| General information | |||
| TC015972 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 272179..302332 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015972 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05933 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015972 RA
MPLADIIRR ASTYILGTD EELSKMSQY EDNEVLFCK NNVCVHPPA MVRQDTDIL HHPGYLTIT SKVFVDQYN ETKRPTLFL TWIPNTALT KCPSALQNS YNHNKQTSM ESLNSNDSN GSSIPERPA SIELKSTNP FLNYDTTDS LSSEDSDKQ GTIININVD IANPDIEIV TTPDSVKDS GEKFEFSRS GSVTSNDSQ LNWITTPEF LAQKHNLMF PESNNCSPI LPTKQLHKC RRFSVDLSQ MRSLRLFFN DANCTCGQL VVASRESQY KILHFHHGG LDHLAQVLH QWHSLLHNI KHAKGYEEN LPYRHFMVC RPEVSDLEL HPEEGQVPK LTEEMFRSL FNEIGQLED DLSLRKSIF FSGMERNLR KEVWPFLLH VYPYQSTYT ERSQIAEIR RQEYDEITR RRLDLSGVQ LNQFRRKVQ SVIEKDVVR TDRGNPFFA GDDNPNLDV MKNILLNYA VYNPGLGYT QGMSDLLAP VLCELRDEV AAFWCFVGL MQRAVFVAT PTDRDMDRS LRFLRELVK LMVPRFYEH LEKHKDATE LLFCHRWIL LCFKREFTE AVALRMWEA CWANYLTDY FHLFLCLAI ICVYADDVI AQDLRADEM LLHFSSLAM YMDGQVIAR KARGLLHQF RQLREIPCT LSGLCTRCG PGIWDSSHS PRIYCTGHD LPGHCNNCI N
- >TC015972 RA
atgcctctg gcagatata attcggcgc gcgtcgacg tacatttta ggcaccgat gaagagtta tcgaagatg tctcagtac gaagacaac gaggtgctc ttctgcaaa aacaacgtg tgtgtgcat cctccagcg atggtacgg caagacact gatattctc catcatccc ggatatctc acaatcacc tccaaagtc tttgttgat caatacaac gagacgaaa agacccacg cttttcctc acctggatc ccgaataca gctctaacc aaatgtccg agtgcttta caaaattca tacaaccac aataagcag accagcatg gagagcctg aactcgaat gattcaaac gggagcagc attccagaa cgtccagca agtatcgaa ctcaaaagc acgaacccg ttcctaaac tacgacacc accgattcc ctcagttcc gaagacagt gacaagcaa ggcactatc atcaacatc aacgtcgac atcgcaaat cccgacata gaaatcgtt acaactcca gacagcgtc aaagattcc ggcgaaaaa ttcgaattt tcccgctcc ggcagtgtt acttcaaac gacagccaa ttgaattgg atcacaaca ccggaattt ctcgcccaa aagcataat ttgatgttc cccgaaagc aacaactgt tcgccaatt ttaccaaca aaacagcta cataaatgc aggaggttc agtgttgat ttgagccaa atgaggtca ctgagactg tttttcaac gatgcaaat tgcacgtgt ggacaactt gttgtggct tcgagggaa tcgcaatac aaaattttg cacttccat cacggaggg ttggaccac ttggcgcaa gtgttgcat cagtggcat tctttgctg cataatatc aaacatgcc aaaggttat gaagaaaac cttccctac cgtcacttt atggtatgt cggcccgaa gtgtccgac cttgagctc cacccagaa gagggccaa gtccccaaa ctcaccgaa gaaatgttc cgaagtctg ttcaacgaa atcggtcaa ctggaagac gacttaagc ttacgcaaa tcaattttt ttctccgga atggagcga aaccttcga aaggaagtc tggcccttc ctcctccac gtctacccc taccaaagc acttacacc gagcgaagc caaattgca gaaatccgc cgtcaagaa tacgacgaa atcacaaga cgaagactg gacttatct ggagtgcaa ctcaaccaa ttccgacga aaagtccaa agcgtgatt gagaaggac gtcgtgcga accgaccga ggaaaccct tttttcgcc ggcgatgac aatccaaat ttagacgtg atgaagaat attttacta aactatgcc gtgtataac ccaggtttg gggtacacc caagggatg agtgatctg ctggcgcct gttctttgc gagttgaga gatgaagtg gcagcgttt tggtgcttc gttgggctg atgcaaagg gcggttttc gtcgcgaca cccacggat agggatatg gacaggtcg ttgaggttc ttgagggag ttggtcaag ttgatggtg ccgagattt tacgaacat ttggaaaag cacaaggat gcgaccgag ctgctcttc tgccatcgc tggattttg ttgtgtttt aagcgcgaa tttaccgaa gctgtggcc ttgcgaatg tgggaggcc tgttgggca aactatttg acggactat ttccacttg ttcttgtgt cttgctata atctgtgtt tatgccgac gatgtcatt gctcaagat ttaagagct gatgaaatg ttgctgcat tttagttcg ctcgccatg tatatggac ggacaagtc atcgcacgt aaagctaga ggtctgctt catcagttt aggcaattg agggaaatt ccctgtact ttgtcgggc ctttgtacg agatgtggg cctggaatt tgggatagt tctcacagt ccgaggatt tattgtaca gggcacgac cttccagga cattgtaat aactgcatc aactaa
- >TC015972 RA
tatatttct acatttgtg ataatttcg ttcaataag cgcgcactt tgctgacag gcacgtaga tatttggat caataaaaa cagtagggc atgagggcg gcataacgc tcaacatgg tgccaacgg gtcaaatat ttcttcaca aaaggaaag attcgaatt aaatattga ctcaagtgt gaaaaaatt ataaaataa tccctagtg aagatgtgt agttagtgt tataaatga ttgaaatca tgcctctgg cagatataa ttcggcgcg cgtcgacgt acattttag gcaccgatg aagagttat cgaagatgt ctcagtacg aagacaacg aggtgctct tctgcaaaa acaacgtgt gtgtgcatc ctccagcga tggtacggc aagacactg atattctcc atcatcccg gatatctca caatcacct ccaaagtct ttgttgatc aatacaacg agacgaaaa gacccacgc ttttcctca cctggatcc cgaatacag ctctaacca aatgtccga gtgctttac aaaattcat acaaccaca ataagcaga ccagcatgg agagcctga actcgaatg attcaaacg ggagcagca ttccagaac gtccagcaa gtatcgaac tcaaaagca cgaacccgt tcctaaact acgacacca ccgattccc tcagttccg aagacagtg acaagcaag gcactatca tcaacatca acgtcgaca tcgcaaatc ccgacatag aaatcgtta caactccag acagcgtca aagattccg gcgaaaaat tcgaatttt cccgctccg gcagtgtta cttcaaacg acagccaat tgaattgga tcacaacac cggaatttc tcgcccaaa agcataatt tgatgttcc ccgaaagca acaactgtt cgccaattt taccaacaa aacagctac ataaatgca ggaggttca gtgttgatt tgagccaaa tgaggtcac tgagactgt ttttcaacg atgcaaatt gcacgtgtg gacaacttg ttgtggctt cgagggaat cgcaataca aaattttgc acttccatc acggagggt tggaccact tggcgcaag tgttgcatc agtggcatt ctttgctgc ataatatca aacatgcca aaggttatg aagaaaacc ttccctacc gtcacttta tggtatgtc ggcccgaag tgtccgacc ttgagctcc acccagaag agggccaag tccccaaac tcaccgaag aaatgttcc gaagtctgt tcaacgaaa tcggtcaac tggaagacg acttaagct tacgcaaat caatttttt tctccggaa tggagcgaa accttcgaa aggaagtct ggcccttcc tcctccacg tctacccct accaaagca cttacaccg agcgaagcc aaattgcag aaatccgcc gtcaagaat acgacgaaa tcacaagac gaagactgg acttatctg gagtgcaac tcaaccaat tccgacgaa aagtccaaa gcgtgattg agaaggacg tcgtgcgaa ccgaccgag gaaaccctt ttttcgccg gcgatgaca atccaaatt tagacgtga tgaagaata ttttactaa actatgccg tgtataacc caggtttgg ggtacaccc aagggatga gtgatctgc tggcgcctg ttctttgcg agttgagag atgaagtgg cagcgtttt ggtgcttcg ttgggctga tgcaaaggg cggttttcg tcgcgacac ccacggata gggatatgg acaggtcgt tgaggttct tgagggagt tggtcaagt tgatggtgc cgagatttt acgaacatt tggaaaagc acaaggatg cgaccgagc tgctcttct gccatcgct ggattttgt tgtgtttta agcgcgaat ttaccgaag ctgtggcct tgcgaatgt gggaggcct gttgggcaa actatttga cggactatt tccacttgt tcttgtgtc ttgctataa tctgtgttt atgccgacg atgtcattg ctcaagatt taagagctg atgaaatgt tgctgcatt ttagttcgc tcgccatgt atatggacg gacaagtca tcgcacgta aagctagag gtctgcttc atcagttta ggcaattga gggaaattc cctgtactt tgtcgggcc tttgtacga gatgtgggc ctggaattt gggatagtt ctcacagtc cgaggattt attgtacag ggcacgacc ttccaggac attgtaata actgcatca actaaacta tgacttttg tttgagact caataggta tggaaaaaa gcggtgtct aaatacgtt tatatttct ctctgtgac aagaatata taattttat aaaaaatgt acatactgt tcacatttt tatgatttg aatttgatt gataatcgt catatcagg ttgcgtaca actgtataa taattacat tgttaatca atttgcaaa cctgtattt attattaga aataaatgc ttggcaagt gt
