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Gene information
General information
TC015943 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4093827..4097394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015943
    iBeetle screen iB_05927

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015943 RA
      MVKNKINWK PVPVEDPNL NSEGLVAFE ECTDYGLVN 
      LEKPRKINR SKISKKHKS PVVVEKPAK KKRKVSSVA 
      LTPSKQGSS VETRVEINE KIQKSDSYL VWSNFGLPD 
      SIIKALVLQ GFNEPTLIQ SLSLPAAVL GRRDIVGAA 
      ETGSGKTLA FGLPIVAGI LNEKSKVVG NSDKKLYAL 
      VLTPTRELA VQVRDHLKA IVKFTDINI AVVLGGMAA 
      VKQERILSK RPEIVVATP GRLWELIQQ GNEHLSQIN 
      DIRYLAIDE TDRMLEKGH FEELHNILE RLNLDKTRA 
      KQRQNFVFS ATLTLVHDL PKYRFNKSK VSKMTPQQK 
      LSRIMTDLG IKNPKIVDI SQGGNTPAT LTESRISCG 
      IEEKDYYVY YFLQKHPGR TLIFCNSIG CVRRLANLL 
      GILGCRPLP LHASMQQRQ RLKNLERFR DDEHGILVA 
      TDVAARGLD IPKIEHVLH YQTPRTSES YVHRSGRTA 
      RATQQGLTV VLMEPSEIQ NYIKICKTL NRSEDLPIF 
      PVQEEYLKA VKQRVNLAR ELDKLELQI RKGNSEEGW 
      LRKAAKEMD IIVDDFSKK FDSDEISSQ KKLADVKRK 
      QLTALLTKP IFPKGFSGK YPLISGEFS PNLETNESA 
      LQTVKNAVE KELKSKKRI PLYKPKKTA NNPK
    CDS sequence
    • >TC015943 RA
      atggttaaa aacaaaata aattggaaa ccagtccct 
      gtggaagac ccgaatctc aattccgag ggtttagtc 
      gcgttcgag gaatgtaca gattacggt ttggtaaat 
      ttggaaaaa ccaagaaag ataaaccgc tccaagatt 
      tccaagaag cataaaagc cctgtcgtg gttgaaaaa 
      ccggccaag aaaaaacgg aaagtgtct tccgtggct 
      ctaacgccc tcgaaacag ggcagtagt gttgagacg 
      cgcgttgaa attaacgaa aaaatccaa aaatcggac 
      agttatcta gtgtggagt aacttcggg ttgcctgac 
      tcaattatc aaggcgctc gttttgcaa ggttttaac 
      gaaccaacg ctgattcag tcgctttcg ttacctgcg 
      gccgttttg gggcgcagg gacattgtg ggggctgcg 
      gagacggga agtgggaaa acgcttgcg tttggtttg 
      ccaattgtg gccggaatt ttgaacgaa aaaagtaaa 
      gttgtggga aactcggat aaaaaattg tatgcactt 
      gtcttgaca ccgacaagg gaactggcg gtgcaagtg 
      cgagaccac ttaaaagcc attgtaaaa tttacagat 
      ataaatatt gcggtggtt cttgggggc atggctgcc 
      gtaaaacag gagcgaatt ttgagtaag agaccagaa 
      attgtggtc gcaactcca ggccgtttg tgggaatta 
      atccaacaa ggcaatgaa catttgtcg caaataaac 
      gatattaga tatttagca attgacgaa acggaccga 
      atgcttgaa aagggccac tttgaggag ctgcacaac 
      atattagaa cgtctcaac cttgataaa acaagagcg 
      aaacaaaga caaaatttc gttttttct gcaacgctc 
      actctagta cacgatttg ccgaaatac cgcttcaat 
      aaatcaaag gtttcgaaa atgacaccg caacaaaaa 
      cttagcagg attatgacc gatttgggc ataaaaaac 
      ccgaaaatt gtggacata agtcaaggt ggaaacaca 
      ccggcgact ttaaccgaa agccgaatc agttgcggg 
      attgaggag aaggactat tacgtgtat tatttcctg 
      cagaagcat ccaggcagg actttgatt ttttgtaat 
      tcgatcgga tgtgtgcga cgtttggcg aatttgttg 
      ggcattttg gggtgtcgg cctttgccc cttcacgcg 
      tccatgcag cagagacag cgtttgaaa aatttggaa 
      cgttttagg gacgatgaa cacggaatt ttggtggcc 
      actgatgtg gcagctaga ggcttggat attccgaaa 
      atcgagcat gttttgcat tatcagaca cccagaaca 
      agtgagagt tatgttcat aggtctggg cgcacagct 
      cgtgcgact cagcaaggt ctcactgta gttttaatg 
      gagccttcg gaaatacag aactacata aaaatctgc 
      aaaactttg aatagaagt gaagatttg ccgattttt 
      ccggtacag gaagagtat ttaaaagcc gtcaaacag 
      cgagtgaat ttagcacga gaactggac aaattagaa 
      ttacagata cggaaggga aattcggag gaagggtgg 
      ttgcgtaaa gcggctaaa gaaatggat attatcgtc 
      gatgatttt tcaaaaaaa ttcgattct gacgaaatt 
      tcgtcacag aaaaagctc gcagacgtt aagagaaaa 
      cagttaact gcccttctc accaaaccc attttcccg 
      aagggtttt agcggaaaa tatccgctt atttccggc 
      gaattttcc ccaaatttg gaaaccaac gaatcggct 
      ctacaaact gtgaaaaac gctgtagag aaagaattg 
      aagtcgaaa aagcggata ccactttac aaacctaaa 
      aaaactgcg aacaatcca aaataa
    mRNA sequence
    • >TC015943 RA
      ataaaagtg tggttagaa ttttttatg taaaatggt 
      taaaaacaa aataaattg gaaaccagt ccctgtgga 
      agacccgaa tctcaattc cgagggttt agtcgcgtt 
      cgaggaatg tacagatta cggtttggt aaatttgga 
      aaaaccaag aaagataaa ccgctccaa gatttccaa 
      gaagcataa aagccctgt cgtggttga aaaaccggc 
      caagaaaaa acggaaagt gtcttccgt ggctctaac 
      gccctcgaa acagggcag tagtgttga gacgcgcgt 
      tgaaattaa cgaaaaaat ccaaaaatc ggacagtta 
      tctagtgtg gagtaactt cgggttgcc tgactcaat 
      tatcaaggc gctcgtttt gcaaggttt taacgaacc 
      aacgctgat tcagtcgct ttcgttacc tgcggccgt 
      tttggggcg cagggacat tgtgggggc tgcggagac 
      gggaagtgg gaaaacgct tgcgtttgg tttgccaat 
      tgtggccgg aattttgaa cgaaaaaag taaagttgt 
      gggaaactc ggataaaaa attgtatgc acttgtctt 
      gacaccgac aagggaact ggcggtgca agtgcgaga 
      ccacttaaa agccattgt aaaatttac agatataaa 
      tattgcggt ggttcttgg gggcatggc tgccgtaaa 
      acaggagcg aattttgag taagagacc agaaattgt 
      ggtcgcaac tccaggccg tttgtggga attaatcca 
      acaaggcaa tgaacattt gtcgcaaat aaacgatat 
      tagatattt agcaattga cgaaacgga ccgaatgct 
      tgaaaaggg ccactttga ggagctgca caacatatt 
      agaacgtct caaccttga taaaacaag agcgaaaca 
      aagacaaaa tttcgtttt ttctgcaac gctcactct 
      agtacacga tttgccgaa ataccgctt caataaatc 
      aaaggtttc gaaaatgac accgcaaca aaaacttag 
      caggattat gaccgattt gggcataaa aaacccgaa 
      aattgtgga cataagtca aggtggaaa cacaccggc 
      gactttaac cgaaagccg aatcagttg cgggattga 
      ggagaagga ctattacgt gtattattt cctgcagaa 
      gcatccagg caggacttt gattttttg taattcgat 
      cggatgtgt gcgacgttt ggcgaattt gttgggcat 
      tttggggtg tcggccttt gccccttca cgcgtccat 
      gcagcagag acagcgttt gaaaaattt ggaacgttt 
      tagggacga tgaacacgg aattttggt ggccactga 
      tgtggcagc tagaggctt ggatattcc gaaaatcga 
      gcatgtttt gcattatca gacacccag aacaagtga 
      gagttatgt tcataggtc tgggcgcac agctcgtgc 
      gactcagca aggtctcac tgtagtttt aatggagcc 
      ttcggaaat acagaacta cataaaaat ctgcaaaac 
      tttgaatag aagtgaaga tttgccgat ttttccggt 
      acaggaaga gtatttaaa agccgtcaa acagcgagt 
      gaatttagc acgagaact ggacaaatt agaattaca 
      gatacggaa gggaaattc ggaggaagg gtggttgcg 
      taaagcggc taaagaaat ggatattat cgtcgatga 
      tttttcaaa aaaattcga ttctgacga aatttcgtc 
      acagaaaaa gctcgcaga cgttaagag aaaacagtt 
      aactgccct tctcaccaa acccatttt cccgaaggg 
      ttttagcgg aaaatatcc gcttatttc cggcgaatt 
      ttccccaaa tttggaaac caacgaatc ggctctaca 
      aactgtgaa aaacgctgt agagaaaga attgaagtc 
      gaaaaagcg gataccact ttacaaacc taaaaaaac 
      tgcgaacaa tccaaaata aaagttttt attacaata 
      aaatggatt aatcaatca tggtaacaa cgaccctgc 
      gccgtttct tcgcggaag acgtattgg gagttttct 
      gttcaccgt tatttgttt ccgacactt tatccccac 
      ttatcgaaa gatgcaagt cttccaaaa attgcattt 
      ctagaggcg ttggcctga caggcgttg aagtacatt 
      gttgctcgt tttgggggt tttccgggt ccgatatgt 
      gtaaaatgg gtatttccc cttcgtccg ttaggttaa 
      cacaaccat cactgttga ataaatcgg aagggcaca 
      aagttttct cttttgcat gattttccc caggcactt 
      ggatgtttt cattggatt tcttcttcg tggcgcttc 
      gtaaaatac gacttgatt ccggttttt cctttccac 
      ctgaaagcc ttttctcct ttatctctt ttttatgga 
      ctgtgtctt cctccgttt tctctttct tgccagtgg 
      aatgccttt ttctcggag cactgtctt tgattttct 
      tcgatcctg tcacaactt tggcctttt ctgtgcttt 
      tcttacact ccttagctg gtatttctt cctcagtgt 
      gatccaaag ttaggtcca caattattg tttctttct 
      tgtcggttt gagtattat ccaattctt tcgcaatca 
      cggtgttat ccgtaaatt ctggcgttg cgaagcaat 
      tcatttttt catcaacag attcgctac taatgttta 
      aatcattca aaacgcaaa aaaccgcct cattttgtg 
      ctccgtcgc ccaattttt gtttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY