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Gene information
General information
TC016023 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451234.1: 0..5413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016023
    iBeetle screen iB_05943

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016023 RA
      MLPTVQKIK MAQWGTTSE SGILRKNKP KENHDDLLV 
      FGYACKLFR DDEKASYID KGHHLIPWM GDDSLKIDR 
      YDCRGALSD ISPFEASRE GFDAMRWLG LSDSERKLE 
      QLCDKERYY SLHINEEEE QMYKEEEQK RQKANEFQY 
      SYDVPANPA PESAPTVAE PEEEDEEYV PSPHLDVPV 
      DIAIPKTVK ENIRIEKTA LFVCKQGPQ MEILIKAKQ 
      ADNPQFGFL NQGHNLYKY YRHVLAAIK SGRYQVTST 
      TKKEEKREE PPEASSTDD HYLHPSLIS TSSSQPDVQ 
      TTIPTIPYK PSANCSYSQ LVNRIQGNS QSEGAKPPE 
      MTYDQQQYY QYYYAAQYY EYYKQLAQY QQGNGQGAS 
      IQPPETHLD PSVQTYIQQ MAYAQYMHN QSSSTNPYA 
      QIVSNVSKE MSNPYPTDE KVPKTTEPD AESVETPKS 
      TLLLLAQYG SDSEPEEDD SFKTPTGEV QIVIDKMAT 
      YVAKNGVQF EEIVKAKGD PRFEFLNES HEFYKYYKN 
      KIKLITEGE GSGDKGAGT PPKPKEKKV IAPCASG
    CDS sequence
    • >TC016023 RA
      atgttgcca accgtacaa aaaatcaaa atggcgcag 
      tggggcaca acttcagag tcgggtatt ttgcgcaaa 
      aacaagccg aaagagaac cacgacgat cttttagtg 
      ttcggttat gcgtgtaaa ttgttccgc gatgacgaa 
      aaggcctcc tacatcgat aaaggccac catcttatc 
      ccgtggatg ggagacgac agtctcaaa attgataga 
      tatgactgt cggggcgcc ctgtcggac atatcaccg 
      tttgaggcg agtcgggag ggttttgac gccatgcgg 
      tggttgggt ttgagtgac tctgagagg aaacttgag 
      cagttgtgt gataaggaa cggtactat tcgttacat 
      atcaatgaa gaagaggag caaatgtac aaggaggag 
      gagcagaag agacagaaa gcgaatgag tttcagtac 
      agttatgac gtcccggcg aacccggcg ccagagagc 
      gcccccacg gtggcagag cccgaggag gaggacgag 
      gagtacgtc ccgtcaccg catttggac gtgccagtt 
      gatattgca ataccaaaa accgtcaaa gaaaatatt 
      cggatcgag aaaacggcg cttttcgta tgtaaacaa 
      gggccccaa atggaaatc ctaatcaag gccaaacaa 
      gctgataat ccacaattt ggttttctg aaccaggga 
      cacaacttg tacaagtat taccggcac gtgttggcg 
      gctatcaaa agtggccgg tatcaagtt acttcaacc 
      acaaaaaaa gaggaaaag cgtgaagag ccgcctgaa 
      gcaagttcg accgatgat cactacctt cacccaagc 
      ttaatatcg acgtcaagc agccaaccg gatgtacaa 
      actacaata ccgacaata ccgtacaag ccttcggcc 
      aattgttcc tattcacag ttggttaac aggatccag 
      ggaaacagc caatcggaa ggcgccaag ccgccggaa 
      atgacctac gaccagcag cagtattat cagtattac 
      tacgcagct cagtactat gagtattac aagcagctg 
      gcgcagtac cagcaaggg aatggacaa ggtgcgagc 
      attcaaccg cctgagacg cacttggac cccagcgtt 
      caaacctac atccaacag atggcgtac gctcaatac 
      atgcacaac caatcatca agcaccaat ccctacgcc 
      cagatcgtc tcgaacgtt agtaaagaa atgtcaaat 
      ccgtatcca acggacgaa aaagtgcca aaaacaaca 
      gaaccggac gccgaaagc gtcgaaacg cccaaaagt 
      acgctcctt ctactagct cagtatggt tccgattcg 
      gaaccggaa gaagacgac agttttaaa accccaacc 
      ggggaagta cagatcgtg attgacaaa atggcaacg 
      tacgtggcc aaaaacggc gtccagttt gaggaaatt 
      gtcaaggct aagggggat ccccgattt gagtttctc 
      aacgaaagt catgaattt tataagtat tacaagaat 
      aagatcaag ttgattacg gagggcgag ggcagcggg 
      gacaagggg gcggggacg cctcccaaa cccaaggag 
      aaaaaagtt atagcaccc tgcgcttcc ggttga
    mRNA sequence
    • >TC016023 RA
      tattcgtaa aattgtcca ttatttggt tttgcgtat 
      tgttggttg ccggggcaa ccggcattg tttgtaaaa 
      aatgttgcc aaccgtaca aaaaatcaa aatggcgca 
      gtggggcac aacttcaga gtcgggtat tttgcgcaa 
      aaacaagcc gaaagagaa ccacgacga tcttttagt 
      gttcggtta tgcgtgtaa attgttccg cgatgacga 
      aaaggcctc ctacatcga taaaggcca ccatcttat 
      cccgtggat gggagacga cagtctcaa aattgatag 
      atatgactg tcggggcgc cctgtcgga catatcacc 
      gtttgaggc gagtcggga gggttttga cgccatgcg 
      gtggttggg tttgagtga ctctgagag gaaacttga 
      gcagttgtg tgataagga acggtacta ttcgttaca 
      tatcaatga agaagagga gcaaatgta caaggagga 
      ggagcagaa gagacagaa agcgaatga gtttcagta 
      cagttatga cgtcccggc gaacccggc gccagagag 
      cgcccccac ggtggcaga gcccgagga ggaggacga 
      ggagtacgt cccgtcacc gcatttgga cgtgccagt 
      tgatattgc aataccaaa aaccgtcaa agaaaatat 
      tcggatcga gaaaacggc gcttttcgt atgtaaaca 
      agggcccca aatggaaat cctaatcaa ggccaaaca 
      agctgataa tccacaatt tggttttct gaaccaggg 
      acacaactt gtacaagta ttaccggca cgtgttggc 
      ggctatcaa aagtggccg gtatcaagt tacttcaac 
      cacaaaaaa agaggaaaa gcgtgaaga gccgcctga 
      agcaagttc gaccgatga tcactacct tcacccaag 
      cttaatatc gacgtcaag cagccaacc ggatgtaca 
      aactacaat accgacaat accgtacaa gccttcggc 
      caattgttc ctattcaca gttggttaa caggatcca 
      gggaaacag ccaatcgga aggcgccaa gccgccgga 
      aatgaccta cgaccagca gcagtatta tcagtatta 
      ctacgcagc tcagtacta tgagtatta caagcagct 
      ggcgcagta ccagcaagg gaatggaca aggtgcgag 
      cattcaacc gcctgagac gcacttgga ccccagcgt 
      tcaaaccta catccaaca gatggcgta cgctcaata 
      catgcacaa ccaatcatc aagcaccaa tccctacgc 
      ccagatcgt ctcgaacgt tagtaaaga aatgtcaaa 
      tccgtatcc aacggacga aaaagtgcc aaaaacaac 
      agaaccgga cgccgaaag cgtcgaaac gcccaaaag 
      tacgctcct tctactagc tcagtatgg ttccgattc 
      ggaaccgga agaagacga cagttttaa aaccccaac 
      cggggaagt acagatcgt gattgacaa aatggcaac 
      gtacgtggc caaaaacgg cgtccagtt tgaggaaat 
      tgtcaaggc taaggggga tccccgatt tgagtttct 
      caacgaaag tcatgaatt ttataagta ttacaagaa 
      taagatcaa gttgattac ggagggcga gggcagcgg 
      ggacaaggg ggcggggac gcctcccaa acccaagga 
      gaaaaaagt tatagcacc ctgcgcttc cggttgaag 
      aaagtgacg aagaagaga agctcacga ctgaaacaa 
      ctgattcag ttacaaaag aagcagaat tgtcaaatg 
      atacgtcgg agtctagtc acaaaaacg gggttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY