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Gene information
General information
TC015972 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 272179..302332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015972
    iBeetle screen iB_05933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015972 RA
      MPLADIIRR ASTYILGTD EELSKMSQY EDNEVLFCK 
NNVCVHPPA MVRQDTDIL HHPGYLTIT SKVFVDQYN 
ETKRPTLFL TWIPNTALT KCPSALQNS YNHNKQTSM 
ESLNSNDSN GSSIPERPA SIELKSTNP FLNYDTTDS 
LSSEDSDKQ GTIININVD IANPDIEIV TTPDSVKDS 
GEKFEFSRS GSVTSNDSQ LNWITTPEF LAQKHNLMF 
PESNNCSPI LPTKQLHKC RRFSVDLSQ MRSLRLFFN 
DANCTCGQL VVASRESQY KILHFHHGG LDHLAQVLH 
QWHSLLHNI KHAKGYEEN LPYRHFMVC RPEVSDLEL 
HPEEGQVPK LTEEMFRSL FNEIGQLED DLSLRKSIF 
FSGMERNLR KEVWPFLLH VYPYQSTYT ERSQIAEIR 
RQEYDEITR RRLDLSGVQ LNQFRRKVQ SVIEKDVVR 
TDRGNPFFA GDDNPNLDV MKNILLNYA VYNPGLGYT 
QGMSDLLAP VLCELRDEV AAFWCFVGL MQRAVFVAT 
PTDRDMDRS LRFLRELVK LMVPRFYEH LEKHKDATE 
LLFCHRWIL LCFKREFTE AVALRMWEA CWANYLTDY 
FHLFLCLAI ICVYADDVI AQDLRADEM LLHFSSLAM 
YMDGQVIAR KARGLLHQF RQLREIPCT LSGLCTRCG 
PGIWDSSHS PRIYCTGHD LPGHCNNCI N
    CDS sequence
    • >TC015972 RA
      atgcctctg gcagatata attcggcgc gcgtcgacg 
tacatttta ggcaccgat gaagagtta tcgaagatg 
tctcagtac gaagacaac gaggtgctc ttctgcaaa 
aacaacgtg tgtgtgcat cctccagcg atggtacgg 
caagacact gatattctc catcatccc ggatatctc 
acaatcacc tccaaagtc tttgttgat caatacaac 
gagacgaaa agacccacg cttttcctc acctggatc 
ccgaataca gctctaacc aaatgtccg agtgcttta 
caaaattca tacaaccac aataagcag accagcatg 
gagagcctg aactcgaat gattcaaac gggagcagc 
attccagaa cgtccagca agtatcgaa ctcaaaagc 
acgaacccg ttcctaaac tacgacacc accgattcc 
ctcagttcc gaagacagt gacaagcaa ggcactatc 
atcaacatc aacgtcgac atcgcaaat cccgacata 
gaaatcgtt acaactcca gacagcgtc aaagattcc 
ggcgaaaaa ttcgaattt tcccgctcc ggcagtgtt 
acttcaaac gacagccaa ttgaattgg atcacaaca 
ccggaattt ctcgcccaa aagcataat ttgatgttc 
cccgaaagc aacaactgt tcgccaatt ttaccaaca 
aaacagcta cataaatgc aggaggttc agtgttgat 
ttgagccaa atgaggtca ctgagactg tttttcaac 
gatgcaaat tgcacgtgt ggacaactt gttgtggct 
tcgagggaa tcgcaatac aaaattttg cacttccat 
cacggaggg ttggaccac ttggcgcaa gtgttgcat 
cagtggcat tctttgctg cataatatc aaacatgcc 
aaaggttat gaagaaaac cttccctac cgtcacttt 
atggtatgt cggcccgaa gtgtccgac cttgagctc 
cacccagaa gagggccaa gtccccaaa ctcaccgaa 
gaaatgttc cgaagtctg ttcaacgaa atcggtcaa 
ctggaagac gacttaagc ttacgcaaa tcaattttt 
ttctccgga atggagcga aaccttcga aaggaagtc 
tggcccttc ctcctccac gtctacccc taccaaagc 
acttacacc gagcgaagc caaattgca gaaatccgc 
cgtcaagaa tacgacgaa atcacaaga cgaagactg 
gacttatct ggagtgcaa ctcaaccaa ttccgacga 
aaagtccaa agcgtgatt gagaaggac gtcgtgcga 
accgaccga ggaaaccct tttttcgcc ggcgatgac 
aatccaaat ttagacgtg atgaagaat attttacta 
aactatgcc gtgtataac ccaggtttg gggtacacc 
caagggatg agtgatctg ctggcgcct gttctttgc 
gagttgaga gatgaagtg gcagcgttt tggtgcttc 
gttgggctg atgcaaagg gcggttttc gtcgcgaca 
cccacggat agggatatg gacaggtcg ttgaggttc 
ttgagggag ttggtcaag ttgatggtg ccgagattt 
tacgaacat ttggaaaag cacaaggat gcgaccgag 
ctgctcttc tgccatcgc tggattttg ttgtgtttt 
aagcgcgaa tttaccgaa gctgtggcc ttgcgaatg 
tgggaggcc tgttgggca aactatttg acggactat 
ttccacttg ttcttgtgt cttgctata atctgtgtt 
tatgccgac gatgtcatt gctcaagat ttaagagct 
gatgaaatg ttgctgcat tttagttcg ctcgccatg 
tatatggac ggacaagtc atcgcacgt aaagctaga 
ggtctgctt catcagttt aggcaattg agggaaatt 
ccctgtact ttgtcgggc ctttgtacg agatgtggg 
cctggaatt tgggatagt tctcacagt ccgaggatt 
tattgtaca gggcacgac cttccagga cattgtaat 
aactgcatc aactaa
    mRNA sequence
    • >TC015972 RA
      tatatttct acatttgtg ataatttcg ttcaataag 
cgcgcactt tgctgacag gcacgtaga tatttggat 
caataaaaa cagtagggc atgagggcg gcataacgc 
tcaacatgg tgccaacgg gtcaaatat ttcttcaca 
aaaggaaag attcgaatt aaatattga ctcaagtgt 
gaaaaaatt ataaaataa tccctagtg aagatgtgt 
agttagtgt tataaatga ttgaaatca tgcctctgg 
cagatataa ttcggcgcg cgtcgacgt acattttag 
gcaccgatg aagagttat cgaagatgt ctcagtacg 
aagacaacg aggtgctct tctgcaaaa acaacgtgt 
gtgtgcatc ctccagcga tggtacggc aagacactg 
atattctcc atcatcccg gatatctca caatcacct 
ccaaagtct ttgttgatc aatacaacg agacgaaaa 
gacccacgc ttttcctca cctggatcc cgaatacag 
ctctaacca aatgtccga gtgctttac aaaattcat 
acaaccaca ataagcaga ccagcatgg agagcctga 
actcgaatg attcaaacg ggagcagca ttccagaac 
gtccagcaa gtatcgaac tcaaaagca cgaacccgt 
tcctaaact acgacacca ccgattccc tcagttccg 
aagacagtg acaagcaag gcactatca tcaacatca 
acgtcgaca tcgcaaatc ccgacatag aaatcgtta 
caactccag acagcgtca aagattccg gcgaaaaat 
tcgaatttt cccgctccg gcagtgtta cttcaaacg 
acagccaat tgaattgga tcacaacac cggaatttc 
tcgcccaaa agcataatt tgatgttcc ccgaaagca 
acaactgtt cgccaattt taccaacaa aacagctac 
ataaatgca ggaggttca gtgttgatt tgagccaaa 
tgaggtcac tgagactgt ttttcaacg atgcaaatt 
gcacgtgtg gacaacttg ttgtggctt cgagggaat 
cgcaataca aaattttgc acttccatc acggagggt 
tggaccact tggcgcaag tgttgcatc agtggcatt 
ctttgctgc ataatatca aacatgcca aaggttatg 
aagaaaacc ttccctacc gtcacttta tggtatgtc 
ggcccgaag tgtccgacc ttgagctcc acccagaag 
agggccaag tccccaaac tcaccgaag aaatgttcc 
gaagtctgt tcaacgaaa tcggtcaac tggaagacg 
acttaagct tacgcaaat caatttttt tctccggaa 
tggagcgaa accttcgaa aggaagtct ggcccttcc 
tcctccacg tctacccct accaaagca cttacaccg 
agcgaagcc aaattgcag aaatccgcc gtcaagaat 
acgacgaaa tcacaagac gaagactgg acttatctg 
gagtgcaac tcaaccaat tccgacgaa aagtccaaa 
gcgtgattg agaaggacg tcgtgcgaa ccgaccgag 
gaaaccctt ttttcgccg gcgatgaca atccaaatt 
tagacgtga tgaagaata ttttactaa actatgccg 
tgtataacc caggtttgg ggtacaccc aagggatga 
gtgatctgc tggcgcctg ttctttgcg agttgagag 
atgaagtgg cagcgtttt ggtgcttcg ttgggctga 
tgcaaaggg cggttttcg tcgcgacac ccacggata 
gggatatgg acaggtcgt tgaggttct tgagggagt 
tggtcaagt tgatggtgc cgagatttt acgaacatt 
tggaaaagc acaaggatg cgaccgagc tgctcttct 
gccatcgct ggattttgt tgtgtttta agcgcgaat 
ttaccgaag ctgtggcct tgcgaatgt gggaggcct 
gttgggcaa actatttga cggactatt tccacttgt 
tcttgtgtc ttgctataa tctgtgttt atgccgacg 
atgtcattg ctcaagatt taagagctg atgaaatgt 
tgctgcatt ttagttcgc tcgccatgt atatggacg 
gacaagtca tcgcacgta aagctagag gtctgcttc 
atcagttta ggcaattga gggaaattc cctgtactt 
tgtcgggcc tttgtacga gatgtgggc ctggaattt 
gggatagtt ctcacagtc cgaggattt attgtacag 
ggcacgacc ttccaggac attgtaata actgcatca 
actaaacta tgacttttg tttgagact caataggta 
tggaaaaaa gcggtgtct aaatacgtt tatatttct 
ctctgtgac aagaatata taattttat aaaaaatgt 
acatactgt tcacatttt tatgatttg aatttgatt 
gataatcgt catatcagg ttgcgtaca actgtataa 
taattacat tgttaatca atttgcaaa cctgtattt 
attattaga aataaatgc ttggcaagt gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY