTLS ILGFEERKP EPITPAKRK LSITEYRQR KKLNNNDKT SMDEPVSTE ENNSSESFK PMRPRSDSM SSSTSITSS DDEAPAIEM TAPSAFNSE PTELERQRE LTTLRLKKV LGIAVDEES RKPALDVEA ILNCELPPV VKTIPPSPT FPIPGSCDV TLTAAKSSP ILEAAPPNP APVSSSSPP TVENEEPEV ESEEIAEEQ TEVGVKPNM FYTPDEEEA GVDENAQGS FEEIDSVDY VPPFNNPVY PNDSNFTAF ASVLDEEGR YEGRNPSPP PDLEAGSSP YPGQQT
- >TC016104 RB
SNDITPPST PTSLAPPTS NNERPPVQE GCVEIVYSG PDEDSPSQA IESDAVLEE RVAEMKEEF SNCVVPPLQ EVPAPTTRP EMPPVSCLM DPRLSRDHH HIFSNSDHL VGTVEKTLS ILGFEERKP EPITPAKRK LSITEYRQR KKLNNNDKT SMDEPVSTE ENNSSESFK PMRPRSDSM SSSTSITSS DDEAPAIEM TGKAPSAFN SEPTELERQ RELTTLRLK KVLGIAVDE ESRKPALDV EAILNCELP PVVKTIPPS PTFPIPGSC DVTLTAAKS SPILEAAPP NPAPVSSSS PPTVENEEP EVESEEIAE EQTEVGVKP NMFYTPDEE EAGVDENAQ GSFEEIDSV DYVPPFNNP VYPNDSNFT AFASVLDEE GRYEGRNPS PPPDLEAGS SPYPGQQT
- >TC016104 RA
tcaaatgac attacacca ccatcgaca ccaactagt ttagcacct ccaactagt aataatgaa cgtcctccg gtccaggaa ggttgtgtc gagatcgtg tacagcggt cccgacgaa gacagtccc tcgcaggcc attgaatca gatgcagta ttagaagaa cgcgtcgct gaaatgaag gaagagttc agtaattgt gtggtgcct ccgttacaa gaggtgcct gcgcccaca actcggccc gaaatgccc cccgtttca tgtctcatg gatcctcgt ctgtcgagg gatcatcat catattttc tcgaatagt gatcattta gtcggcact gtggaaaaa acgctgagt attttgggg tttgaggag cggaagcct gaaccgatc acaccggcc aagcgaaaa ctttcgatt accgaatac aggcaacgg aagaagctc aataataat gataaaacg tcaatggac gaacctgtc agcactgag gaaaataat tcttcggag tcgttcaaa ccgatgcgt ccgaggtcg gatagtatg agtagttca acgtcgatc acttcgtcg gatgatgag gctccagct atcgaaatg actgctccg tcagcattt aattcagag cccaccgag ctcgaacgc caacgtgaa ttgaccact ctaaggttg aagaaggtt ttaggcatt gcagtggac gaagaaagc cgcaaacca gctttggac gtggaagcg attctaaat tgtgaactg ccaccggtc gtgaagaca atcccaccg agtccgact ttcccaatt ccgggcagt tgtgatgtc acgctaacg gctgcaaag tcatcaccg attttggag gctgcgccc ccgaatcct gctcctgtg agtagtagt agccctccg actgttgaa aatgaagaa ccggaagtt gagagtgaa gaaatcgcc gaagagcaa actgaagtt ggtgtcaaa ccgaatatg ttttatacg ccggatgag gaagaagct ggagtggat gagaacgct caaggcagt tttgaggaa atcgattct gttgattat gtgccaccg tttaataat ccggtgtat ccgaacgac agcaatttt acagcgttt gcgtcggtt ttggatgag gagggccgc tacgagggc cgcaacccc tcgccgccc cccgacttg gaggcgggc agctcgccg taccccggc caacagacg tga
- >TC016104 RB
tcaaatgac attacacca ccatcgaca ccaactagt ttagcacct ccaactagt aataatgaa cgtcctccg gtccaggaa ggttgtgtc gagatcgtg tacagcggt cccgacgaa gacagtccc tcgcaggcc attgaatca gatgcagta ttagaagaa cgcgtcgct gaaatgaag gaagagttc agtaattgt gtggtgcct ccgttacaa gaggtgcct gcgcccaca actcggccc gaaatgccc cccgtttca tgtctcatg gatcctcgt ctgtcgagg gatcatcat catattttc tcgaatagt gatcattta gtcggcact gtggaaaaa acgctgagt attttgggg tttgaggag cggaagcct gaaccgatc acaccggcc aagcgaaaa ctttcgatt accgaatac aggcaacgg aagaagctc aataataat gataaaacg tcaatggac gaacctgtc agcactgag gaaaataat tcttcggag tcgttcaaa ccgatgcgt ccgaggtcg gatagtatg agtagttca acgtcgatc acttcgtcg gatgatgag gctccagct atcgaaatg actggtaaa gctccgtca gcatttaat tcagagccc accgagctc gaacgccaa cgtgaattg accactcta aggttgaag aaggtttta ggcattgca gtggacgaa gaaagccgc aaaccagct ttggacgtg gaagcgatt ctaaattgt gaactgcca ccggtcgtg aagacaatc ccaccgagt ccgactttc ccaattccg ggcagttgt gatgtcacg ctaacggct gcaaagtca tcaccgatt ttggaggct gcgcccccg aatcctgct cctgtgagt agtagtagc cctccgact gttgaaaat gaagaaccg gaagttgag agtgaagaa atcgccgaa gagcaaact gaagttggt gtcaaaccg aatatgttt tatacgccg gatgaggaa gaagctgga gtggatgag aacgctcaa ggcagtttt gaggaaatc gattctgtt gattatgtg ccaccgttt aataatccg gtgtatccg aacgacagc aattttaca gcgtttgcg tcggttttg gatgaggag ggccgctac gagggccgc aacccctcg ccgcccccc gacttggag gcgggcagc tcgccgtac cccggccaa cagacgtga
- >TC016104 RA
tctcaaatg acattacac caccatcga caccaacta gtttagcac ctccaacta gtaataatg aacgtcctc cggtccagg aaggttgtg tcgagatcg tgtacagcg gtcccgacg aagacagtc cctcgcagg ccattgaat cagatgcag tattagaag aacgcgtcg ctgaaatga aggaagagt tcagtaatt gtgtggtgc ctccgttac aagaggtgc ctgcgccca caactcggc ccgaaatgc cccccgttt catgtctca tggatcctc gtctgtcga gggatcatc atcatattt tctcgaata gtgatcatt tagtcggca ctgtggaaa aaacgctga gtattttgg ggtttgagg agcggaagc ctgaaccga tcacaccgg ccaagcgaa aactttcga ttaccgaat acaggcaac ggaagaagc tcaataata atgataaaa cgtcaatgg acgaacctg tcagcactg aggaaaata attcttcgg agtcgttca aaccgatgc gtccgaggt cggatagta tgagtagtt caacgtcga tcacttcgt cggatgatg aggctccag ctatcgaaa tgactgctc cgtcagcat ttaattcag agcccaccg agctcgaac gccaacgtg aattgacca ctctaaggt tgaagaagg ttttaggca ttgcagtgg acgaagaaa gccgcaaac cagctttgg acgtggaag cgattctaa attgtgaac tgccaccgg tcgtgaaga caatcccac cgagtccga ctttcccaa ttccgggca gttgtgatg tcacgctaa cggctgcaa agtcatcac cgattttgg aggctgcgc ccccgaatc ctgctcctg tgagtagta gtagccctc cgactgttg aaaatgaag aaccggaag ttgagagtg aagaaatcg ccgaagagc aaactgaag ttggtgtca aaccgaata tgttttata cgccggatg aggaagaag ctggagtgg atgagaacg ctcaaggca gttttgagg aaatcgatt ctgttgatt atgtgccac cgtttaata atccggtgt atccgaacg acagcaatt ttacagcgt ttgcgtcgg ttttggatg aggagggcc gctacgagg gccgcaacc cctcgccgc cccccgact tggaggcgg gcagctcgc cgtaccccg gccaacaga cgtgaactg aaggagctt taattagtt taaagaggg tgtgcaatt caagtttct tacaacata tttatttaa ttcaagtat taattgtaa gcacagtgt gtatatagt tgtttttat ttttggaga tttattttt taaaaggat aatatatgt tatcgataa atagcgatg cgtaggacc agtgattaa gattttttt aagagtttc gttcagtta gcagtttga gttactctt actcgtttg tcattggta atatagttt tttttatat atttttgta gatgatgat gatgatgag agtatgtac actttgtaa ataatatat ataattgaa tttctatcg ctaagctgg aattagaaa attgttatt atttttttg cgtttaaag tgcagcgtc aatcactct aatcaatac tcccgaaaa tctcatttg tacatatta taaattaaa tatttgtaa tactcgcga gtttgtggc agtgatttg gcgtctggc actgttgta catgtaata aaagtacaa aattattta agaggtgat ctcgtgttt taaaaatgt agaacttct gtttttatt gattacaaa tagtttttc ttagtgtaa aatcgattg ttattacat gttgggtta cataggtga attttcaaa taaccatag acagtttct agaattgat cgtcaaact gatgataac tttttgtaa taatttgtc ttttccatg ttgtttcag tttcgagcg gcttccaca aagtgctga agggctcgt aaatatggc agtgattta aataagtaa ctgaacaaa atgcatcac tggtttatt tgttgaagc cgctctgta catattaca tttatcata atctatgtg taaataagt acatttata tatgacttt gtaaatttc gtactacaa gcccagttg taagttttg tttctccaa tggtacact aaattttta tttctattt atttcttct gtatttcag ccatttttg caattgatc cagggtcag ttgcattaa agactgtcg gaaatttgt ggtatagaa gtctttgca gctgctatt tatttcaga taccgaaaa ttgtacaaa atttcgtaa taaaatttg ttaaaaaga tataaaaat aaatttctt acaatcagt attaattct ataagacgt actgaagta agtaacagc gcaaagttg gttaaattt gtcaatgaa atgtcaacg actttatta ttacataaa ttaactatt ttaatatat aaattaaat aacatggct gtatatata actcgatgt tggtacgaa aatcgtacg cattattaa ataaattca ttaaaattt a
- >TC016104 RB
tctcaaatg acattacac caccatcga caccaacta gtttagcac ctccaacta gtaataatg aacgtcctc cggtccagg aaggttgtg tcgagatcg tgtacagcg gtcccgacg aagacagtc cctcgcagg ccattgaat cagatgcag tattagaag aacgcgtcg ctgaaatga aggaagagt tcagtaatt gtgtggtgc ctccgttac aagaggtgc ctgcgccca caactcggc ccgaaatgc cccccgttt catgtctca tggatcctc gtctgtcga gggatcatc atcatattt tctcgaata gtgatcatt tagtcggca ctgtggaaa aaacgctga gtattttgg ggtttgagg agcggaagc ctgaaccga tcacaccgg ccaagcgaa aactttcga ttaccgaat acaggcaac ggaagaagc tcaataata atgataaaa cgtcaatgg acgaacctg tcagcactg aggaaaata attcttcgg agtcgttca aaccgatgc gtccgaggt cggatagta tgagtagtt caacgtcga tcacttcgt cggatgatg aggctccag ctatcgaaa tgactggta aagctccgt cagcattta attcagagc ccaccgagc tcgaacgcc aacgtgaat tgaccactc taaggttga agaaggttt taggcattg cagtggacg aagaaagcc gcaaaccag ctttggacg tggaagcga ttctaaatt gtgaactgc caccggtcg tgaagacaa tcccaccga gtccgactt tcccaattc cgggcagtt gtgatgtca cgctaacgg ctgcaaagt catcaccga ttttggagg ctgcgcccc cgaatcctg ctcctgtga gtagtagta gccctccga ctgttgaaa atgaagaac cggaagttg agagtgaag aaatcgccg aagagcaaa ctgaagttg gtgtcaaac cgaatatgt tttatacgc cggatgagg aagaagctg gagtggatg agaacgctc aaggcagtt ttgaggaaa tcgattctg ttgattatg tgccaccgt ttaataatc cggtgtatc cgaacgaca gcaatttta cagcgtttg cgtcggttt tggatgagg agggccgct acgagggcc gcaacccct cgccgcccc ccgacttgg aggcgggca gctcgccgt accccggcc aacagacgt gaactgaag gagctttaa ttagtttaa agagggtgt gcaattcaa gtttcttac aacatattt atttaattc aagtattaa ttgtaagca cagtgtgta tatagttgt ttttatttt tggagattt attttttaa aaggataat atatgttat cgataaata gcgatgcgt aggaccagt gattaagat ttttttaag agtttcgtt cagttagca gtttgagtt actcttact cgtttgtca ttggtaata tagtttttt ttatatatt tttgtagat gatgatgat gatgagagt atgtacact ttgtaaata atatatata attgaattt ctatcgcta agctggaat tagaaaatt gttattatt tttttgcgt ttaaagtgc agcgtcaat cactctaat caatactcc cgaaaatct catttgtac atattataa attaaatat ttgtaatac tcgcgagtt tgtggcagt gatttggcg tctggcact gttgtacat gtaataaaa gtacaaaat tatttaaga ggtgatctc gtgttttaa aaatgtaga acttctgtt tttattgat tacaaatag tttttctta gtgtaaaat cgattgtta ttacatgtt gggttacat aggtgaatt ttcaaataa ccatagaca gtttctaga attgatcgt caaactgat gataacttt ttgtaataa tttgtcttt tccatgttg tttcagttt cgagcggct tccacaaag tgctgaagg gctcgtaaa tatggcagt gatttaaat aagtaactg aacaaaatg catcactgg tttatttgt tgaagccgc tctgtacat attacattt atcataatc tatgtgtaa ataagtaca tttatatat gactttgta aatttcgta ctacaagcc cagttgtaa gttttgttt ctccaatgg tacactaaa tttttattt ctatttatt tcttctgta tttcagcca tttttgcaa ttgatccag ggtcagttg cattaaaga ctgtcggaa atttgtggt atagaagtc tttgcagct gctatttat ttcagatac cgaaaattg tacaaaatt tcgtaataa aatttgtta aaaagatat aaaaataaa tttcttaca atcagtatt aattctata agacgtact gaagtaagt aacagcgca aagttggtt aaatttgtc aatgaaatg tcaacgact ttattatta cataaatta actatttta atatataaa ttaaataac atggctgta tatataact cgatgttgg tacgaaaat cgtacgcat tattaaata aattcatta aaattta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
