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Gene information
General information
TC015975 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 196666..200797
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015975
    iBeetle screen iB_05934

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased # of remaining segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015975 RA
      MVVEKRRAE DSSSLVPVS KKSKNEVAT SNKNKSVLQ 
      AAPARTSNL FAPIMLLEG HEGEIFTVE FHPEGQYVA 
      SSGFDRRIF VWSVYGECE NLSVMSGHT GAVMELHFT 
      TDGTNIFTA STDHTLGLW DLPTSQRIK KYKGHTTFV 
      NSVQGARRG PQMLVSGSD DTTIKLWDI RKKQSVTTF 
      NSNYQVTAV EFNDTAEQI FSGGIDNDI KVWDIRNHE 
      IIYTLKGHT DTVTGLALS PDGSYLLSN SMDNSLRIW 
      DVRPYAPQE RCVKVFTGH QHNFEKNLL RCAWSKDGS 
      KVSSGSADR FLYIWDTTS RRIIYKLPG HNGSVNDVD 
      FHPNEPIVV SGASDKQIY LGEID
    CDS sequence
    • >TC015975 RA
      atggttgtt gagaaacgt cgagccgaa gattcatca 
      tctctagtc cccgtaagc aaaaaatcg aaaaatgaa 
      gtggccaca tcgaacaaa aacaaatcg gtcttacaa 
      gcggcccct gcgagaact tccaatcta tttgccccc 
      attatgtta ctggagggc cacgagggt gagattttc 
      acagtggaa ttccaccct gagggtcaa tatgtagct 
      tcatcaggt ttcgaccgt agaattttc gtttggagc 
      gtttacggt gagtgtgaa aatttatcg gtaatgagt 
      ggccacacg ggtgctgtc atggagctc cacttcacg 
      acagatggc actaacatt ttcacagcc agcactgat 
      cacacgctt ggcctttgg gatttacca acaagtcaa 
      agaattaaa aaatacaaa ggtcacact acttttgtt 
      aattcggtc caaggtgcg agacgaggc cctcaaatg 
      ttggtctca ggcagcgat gacaccacg attaaatta 
      tgggatatt cgcaaaaaa caatccgtc acaactttc 
      aactcaaat tatcaagtc actgctgtt gaatttaac 
      gacacagct gaacagatt ttcagcgga gggattgat 
      aatgatata aaagtgtgg gatattcgc aaccatgaa 
      attatttat acacttaag gggcacacg gatacggtg 
      acagggttg gctttgagc ccagacggt tcgtattta 
      ttatcaaat tcgatggat aatagtctg aggatatgg 
      gacgtaagg ccatatgcg ccccaagaa aggtgtgtt 
      aaggttttt acgggacat cagcataat ttcgagaag 
      aatcttctg cggtgtgct tggtccaaa gatgggtct 
      aaggttagt tcaggatca gctgatagg tttctctac 
      atttgggat accacctct cggaggatt atttataag 
      ttgcctggg cataacggt agtgttaac gatgttgat 
      ttccatcca aatgagcca attgtggtc tcgggtgca 
      agtgacaag cagatttat ttaggtgag atagattaa
    mRNA sequence
    • >TC015975 RA
      ggcgcttat cgcttcgtt ttgttgacg ctaaaaagt 
      gaggttatt gttttcctc ccaattttt gtttaaaac 
      accaaaaat ggttgttga gaaacgtcg agccgaaga 
      ttcatcatc tctagtccc cgtaagcaa aaaatcgaa 
      aaatgaagt ggccacatc gaacaaaaa caaatcggt 
      cttacaagc ggcccctgc gagaacttc caatctatt 
      tgcccccat tatgttact ggagggcca cgagggtga 
      gattttcac agtggaatt ccaccctga gggtcaata 
      tgtagcttc atcaggttt cgaccgtag aattttcgt 
      ttggagcgt ttacggtga gtgtgaaaa tttatcggt 
      aatgagtgg ccacacggg tgctgtcat ggagctcca 
      cttcacgac agatggcac taacatttt cacagccag 
      cactgatca cacgcttgg cctttggga tttaccaac 
      aagtcaaag aattaaaaa atacaaagg tcacactac 
      ttttgttaa ttcggtcca aggtgcgag acgaggccc 
      tcaaatgtt ggtctcagg cagcgatga caccacgat 
      taaattatg ggatattcg caaaaaaca atccgtcac 
      aactttcaa ctcaaatta tcaagtcac tgctgttga 
      atttaacga cacagctga acagatttt cagcggagg 
      gattgataa tgatataaa agtgtggga tattcgcaa 
      ccatgaaat tatttatac acttaaggg gcacacgga 
      tacggtgac agggttggc tttgagccc agacggttc 
      gtatttatt atcaaattc gatggataa tagtctgag 
      gatatggga cgtaaggcc atatgcgcc ccaagaaag 
      gtgtgttaa ggtttttac gggacatca gcataattt 
      cgagaagaa tcttctgcg gtgtgcttg gtccaaaga 
      tgggtctaa ggttagttc aggatcagc tgataggtt 
      tctctacat ttgggatac cacctctcg gaggattat 
      ttataagtt gcctgggca taacggtag tgttaacga 
      tgttgattt ccatccaaa tgagccaat tgtggtctc 
      gggtgcaag tgacaagca gatttattt aggtgagat 
      agattaaaa aaatgtgaa attttataa taaaagtat 
      taatgaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY