Status
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Gene information
General information
TC015946 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4070735..4075755
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015946
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05929

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    yolk late amount decreased - Number of animals showing the phenotype: 3

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015946 RA
      MDVHYSTDR LREVWQAVD QLHLSYLEM EESPEKLDH 
      RIRLEDCIR EYLCMALHS QKFVLRETG EVLYRSAST 
      KRDFSGYKA ATGWNAIQI YAGNLLSQP WRKEYRQIK 
      TYCGFYKHQ IEANLVGAE IMFEVMGYR NLSNGVMVL 
      EGPICPDTV TNVSKDCLI AYVECQILK HIWEEISPT 
      FKITWLEVL EFRKAHLCT PEHSIRALT YQFYEKQFQ 
      AQNSDYSRC HQTPVAPAV QYPPQITPH NHVPVTPMP 
      YVYAPTCCP NNYATYAHV PYSVPLQYP PHVVKPSFS 
      NGYYCANGY INQQPPSYE YGVPTGQLI ELDSHHNGG 
      YDAVDRPTN SLRVHRNSL SNHEKKVNE AKTVNSKEL 
      EKPESHFED WDYVYRNLE SQGYSKDLG ERGDILGQS 
      AEHKKSKCT NLDEAMHNL TITERPLKI SEALEKYDG 
      EPEPIRKNV RKHSQSSSY ENLSEIKKA NVAKPAKTK 
      TPTVRDKPV NNNNYEAKN TNCKWECKA CTYHNETSK 
      DICEMCSKS RSNVEQQME IGGPQCSKC TLVNLKEAK 
      VCQACGASL KDSPTYI
    CDS sequence
    • >TC015946 RA
      atggacgtg cattattcg actgacaga ctcagagaa 
      gtgtggcaa gcagttgac caacttcat ctgagttac 
      ttggaaatg gaggaaagt cccgagaaa ctcgaccac 
      aggatacgt ctggaagat tgtattcgt gaatatttg 
      tgtatggcc ttgcactca cagaaattt gttttgcga 
      gaaactggg gaggttttg tatcgctct gcaagcact 
      aaaagggat tttagcggc tataaggcg gccacaggc 
      tggaatgct attcaaata tatgccggg aatttgttg 
      tcacagccg tggcggaag gaatacaga caaataaaa 
      acatattgc ggcttttac aagcaccaa atcgaggcg 
      aatcttgtg ggggctgaa attatgttc gaagtgatg 
      gggtacagg aacctcagt aatggtgtt atggtcctg 
      gagggaccc atttgtccc gatacagtc accaatgtt 
      tctaaagat tgccttata gcttatgta gagtgccaa 
      attttgaaa cacatctgg gaggagatt tcgccaacc 
      ttcaaaata acttggttg gaggtttta gaattccgc 
      aaagcacat ctttgtacc ccagaacac tccattagg 
      gctctaact taccaattt tacgagaag caatttcaa 
      gcacaaaac tctgactac agcaggtgt caccaaaca 
      ccagtcgct cccgcagtt cagtatccc ccccaaata 
      acaccccat aatcatgtt cctgtcact ccaatgccc 
      tacgtttac gctccaact tgttgtccg aacaattac 
      gctacttat gctcatgta ccttattcc gtgcccttg 
      caatatcca ccacacgtt gtaaaaccc agcttttcc 
      aatgggtat tactgcgca aatggttac ataaatcaa 
      cagcccccg tcatatgag tatggggtt cccacagga 
      cagttaata gagctagat tcgcatcat aacgggggc 
      tatgacgct gtcgatcgc cccactaat agtttaaga 
      gtgcatagg aattcgttg agtaatcac gagaagaaa 
      gtcaatgag gcgaagact gtgaacagc aaagaactg 
      gaaaaaccg gagtcgcac ttcgaagac tgggactac 
      gtctaccgc aacctggaa agccaaggc tactcgaaa 
      gacttgggc gagcgcgga gacatccta ggccaatcg 
      gccgagcac aaaaagtca aaatgtaca aatctggac 
      gaagccatg cacaacctg acaataaca gagcggccg 
      cttaaaatc agcgaagct ttagaaaaa tacgacgga 
      gagccggag ccaattagg aaaaacgtc cggaagcac 
      tcgcaaagc agctcgtac gagaacttg tccgaaatt 
      aagaaagca aatgttgcg aaaccggca aagacgaaa 
      acgccgact gtccgggac aagccggtc aataacaac 
      aactacgag gcgaagaac actaattgc aagtgggag 
      tgcaaggcc tgcacgtat cacaacgag acgtccaag 
      gacatctgc gaaatgtgc agcaaaagt cggagcaat 
      gtggagcag cagatggag atcgggggg ccccagtgc 
      tcgaagtgt acgctggtt aatttgaag gaggcgaag 
      gtgtgccag gcctgcggg gcgagtttg aaggactca 
      ccgacttat atttag
    mRNA sequence
    • >TC015946 RA
      tccctgcta ggtttattt tggttgttt gtgaaatta 
      ggttagtta tctgtcaaa gtttattaa tcaattcta 
      ttacacaaa aagacgctt tgtggcaat tttctcaaa 
      ggtttttcc ctttaaatg ctcattaga attagtgca 
      aattaacta atatggtaa ttatggacg tgcattatt 
      cgactgaca gactcagag aagtgtggc aagcagttg 
      accaacttc atctgagtt acttggaaa tggaggaaa 
      gtcccgaga aactcgacc acaggatac gtctggaag 
      attgtattc gtgaatatt tgtgtatgg ccttgcact 
      cacagaaat ttgttttgc gagaaactg gggaggttt 
      tgtatcgct ctgcaagca ctaaaaggg attttagcg 
      gctataagg cggccacag gctggaatg ctattcaaa 
      tatatgccg ggaatttgt tgtcacagc cgtggcgga 
      aggaataca gacaaataa aaacatatt gcggctttt 
      acaagcacc aaatcgagg cgaatcttg tgggggctg 
      aaattatgt tcgaagtga tggggtaca ggaacctca 
      gtaatggtg ttatggtcc tggagggac ccatttgtc 
      ccgatacag tcaccaatg tttctaaag attgcctta 
      tagcttatg tagagtgcc aaattttga aacacatct 
      gggaggaga tttcgccaa ccttcaaaa taacttggt 
      tggaggttt tagaattcc gcaaagcac atctttgta 
      ccccagaac actccatta gggctctaa cttaccaat 
      tttacgaga agcaatttc aagcacaaa actctgact 
      acagcaggt gtcaccaaa caccagtcg ctcccgcag 
      ttcagtatc ccccccaaa taacacccc ataatcatg 
      ttcctgtca ctccaatgc cctacgttt acgctccaa 
      cttgttgtc cgaacaatt acgctactt atgctcatg 
      taccttatt ccgtgccct tgcaatatc caccacacg 
      ttgtaaaac ccagctttt ccaatgggt attactgcg 
      caaatggtt acataaatc aacagcccc cgtcatatg 
      agtatgggg ttcccacag gacagttaa tagagctag 
      attcgcatc ataacgggg gctatgacg ctgtcgatc 
      gccccacta atagtttaa gagtgcata ggaattcgt 
      tgagtaatc acgagaaga aagtcaatg aggcgaaga 
      ctgtgaaca gcaaagaac tggaaaaac cggagtcgc 
      acttcgaag actgggact acgtctacc gcaacctgg 
      aaagccaag gctactcga aagacttgg gcgagcgcg 
      gagacatcc taggccaat cggccgagc acaaaaagt 
      caaaatgta caaatctgg acgaagcca tgcacaacc 
      tgacaataa cagagcggc cgcttaaaa tcagcgaag 
      ctttagaaa aatacgacg gagagccgg agccaatta 
      ggaaaaacg tccggaagc actcgcaaa gcagctcgt 
      acgagaact tgtccgaaa ttaagaaag caaatgttg 
      cgaaaccgg caaagacga aaacgccga ctgtccggg 
      acaagccgg tcaataaca acaactacg aggcgaaga 
      acactaatt gcaagtggg agtgcaagg cctgcacgt 
      atcacaacg agacgtcca aggacatct gcgaaatgt 
      gcagcaaaa gtcggagca atgtggagc agcagatgg 
      agatcgggg ggccccagt gctcgaagt gtacgctgg 
      ttaatttga aggaggcga aggtgtgcc aggcctgcg 
      gggcgagtt tgaaggact caccgactt atatttagt 
      tgtgaaaac agggttgtg taattttgt acggttcag 
      ttgctttcg gaatgttta ggttttttt atgaaaact 
      attttttat aacgaggat tgcttacat tttattgta 
      ctgtaaaat atgctatat ttattgcgc ttgctcttg 
      ttattcatt cttgtttgt tgactcatc aaataaatt 
      tatttagtg taagtgtaa gataatttg tttcttaat 
      ctgatggca tgcattaac atgtgacta aagcagaaa 
      acgacgaat aaaaaacag atgaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY