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Gene information
General information
TC015958 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3939543..3940495
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015958
      iBeetle screen iB_05932

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015958 RA
        MDAVPEIKG ISSELLEKL EKLQKQDLF 
        SFLPDVETD LKLPPLPQD LKLQFDLLD 
        SLPTLKIGR KSKTIMQNM AEIEKLEQN 
        YRINRDLLK YEKEERLKV QEEMCPEST 
        FLALCCLHE KKGKKERQK SPLKPKDFI 
        HRNIELATA MAKLPLTDE EKHQLDQLL 
        ANLDDCGDQ SNGFQMCES DKQRLAEID 
        TELEDKYPE SRHLRSPSK NALLDEALK 
        EIDGKIATV RDE
      • >TC015958 RB
        MDAVPEIKG ISSELLEKL EKLQKQDLF SFLPDVETD 
        LKLPPLPQG LPNFNSAFF NYSKDLKLQ FDLLDSLPT 
        LKIGRKSKT IMQNMAEIE KLEQNYRIN RDLLKYEKE 
        ERLKVQEEM CPESTFLAL CCLHEKKGK KERQKSPLK 
        PKDFIHRNI ELATAMAKL PLTDEEKHQ LDQLLANLD 
        DCGDQSNGF QMCESDKQR LAEIDTELE DKYPESRHL 
        RSPSKNALL DEALKEIDG KIATVRDE
      CDS sequence
      • >TC015958 RA
        atggatgca gtaccggaa atcaaaggc atttcatcc 
        gagctgttg gaaaaactg gaaaaattg cagaaacaa 
        gacttattt tcattttta ccagacgtg gaaacggac 
        ctgaaacta ccaccgctg cctcaagat ttgaaacta 
        caatttgac ttgcttgac tcccttccc acactaaaa 
        atcggccgt aagagcaag accatcatg caaaacatg 
        gccgaaatt gaaaagctc gagcaaaac taccgcata 
        aacagagac ctgttaaag tacgaaaaa gaggagcgc 
        ttgaaagtg caagaagaa atgtgcccc gagtccaca 
        ttcctagct ttatgctgc ctccatgaa aagaaaggc 
        aagaaagag cgccaaaaa tcgcccctc aaacccaag 
        gacttcatc caccgaaac atcgagctg gctacagcc 
        atggcgaaa ttgcctctc actgacgaa gagaagcac 
        cagttggac cagctctta gcaaacctt gacgattgc 
        ggcgatcaa tcgaacggc ttccaaatg tgcgaaagc 
        gacaaacaa cgtttggcc gaaatcgat accgaactt 
        gaagataaa taccccgag agtcgacac ttgcggtcg 
        ccttcgaaa aatgcgctc ttggacgag gctttgaag 
        gagatcgat ggcaaaata gccaccgtt cgggacgaa 
        taa
      • >TC015958 RB
        atggatgca gtaccggaa atcaaaggc atttcatcc 
        gagctgttg gaaaaactg gaaaaattg cagaaacaa 
        gacttattt tcattttta ccagacgtg gaaacggac 
        ctgaaacta ccaccgctg cctcaaggt ttgcccaac 
        tttaattct gcttttttt aattattcc aaagatttg 
        aaactacaa tttgacttg cttgactcc cttcccaca 
        ctaaaaatc ggccgtaag agcaagacc atcatgcaa 
        aacatggcc gaaattgaa aagctcgag caaaactac 
        cgcataaac agagacctg ttaaagtac gaaaaagag 
        gagcgcttg aaagtgcaa gaagaaatg tgccccgag 
        tccacattc ctagcttta tgctgcctc catgaaaag 
        aaaggcaag aaagagcgc caaaaatcg cccctcaaa 
        cccaaggac ttcatccac cgaaacatc gagctggct 
        acagccatg gcgaaattg cctctcact gacgaagag 
        aagcaccag ttggaccag ctcttagca aaccttgac 
        gattgcggc gatcaatcg aacggcttc caaatgtgc 
        gaaagcgac aaacaacgt ttggccgaa atcgatacc 
        gaacttgaa gataaatac cccgagagt cgacacttg 
        cggtcgcct tcgaaaaat gcgctcttg gacgaggct 
        ttgaaggag atcgatggc aaaatagcc accgttcgg 
        gacgaataa
      mRNA sequence
      • >TC015958 RA
        tgaataatc ttgtcaaat tttgcgaaa atttactac 
        acgtaataa gttattttt tgccaaaat ggatgcagt 
        accggaaat caaaggcat ttcatccga gctgttgga 
        aaaactgga aaaattgca gaaacaaga cttattttc 
        atttttacc agacgtgga aacggacct gaaactacc 
        accgctgcc tcaagattt gaaactaca atttgactt 
        gcttgactc ccttcccac actaaaaat cggccgtaa 
        gagcaagac catcatgca aaacatggc cgaaattga 
        aaagctcga gcaaaacta ccgcataaa cagagacct 
        gttaaagta cgaaaaaga ggagcgctt gaaagtgca 
        agaagaaat gtgccccga gtccacatt cctagcttt 
        atgctgcct ccatgaaaa gaaaggcaa gaaagagcg 
        ccaaaaatc gcccctcaa acccaagga cttcatcca 
        ccgaaacat cgagctggc tacagccat ggcgaaatt 
        gcctctcac tgacgaaga gaagcacca gttggacca 
        gctcttagc aaaccttga cgattgcgg cgatcaatc 
        gaacggctt ccaaatgtg cgaaagcga caaacaacg 
        tttggccga aatcgatac cgaacttga agataaata 
        ccccgagag tcgacactt gcggtcgcc ttcgaaaaa 
        tgcgctctt ggacgaggc tttgaagga gatcgatgg 
        caaaatagc caccgttcg ggacgaata aatgaataa 
        aaatcgact tgtcga
      • >TC015958 RB
        tgaataatc ttgtcaaat tttgcgaaa atttactac 
        acgtaataa gttattttt tgccaaaat ggatgcagt 
        accggaaat caaaggcat ttcatccga gctgttgga 
        aaaactgga aaaattgca gaaacaaga cttattttc 
        atttttacc agacgtgga aacggacct gaaactacc 
        accgctgcc tcaaggttt gcccaactt taattctgc 
        tttttttaa ttattccaa agatttgaa actacaatt 
        tgacttgct tgactccct tcccacact aaaaatcgg 
        ccgtaagag caagaccat catgcaaaa catggccga 
        aattgaaaa gctcgagca aaactaccg cataaacag 
        agacctgtt aaagtacga aaaagagga gcgcttgaa 
        agtgcaaga agaaatgtg ccccgagtc cacattcct 
        agctttatg ctgcctcca tgaaaagaa aggcaagaa 
        agagcgcca aaaatcgcc cctcaaacc caaggactt 
        catccaccg aaacatcga gctggctac agccatggc 
        gaaattgcc tctcactga cgaagagaa gcaccagtt 
        ggaccagct cttagcaaa ccttgacga ttgcggcga 
        tcaatcgaa cggcttcca aatgtgcga aagcgacaa 
        acaacgttt ggccgaaat cgataccga acttgaaga 
        taaataccc cgagagtcg acacttgcg gtcgccttc 
        gaaaaatgc gctcttgga cgaggcttt gaaggagat 
        cgatggcaa aatagccac cgttcggga cgaataaat 
        gaataaaaa tcgacttgt cga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY