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Gene information
General information
TC015958 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3939543..3940495
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC015958
      iBeetle screen iB_05932

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015958 RA
        MDAVPEIKG ISSELLEKL EKLQKQDLF 
SFLPDVETD LKLPPLPQD LKLQFDLLD 
SLPTLKIGR KSKTIMQNM AEIEKLEQN 
YRINRDLLK YEKEERLKV QEEMCPEST 
FLALCCLHE KKGKKERQK SPLKPKDFI 
HRNIELATA MAKLPLTDE EKHQLDQLL 
ANLDDCGDQ SNGFQMCES DKQRLAEID 
TELEDKYPE SRHLRSPSK NALLDEALK 
EIDGKIATV RDE
      • >TC015958 RB
        MDAVPEIKG ISSELLEKL EKLQKQDLF SFLPDVETD 
LKLPPLPQG LPNFNSAFF NYSKDLKLQ FDLLDSLPT 
LKIGRKSKT IMQNMAEIE KLEQNYRIN RDLLKYEKE 
ERLKVQEEM CPESTFLAL CCLHEKKGK KERQKSPLK 
PKDFIHRNI ELATAMAKL PLTDEEKHQ LDQLLANLD 
DCGDQSNGF QMCESDKQR LAEIDTELE DKYPESRHL 
RSPSKNALL DEALKEIDG KIATVRDE
      CDS sequence
      • >TC015958 RA
        atggatgca gtaccggaa atcaaaggc atttcatcc 
gagctgttg gaaaaactg gaaaaattg cagaaacaa 
gacttattt tcattttta ccagacgtg gaaacggac 
ctgaaacta ccaccgctg cctcaagat ttgaaacta 
caatttgac ttgcttgac tcccttccc acactaaaa 
atcggccgt aagagcaag accatcatg caaaacatg 
gccgaaatt gaaaagctc gagcaaaac taccgcata 
aacagagac ctgttaaag tacgaaaaa gaggagcgc 
ttgaaagtg caagaagaa atgtgcccc gagtccaca 
ttcctagct ttatgctgc ctccatgaa aagaaaggc 
aagaaagag cgccaaaaa tcgcccctc aaacccaag 
gacttcatc caccgaaac atcgagctg gctacagcc 
atggcgaaa ttgcctctc actgacgaa gagaagcac 
cagttggac cagctctta gcaaacctt gacgattgc 
ggcgatcaa tcgaacggc ttccaaatg tgcgaaagc 
gacaaacaa cgtttggcc gaaatcgat accgaactt 
gaagataaa taccccgag agtcgacac ttgcggtcg 
ccttcgaaa aatgcgctc ttggacgag gctttgaag 
gagatcgat ggcaaaata gccaccgtt cgggacgaa 
taa
      • >TC015958 RB
        atggatgca gtaccggaa atcaaaggc atttcatcc 
gagctgttg gaaaaactg gaaaaattg cagaaacaa 
gacttattt tcattttta ccagacgtg gaaacggac 
ctgaaacta ccaccgctg cctcaaggt ttgcccaac 
tttaattct gcttttttt aattattcc aaagatttg 
aaactacaa tttgacttg cttgactcc cttcccaca 
ctaaaaatc ggccgtaag agcaagacc atcatgcaa 
aacatggcc gaaattgaa aagctcgag caaaactac 
cgcataaac agagacctg ttaaagtac gaaaaagag 
gagcgcttg aaagtgcaa gaagaaatg tgccccgag 
tccacattc ctagcttta tgctgcctc catgaaaag 
aaaggcaag aaagagcgc caaaaatcg cccctcaaa 
cccaaggac ttcatccac cgaaacatc gagctggct 
acagccatg gcgaaattg cctctcact gacgaagag 
aagcaccag ttggaccag ctcttagca aaccttgac 
gattgcggc gatcaatcg aacggcttc caaatgtgc 
gaaagcgac aaacaacgt ttggccgaa atcgatacc 
gaacttgaa gataaatac cccgagagt cgacacttg 
cggtcgcct tcgaaaaat gcgctcttg gacgaggct 
ttgaaggag atcgatggc aaaatagcc accgttcgg 
gacgaataa
      mRNA sequence
      • >TC015958 RA
        tgaataatc ttgtcaaat tttgcgaaa atttactac 
acgtaataa gttattttt tgccaaaat ggatgcagt 
accggaaat caaaggcat ttcatccga gctgttgga 
aaaactgga aaaattgca gaaacaaga cttattttc 
atttttacc agacgtgga aacggacct gaaactacc 
accgctgcc tcaagattt gaaactaca atttgactt 
gcttgactc ccttcccac actaaaaat cggccgtaa 
gagcaagac catcatgca aaacatggc cgaaattga 
aaagctcga gcaaaacta ccgcataaa cagagacct 
gttaaagta cgaaaaaga ggagcgctt gaaagtgca 
agaagaaat gtgccccga gtccacatt cctagcttt 
atgctgcct ccatgaaaa gaaaggcaa gaaagagcg 
ccaaaaatc gcccctcaa acccaagga cttcatcca 
ccgaaacat cgagctggc tacagccat ggcgaaatt 
gcctctcac tgacgaaga gaagcacca gttggacca 
gctcttagc aaaccttga cgattgcgg cgatcaatc 
gaacggctt ccaaatgtg cgaaagcga caaacaacg 
tttggccga aatcgatac cgaacttga agataaata 
ccccgagag tcgacactt gcggtcgcc ttcgaaaaa 
tgcgctctt ggacgaggc tttgaagga gatcgatgg 
caaaatagc caccgttcg ggacgaata aatgaataa 
aaatcgact tgtcga
      • >TC015958 RB
        tgaataatc ttgtcaaat tttgcgaaa atttactac 
acgtaataa gttattttt tgccaaaat ggatgcagt 
accggaaat caaaggcat ttcatccga gctgttgga 
aaaactgga aaaattgca gaaacaaga cttattttc 
atttttacc agacgtgga aacggacct gaaactacc 
accgctgcc tcaaggttt gcccaactt taattctgc 
tttttttaa ttattccaa agatttgaa actacaatt 
tgacttgct tgactccct tcccacact aaaaatcgg 
ccgtaagag caagaccat catgcaaaa catggccga 
aattgaaaa gctcgagca aaactaccg cataaacag 
agacctgtt aaagtacga aaaagagga gcgcttgaa 
agtgcaaga agaaatgtg ccccgagtc cacattcct 
agctttatg ctgcctcca tgaaaagaa aggcaagaa 
agagcgcca aaaatcgcc cctcaaacc caaggactt 
catccaccg aaacatcga gctggctac agccatggc 
gaaattgcc tctcactga cgaagagaa gcaccagtt 
ggaccagct cttagcaaa ccttgacga ttgcggcga 
tcaatcgaa cggcttcca aatgtgcga aagcgacaa 
acaacgttt ggccgaaat cgataccga acttgaaga 
taaataccc cgagagtcg acacttgcg gtcgccttc 
gaaaaatgc gctcttgga cgaggcttt gaaggagat 
cgatggcaa aatagccac cgttcggga cgaataaat 
gaataaaaa tcgacttgt cga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY