Status
Please wait ...
Gene information
General information
TC015972 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 272179..302332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015972
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015972 RA
      MPLADIIRR ASTYILGTD EELSKMSQY EDNEVLFCK 
      NNVCVHPPA MVRQDTDIL HHPGYLTIT SKVFVDQYN 
      ETKRPTLFL TWIPNTALT KCPSALQNS YNHNKQTSM 
      ESLNSNDSN GSSIPERPA SIELKSTNP FLNYDTTDS 
      LSSEDSDKQ GTIININVD IANPDIEIV TTPDSVKDS 
      GEKFEFSRS GSVTSNDSQ LNWITTPEF LAQKHNLMF 
      PESNNCSPI LPTKQLHKC RRFSVDLSQ MRSLRLFFN 
      DANCTCGQL VVASRESQY KILHFHHGG LDHLAQVLH 
      QWHSLLHNI KHAKGYEEN LPYRHFMVC RPEVSDLEL 
      HPEEGQVPK LTEEMFRSL FNEIGQLED DLSLRKSIF 
      FSGMERNLR KEVWPFLLH VYPYQSTYT ERSQIAEIR 
      RQEYDEITR RRLDLSGVQ LNQFRRKVQ SVIEKDVVR 
      TDRGNPFFA GDDNPNLDV MKNILLNYA VYNPGLGYT 
      QGMSDLLAP VLCELRDEV AAFWCFVGL MQRAVFVAT 
      PTDRDMDRS LRFLRELVK LMVPRFYEH LEKHKDATE 
      LLFCHRWIL LCFKREFTE AVALRMWEA CWANYLTDY 
      FHLFLCLAI ICVYADDVI AQDLRADEM LLHFSSLAM 
      YMDGQVIAR KARGLLHQF RQLREIPCT LSGLCTRCG 
      PGIWDSSHS PRIYCTGHD LPGHCNNCI N
    CDS sequence
    • >TC015972 RA
      atgcctctg gcagatata attcggcgc gcgtcgacg 
      tacatttta ggcaccgat gaagagtta tcgaagatg 
      tctcagtac gaagacaac gaggtgctc ttctgcaaa 
      aacaacgtg tgtgtgcat cctccagcg atggtacgg 
      caagacact gatattctc catcatccc ggatatctc 
      acaatcacc tccaaagtc tttgttgat caatacaac 
      gagacgaaa agacccacg cttttcctc acctggatc 
      ccgaataca gctctaacc aaatgtccg agtgcttta 
      caaaattca tacaaccac aataagcag accagcatg 
      gagagcctg aactcgaat gattcaaac gggagcagc 
      attccagaa cgtccagca agtatcgaa ctcaaaagc 
      acgaacccg ttcctaaac tacgacacc accgattcc 
      ctcagttcc gaagacagt gacaagcaa ggcactatc 
      atcaacatc aacgtcgac atcgcaaat cccgacata 
      gaaatcgtt acaactcca gacagcgtc aaagattcc 
      ggcgaaaaa ttcgaattt tcccgctcc ggcagtgtt 
      acttcaaac gacagccaa ttgaattgg atcacaaca 
      ccggaattt ctcgcccaa aagcataat ttgatgttc 
      cccgaaagc aacaactgt tcgccaatt ttaccaaca 
      aaacagcta cataaatgc aggaggttc agtgttgat 
      ttgagccaa atgaggtca ctgagactg tttttcaac 
      gatgcaaat tgcacgtgt ggacaactt gttgtggct 
      tcgagggaa tcgcaatac aaaattttg cacttccat 
      cacggaggg ttggaccac ttggcgcaa gtgttgcat 
      cagtggcat tctttgctg cataatatc aaacatgcc 
      aaaggttat gaagaaaac cttccctac cgtcacttt 
      atggtatgt cggcccgaa gtgtccgac cttgagctc 
      cacccagaa gagggccaa gtccccaaa ctcaccgaa 
      gaaatgttc cgaagtctg ttcaacgaa atcggtcaa 
      ctggaagac gacttaagc ttacgcaaa tcaattttt 
      ttctccgga atggagcga aaccttcga aaggaagtc 
      tggcccttc ctcctccac gtctacccc taccaaagc 
      acttacacc gagcgaagc caaattgca gaaatccgc 
      cgtcaagaa tacgacgaa atcacaaga cgaagactg 
      gacttatct ggagtgcaa ctcaaccaa ttccgacga 
      aaagtccaa agcgtgatt gagaaggac gtcgtgcga 
      accgaccga ggaaaccct tttttcgcc ggcgatgac 
      aatccaaat ttagacgtg atgaagaat attttacta 
      aactatgcc gtgtataac ccaggtttg gggtacacc 
      caagggatg agtgatctg ctggcgcct gttctttgc 
      gagttgaga gatgaagtg gcagcgttt tggtgcttc 
      gttgggctg atgcaaagg gcggttttc gtcgcgaca 
      cccacggat agggatatg gacaggtcg ttgaggttc 
      ttgagggag ttggtcaag ttgatggtg ccgagattt 
      tacgaacat ttggaaaag cacaaggat gcgaccgag 
      ctgctcttc tgccatcgc tggattttg ttgtgtttt 
      aagcgcgaa tttaccgaa gctgtggcc ttgcgaatg 
      tgggaggcc tgttgggca aactatttg acggactat 
      ttccacttg ttcttgtgt cttgctata atctgtgtt 
      tatgccgac gatgtcatt gctcaagat ttaagagct 
      gatgaaatg ttgctgcat tttagttcg ctcgccatg 
      tatatggac ggacaagtc atcgcacgt aaagctaga 
      ggtctgctt catcagttt aggcaattg agggaaatt 
      ccctgtact ttgtcgggc ctttgtacg agatgtggg 
      cctggaatt tgggatagt tctcacagt ccgaggatt 
      tattgtaca gggcacgac cttccagga cattgtaat 
      aactgcatc aactaa
    mRNA sequence
    • >TC015972 RA
      tatatttct acatttgtg ataatttcg ttcaataag 
      cgcgcactt tgctgacag gcacgtaga tatttggat 
      caataaaaa cagtagggc atgagggcg gcataacgc 
      tcaacatgg tgccaacgg gtcaaatat ttcttcaca 
      aaaggaaag attcgaatt aaatattga ctcaagtgt 
      gaaaaaatt ataaaataa tccctagtg aagatgtgt 
      agttagtgt tataaatga ttgaaatca tgcctctgg 
      cagatataa ttcggcgcg cgtcgacgt acattttag 
      gcaccgatg aagagttat cgaagatgt ctcagtacg 
      aagacaacg aggtgctct tctgcaaaa acaacgtgt 
      gtgtgcatc ctccagcga tggtacggc aagacactg 
      atattctcc atcatcccg gatatctca caatcacct 
      ccaaagtct ttgttgatc aatacaacg agacgaaaa 
      gacccacgc ttttcctca cctggatcc cgaatacag 
      ctctaacca aatgtccga gtgctttac aaaattcat 
      acaaccaca ataagcaga ccagcatgg agagcctga 
      actcgaatg attcaaacg ggagcagca ttccagaac 
      gtccagcaa gtatcgaac tcaaaagca cgaacccgt 
      tcctaaact acgacacca ccgattccc tcagttccg 
      aagacagtg acaagcaag gcactatca tcaacatca 
      acgtcgaca tcgcaaatc ccgacatag aaatcgtta 
      caactccag acagcgtca aagattccg gcgaaaaat 
      tcgaatttt cccgctccg gcagtgtta cttcaaacg 
      acagccaat tgaattgga tcacaacac cggaatttc 
      tcgcccaaa agcataatt tgatgttcc ccgaaagca 
      acaactgtt cgccaattt taccaacaa aacagctac 
      ataaatgca ggaggttca gtgttgatt tgagccaaa 
      tgaggtcac tgagactgt ttttcaacg atgcaaatt 
      gcacgtgtg gacaacttg ttgtggctt cgagggaat 
      cgcaataca aaattttgc acttccatc acggagggt 
      tggaccact tggcgcaag tgttgcatc agtggcatt 
      ctttgctgc ataatatca aacatgcca aaggttatg 
      aagaaaacc ttccctacc gtcacttta tggtatgtc 
      ggcccgaag tgtccgacc ttgagctcc acccagaag 
      agggccaag tccccaaac tcaccgaag aaatgttcc 
      gaagtctgt tcaacgaaa tcggtcaac tggaagacg 
      acttaagct tacgcaaat caatttttt tctccggaa 
      tggagcgaa accttcgaa aggaagtct ggcccttcc 
      tcctccacg tctacccct accaaagca cttacaccg 
      agcgaagcc aaattgcag aaatccgcc gtcaagaat 
      acgacgaaa tcacaagac gaagactgg acttatctg 
      gagtgcaac tcaaccaat tccgacgaa aagtccaaa 
      gcgtgattg agaaggacg tcgtgcgaa ccgaccgag 
      gaaaccctt ttttcgccg gcgatgaca atccaaatt 
      tagacgtga tgaagaata ttttactaa actatgccg 
      tgtataacc caggtttgg ggtacaccc aagggatga 
      gtgatctgc tggcgcctg ttctttgcg agttgagag 
      atgaagtgg cagcgtttt ggtgcttcg ttgggctga 
      tgcaaaggg cggttttcg tcgcgacac ccacggata 
      gggatatgg acaggtcgt tgaggttct tgagggagt 
      tggtcaagt tgatggtgc cgagatttt acgaacatt 
      tggaaaagc acaaggatg cgaccgagc tgctcttct 
      gccatcgct ggattttgt tgtgtttta agcgcgaat 
      ttaccgaag ctgtggcct tgcgaatgt gggaggcct 
      gttgggcaa actatttga cggactatt tccacttgt 
      tcttgtgtc ttgctataa tctgtgttt atgccgacg 
      atgtcattg ctcaagatt taagagctg atgaaatgt 
      tgctgcatt ttagttcgc tcgccatgt atatggacg 
      gacaagtca tcgcacgta aagctagag gtctgcttc 
      atcagttta ggcaattga gggaaattc cctgtactt 
      tgtcgggcc tttgtacga gatgtgggc ctggaattt 
      gggatagtt ctcacagtc cgaggattt attgtacag 
      ggcacgacc ttccaggac attgtaata actgcatca 
      actaaacta tgacttttg tttgagact caataggta 
      tggaaaaaa gcggtgtct aaatacgtt tatatttct 
      ctctgtgac aagaatata taattttat aaaaaatgt 
      acatactgt tcacatttt tatgatttg aatttgatt 
      gataatcgt catatcagg ttgcgtaca actgtataa 
      taattacat tgttaatca atttgcaaa cctgtattt 
      attattaga aataaatgc ttggcaagt gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY