Status
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Gene information
General information
TC031853
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016013,TC016016,TC016017,TC016018
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451946.1: 3127..145371
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031853
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05942

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium not present - Number of animals showing the phenotype: 4

    tropharium shape irregular - Number of animals showing the phenotype: 4

    tropharium size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031853 RA
      MYPWYRDSV GGQLGQLCG PMATTIKTE NGYNDCMLA 
      LDYATQSKK AFGWDGAQS GGSPSGPQS GGVGGQGAQ 
      GLVHWMSVM AEHMDPAMS HYMSWNQEC GQHAKDDYP 
      NWTRNTMGI NKQGYEAKM NEHNQMHQK TLGYASSCY 
      FQPPTSKKN LDDHHMGGT PGLYTSGRS TSSSSSPGV 
      AGSSPALLV VPQPINATK MGGMQNGGP QPRKYQCKM 
      CPQYSFCYH SERSFFSFF FAVIVIYFF TERVTSACN 
      NRKKVKKKW SMDGRLEYR TGGLDFHQP SPMLLDNHQ 
      NYSSHLQRS IDTLRSLGD RLGERPMDL NLSLMQERM 
      NLDLNHLQE RPLMDRNNL ERGYERLRE PQVDMRHED 
      LKYREYKAH LENLRSESS RSMEGRTDE DRGTTPSTP 
      PTPISVGEN FQEEKVFGS KAELQLHTQ AHMREAKPY 
      KCSQCCKAF ANSSYLSQH TRIHLGIKP YRCEICQRK 
      FTQLSHLQQ HIRTHTGDK PYKCRHPGC QKAFSQLSN 
      LQSHSRCHQ TDKPYKCNS CYKCFSDEP SLLEHIPKH 
      KESKHLKTH ICQYCGKSY TQETYLSKH MQKHAERTD 
      KRPPIGPAL GLNHRGLEH PYWPKVSPD SAENMHEYP 
      SDIPRQILE QNEDLVIIR QQLQNPPAP PGTPTANLS 
      NSYDASISK STTNSAFTP INSMSGHLN LNHHQLAPN 
      RPYIYDAIS FQKQNSLDL PKSQPSNGF PNQLISLHQ 
      IRNYAHQPN AGLMEHSIL GGLKDK
    • >TC031853 RB
      MYPWYRDSV GGQLGQLCG PMATTIKTE NGYNDCMLA 
      LDYATQSKK AFGWDGAQS GGSPSGPQS GGVGGQGAQ 
      GLVHWMSVM AEHMDPAMS HYMSWNQEC GQHAKDDYP 
      NWTRNTMGI NKQGYEAKM NEHNQMHQK NLDDHHMGG 
      TPGLYTSGR STSSSSSPG VAGSSPALL VVPQPINAT 
      KMGGMQNGG PQPRKYQCK MCPQYSFCY HSERSFFSF 
      FFAVIVIYF FTERVTSAC NNRKKVKKK WSMDGRLEY 
      RTGGLDFHQ PSPMLLDNH QNYSSHLQR SIDTLRSLG 
      DRLGERPMD LNLSLMQER MNLDLNHLQ ERPLMDRNN 
      LERGYERLR EPQVDMRHE DLKYREYKA HLENLRSES 
      SRSMEGRTD EDRGTTPST PPTPISVGE NFQEEKVFG 
      SKAELQLHT QAHMREAKP YKCSQCCKA FANSSYLSQ 
      HTRIHLGIK PYRCEICQR KFTQLSHLQ QHIRTHTGD 
      KPYKCRHPG CQKAFSQLS NLQSHSRCH QTDKPYKCN 
      SCYKCFSDE PSLLEHIPK HKESKHLKT HICQYCGKS 
      YTQETYLSK HMQKHAERT DKRPPIGPA LGLNHRGLE 
      HPYWPKVSP DSAENMHEY PSDIPRQIL EQNEDLVII 
      RQQLQNPPA PPGTPTANL SNSYDASIS KSTTNSAFT 
      PINSMSGHL NLNHHQLAP NRPYIYDAI SFQKQNSLD 
      LPKSQPSNG FPNQLISLH QIRNYAHQP NAGLMEHSI 
      LGGLKDK
    CDS sequence
    • >TC031853 RA
      atgtatcct tggtaccgt gactccgtt ggcggtcaa 
      ttagggcaa ttatgcggc ccgatggcc accacaatc 
      aaaaccgaa aacggctac aacgactgc atgctggcc 
      ctcgactac gccacgcag agtaagaaa gcattcggc 
      tgggacggg gcccaatcg ggtggcagc ccctcgggg 
      ccgcagtcg gggggcgtg ggaggccag ggggcccaa 
      ggactcgtc cactggatg agtgttatg gctgagcat 
      atggatcca gcgatgtcg cattacatg tcctggaat 
      caagagtgc ggccaacat gccaaagac gattacccc 
      aattggacg aggaacacg atgggcatc aataagcag 
      gggtacgag gccaagatg aacgaacat aatcagatg 
      caccagaaa actctcggc tacgcctcg agctgctat 
      ttccaacca ccaacgtca aagaaaaac ctggacgac 
      caccacatg gggggcacg ccgggcctg tacacgtcg 
      gggcgttcg acgtcgtcg agctctagc ccgggggtg 
      gccggctcg agtccggcc ctgttggtg gtgccccag 
      cccatcaac gcgaccaaa atgggcgga atgcaaaac 
      ggcggcccg caaccacgg aagtaccaa tgtaaaatg 
      tgcccgcag tatagtttt tgctaccat tcggagcgc 
      tcgtttttt tcttttttt ttcgcggtt attgtcatt 
      tattttttc accgagcga gtgaccagt gcttgtaat 
      aatcgaaaa aaagtaaaa aaaaagtgg tcgatggat 
      ggccgattg gagtaccgc accggcggc ctggacttc 
      caccagccg tcgccgatg ctcctcgac aaccaccag 
      aactactcc tcgcacctg cagcgctcc atcgacacg 
      ctgcgctcg ctgggcgac cgcctgggg gagcggccc 
      atggacctc aacctcagc ctgatgcag gagcgcatg 
      aacctggac ctcaaccac ctgcaggag cggccgctc 
      atggacagg aacaacctg gagcgcggc tacgagcgg 
      ctgcgcgag ccccaggtg gacatgcgg cacgaggac 
      ttgaagtac agggagtac aaggcgcac ctggagaac 
      ctccgctcg gagagttca aggtcgatg gaagggagg 
      acggacgag gaccggggc acgacgccg tcgacgccg 
      cccacgccg atctcggtc ggggagaac ttccaagag 
      gaaaaggtt ttcgggtca aaggccgag ttgcaattg 
      cacacgcaa gctcatatg cgcgaagcc aaaccgtac 
      aagtgttcg cagtgttgt aaggcgttc gccaacagt 
      tcctattta tcgcagcac actaggatc cacttaggt 
      atcaaaccg taccgatgc gaaatctgc caaaggaag 
      ttcacacaa ctcagccat ttgcaacaa cacattcgt 
      acgcatacg ggcgataaa ccctacaaa tgtcgacat 
      ccaggttgt cagaaggcg ttctcgcag ctgagcaat 
      ttgcagtcg cattcgagg tgtcatcaa acggacaaa 
      ccgtacaaa tgcaattcg tgctacaag tgcttcagc 
      gacgagccg tccctactc gaacacatt cccaaacat 
      aaagagagc aagcattta aagacgcac atatgtcag 
      tattgtggc aagagctat acgcaggag acctatctc 
      agtaaacat atgcaaaaa cacgcggaa aggacggac 
      aagagaccg ccgataggg cccgcgcta ggattgaac 
      caccgaggc ttggagcac ccctactgg ccgaaagtg 
      agccccgac agcgctgag aacatgcac gagtacccc 
      tctgacatc ccccggcag atcctcgag cagaacgag 
      gacctggtg atcatccgg cagcagctg cagaacccg 
      cccgcgccc cccggcacc cccaccgcc aacctgtcg 
      aacagctac gacgcctcg atatcgaaa tccaccacc 
      aactcggcc ttcaccccc atcaactcc atgtcgggc 
      cacctcaac ctcaaccac caccagctg gcccccaac 
      aggccctac atctacgac gccatcagc ttccagaag 
      cagaacagt ctcgacctg cccaagagc cagccctcc 
      aacggcttc cccaaccag ctgatctcc ctccaccag 
      atccggaac tacgcccac cagcccaac gccggcctc 
      atggagcac tccatcctg ggcgggctc aaggacaag 
      tag
    • >TC031853 RB
      atgtatcct tggtaccgt gactccgtt ggcggtcaa 
      ttagggcaa ttatgcggc ccgatggcc accacaatc 
      aaaaccgaa aacggctac aacgactgc atgctggcc 
      ctcgactac gccacgcag agtaagaaa gcattcggc 
      tgggacggg gcccaatcg ggtggcagc ccctcgggg 
      ccgcagtcg gggggcgtg ggaggccag ggggcccaa 
      ggactcgtc cactggatg agtgttatg gctgagcat 
      atggatcca gcgatgtcg cattacatg tcctggaat 
      caagagtgc ggccaacat gccaaagac gattacccc 
      aattggacg aggaacacg atgggcatc aataagcag 
      gggtacgag gccaagatg aacgaacat aatcagatg 
      caccagaaa aacctggac gaccaccac atggggggc 
      acgccgggc ctgtacacg tcggggcgt tcgacgtcg 
      tcgagctct agcccgggg gtggccggc tcgagtccg 
      gccctgttg gtggtgccc cagcccatc aacgcgacc 
      aaaatgggc ggaatgcaa aacggcggc ccgcaacca 
      cggaagtac caatgtaaa atgtgcccg cagtatagt 
      ttttgctac cattcggag cgctcgttt ttttctttt 
      tttttcgcg gttattgtc atttatttt ttcaccgag 
      cgagtgacc agtgcttgt aataatcga aaaaaagta 
      aaaaaaaag tggtcgatg gatggccga ttggagtac 
      cgcaccggc ggcctggac ttccaccag ccgtcgccg 
      atgctcctc gacaaccac cagaactac tcctcgcac 
      ctgcagcgc tccatcgac acgctgcgc tcgctgggc 
      gaccgcctg ggggagcgg cccatggac ctcaacctc 
      agcctgatg caggagcgc atgaacctg gacctcaac 
      cacctgcag gagcggccg ctcatggac aggaacaac 
      ctggagcgc ggctacgag cggctgcgc gagccccag 
      gtggacatg cggcacgag gacttgaag tacagggag 
      tacaaggcg cacctggag aacctccgc tcggagagt 
      tcaaggtcg atggaaggg aggacggac gaggaccgg 
      ggcacgacg ccgtcgacg ccgcccacg ccgatctcg 
      gtcggggag aacttccaa gaggaaaag gttttcggg 
      tcaaaggcc gagttgcaa ttgcacacg caagctcat 
      atgcgcgaa gccaaaccg tacaagtgt tcgcagtgt 
      tgtaaggcg ttcgccaac agttcctat ttatcgcag 
      cacactagg atccactta ggtatcaaa ccgtaccga 
      tgcgaaatc tgccaaagg aagttcaca caactcagc 
      catttgcaa caacacatt cgtacgcat acgggcgat 
      aaaccctac aaatgtcga catccaggt tgtcagaag 
      gcgttctcg cagctgagc aatttgcag tcgcattcg 
      aggtgtcat caaacggac aaaccgtac aaatgcaat 
      tcgtgctac aagtgcttc agcgacgag ccgtcccta 
      ctcgaacac attcccaaa cataaagag agcaagcat 
      ttaaagacg cacatatgt cagtattgt ggcaagagc 
      tatacgcag gagacctat ctcagtaaa catatgcaa 
      aaacacgcg gaaaggacg gacaagaga ccgccgata 
      gggcccgcg ctaggattg aaccaccga ggcttggag 
      cacccctac tggccgaaa gtgagcccc gacagcgct 
      gagaacatg cacgagtac ccctctgac atcccccgg 
      cagatcctc gagcagaac gaggacctg gtgatcatc 
      cggcagcag ctgcagaac ccgcccgcg ccccccggc 
      acccccacc gccaacctg tcgaacagc tacgacgcc 
      tcgatatcg aaatccacc accaactcg gccttcacc 
      cccatcaac tccatgtcg ggccacctc aacctcaac 
      caccaccag ctggccccc aacaggccc tacatctac 
      gacgccatc agcttccag aagcagaac agtctcgac 
      ctgcccaag agccagccc tccaacggc ttccccaac 
      cagctgatc tccctccac cagatccgg aactacgcc 
      caccagccc aacgccggc ctcatggag cactccatc 
      ctgggcggg ctcaaggac aagtag
    mRNA sequence
    • >TC031853 RA
      gcgttgaaa tcaccacaa gtgcgttca gttcaccgg 
      aacccggaa ttcagcccg gaaccggcg gtcaagttc 
      aaaggcgtg attatctcc gtcgtcgaa gatgtatcc 
      ttggtaccg tgactccgt tggcggtca attagggca 
      attatgcgg cccgatggc caccacaat caaaaccga 
      aaacggcta caacgactg catgctggc cctcgacta 
      cgccacgca gagtaagaa agcattcgg ctgggacgg 
      ggcccaatc gggtggcag cccctcggg gccgcagtc 
      ggggggcgt gggaggcca gggggccca aggactcgt 
      ccactggat gagtgttat ggctgagca tatggatcc 
      agcgatgtc gcattacat gtcctggaa tcaagagtg 
      cggccaaca tgccaaaga cgattaccc caattggac 
      gaggaacac gatgggcat caataagca ggggtacga 
      ggccaagat gaacgaaca taatcagat gcaccagaa 
      aactctcgg ctacgcctc gagctgcta tttccaacc 
      accaacgtc aaagaaaaa cctggacga ccaccacat 
      ggggggcac gccgggcct gtacacgtc ggggcgttc 
      gacgtcgtc gagctctag cccgggggt ggccggctc 
      gagtccggc cctgttggt ggtgcccca gcccatcaa 
      cgcgaccaa aatgggcgg aatgcaaaa cggcggccc 
      gcaaccacg gaagtacca atgtaaaat gtgcccgca 
      gtatagttt ttgctacca ttcggagcg ctcgttttt 
      ttctttttt tttcgcggt tattgtcat ttatttttt 
      caccgagcg agtgaccag tgcttgtaa taatcgaaa 
      aaaagtaaa aaaaaagtg gtcgatgga tggccgatt 
      ggagtaccg caccggcgg cctggactt ccaccagcc 
      gtcgccgat gctcctcga caaccacca gaactactc 
      ctcgcacct gcagcgctc catcgacac gctgcgctc 
      gctgggcga ccgcctggg ggagcggcc catggacct 
      caacctcag cctgatgca ggagcgcat gaacctgga 
      cctcaacca cctgcagga gcggccgct catggacag 
      gaacaacct ggagcgcgg ctacgagcg gctgcgcga 
      gccccaggt ggacatgcg gcacgagga cttgaagta 
      cagggagta caaggcgca cctggagaa cctccgctc 
      ggagagttc aaggtcgat ggaagggag gacggacga 
      ggaccgggg cacgacgcc gtcgacgcc gcccacgcc 
      gatctcggt cggggagaa cttccaaga ggaaaaggt 
      tttcgggtc aaaggccga gttgcaatt gcacacgca 
      agctcatat gcgcgaagc caaaccgta caagtgttc 
      gcagtgttg taaggcgtt cgccaacag ttcctattt 
      atcgcagca cactaggat ccacttagg tatcaaacc 
      gtaccgatg cgaaatctg ccaaaggaa gttcacaca 
      actcagcca tttgcaaca acacattcg tacgcatac 
      gggcgataa accctacaa atgtcgaca tccaggttg 
      tcagaaggc gttctcgca gctgagcaa tttgcagtc 
      gcattcgag gtgtcatca aacggacaa accgtacaa 
      atgcaattc gtgctacaa gtgcttcag cgacgagcc 
      gtccctact cgaacacat tcccaaaca taaagagag 
      caagcattt aaagacgca catatgtca gtattgtgg 
      caagagcta tacgcagga gacctatct cagtaaaca 
      tatgcaaaa acacgcgga aaggacgga caagagacc 
      gccgatagg gcccgcgct aggattgaa ccaccgagg 
      cttggagca cccctactg gccgaaagt gagccccga 
      cagcgctga gaacatgca cgagtaccc ctctgacat 
      cccccggca gatcctcga gcagaacga ggacctggt 
      gatcatccg gcagcagct gcagaaccc gcccgcgcc 
      ccccggcac ccccaccgc caacctgtc gaacagcta 
      cgacgcctc gatatcgaa atccaccac caactcggc 
      cttcacccc catcaactc catgtcggg ccacctcaa 
      cctcaacca ccaccagct ggcccccaa caggcccta 
      catctacga cgccatcag cttccagaa gcagaacag 
      tctcgacct gcccaagag ccagccctc caacggctt 
      ccccaacca gctgatctc cctccacca gatccggaa 
      ctacgccca ccagcccaa cgccggcct catggagca 
      ctccatcct gggcgggct caaggacaa gtagggggc 
      ggggccccc gccgggtta tgactgtga tcggaggca 
      atagttggc tgatttaaa aaaaaagac aatttgtga 
      atactttgt acagaagac ataggttta tttcactgg 
      gccgggggc aacgccccc ggcatagct caatatatt 
      tgtaataat ataatttat ttttaattt ttttccagt 
      ttttcgtag ggaaacagc aacgcaatc gttttaaag 
      ttcaacggt actgaaact gttacgtat tttgccgaa 
      acgtggcgt tcaaatcgc gcgaaaaaa ataccccaa 
      gttaaaaac cagttaaga aaaaaaatt gtcggctat 
      tttattttt tgcgaaaaa atcgtgtat ttttttcaa 
      gcgaattgg aggttatgt ttgtccata acaaatata 
      tatttttcg tttaaataa aacttcagt atggttgaa 
      cgttgagtt gagattttg ttagggacg gttacattg 
      ttgttaaga aatgcaatt acgttatgt taacgttac 
      gttacgacc tctgttaag tttcaagtg aagaaagct 
      ttaacttac gaaattatc ccctaatta ggctttttt 
      cttttgtaa gtagcttgt gatcagaaa taatgttgt 
      atttatatt taaaaaatt atcggtttt tgttatata 
      ctttattaa taaaaatta caattagtt acttttttc 
      tttttttat tttgcagat tttttcaac tgaatgttt 
      atataactg tgataattt ttcgtactg aaaggttaa 
      taaacatta gtcaatttg taaataaaa agaaatttt 
      tatggaaag taggtaaac gcaatatga aggttccga 
      tccttaact taagcactt tagggttgg tttnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nncgacttt aataaatgt cttttctgc 
      ataagataa atgtcgtcg ttgtaaagg aaaaacact 
      aaaactctc ttttcagat tttaatgac acacaatac 
      aaacaacac aacgaattg attgttgat ttttacgtt 
      aaattttcg ttggaggtc tttagttat ttcgttttt 
      gcgcacaag aggaataac taggacttt tctcagcac 
      gtttaggag taattacat ttttcgagt tattggtgt 
      ctttgaaaa atttaatgt tatggtatg tttattgaa 
      tgtaaagaa ataaataat ttaaatcat t
    • >TC031853 RB
      gcgttgaaa tcaccacaa gtgcgttca gttcaccgg 
      aacccggaa ttcagcccg gaaccggcg gtcaagttc 
      aaaggcgtg attatctcc gtcgtcgaa gatgtatcc 
      ttggtaccg tgactccgt tggcggtca attagggca 
      attatgcgg cccgatggc caccacaat caaaaccga 
      aaacggcta caacgactg catgctggc cctcgacta 
      cgccacgca gagtaagaa agcattcgg ctgggacgg 
      ggcccaatc gggtggcag cccctcggg gccgcagtc 
      ggggggcgt gggaggcca gggggccca aggactcgt 
      ccactggat gagtgttat ggctgagca tatggatcc 
      agcgatgtc gcattacat gtcctggaa tcaagagtg 
      cggccaaca tgccaaaga cgattaccc caattggac 
      gaggaacac gatgggcat caataagca ggggtacga 
      ggccaagat gaacgaaca taatcagat gcaccagaa 
      aaacctgga cgaccacca catgggggg cacgccggg 
      cctgtacac gtcggggcg ttcgacgtc gtcgagctc 
      tagcccggg ggtggccgg ctcgagtcc ggccctgtt 
      ggtggtgcc ccagcccat caacgcgac caaaatggg 
      cggaatgca aaacggcgg cccgcaacc acggaagta 
      ccaatgtaa aatgtgccc gcagtatag tttttgcta 
      ccattcgga gcgctcgtt tttttcttt ttttttcgc 
      ggttattgt catttattt tttcaccga gcgagtgac 
      cagtgcttg taataatcg aaaaaaagt aaaaaaaaa 
      gtggtcgat ggatggccg attggagta ccgcaccgg 
      cggcctgga cttccacca gccgtcgcc gatgctcct 
      cgacaacca ccagaacta ctcctcgca cctgcagcg 
      ctccatcga cacgctgcg ctcgctggg cgaccgcct 
      gggggagcg gcccatgga cctcaacct cagcctgat 
      gcaggagcg catgaacct ggacctcaa ccacctgca 
      ggagcggcc gctcatgga caggaacaa cctggagcg 
      cggctacga gcggctgcg cgagcccca ggtggacat 
      gcggcacga ggacttgaa gtacaggga gtacaaggc 
      gcacctgga gaacctccg ctcggagag ttcaaggtc 
      gatggaagg gaggacgga cgaggaccg gggcacgac 
      gccgtcgac gccgcccac gccgatctc ggtcgggga 
      gaacttcca agaggaaaa ggttttcgg gtcaaaggc 
      cgagttgca attgcacac gcaagctca tatgcgcga 
      agccaaacc gtacaagtg ttcgcagtg ttgtaaggc 
      gttcgccaa cagttccta tttatcgca gcacactag 
      gatccactt aggtatcaa accgtaccg atgcgaaat 
      ctgccaaag gaagttcac acaactcag ccatttgca 
      acaacacat tcgtacgca tacgggcga taaacccta 
      caaatgtcg acatccagg ttgtcagaa ggcgttctc 
      gcagctgag caatttgca gtcgcattc gaggtgtca 
      tcaaacgga caaaccgta caaatgcaa ttcgtgcta 
      caagtgctt cagcgacga gccgtccct actcgaaca 
      cattcccaa acataaaga gagcaagca tttaaagac 
      gcacatatg tcagtattg tggcaagag ctatacgca 
      ggagaccta tctcagtaa acatatgca aaaacacgc 
      ggaaaggac ggacaagag accgccgat agggcccgc 
      gctaggatt gaaccaccg aggcttgga gcaccccta 
      ctggccgaa agtgagccc cgacagcgc tgagaacat 
      gcacgagta cccctctga catcccccg gcagatcct 
      cgagcagaa cgaggacct ggtgatcat ccggcagca 
      gctgcagaa cccgcccgc gccccccgg cacccccac 
      cgccaacct gtcgaacag ctacgacgc ctcgatatc 
      gaaatccac caccaactc ggccttcac ccccatcaa 
      ctccatgtc gggccacct caacctcaa ccaccacca 
      gctggcccc caacaggcc ctacatcta cgacgccat 
      cagcttcca gaagcagaa cagtctcga cctgcccaa 
      gagccagcc ctccaacgg cttccccaa ccagctgat 
      ctccctcca ccagatccg gaactacgc ccaccagcc 
      caacgccgg cctcatgga gcactccat cctgggcgg 
      gctcaagga caagtaggg ggcggggcc cccgccggg 
      ttatgactg tgatcggag gcaatagtt ggctgattt 
      aaaaaaaaa gacaatttg tgaatactt tgtacagaa 
      gacataggt ttatttcac tgggccggg ggcaacgcc 
      cccggcata gctcaatat atttgtaat aatataatt 
      tatttttaa tttttttcc agtttttcg tagggaaac 
      agcaacgca atcgtttta aagttcaac ggtactgaa 
      actgttacg tattttgcc gaaacgtgg cgttcaaat 
      cgcgcgaaa aaaataccc caagttaaa aaccagtta 
      agaaaaaaa attgtcggc tattttatt ttttgcgaa 
      aaaatcgtg tattttttt caagcgaat tggaggtta 
      tgtttgtcc ataacaaat atatatttt tcgtttaaa 
      taaaacttc agtatggtt gaacgttga gttgagatt 
      ttgttaggg acggttaca ttgttgtta agaaatgca 
      attacgtta tgttaacgt tacgttacg acctctgtt 
      aagtttcaa gtgaagaaa gctttaact tacgaaatt 
      atcccctaa ttaggcttt tttcttttg taagtagct 
      tgtgatcag aaataatgt tgtatttat atttaaaaa 
      attatcggt ttttgttat atactttat taataaaaa 
      ttacaatta gttactttt ttctttttt tattttgca 
      gattttttc aactgaatg tttatataa ctgtgataa 
      tttttcgta ctgaaaggt taataaaca ttagtcaat 
      ttgtaaata aaaagaaat ttttatgga aagtaggta 
      aacgcaata tgaaggttc cgatcctta acttaagca 
      ctttagggt tggtttnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnncgac 
      tttaataaa tgtcttttc tgcataaga taaatgtcg 
      tcgttgtaa aggaaaaac actaaaact ctcttttca 
      gattttaat gacacacaa tacaaacaa cacaacgaa 
      ttgattgtt gatttttac gttaaattt tcgttggag 
      gtctttagt tatttcgtt tttgcgcac aagaggaat 
      aactaggac ttttctcag cacgtttag gagtaatta 
      catttttcg agttattgg tgtctttga aaaatttaa 
      tgttatggt atgtttatt gaatgtaaa gaaataaat 
      aatttaaat catt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY