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Gene information
General information
TC016044 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450445.1: 0..1794
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016044
    iBeetle screen iB_05949

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016044 RA
      VSKPPTLKL KMRCLFSII LVLLLSHEG TTLECAIPP 
      EPRVRFFDS IIHYEGNRI PFSVLRAGW QGSDVQFTF 
      TTINKLTKN IFVTYDSQA IISLKLSGK QIKEVEDGA 
      FVTLVCLQE LLLDRNNIT KLAKNIFTD LERLEKLDL 
      SNNRIEVID DFVFSQLAS LKILNLGNN RIEKLQNLA 
      FANLTKLEY LNLERNEIG KIAPDLFLP LTSLSNLTL 
      AWNMLNVEP EKWHGLAHL HFLDLAANN LDHFDPSYN 
      FTFRNLRGL NLSLNSLSQ LNVFEIKRH LPRLELIDL 
      NGNAWFCDD LERIVRELN DSRIKAAPG NHTRGVRGI 
      ACSEVPGYS TRGTTLIVT TSVKTPTWV KKDELERYV 
      ERKIANNSE KIESSIATL RSFVVFVFA LVCLFILFE 
      ILVRLDCCR SVIGRLRGG NELYVDNND VENFRLLGR
    CDS sequence
    • >TC016044 RA
      gtttccaaa ccaccgacg ctaaaactc aagatgcgt 
      tgcttattt tcaattatt ttggtgcta cttttgtcc 
      cacgagggc acaaccctc gagtgtgcg atccctcca 
      gaaccacga gtgcgtttt ttcgattcg ataattcac 
      tatgaggga aatcgcatc cctttcagt gttctcaga 
      gcgggctgg caaggcagt gacgtccag ttcacattt 
      accacaata aacaaactc acgaaaaat atttttgtc 
      acttatgat tcacaagcg ataatttcg ctaaaactg 
      agcggaaaa caaatcaaa gaagtggaa gacggggcc 
      tttgtgacg cttgtttgc ctccaagag ctcctccta 
      gaccgtaac aatatcaca aagttggca aaaaatatt 
      ttcaccgac ttggaacgc ttggaaaag cttgacttg 
      agcaacaat cgcatcgaa gtcatcgac gattttgtg 
      ttctcccag ttggcaagc ctgaagatc ctgaatttg 
      gggaataac cgaatcgag aaattgcaa aatttggcg 
      tttgccaac ctcacaaaa ttggaatat ttgaatttg 
      gaacgcaac gaaattggc aaaattgcg cccgattta 
      tttctcccg ttgacaagt ttgagtaat ttgactttg 
      gcgtggaac atgttgaat gtggagccg gagaaatgg 
      cacggtttg gcacatttg cattttctg gacttggct 
      gcgaacaac ttggaccat tttgacccg agttacaat 
      ttcacgttt cgcaatttg cgcggttta aatttgagc 
      ttgaattct ctgagtcag ttgaacgtt ttcgaaatc 
      aagcggcac ttgccccgg cttgagttg atcgatttg 
      aacggaaat gcgtggttc tgcgacgat ctggagcga 
      attgttcgc gaactgaac gattcaagg atcaaagca 
      gcgccgggg aatcacaca aggggagtg cgcgggatt 
      gcctgcagt gaagtaccg gggtattca accaggggt 
      accactctg attgtcaca actagcgtg aaaaccccg 
      acttgggtg aaaaaggac gagctggag cgatacgtc 
      gagcggaaa attgcaaat aacagtgag aaaatcgag 
      agctcgatt gcgacgttg cgaagcttt gttgttttc 
      gtgtttgcg ctcgtttgt ctttttatc ttgtttgaa 
      attttagta cggcttgat tgttgccgg agtgttatc 
      gggaggctc cggggaggc aatgaactg tatgttgat 
      aacaatgac gttgaaaat tttagactg ttaggcaga 
      taa
    mRNA sequence
    • >TC016044 RA
      ttgtttcca aaccaccga cgctaaaac tcaagatgc 
      gttgcttat tttcaatta ttttggtgc tacttttgt 
      cccacgagg gcacaaccc tcgagtgtg cgatccctc 
      cagaaccac gagtgcgtt ttttcgatt cgataattc 
      actatgagg gaaatcgca tccctttca gtgttctca 
      gagcgggct ggcaaggca gtgacgtcc agttcacat 
      ttaccacaa taaacaaac tcacgaaaa atatttttg 
      tcacttatg attcacaag cgataattt cgctaaaac 
      tgagcggaa aacaaatca aagaagtgg aagacgggg 
      cctttgtga cgcttgttt gcctccaag agctcctcc 
      tagaccgta acaatatca caaagttgg caaaaaata 
      ttttcaccg acttggaac gcttggaaa agcttgact 
      tgagcaaca atcgcatcg aagtcatcg acgattttg 
      tgttctccc agttggcaa gcctgaaga tcctgaatt 
      tggggaata accgaatcg agaaattgc aaaatttgg 
      cgtttgcca acctcacaa aattggaat atttgaatt 
      tggaacgca acgaaattg gcaaaattg cgcccgatt 
      tatttctcc cgttgacaa gtttgagta atttgactt 
      tggcgtgga acatgttga atgtggagc cggagaaat 
      ggcacggtt tggcacatt tgcattttc tggacttgg 
      ctgcgaaca acttggacc attttgacc cgagttaca 
      atttcacgt ttcgcaatt tgcgcggtt taaatttga 
      gcttgaatt ctctgagtc agttgaacg ttttcgaaa 
      tcaagcggc acttgcccc ggcttgagt tgatcgatt 
      tgaacggaa atgcgtggt tctgcgacg atctggagc 
      gaattgttc gcgaactga acgattcaa ggatcaaag 
      cagcgccgg ggaatcaca caaggggag tgcgcggga 
      ttgcctgca gtgaagtac cggggtatt caaccaggg 
      gtaccactc tgattgtca caactagcg tgaaaaccc 
      cgacttggg tgaaaaagg acgagctgg agcgatacg 
      tcgagcgga aaattgcaa ataacagtg agaaaatcg 
      agagctcga ttgcgacgt tgcgaagct ttgttgttt 
      tcgtgtttg cgctcgttt gtcttttta tcttgtttg 
      aaattttag tacggcttg attgttgcc ggagtgtta 
      tcgggaggc tccggggag gcaatgaac tgtatgttg 
      ataacaatg acgttgaaa attttagac tgttaggca 
      gataattcg tttcgttca cctattatt gcctgtgat 
      gtgatattt ttgtaccgt tttttttct gaataaatt 
      ttgtgataa aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY