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Gene information
General information
TC016087 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1316316..1341873
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016087
    iBeetle screen iB_05957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016087 RA
      MADLIRAGS GHGAVMKDK LGSPSTGSE DGNEQDTQG 
      APKAEPDET DLGDSAPFA PSETLWYHG RLDRYQAEE 
      RLWISNKLG SYLVRESDR KPGSYVLSF LGRTGMNHF 
      RITAVCGDF YIGGRQFVS LNDLVGYYT ERSDLLKRE 
      RLVHPVPPP EPVNDKKRV VAILPYTKM PDTDELTFK 
      KGDIFFVHN DMGNGWLWV TAHRTGEQG MIFRELVED 
      LDDSIDPNT VFPWFHPNC TKNEAVDLL VKAGPGSFL 
      VRPSDNSPG DYSLFFHIN NQIQRFRIE KKGVRYLMG 
      GRIFECLDA VINRYRKEQ IVEGYTLQH PLVVDGSKQ 
      VDWVPDNPK VKSEAEKIY ATLRECRDQ AGLKKMRGI 
      KHQGFLHRK SDKAAKKWK LLYFALVVD GTDTHLYLY 
      ENPKRTKPK GLIDLSCAY LYQVHDSLW ERPHCLQIV 
      ERALPCLAT VTHLAAPST EECQGWVNA LKPLCVPQL 
      SRATCKVPR LRELRCLTL HILDAHRLP YKLVPQPYV 
      TIQFNNQVR IARTRAKSG PDPVWDEEF VFDDVPCDV 
      VSFTLTVHN KGKRGKDTE VAELHVDLA TLGNGEEKE 
      EWYTLSGLT PMGEWGSLR LRTRYLHDL VMPAEEYSP 
      LQQLLLQGG LASVKTLAE ISHADRAPL ATALLRVFR 
      AEGRETELL IELNSAEIE RETETSTLF RAASLSTTL 
      MDLYMRAEC RGFLHAAVL ETVLRLLDS KQSAELNPA 
      KMDSLDDAC SNAEFLLQV LDQVALSIF MSAEACPRS 
      VRYICGCLQ KAVGAKWPD ERHVRARVV SGFIFLRLL 
      CPALLNPRQ FGLVNEQPS PSATRSLVM VAKCLQNLA 
      NLVEFGGKE PYMEVVNPF ILKNKERMV VFLDHLSNV 
      PDPGEPRLT SKPDTAKEL ATLHHICVA HLTELQAVA 
      KVNSNIKTL VTVTEMLAT HKQKYLEMI R
    CDS sequence
    • >TC016087 RA
      atggccgat ttgatacgt gcaggaagc ggacatggg 
      gctgtgatg aaagataag ctgggttcg ccgagcacc 
      ggaagcgag gatggcaac gagcaggac acgcagggg 
      gcccccaag gcagagccc gacgagacc gacctgggg 
      gactcggcg ccgttcgcc ccctcggag acgctgtgg 
      taccacgga cggctggac cggtaccag gccgaggag 
      cgcctctgg atttcgaac aaattgggg agctatctg 
      gtgcgggag agtgaccgg aagccgggc tcgtatgtg 
      ttgtcgttt ttggggcgc acgggaatg aaccatttt 
      cggattacg gccgtttgt ggcgatttt tacattggg 
      ggaagacag tttgtgtct ttgaatgat ctggttggg 
      tattatacg gagaggagt gatttgttg aagagggaa 
      cggttggtg catccggtg ccacctccg gagccagtc 
      aatgataaa aagagggtg gtggcaatt ttgccatac 
      acgaaaatg ccggacacc gacgaacta acattcaaa 
      aaaggcgac attttcttt gtccacaac gacatgggc 
      aatgggtgg ttatgggtc acggcccat cgaacgggc 
      gaacaaggg atgattttc cgggaattg gtggaagat 
      ctggatgat agcattgat cccaatacg gtttttcca 
      tggtttcat ccaaattgt acgaaaaac gaagcggtg 
      gatttactc gtcaaagcc ggccctgga agtttcctc 
      gtgcgtccc agcgacaat tctcccggc gattattcg 
      ttatttttc cacataaat aatcaaatc caacgattt 
      cgaatcgag aaaaaagga gtgcgttat cttatgggg 
      ggccgtatt ttcgaatgt ctcgatgct gttatcaac 
      cgttatcgc aaggagcaa atcgttgaa ggatatact 
      ttacaacat cccttggtc gttgacgga agcaaacaa 
      gtcgattgg gtgccagat aatccaaaa gttaaatcc 
      gaagctgag aaaatctac gccactttg cgtgaatgt 
      cgcgatcaa gcgggcttg aaaaaaatg cgagggatc 
      aaacatcag ggttttcta catcgaaaa tcggacaaa 
      gctgccaaa aaatggaaa cttttgtac tttgcgttg 
      gttgtcgat gggaccgat acgcatctg tatttgtac 
      gaaaatccc aaaaggacg aagccgaag gggctcatc 
      gacctgtcc tgtgcgtat ctctaccag gtccatgat 
      agcctctgg gagcgcccc cattgctta caaattgtg 
      gaacgcgct cttccctgt ctagcgacg gtcacccat 
      ctcgcagcg ccctctacc gaggagtgc caaggctgg 
      gtgaacgcc ttaaagcct ctctgtgtt ccccaatta 
      agtcgggcc acatgtaaa gtcccacga cttcgtgaa 
      ttacgatgt ttgacttta cacattttg gacgctcac 
      aggctgcca tataaacta gtgccacaa ccatacgtt 
      accatacaa ttcaataat caagtgaga atcgctcgg 
      acgagggcc aagtccggg cctgaccca gtatgggac 
      gaggagttt gtttttgat gatgtgcca tgtgatgta 
      gtttcgttt actttgact gtacataat aaagggaag 
      cgggggaag gacacggaa gtggctgaa cttcatgta 
      gatttggcc acgttaggg aacggcgag gagaaggag 
      gaatggtat actttatca gggttgacg ccaatgggg 
      gaatggggg tcgcttagg ttaaggaca agatattta 
      cacgacttg gtgatgccg gctgaagaa tacagcccc 
      ttgcagcag ttactccta caagggggc ctggcgtcg 
      gtcaagacc cttgctgag atatctcac gcagatagg 
      gcgcctcta gctacagcc cttttaagg gtttttagg 
      gcagagggt cgtgagact gaacttttg atcgagttg 
      aattcggct gaaatcgaa cgtgaaact gaaacatcg 
      acgctattc cgtgccgct tcgctcagt actactttg 
      atggacttg tatatgagg gcggaatgt agaggtttt 
      ctgcacgct gctgttctc gaaaccgtt ttgaggctg 
      ttggatagt aagcaaagt gcggaatta aatccggcg 
      aaaatggat tcgttagat gatgcatgt agtaatgcg 
      gagttcttg ttgcaagtg ttggatcag gtcgccttg 
      agcatattc atgtcggcg gaggcatgc ccccgttca 
      gtccgctac atttgcggt tgcctccaa aaggccgtg 
      ggggcaaaa tggcccgat gagcgccac gtgcgcgct 
      cgagtcgtt tccggcttc atttttcta cgtctttta 
      tgtcccgca ttgctcaat ccgagacaa ttcggtctt 
      gtaaacgaa caaccgtca ccatcggcc acccgaagc 
      ctcgtcatg gtcgccaaa tgtctgcaa aatctcgca 
      aatttggtc gaattcggc ggaaaagag ccgtacatg 
      gaggtggtg aacccgttc attttgaag aataaagag 
      cgcatggtg gtgttcctg gaccacctc tccaacgtg 
      ccagatcct ggtgagccc cgtcttacg agcaaacct 
      gatactgcc aaagaatta gcaactttg catcatatc 
      tgcgttgca catcttacc gaattacaa gcggtggcg 
      aaagtcaat tccaatatt aagactttg gtcactgtt 
      accgaaatg ttggcaaca cataaacaa aagtatctc 
      gagatgata agatag
    mRNA sequence
    • >TC016087 RA
      gttgtgtcc ccacttggc agcgccgcc ttcctgagt 
      cactcatcg gaatttgtc aaaataatt tcgtgtgtt 
      cgttcccct cacagaatc aaataaacg gttcgcgag 
      acatcaata aaccattca tgagatcgg ccacaggtt 
      aggtggtgc agcgcgtca tttccgagc gtggttatg 
      gtttaaagt gtcgaaatg gccgatttg atacgtgca 
      ggaagcgga catggggct gtgatgaaa gataagctg 
      ggttcgccg agcaccgga agcgaggat ggcaacgag 
      caggacacg cagggggcc cccaaggca gagcccgac 
      gagaccgac ctgggggac tcggcgccg ttcgccccc 
      tcggagacg ctgtggtac cacggacgg ctggaccgg 
      taccaggcc gaggagcgc ctctggatt tcgaacaaa 
      ttggggagc tatctggtg cgggagagt gaccggaag 
      ccgggctcg tatgtgttg tcgtttttg gggcgcacg 
      ggaatgaac cattttcgg attacggcc gtttgtggc 
      gatttttac attggggga agacagttt gtgtctttg 
      aatgatctg gttgggtat tatacggag aggagtgat 
      ttgttgaag agggaacgg ttggtgcat ccggtgcca 
      cctccggag ccagtcaat gataaaaag agggtggtg 
      gcaattttg ccatacacg aaaatgccg gacaccgac 
      gaactaaca ttcaaaaaa ggcgacatt ttctttgtc 
      cacaacgac atgggcaat gggtggtta tgggtcacg 
      gcccatcga acgggcgaa caagggatg attttccgg 
      gaattggtg gaagatctg gatgatagc attgatccc 
      aatacggtt tttccatgg tttcatcca aattgtacg 
      aaaaacgaa gcggtggat ttactcgtc aaagccggc 
      cctggaagt ttcctcgtg cgtcccagc gacaattct 
      cccggcgat tattcgtta tttttccac ataaataat 
      caaatccaa cgatttcga atcgagaaa aaaggagtg 
      cgttatctt atggggggc cgtattttc gaatgtctc 
      gatgctgtt atcaaccgt tatcgcaag gagcaaatc 
      gttgaagga tatacttta caacatccc ttggtcgtt 
      gacggaagc aaacaagtc gattgggtg ccagataat 
      ccaaaagtt aaatccgaa gctgagaaa atctacgcc 
      actttgcgt gaatgtcgc gatcaagcg ggcttgaaa 
      aaaatgcga gggatcaaa catcagggt tttctacat 
      cgaaaatcg gacaaagct gccaaaaaa tggaaactt 
      ttgtacttt gcgttggtt gtcgatggg accgatacg 
      catctgtat ttgtacgaa aatcccaaa aggacgaag 
      ccgaagggg ctcatcgac ctgtcctgt gcgtatctc 
      taccaggtc catgatagc ctctgggag cgcccccat 
      tgcttacaa attgtggaa cgcgctctt ccctgtcta 
      gcgacggtc acccatctc gcagcgccc tctaccgag 
      gagtgccaa ggctgggtg aacgcctta aagcctctc 
      tgtgttccc caattaagt cgggccaca tgtaaagtc 
      ccacgactt cgtgaatta cgatgtttg actttacac 
      attttggac gctcacagg ctgccatat aaactagtg 
      ccacaacca tacgttacc atacaattc aataatcaa 
      gtgagaatc gctcggacg agggccaag tccgggcct 
      gacccagta tgggacgag gagtttgtt tttgatgat 
      gtgccatgt gatgtagtt tcgtttact ttgactgta 
      cataataaa gggaagcgg gggaaggac acggaagtg 
      gctgaactt catgtagat ttggccacg ttagggaac 
      ggcgaggag aaggaggaa tggtatact ttatcaggg 
      ttgacgcca atgggggaa tgggggtcg cttaggtta 
      aggacaaga tatttacac gacttggtg atgccggct 
      gaagaatac agccccttg cagcagtta ctcctacaa 
      gggggcctg gcgtcggtc aagaccctt gctgagata 
      tctcacgca gatagggcg cctctagct acagccctt 
      ttaagggtt tttagggca gagggtcgt gagactgaa 
      cttttgatc gagttgaat tcggctgaa atcgaacgt 
      gaaactgaa acatcgacg ctattccgt gccgcttcg 
      ctcagtact actttgatg gacttgtat atgagggcg 
      gaatgtaga ggttttctg cacgctgct gttctcgaa 
      accgttttg aggctgttg gatagtaag caaagtgcg 
      gaattaaat ccggcgaaa atggattcg ttagatgat 
      gcatgtagt aatgcggag ttcttgttg caagtgttg 
      gatcaggtc gccttgagc atattcatg tcggcggag 
      gcatgcccc cgttcagtc cgctacatt tgcggttgc 
      ctccaaaag gccgtgggg gcaaaatgg cccgatgag 
      cgccacgtg cgcgctcga gtcgtttcc ggcttcatt 
      tttctacgt cttttatgt cccgcattg ctcaatccg 
      agacaattc ggtcttgta aacgaacaa ccgtcacca 
      tcggccacc cgaagcctc gtcatggtc gccaaatgt 
      ctgcaaaat ctcgcaaat ttggtcgaa ttcggcgga 
      aaagagccg tacatggag gtggtgaac ccgttcatt 
      ttgaagaat aaagagcgc atggtggtg ttcctggac 
      cacctctcc aacgtgcca gatcctggt gagccccgt 
      cttacgagc aaacctgat actgccaaa gaattagca 
      actttgcat catatctgc gttgcacat cttaccgaa 
      ttacaagcg gtggcgaaa gtcaattcc aatattaag 
      actttggtc actgttacc gaaatgttg gcaacacat 
      aaacaaaag tatctcgag atgataaga tagtagttt 
      ggttgagtt cggggattt actgggggt ttttcaaaa 
      tttttatta ttatttttt tgtacttaa atcatcgct 
      ctcggggat tttggagtg cgtttagtt gttgatcct 
      tacgtaagg aaacgaaca ccactgatt atgtttgta 
      ggatgtgta cactaggtg gttagcatg tgatgtgct 
      ctcttagta atcaaaatc tagactgta ttatttttt 
      attacaaga ctgtcatta aaaaaagtt aattctaag 
      tcagtaggt tgcctaaca tggaacaga tagttatta 
      atcgatttc agtgtcaaa ctctatcca atattttta 
      tttatttat cgtacgcac actcatttg agtgtgtta 
      accacagct tccttctct gaaaattgc agagttggg 
      tcacttttt tattaaaat gtttgtgag caaattaat 
      taggttcat atctaattt aaccagcga cgtattttg 
      agtgccatc taaaaaaaa aaaatagtt gaatacagg 
      taactctga tcaaaagaa gttgggtat atcagtaca 
      ttttaaaga atatcagca gaagcttga aaactaatc 
      tcatatgtg ttttctatg aaaattttt attttatcg 
      tttagagtt gtactggtt ttatatctt ccagtaaaa 
      aaaaaactt attttttgt taacatttt tagttaaaa 
      acattgagt ctcaattaa atacactgc taatatcac 
      tctaactaa ttggacatt taaaaacgt tggttaact 
      tactgcaat ccaaaataa aattttgta tcgtcatta 
      aatacaact tagcttggt accaaaaat ctacagaaa 
      aaaatggag ttaatagag gaagaaaga aaataaaaa 
      taaagtgtt agaacagta ccatctttc aaaaaaaat 
      gcgagatat tgaggaata cgaaaaaat aaagctttt 
      ggaattgga aataaaaat taaagaaga aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY