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Gene information
General information
TC015976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 183054..190887
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015976
    iBeetle screen iB_05935

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015976 RA
      MFRTTGNFD KLLDKATSN LLMEPDWPS ILQLCDLIR 
      QNDVQPKHA LNAVKKKLF SPNQHTAMY ALLVLESMV 
      KNCGYPLHD ELTTRPFCD TLYDLAKTT PHETVRQKL 
      FELIQAWNF AFRKSPKHG ALKDTMMMM KNDGFKFPT 
      FRESDAMFS ADSAPEWAD GDVCHRCRT AFSLIQRKH 
      HCRACGQVF CNQCSQKTT TLPKYGIEK EVRVCDACY 
      DLATKPSSG KADKQESEL PPEYVNSSL AQQSQTPPR 
      KSDEELREE EELQLALAI SQSEAEAKE KEKFKMVNT 
      YKAPVERSP SPQPEQANP ELARYLNRS YWESLNQNE 
      AAPEARAAS PSAPASATA PATEITKPK HKENGVVDN 
      DIEEFISTL KSQVEIFIN RMKSNSSRG RSIANDTSV 
      QTIFMNITA MHSKLLRYI QQHDDSRLH YERLQDKLT 
      QVKDARAAL DDLREEHRE KLRREAEEA ERQRQLQMA 
      HKLEIMRKK KQEYLQYQR QLALQRIQE QEREMQMRQ 
      EHQKQRYMM GYMGSNQFP PPPNSAPPQ SAPTYHNYQ 
      YGPMVPPQN PPPQMGIPP PQMPQPQPH FQPMYGVPP 
      IANQPMMNP MPMQAPPPN QGMPPPGFQ PPQQQQQQE 
      RKSETRTAE LISFDD
    CDS sequence
    • >TC015976 RA
      atgttccgt actacaggc aatttcgac aagcttttg 
      gacaaagct acaagcaat ttgttgatg gaaccagat 
      tggccatcg attcttcaa ctttgcgat ttaatacgt 
      cagaatgat gtacaaccc aaacatgcc ctaaatgcc 
      gtgaagaag aaattgttt tctccaaat caacacact 
      gcgatgtat gctcttttg gttcttgaa agcatggtg 
      aaaaattgc ggttatcct ttacatgac gagctcacc 
      acacgtccc ttctgcgac accctctac gacctcgcc 
      aaaaccacc cctcacgaa accgtccgc caaaagctc 
      ttcgaactg atccaagcc tggaatttc gcatttcgt 
      aaaagcccc aaacacggt gctctcaaa gacaccatg 
      atgatgatg aaaaacgat ggtttcaaa tttccgacg 
      ttccgcgaa tccgacgca atgtttagt gctgattcc 
      gctcccgaa tgggccgac ggtgatgtg tgccaccgt 
      tgcaggacc gcatttagt ttgattcag cgcaaacac 
      cattgcagg gcttgcgga caagttttt tgcaatcag 
      tgcagtcag aaaaccacc actttgcca aaatatggc 
      attgagaag gaggtgaga gtttgcgac gcgtgctat 
      gatttagca acgaaacct agtagcgga aaggctgat 
      aagcaggag agtgagttg cctccggag tatgtgaat 
      agttcgctg gctcagcag agtcaaacg cctccgagg 
      aaaagtgat gaggagttg agggaggag gaggagttg 
      cagttggct ttggcgatt agccagtca gaggctgag 
      gccaaggag aaggagaag tttaaaatg gttaacact 
      tataaagct cctgtcgaa cgcagtcca tctcctcaa 
      cccgagcaa gcaaacccc gaacttgca cgatactta 
      aatcgctct tattgggaa tctctgaac caaaatgaa 
      gcagcacca gaagcaaga gctgctagt ccatcagcc 
      ccagcttcg gccacagcc ccagccacc gaaatcacc 
      aaaccaaaa cacaaagaa aacggcgtt gttgataac 
      gacatcgaa gagttcatc agcactctc aaatcccaa 
      gtcgagatt ttcatcaat cgtatgaaa agcaactca 
      agtcgtggc cgttccatc gctaacgat accagcgtc 
      caaacaatt ttcatgaac atcacagca atgcattcg 
      aaacttcta cgctacatc caacaacac gacgacagc 
      cgattgcac tacgaacgc ctccaagac aaacttacc 
      caggtcaag gacgccaga gccgccctt gacgacctg 
      agggaagaa caccgtgag aaattgcga cgcgaggcg 
      gaagaagcc gaacgccaa cgccaactg caaatggcg 
      cataaacta gaaattatg cgaaagaaa aaacaggaa 
      tatctccaa taccaaaga cagttggcg ttgcagcgc 
      atccaggaa caagaacgt gaaatgcaa atgagacaa 
      gaacaccaa aaacagaga tatatgatg ggatatatg 
      ggcagtaac caattcccc cctccaccg aacagcgcc 
      ccgcctcaa tccgccccc acttaccac aattaccaa 
      tacggtccg atggtaccg cctcaaaac ccccctccc 
      cagatggga atcccccca cctcaaatg ccgcaacca 
      caaccacat ttccaacca atgtatggc gtcccaccc 
      atcgccaac caaccaatg atgaatcct atgccaatg 
      caagctccg cccccgaat cagggaatg ccgcctccg 
      gggttccaa cccccgcaa caacagcag caacaagag 
      cgtaaaagc gaaacacgg accgctgag ttgattagt 
      tttgatgat taa
    mRNA sequence
    • >TC015976 RA
      aagaaatgt cattgatga ttcactgtt cgttatcga 
      caaaacttt aagtgttaa taatagaat aaatacatc 
      aaattaatc gatttcgtt gttatttgt ggaaacttc 
      agtgtaaag tgtgaagat gttccgtac tacaggcaa 
      tttcgacaa gcttttgga caaagctac aagcaattt 
      gttgatgga accagattg gccatcgat tcttcaact 
      ttgcgattt aatacgtca gaatgatgt acaacccaa 
      acatgccct aaatgccgt gaagaagaa attgttttc 
      tccaaatca acacactgc gatgtatgc tcttttggt 
      tcttgaaag catggtgaa aaattgcgg ttatccttt 
      acatgacga gctcaccac acgtccctt ctgcgacac 
      cctctacga cctcgccaa aaccacccc tcacgaaac 
      cgtccgcca aaagctctt cgaactgat ccaagcctg 
      gaatttcgc atttcgtaa aagccccaa acacggtgc 
      tctcaaaga caccatgat gatgatgaa aaacgatgg 
      tttcaaatt tccgacgtt ccgcgaatc cgacgcaat 
      gtttagtgc tgattccgc tcccgaatg ggccgacgg 
      tgatgtgtg ccaccgttg caggaccgc atttagttt 
      gattcagcg caaacacca ttgcagggc ttgcggaca 
      agttttttg caatcagtg cagtcagaa aaccaccac 
      tttgccaaa atatggcat tgagaagga ggtgagagt 
      ttgcgacgc gtgctatga tttagcaac gaaacctag 
      tagcggaaa ggctgataa gcaggagag tgagttgcc 
      tccggagta tgtgaatag ttcgctggc tcagcagag 
      tcaaacgcc tccgaggaa aagtgatga ggagttgag 
      ggaggagga ggagttgca gttggcttt ggcgattag 
      ccagtcaga ggctgaggc caaggagaa ggagaagtt 
      taaaatggt taacactta taaagctcc tgtcgaacg 
      cagtccatc tcctcaacc cgagcaagc aaaccccga 
      acttgcacg atacttaaa tcgctctta ttgggaatc 
      tctgaacca aaatgaagc agcaccaga agcaagagc 
      tgctagtcc atcagcccc agcttcggc cacagcccc 
      agccaccga aatcaccaa accaaaaca caaagaaaa 
      cggcgttgt tgataacga catcgaaga gttcatcag 
      cactctcaa atcccaagt cgagatttt catcaatcg 
      tatgaaaag caactcaag tcgtggccg ttccatcgc 
      taacgatac cagcgtcca aacaatttt catgaacat 
      cacagcaat gcattcgaa acttctacg ctacatcca 
      acaacacga cgacagccg attgcacta cgaacgcct 
      ccaagacaa acttaccca ggtcaagga cgccagagc 
      cgcccttga cgacctgag ggaagaaca ccgtgagaa 
      attgcgacg cgaggcgga agaagccga acgccaacg 
      ccaactgca aatggcgca taaactaga aattatgcg 
      aaagaaaaa acaggaata tctccaata ccaaagaca 
      gttggcgtt gcagcgcat ccaggaaca agaacgtga 
      aatgcaaat gagacaaga acaccaaaa acagagata 
      tatgatggg atatatggg cagtaacca attcccccc 
      tccaccgaa cagcgcccc gcctcaatc cgcccccac 
      ttaccacaa ttaccaata cggtccgat ggtaccgcc 
      tcaaaaccc ccctcccca gatgggaat ccccccacc 
      tcaaatgcc gcaaccaca accacattt ccaaccaat 
      gtatggcgt cccacccat cgccaacca accaatgat 
      gaatcctat gccaatgca agctccgcc cccgaatca 
      gggaatgcc gcctccggg gttccaacc cccgcaaca 
      acagcagca acaagagcg taaaagcga aacacggac 
      cgctgagtt gattagttt tgatgatta aatttgtat 
      ggggttatt tttttttca ataaatgta tggctacag 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY