| General information | |||
| TC016087 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 1316316..1341873 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC016087 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05957 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC016087 RA
MADLIRAGS GHGAVMKDK LGSPSTGSE DGNEQDTQG APKAEPDET DLGDSAPFA PSETLWYHG RLDRYQAEE RLWISNKLG SYLVRESDR KPGSYVLSF LGRTGMNHF RITAVCGDF YIGGRQFVS LNDLVGYYT ERSDLLKRE RLVHPVPPP EPVNDKKRV VAILPYTKM PDTDELTFK KGDIFFVHN DMGNGWLWV TAHRTGEQG MIFRELVED LDDSIDPNT VFPWFHPNC TKNEAVDLL VKAGPGSFL VRPSDNSPG DYSLFFHIN NQIQRFRIE KKGVRYLMG GRIFECLDA VINRYRKEQ IVEGYTLQH PLVVDGSKQ VDWVPDNPK VKSEAEKIY ATLRECRDQ AGLKKMRGI KHQGFLHRK SDKAAKKWK LLYFALVVD GTDTHLYLY ENPKRTKPK GLIDLSCAY LYQVHDSLW ERPHCLQIV ERALPCLAT VTHLAAPST EECQGWVNA LKPLCVPQL SRATCKVPR LRELRCLTL HILDAHRLP YKLVPQPYV TIQFNNQVR IARTRAKSG PDPVWDEEF VFDDVPCDV VSFTLTVHN KGKRGKDTE VAELHVDLA TLGNGEEKE EWYTLSGLT PMGEWGSLR LRTRYLHDL VMPAEEYSP LQQLLLQGG LASVKTLAE ISHADRAPL ATALLRVFR AEGRETELL IELNSAEIE RETETSTLF RAASLSTTL MDLYMRAEC RGFLHAAVL ETVLRLLDS KQSAELNPA KMDSLDDAC SNAEFLLQV LDQVALSIF MSAEACPRS VRYICGCLQ KAVGAKWPD ERHVRARVV SGFIFLRLL CPALLNPRQ FGLVNEQPS PSATRSLVM VAKCLQNLA NLVEFGGKE PYMEVVNPF ILKNKERMV VFLDHLSNV PDPGEPRLT SKPDTAKEL ATLHHICVA HLTELQAVA KVNSNIKTL VTVTEMLAT HKQKYLEMI R
- >TC016087 RA
atggccgat ttgatacgt gcaggaagc ggacatggg gctgtgatg aaagataag ctgggttcg ccgagcacc ggaagcgag gatggcaac gagcaggac acgcagggg gcccccaag gcagagccc gacgagacc gacctgggg gactcggcg ccgttcgcc ccctcggag acgctgtgg taccacgga cggctggac cggtaccag gccgaggag cgcctctgg atttcgaac aaattgggg agctatctg gtgcgggag agtgaccgg aagccgggc tcgtatgtg ttgtcgttt ttggggcgc acgggaatg aaccatttt cggattacg gccgtttgt ggcgatttt tacattggg ggaagacag tttgtgtct ttgaatgat ctggttggg tattatacg gagaggagt gatttgttg aagagggaa cggttggtg catccggtg ccacctccg gagccagtc aatgataaa aagagggtg gtggcaatt ttgccatac acgaaaatg ccggacacc gacgaacta acattcaaa aaaggcgac attttcttt gtccacaac gacatgggc aatgggtgg ttatgggtc acggcccat cgaacgggc gaacaaggg atgattttc cgggaattg gtggaagat ctggatgat agcattgat cccaatacg gtttttcca tggtttcat ccaaattgt acgaaaaac gaagcggtg gatttactc gtcaaagcc ggccctgga agtttcctc gtgcgtccc agcgacaat tctcccggc gattattcg ttatttttc cacataaat aatcaaatc caacgattt cgaatcgag aaaaaagga gtgcgttat cttatgggg ggccgtatt ttcgaatgt ctcgatgct gttatcaac cgttatcgc aaggagcaa atcgttgaa ggatatact ttacaacat cccttggtc gttgacgga agcaaacaa gtcgattgg gtgccagat aatccaaaa gttaaatcc gaagctgag aaaatctac gccactttg cgtgaatgt cgcgatcaa gcgggcttg aaaaaaatg cgagggatc aaacatcag ggttttcta catcgaaaa tcggacaaa gctgccaaa aaatggaaa cttttgtac tttgcgttg gttgtcgat gggaccgat acgcatctg tatttgtac gaaaatccc aaaaggacg aagccgaag gggctcatc gacctgtcc tgtgcgtat ctctaccag gtccatgat agcctctgg gagcgcccc cattgctta caaattgtg gaacgcgct cttccctgt ctagcgacg gtcacccat ctcgcagcg ccctctacc gaggagtgc caaggctgg gtgaacgcc ttaaagcct ctctgtgtt ccccaatta agtcgggcc acatgtaaa gtcccacga cttcgtgaa ttacgatgt ttgacttta cacattttg gacgctcac aggctgcca tataaacta gtgccacaa ccatacgtt accatacaa ttcaataat caagtgaga atcgctcgg acgagggcc aagtccggg cctgaccca gtatgggac gaggagttt gtttttgat gatgtgcca tgtgatgta gtttcgttt actttgact gtacataat aaagggaag cgggggaag gacacggaa gtggctgaa cttcatgta gatttggcc acgttaggg aacggcgag gagaaggag gaatggtat actttatca gggttgacg ccaatgggg gaatggggg tcgcttagg ttaaggaca agatattta cacgacttg gtgatgccg gctgaagaa tacagcccc ttgcagcag ttactccta caagggggc ctggcgtcg gtcaagacc cttgctgag atatctcac gcagatagg gcgcctcta gctacagcc cttttaagg gtttttagg gcagagggt cgtgagact gaacttttg atcgagttg aattcggct gaaatcgaa cgtgaaact gaaacatcg acgctattc cgtgccgct tcgctcagt actactttg atggacttg tatatgagg gcggaatgt agaggtttt ctgcacgct gctgttctc gaaaccgtt ttgaggctg ttggatagt aagcaaagt gcggaatta aatccggcg aaaatggat tcgttagat gatgcatgt agtaatgcg gagttcttg ttgcaagtg ttggatcag gtcgccttg agcatattc atgtcggcg gaggcatgc ccccgttca gtccgctac atttgcggt tgcctccaa aaggccgtg ggggcaaaa tggcccgat gagcgccac gtgcgcgct cgagtcgtt tccggcttc atttttcta cgtctttta tgtcccgca ttgctcaat ccgagacaa ttcggtctt gtaaacgaa caaccgtca ccatcggcc acccgaagc ctcgtcatg gtcgccaaa tgtctgcaa aatctcgca aatttggtc gaattcggc ggaaaagag ccgtacatg gaggtggtg aacccgttc attttgaag aataaagag cgcatggtg gtgttcctg gaccacctc tccaacgtg ccagatcct ggtgagccc cgtcttacg agcaaacct gatactgcc aaagaatta gcaactttg catcatatc tgcgttgca catcttacc gaattacaa gcggtggcg aaagtcaat tccaatatt aagactttg gtcactgtt accgaaatg ttggcaaca cataaacaa aagtatctc gagatgata agatag
- >TC016087 RA
gttgtgtcc ccacttggc agcgccgcc ttcctgagt cactcatcg gaatttgtc aaaataatt tcgtgtgtt cgttcccct cacagaatc aaataaacg gttcgcgag acatcaata aaccattca tgagatcgg ccacaggtt aggtggtgc agcgcgtca tttccgagc gtggttatg gtttaaagt gtcgaaatg gccgatttg atacgtgca ggaagcgga catggggct gtgatgaaa gataagctg ggttcgccg agcaccgga agcgaggat ggcaacgag caggacacg cagggggcc cccaaggca gagcccgac gagaccgac ctgggggac tcggcgccg ttcgccccc tcggagacg ctgtggtac cacggacgg ctggaccgg taccaggcc gaggagcgc ctctggatt tcgaacaaa ttggggagc tatctggtg cgggagagt gaccggaag ccgggctcg tatgtgttg tcgtttttg gggcgcacg ggaatgaac cattttcgg attacggcc gtttgtggc gatttttac attggggga agacagttt gtgtctttg aatgatctg gttgggtat tatacggag aggagtgat ttgttgaag agggaacgg ttggtgcat ccggtgcca cctccggag ccagtcaat gataaaaag agggtggtg gcaattttg ccatacacg aaaatgccg gacaccgac gaactaaca ttcaaaaaa ggcgacatt ttctttgtc cacaacgac atgggcaat gggtggtta tgggtcacg gcccatcga acgggcgaa caagggatg attttccgg gaattggtg gaagatctg gatgatagc attgatccc aatacggtt tttccatgg tttcatcca aattgtacg aaaaacgaa gcggtggat ttactcgtc aaagccggc cctggaagt ttcctcgtg cgtcccagc gacaattct cccggcgat tattcgtta tttttccac ataaataat caaatccaa cgatttcga atcgagaaa aaaggagtg cgttatctt atggggggc cgtattttc gaatgtctc gatgctgtt atcaaccgt tatcgcaag gagcaaatc gttgaagga tatacttta caacatccc ttggtcgtt gacggaagc aaacaagtc gattgggtg ccagataat ccaaaagtt aaatccgaa gctgagaaa atctacgcc actttgcgt gaatgtcgc gatcaagcg ggcttgaaa aaaatgcga gggatcaaa catcagggt tttctacat cgaaaatcg gacaaagct gccaaaaaa tggaaactt ttgtacttt gcgttggtt gtcgatggg accgatacg catctgtat ttgtacgaa aatcccaaa aggacgaag ccgaagggg ctcatcgac ctgtcctgt gcgtatctc taccaggtc catgatagc ctctgggag cgcccccat tgcttacaa attgtggaa cgcgctctt ccctgtcta gcgacggtc acccatctc gcagcgccc tctaccgag gagtgccaa ggctgggtg aacgcctta aagcctctc tgtgttccc caattaagt cgggccaca tgtaaagtc ccacgactt cgtgaatta cgatgtttg actttacac attttggac gctcacagg ctgccatat aaactagtg ccacaacca tacgttacc atacaattc aataatcaa gtgagaatc gctcggacg agggccaag tccgggcct gacccagta tgggacgag gagtttgtt tttgatgat gtgccatgt gatgtagtt tcgtttact ttgactgta cataataaa gggaagcgg gggaaggac acggaagtg gctgaactt catgtagat ttggccacg ttagggaac ggcgaggag aaggaggaa tggtatact ttatcaggg ttgacgcca atgggggaa tgggggtcg cttaggtta aggacaaga tatttacac gacttggtg atgccggct gaagaatac agccccttg cagcagtta ctcctacaa gggggcctg gcgtcggtc aagaccctt gctgagata tctcacgca gatagggcg cctctagct acagccctt ttaagggtt tttagggca gagggtcgt gagactgaa cttttgatc gagttgaat tcggctgaa atcgaacgt gaaactgaa acatcgacg ctattccgt gccgcttcg ctcagtact actttgatg gacttgtat atgagggcg gaatgtaga ggttttctg cacgctgct gttctcgaa accgttttg aggctgttg gatagtaag caaagtgcg gaattaaat ccggcgaaa atggattcg ttagatgat gcatgtagt aatgcggag ttcttgttg caagtgttg gatcaggtc gccttgagc atattcatg tcggcggag gcatgcccc cgttcagtc cgctacatt tgcggttgc ctccaaaag gccgtgggg gcaaaatgg cccgatgag cgccacgtg cgcgctcga gtcgtttcc ggcttcatt tttctacgt cttttatgt cccgcattg ctcaatccg agacaattc ggtcttgta aacgaacaa ccgtcacca tcggccacc cgaagcctc gtcatggtc gccaaatgt ctgcaaaat ctcgcaaat ttggtcgaa ttcggcgga aaagagccg tacatggag gtggtgaac ccgttcatt ttgaagaat aaagagcgc atggtggtg ttcctggac cacctctcc aacgtgcca gatcctggt gagccccgt cttacgagc aaacctgat actgccaaa gaattagca actttgcat catatctgc gttgcacat cttaccgaa ttacaagcg gtggcgaaa gtcaattcc aatattaag actttggtc actgttacc gaaatgttg gcaacacat aaacaaaag tatctcgag atgataaga tagtagttt ggttgagtt cggggattt actgggggt ttttcaaaa tttttatta ttatttttt tgtacttaa atcatcgct ctcggggat tttggagtg cgtttagtt gttgatcct tacgtaagg aaacgaaca ccactgatt atgtttgta ggatgtgta cactaggtg gttagcatg tgatgtgct ctcttagta atcaaaatc tagactgta ttatttttt attacaaga ctgtcatta aaaaaagtt aattctaag tcagtaggt tgcctaaca tggaacaga tagttatta atcgatttc agtgtcaaa ctctatcca atattttta tttatttat cgtacgcac actcatttg agtgtgtta accacagct tccttctct gaaaattgc agagttggg tcacttttt tattaaaat gtttgtgag caaattaat taggttcat atctaattt aaccagcga cgtattttg agtgccatc taaaaaaaa aaaatagtt gaatacagg taactctga tcaaaagaa gttgggtat atcagtaca ttttaaaga atatcagca gaagcttga aaactaatc tcatatgtg ttttctatg aaaattttt attttatcg tttagagtt gtactggtt ttatatctt ccagtaaaa aaaaaactt attttttgt taacatttt tagttaaaa acattgagt ctcaattaa atacactgc taatatcac tctaactaa ttggacatt taaaaacgt tggttaact tactgcaat ccaaaataa aattttgta tcgtcatta aatacaact tagcttggt accaaaaat ctacagaaa aaaatggag ttaatagag gaagaaaga aaataaaaa taaagtgtt agaacagta ccatctttc aaaaaaaat gcgagatat tgaggaata cgaaaaaat aaagctttt ggaattgga aataaaaat taaagaaga aa
