Status
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Gene information
General information
TC016058 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2127952..2131842
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016058
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05951

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016058 RA
      MFVALLCSL LATQQIAAV VNNSKSASI EESSNKTAS 
      AAKIPDSVS SASEQEAKR RDAPLSDTY GAPLSGGGI 
      DSYVPSGPS NPGLPIPVY GVPDAPSNN IVYPAPPPD 
      IPPPLPQIS SSYGVPFKT YGPPQLINN FDLGHSTDF 
      VPPPPPTKY LPPIRSHKP FSAPKLPRP RPHYGPPQK 
      PIFVPKLPK PNYGPPKSV YGPPKQPLF VPQKTNFKQ 
      QSVTIVTVP SNNYGVPNL GHGQDAFIS PPAAIGHGH 
      YGPPDPVPH APPPGVPAP PTPPDIKYD GWQPIPGLV 
      SRPPDTYGA PEVEHHGTA DLQVNTDFI PPPVDDGHG 
      SASLGLGYS PPSQGISDS YGAPLNSVT GSGAVVSSS 
      GDAHHDDES LSLGLSAAG IHSGQSHLS VIKSIGYEI 
      FPNTGGGLS HTGDTYSSP PLDSYSLDG PYAAAQAHK 
      SNGLSSFSF GGSIKSHGL RGGHGLIPP SGLYGAPPS 
      SHYGTPLLA KPTQQYGPP LNALKINPP KHPVIFREP 
      VPQGLIQSI GQHTAHKDA HGIIESTHS SSFSSGPTY 
      IPPPIPDVT KPVKEEEPP SPSGLYSLP HAHSAVSFQ 
      NVAHGSSFD FGSSHFGGG QPLTSYTAP LGSIDGSYG 
      LPVHSASGY SVAVDNAPS GGLTVGIDN SHPPVTIDL 
      TSATSNVGP SFAHLSHGY GYQNVHDCS SHKSQPLPS 
      LSYGVPSAN TYTASLSSL TTNIGGSYH GDLQTAHSQ 
      KVSTGVSYN AIHNSSEEK NYGKSLAQK FGPGAELIQ 
      SQSLDINNI PVQGSLGSY TLQIQSADG LNGRQSSSG 
      PVPHAQVLN DGLLHSILA AIEQPQSKN TKILGQPLA 
      KLQPSESYS GSFESDSDG QHSVTNAVA VPSPVTEAT 
      STTTTTQEP ESESETDTP LHLIDDNEI ALYFSNNLQ 
      DADNGSQKN DTSDGVLNG QQYGSYVSF KTPNASFVY 
      GNLPSDKSQ ERQDKD
    CDS sequence
    • >TC016058 RA
      atgtttgtt gcacttttg tgctcgtta ttggcaacg 
      caacaaatt gcagctgtc gtaaataat tcaaaatcg 
      gccagtata gaagaatcc agcaacaaa actgccagt 
      gccgcaaaa atacccgat tcagtttcg tctgcaagt 
      gaacaagag gcgaagcgt cgcgacgct cctctcagt 
      gatacatac ggcgccccc ttgagtggg ggaggaatc 
      gactcgtat gttccttca gggcccagt aaccccggc 
      ctgccgatt ccagtttac ggagtcccc gatgcaccc 
      tcaaataat attgtttac ccggcacca ccaccagac 
      atacctcca cctctcccc caaatatcc tccagttat 
      ggcgtccca ttcaaaact tacggccct ccgcagtta 
      atcaataat tttgacttg ggccactca accgatttc 
      gtgccgccg ccaccccca acaaagtac cttcccccc 
      atacggtcg cacaaaccc ttcagtgcc cccaaactg 
      ccgaggccg cgaccgcat tatgggccc ccacagaag 
      cccatcttc gtgccaaag ttgcctaaa cccaactac 
      gggccgccc aaatccgta tacggaccg ccaaagcaa 
      ccgctattt gtgccacag aaaaccaac tttaagcaa 
      cagtcggtt acgattgtt acggttccg tccaataat 
      tacggagtg ccgaatctt ggccatggg caagacgcg 
      ttcatctca ccaccggca gctataggg catggccat 
      tacggaccg cctgatccc gttccacac gcgcctcct 
      ccgggagta ccagcgccg cccacaccg ccggatatt 
      aagtacgat gggtggcag cccatcccc gggttggtg 
      tcaaggcca ccggatacg tacggcgcc cccgaggtt 
      gaacaccac ggaacggct gatttgcaa gtgaacact 
      gattttatc ccgcctcca gtggatgac ggccacggt 
      tcggcctca cttgggctg ggctacagc cccccatct 
      caaggaata agtgactcg tacggagcc cccttgaac 
      tcggtcacc gggtcgggg gctgtagtt agctcgtct 
      ggggacgca caccatgac gacgaatcc ctctctctt 
      ggtctgtcg gcggctggg attcactcc ggccaaagt 
      catctctca gtgatcaaa agcatagga tacgaaatt 
      ttccccaac actgggggt gggttgagt cacactggt 
      gacacctac agctcacca ccgcttgat tcgtactcg 
      cttgatggc ccatatgcc gctgctcag gctcataag 
      tccaatggg ttgtcgagt ttctcgttt ggtggttca 
      ataaaatca catggtttg cgtggaggc cacggttta 
      attcctccc agtggtctt tacggagct ccgccgagt 
      agccattac gggactccc cttttggcg aaacccact 
      caacaatat ggtcccccc ttgaacgct ctgaagatc 
      aatccgccc aaacatccc gttattttc cgagaacca 
      gtccctcag ggtttgatt caatcaatc ggtcaacac 
      acggctcac aaagatgct cacggtatt attgagagt 
      actcattct agtagtttc tcctcggga cccacctat 
      attcctccc ccaattccc gatgtgacg aaacccgtg 
      aaggaggaa gaaccacct tcaccgtcc ggtttgtac 
      agcttgccc catgcacat tcggcagtt tctttccaa 
      aatgtagct catggttcg tcgtttgat ttcgggtca 
      agccatttt ggtggcggt cagcctttg acgagctac 
      acggctcca ttggggagt atagatggg tcgtacggt 
      ttaccggta cactcagct tctggctat tcagtcgca 
      gtggacaat gcaccatct ggtggcttg accgtggga 
      atagacaat tctcaccct cctgtgacc attgattta 
      acttctgcc acgagtaat gtgggccca agcttcgcc 
      cacttatcc catggttat ggttaccaa aacgtccat 
      gactgtagt tcacacaaa tcgcaacct ttaccgtca 
      ttatcttac ggtgtccca tctgctaat acttatacc 
      gcgtctttg tcgtctttg accaccaat atcggtgga 
      tcttatcac ggtgatctt caaactgcc cattcgcaa 
      aaagtttca accggggtt tcgtacaat gcgatccat 
      aattcatca gaagaaaaa aattacggg aaatcgctc 
      gcgcaaaaa ttcgggccc ggagctgag ttaattcag 
      tcgcaaagc ctcgacatt aataatatc ccagtccaa 
      gggtctttg ggtagctat actttgcaa atccagtca 
      gcggatggt ttaaacggt cgtcagtcg tcttccggt 
      cccgttccg cacgctcaa gttttaaac gatggtctg 
      ctacactca attctggcg gctatcgag caaccgcag 
      agcaaaaac acgaaaatt ttgggccag cctttggca 
      aaattgcag ccgagcgag agttacagc ggatctttt 
      gagtccgac tcagacggg cagcattcc gtaacaaat 
      gccgttgcc gttccatca ccggtaacg gaagcaact 
      tcaacaaca acgactacg caagaacca gaatcggag 
      tctgaaacg gatactcca ctccactta atagacgac 
      aacgagatt gcattatac ttcagcaac aatttgcaa 
      gatgcagat aatggctct cagaaaaac gatacaagt 
      gatggcgtg ctaaacggt cagcagtac ggaagttac 
      gtttcgttt aaaacgccg aatgctagt ttcgtgtac 
      ggaaactta ccaagtgac aagtcgcaa gaacgacaa 
      gacaaggat tag
    mRNA sequence
    • >TC016058 RA
      caccggaat tatttttcg aaaacgcac cgatatgct 
      cgcacgtta ggttattag tgtagtata gtggtagtg 
      agtggtaac ttaaggata acacgaaca cttaaggat 
      tacgatgac gatacaaca gatgtttgt tgcactttt 
      gtgctcgtt attggcaac gcaacaaat tgcagctgt 
      cgtaaataa ttcaaaatc ggccagtat agaagaatc 
      cagcaacaa aactgccag tgccgcaaa aatacccga 
      ttcagtttc gtctgcaag tgaacaaga ggcgaagcg 
      tcgcgacgc tcctctcag tgatacata cggcgcccc 
      cttgagtgg gggaggaat cgactcgta tgttccttc 
      agggcccag taaccccgg cctgccgat tccagttta 
      cggagtccc cgatgcacc ctcaaataa tattgttta 
      cccggcacc accaccaga catacctcc acctctccc 
      ccaaatatc ctccagtta tggcgtccc attcaaaac 
      ttacggccc tccgcagtt aatcaataa ttttgactt 
      gggccactc aaccgattt cgtgccgcc gccaccccc 
      aacaaagta ccttccccc catacggtc gcacaaacc 
      cttcagtgc ccccaaact gccgaggcc gcgaccgca 
      ttatgggcc cccacagaa gcccatctt cgtgccaaa 
      gttgcctaa acccaacta cgggccgcc caaatccgt 
      atacggacc gccaaagca accgctatt tgtgccaca 
      gaaaaccaa ctttaagca acagtcggt tacgattgt 
      tacggttcc gtccaataa ttacggagt gccgaatct 
      tggccatgg gcaagacgc gttcatctc accaccggc 
      agctatagg gcatggcca ttacggacc gcctgatcc 
      cgttccaca cgcgcctcc tccgggagt accagcgcc 
      gcccacacc gccggatat taagtacga tgggtggca 
      gcccatccc cgggttggt gtcaaggcc accggatac 
      gtacggcgc ccccgaggt tgaacacca cggaacggc 
      tgatttgca agtgaacac tgattttat cccgcctcc 
      agtggatga cggccacgg ttcggcctc acttgggct 
      gggctacag ccccccatc tcaaggaat aagtgactc 
      gtacggagc ccccttgaa ctcggtcac cgggtcggg 
      ggctgtagt tagctcgtc tggggacgc acaccatga 
      cgacgaatc cctctctct tggtctgtc ggcggctgg 
      gattcactc cggccaaag tcatctctc agtgatcaa 
      aagcatagg atacgaaat tttccccaa cactggggg 
      tgggttgag tcacactgg tgacaccta cagctcacc 
      accgcttga ttcgtactc gcttgatgg cccatatgc 
      cgctgctca ggctcataa gtccaatgg gttgtcgag 
      tttctcgtt tggtggttc aataaaatc acatggttt 
      gcgtggagg ccacggttt aattcctcc cagtggtct 
      ttacggagc tccgccgag tagccatta cgggactcc 
      ccttttggc gaaacccac tcaacaata tggtccccc 
      cttgaacgc tctgaagat caatccgcc caaacatcc 
      cgttatttt ccgagaacc agtccctca gggtttgat 
      tcaatcaat cggtcaaca cacggctca caaagatgc 
      tcacggtat tattgagag tactcattc tagtagttt 
      ctcctcggg acccaccta tattcctcc cccaattcc 
      cgatgtgac gaaacccgt gaaggagga agaaccacc 
      ttcaccgtc cggtttgta cagcttgcc ccatgcaca 
      ttcggcagt ttctttcca aaatgtagc tcatggttc 
      gtcgtttga tttcgggtc aagccattt tggtggcgg 
      tcagccttt gacgagcta cacggctcc attggggag 
      tatagatgg gtcgtacgg tttaccggt acactcagc 
      ttctggcta ttcagtcgc agtggacaa tgcaccatc 
      tggtggctt gaccgtggg aatagacaa ttctcaccc 
      tcctgtgac cattgattt aacttctgc cacgagtaa 
      tgtgggccc aagcttcgc ccacttatc ccatggtta 
      tggttacca aaacgtcca tgactgtag ttcacacaa 
      atcgcaacc tttaccgtc attatctta cggtgtccc 
      atctgctaa tacttatac cgcgtcttt gtcgtcttt 
      gaccaccaa tatcggtgg atcttatca cggtgatct 
      tcaaactgc ccattcgca aaaagtttc aaccggggt 
      ttcgtacaa tgcgatcca taattcatc agaagaaaa 
      aaattacgg gaaatcgct cgcgcaaaa attcgggcc 
      cggagctga gttaattca gtcgcaaag cctcgacat 
      taataatat cccagtcca agggtcttt gggtagcta 
      tactttgca aatccagtc agcggatgg tttaaacgg 
      tcgtcagtc gtcttccgg tcccgttcc gcacgctca 
      agttttaaa cgatggtct gctacactc aattctggc 
      ggctatcga gcaaccgca gagcaaaaa cacgaaaat 
      tttgggcca gcctttggc aaaattgca gccgagcga 
      gagttacag cggatcttt tgagtccga ctcagacgg 
      gcagcattc cgtaacaaa tgccgttgc cgttccatc 
      accggtaac ggaagcaac ttcaacaac aacgactac 
      gcaagaacc agaatcgga gtctgaaac ggatactcc 
      actccactt aatagacga caacgagat tgcattata 
      cttcagcaa caatttgca agatgcaga taatggctc 
      tcagaaaaa cgatacaag tgatggcgt gctaaacgg 
      tcagcagta cggaagtta cgtttcgtt taaaacgcc 
      gaatgctag tttcgtgta cggaaactt accaagtga 
      caagtcgca agaacgaca agacaagga ttagtgtgt 
      cgaatctgc tcaagaact ttttagtca tgaactgag 
      ttccactca aatgaagtt ggagtggag ttcgggtat 
      tgttattta tgcgcggct cactttcga ggcctagaa 
      gcctctaag gcgatctga tattattag tgatcgaaa 
      taatttatt gtatgttta attgataga ttaaggaga 
      aaaaactat gtacaaaag ttttttaaa aactaataa 
      aacatgcct ggaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY