Status
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Gene information
General information
TC033615
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015936 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4145305..4148978
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033615
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033615 RA
      MDFQIPFVF VSLFSLASV LLIFVYKFG IKEKSYEEA 
LAEQRQQTQ ILLGVKPKL KEKKNKKTA KKVKEKLSL 
GENHETVEE PESEANQKS VESSKKLHV EFKEVPEEV 
SVKPPTPPA KKQSRKEKV RPILVKKDK LENGVEAVA 
DETPQTNHF EELHPKDDF ELKFRSVSV EETPNQEKN 
VAKVETDLH KKQPETETV PLSKENHQA VTSTINGFI 
SNTGKEKKK KKSEFNTLQ QLGDSNGPN LSLLLNLVR 
KAELSRSEV QILIDLLLN KQHEAPAVL DEWTEGKTD 
PVQKLKKQL AEKEKLLQE EQQIATSAQ NKLREIRAE 
NQAEKSRLQ QKIRNLEEL DQKKQLEAG RLHQQVQQL 
QAQLKDEVL KSHKLREEH AAIQMQRQQ IEIRLSQAQ 
ESDVIINRL QTEVQELGA LNNQLRMEL ARFTEENLA 
EKERSQSII LQLSNFQKE LEQKTDRNR QLEETRLKL 
ERDLDAILK QDNEYKAEI SRLTSVLQQ QNEEIRRME 
HGKKQHLDE LQKQKDEAN KMRNELRKA QEERMNGSL 
QENKQHEVE ILNLHNELS SVKTKLHQS ENDLKLAND 
TVETLQRKV DELQVRYQE EKTKVEEQK VKNNELRTK 
NWKVMEALN AAESRTKSH KTNIQSERE FIQRLFPEI 
KIEEISDGE NFVRNYIND LKKQNSKEG DSQLQLQLK 
HYKDVIDNT EKMLNKLQQ HIEQEEINW RMQLAAKTA 
ELEQLKENC GSELQEKLT SLELELKKE REDKKRVIA 
QLNDKTDLR ENSSNGLHL R
    CDS sequence
    • >TC033615 RA
      atggatttt caaataccg ttcgttttt gtgtcactc 
ttttccctc gccagcgtt ctgctgata tttgtgtac 
aaatttgga atcaaagaa aaaagttac gaagaggca 
ttggctgag caacgccaa caaactcaa attctgcta 
ggagtcaaa cccaaactc aaggagaaa aagaacaaa 
aagaccgcg aagaaggtc aaagaaaaa cttagtctt 
ggcgagaat cacgaaaca gtggaagag ccggaatcg 
gaagccaac cagaaaagt gtggaaagt agcaagaaa 
ctccacgtg gaattcaag gaggttcca gaagaagtc 
tcggtcaaa ccaccaact ccaccggca aagaaacaa 
tcaaggaag gaaaaagtt cgtcctatt ttggtcaaa 
aaagacaag cttgaaaac ggtgttgag gccgttgct 
gacgaaacg ccccaaacc aatcatttt gaagagctg 
caccctaag gacgatttt gagctaaaa ttccgttcc 
gtgagcgtt gaagagact ccaaatcag gagaaaaat 
gtcgctaaa gttgaaact gacctgcac aaaaaacaa 
cccgagacg gaaaccgtt ccgttgtct aaagaaaac 
caccaagcc gtcacttca accattaat ggttttatc 
agtaacacc ggaaaagaa aagaagaag aaaaaaagc 
gagttcaac actttgcaa caacttggt gatagtaac 
ggccccaac ttgtcgctt ctcctcaac ttggtgcgt 
aaggctgaa ttgtctcgt tctgaagtt cagatactt 
attgatctc ttactgaac aagcagcat gaggctcct 
gccgttttg gacgaatgg actgagggc aaaaccgat 
ccggtccaa aaactcaag aaacaacta gcagaaaaa 
gaaaaacta ctacaagaa gaacaacaa atcgccaca 
agtgcgcaa aacaaactt cgcgaaata cgcgccgaa 
aatcaggcc gaaaaatca cgcttgcag caaaaaatc 
cgaaacttg gaagaactt gaccagaaa aaacaactc 
gaagcgggg agacttcac caacaagtc cagcaattg 
caagcacag cttaaggac gaagtcctc aaaagccac 
aagttgagg gaggaacac gcagccatc cagatgcaa 
aggcaacag attgaaatc agactgagt caagcacag 
gagtcggac gttataata aatcggtta caaaccgaa 
gtacaagaa ttgggggct ttgaacaac caactacga 
atggaattg gctcgtttc actgaggag aatttggcg 
gagaaagaa cgcagccaa tcgattatt ttgcaactg 
agcaatttc caaaaagaa ctagagcaa aaaactgac 
cggaatcgc caattggag gagacacgt ttgaagttg 
gagcgcgac ttggacgca attctgaag caagacaac 
gaatacaaa gccgaaatt tcgcgtctg actagcgtc 
ctgcagcag caaaacgaa gaaatccga agaatggaa 
cacgggaag aagcaacac cttgacgag ttgcagaag 
caaaaggac gaagcaaat aaaatgagg aacgagttg 
agaaaagca caggaagag cgcatgaac ggatcgctg 
caagaaaac aagcaacac gaagtggaa attttgaat 
cttcacaat gagttatct tcggtgaaa acaaagtta 
caccagagt gaaaatgac ttgaaacta gccaatgac 
actgttgag acgttgcaa aggaaagtg gacgagttg 
caagtgagg tatcaagag gaaaagact aaagtagag 
gagcaaaaa gttaaaaat aatgagttg agaactaaa 
aattggaaa gttatggag gcgttaaat gctgctgaa 
agcaggacc aaaagtcac aaaaccaat attcaaagt 
gagagggaa tttattcag aggttgttc cccgaaatc 
aaaattgag gaaataagt gatggggaa aacttcgtc 
aggaattac attaatgac ttgaaaaaa caaaatagt 
aaagaaggt gatagtcag cttcagtta caactgaaa 
cattacaaa gatgttata gataatacg gaaaaaatg 
ttaaataag ttacaacaa cacattgaa caggaagaa 
attaactgg aggatgcag ttggcggcg aaaactgcc 
gagttagag caattgaaa gaaaattgc ggtagtgag 
ttacaagaa aaattgact tcgttggaa ttagaactg 
aagaaagaa cgtgaagat aaaaagaga gttattgca 
caacttaac gataaaacc gacttgaga gaaaattca 
tccaatgga ttgcacttg agatag
    mRNA sequence
    • >TC033615 RA
      ggtgttaaa aatcgccgt gtaaattac agttacgct 
gataatatt tatttgtgg tttgtactc gttttgtgt 
aaagtgtct tattttccc tgctattgc ggtagttaa 
ttaaatatt caatatgga ttttcaaat accgttcgt 
ttttgtgtc actcttttc cctcgccag cgttctgct 
gatatttgt gtacaaatt tggaatcaa agaaaaaag 
ttacgaaga ggcattggc tgagcaacg ccaacaaac 
tcaaattct gctaggagt caaacccaa actcaagga 
gaaaaagaa caaaaagac cgcgaagaa ggtcaaaga 
aaaacttag tcttggcga gaatcacga aacagtgga 
agagccgga atcggaagc caaccagaa aagtgtgga 
aagtagcaa gaaactcca cgtggaatt caaggaggt 
tccagaaga agtctcggt caaaccacc aactccacc 
ggcaaagaa acaatcaag gaaggaaaa agttcgtcc 
tattttggt caaaaaaga caagcttga aaacggtgt 
tgaggccgt tgctgacga aacgcccca aaccaatca 
ttttgaaga gctgcaccc taaggacga ttttgagct 
aaaattccg ttccgtgag cgttgaaga gactccaaa 
tcaggagaa aaatgtcgc taaagttga aactgacct 
gcacaaaaa acaacccga gacggaaac cgttccgtt 
gtctaaaga aaaccacca agccgtcac ttcaaccat 
taatggttt tatcagtaa caccggaaa agaaaagaa 
gaagaaaaa aagcgagtt caacacttt gcaacaact 
tggtgatag taacggccc caacttgtc gcttctcct 
caacttggt gcgtaaggc tgaattgtc tcgttctga 
agttcagat acttattga tctcttact gaacaagca 
gcatgaggc tcctgccgt tttggacga atggactga 
gggcaaaac cgatccggt ccaaaaact caagaaaca 
actagcaga aaaagaaaa actactaca agaagaaca 
acaaatcgc cacaagtgc gcaaaacaa acttcgcga 
aatacgcgc cgaaaatca ggccgaaaa atcacgctt 
gcagcaaaa aatccgaaa cttggaaga acttgacca 
gaaaaaaca actcgaagc ggggagact tcaccaaca 
agtccagca attgcaagc acagcttaa ggacgaagt 
cctcaaaag ccacaagtt gagggagga acacgcagc 
catccagat gcaaaggca acagattga aatcagact 
gagtcaagc acaggagtc ggacgttat aataaatcg 
gttacaaac cgaagtaca agaattggg ggctttgaa 
caaccaact acgaatgga attggctcg tttcactga 
ggagaattt ggcggagaa agaacgcag ccaatcgat 
tattttgca actgagcaa tttccaaaa agaactaga 
gcaaaaaac tgaccggaa tcgccaatt ggaggagac 
acgtttgaa gttggagcg cgacttgga cgcaattct 
gaagcaaga caacgaata caaagccga aatttcgcg 
tctgactag cgtcctgca gcagcaaaa cgaagaaat 
ccgaagaat ggaacacgg gaagaagca acaccttga 
cgagttgca gaagcaaaa ggacgaagc aaataaaat 
gaggaacga gttgagaaa agcacagga agagcgcat 
gaacggatc gctgcaaga aaacaagca acacgaagt 
ggaaatttt gaatcttca caatgagtt atcttcggt 
gaaaacaaa gttacacca gagtgaaaa tgacttgaa 
actagccaa tgacactgt tgagacgtt gcaaaggaa 
agtggacga gttgcaagt gaggtatca agaggaaaa 
gactaaagt agaggagca aaaagttaa aaataatga 
gttgagaac taaaaattg gaaagttat ggaggcgtt 
aaatgctgc tgaaagcag gaccaaaag tcacaaaac 
caatattca aagtgagag ggaatttat tcagaggtt 
gttccccga aatcaaaat tgaggaaat aagtgatgg 
ggaaaactt cgtcaggaa ttacattaa tgacttgaa 
aaaacaaaa tagtaaaga aggtgatag tcagcttca 
gttacaact gaaacatta caaagatgt tatagataa 
tacggaaaa aatgttaaa taagttaca acaacacat 
tgaacagga agaaattaa ctggaggat gcagttggc 
ggcgaaaac tgccgagtt agagcaatt gaaagaaaa 
ttgcggtag tgagttaca agaaaaatt gacttcgtt 
ggaattaga actgaagaa agaacgtga agataaaaa 
gagagttat tgcacaact taacgataa aaccgactt 
gagagaaaa ttcatccaa tggattgca cttgagata 
gattggaat ttttcgctt ccaccagtc ttaatgata 
ggatttagt aaagataaa taatattgc ataactaat 
tacatttct ttaacttat tcatcattt gtagtaggt 
aatcgtttc atgtattta ctcagtcat tcctcgcat 
tgtgtgtgc aataagttt ataaatttc atactttat 
gataatagg tcacgatat ttttaataa aagcaattt 
tcaatt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY