General information | |||
TC033615
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 4145305..4148978 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC033615 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05926 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033615 RA
MDFQIPFVF VSLFSLASV LLIFVYKFG IKEKSYEEA LAEQRQQTQ ILLGVKPKL KEKKNKKTA KKVKEKLSL GENHETVEE PESEANQKS VESSKKLHV EFKEVPEEV SVKPPTPPA KKQSRKEKV RPILVKKDK LENGVEAVA DETPQTNHF EELHPKDDF ELKFRSVSV EETPNQEKN VAKVETDLH KKQPETETV PLSKENHQA VTSTINGFI SNTGKEKKK KKSEFNTLQ QLGDSNGPN LSLLLNLVR KAELSRSEV QILIDLLLN KQHEAPAVL DEWTEGKTD PVQKLKKQL AEKEKLLQE EQQIATSAQ NKLREIRAE NQAEKSRLQ QKIRNLEEL DQKKQLEAG RLHQQVQQL QAQLKDEVL KSHKLREEH AAIQMQRQQ IEIRLSQAQ ESDVIINRL QTEVQELGA LNNQLRMEL ARFTEENLA EKERSQSII LQLSNFQKE LEQKTDRNR QLEETRLKL ERDLDAILK QDNEYKAEI SRLTSVLQQ QNEEIRRME HGKKQHLDE LQKQKDEAN KMRNELRKA QEERMNGSL QENKQHEVE ILNLHNELS SVKTKLHQS ENDLKLAND TVETLQRKV DELQVRYQE EKTKVEEQK VKNNELRTK NWKVMEALN AAESRTKSH KTNIQSERE FIQRLFPEI KIEEISDGE NFVRNYIND LKKQNSKEG DSQLQLQLK HYKDVIDNT EKMLNKLQQ HIEQEEINW RMQLAAKTA ELEQLKENC GSELQEKLT SLELELKKE REDKKRVIA QLNDKTDLR ENSSNGLHL R
- >TC033615 RA
atggatttt caaataccg ttcgttttt gtgtcactc ttttccctc gccagcgtt ctgctgata tttgtgtac aaatttgga atcaaagaa aaaagttac gaagaggca ttggctgag caacgccaa caaactcaa attctgcta ggagtcaaa cccaaactc aaggagaaa aagaacaaa aagaccgcg aagaaggtc aaagaaaaa cttagtctt ggcgagaat cacgaaaca gtggaagag ccggaatcg gaagccaac cagaaaagt gtggaaagt agcaagaaa ctccacgtg gaattcaag gaggttcca gaagaagtc tcggtcaaa ccaccaact ccaccggca aagaaacaa tcaaggaag gaaaaagtt cgtcctatt ttggtcaaa aaagacaag cttgaaaac ggtgttgag gccgttgct gacgaaacg ccccaaacc aatcatttt gaagagctg caccctaag gacgatttt gagctaaaa ttccgttcc gtgagcgtt gaagagact ccaaatcag gagaaaaat gtcgctaaa gttgaaact gacctgcac aaaaaacaa cccgagacg gaaaccgtt ccgttgtct aaagaaaac caccaagcc gtcacttca accattaat ggttttatc agtaacacc ggaaaagaa aagaagaag aaaaaaagc gagttcaac actttgcaa caacttggt gatagtaac ggccccaac ttgtcgctt ctcctcaac ttggtgcgt aaggctgaa ttgtctcgt tctgaagtt cagatactt attgatctc ttactgaac aagcagcat gaggctcct gccgttttg gacgaatgg actgagggc aaaaccgat ccggtccaa aaactcaag aaacaacta gcagaaaaa gaaaaacta ctacaagaa gaacaacaa atcgccaca agtgcgcaa aacaaactt cgcgaaata cgcgccgaa aatcaggcc gaaaaatca cgcttgcag caaaaaatc cgaaacttg gaagaactt gaccagaaa aaacaactc gaagcgggg agacttcac caacaagtc cagcaattg caagcacag cttaaggac gaagtcctc aaaagccac aagttgagg gaggaacac gcagccatc cagatgcaa aggcaacag attgaaatc agactgagt caagcacag gagtcggac gttataata aatcggtta caaaccgaa gtacaagaa ttgggggct ttgaacaac caactacga atggaattg gctcgtttc actgaggag aatttggcg gagaaagaa cgcagccaa tcgattatt ttgcaactg agcaatttc caaaaagaa ctagagcaa aaaactgac cggaatcgc caattggag gagacacgt ttgaagttg gagcgcgac ttggacgca attctgaag caagacaac gaatacaaa gccgaaatt tcgcgtctg actagcgtc ctgcagcag caaaacgaa gaaatccga agaatggaa cacgggaag aagcaacac cttgacgag ttgcagaag caaaaggac gaagcaaat aaaatgagg aacgagttg agaaaagca caggaagag cgcatgaac ggatcgctg caagaaaac aagcaacac gaagtggaa attttgaat cttcacaat gagttatct tcggtgaaa acaaagtta caccagagt gaaaatgac ttgaaacta gccaatgac actgttgag acgttgcaa aggaaagtg gacgagttg caagtgagg tatcaagag gaaaagact aaagtagag gagcaaaaa gttaaaaat aatgagttg agaactaaa aattggaaa gttatggag gcgttaaat gctgctgaa agcaggacc aaaagtcac aaaaccaat attcaaagt gagagggaa tttattcag aggttgttc cccgaaatc aaaattgag gaaataagt gatggggaa aacttcgtc aggaattac attaatgac ttgaaaaaa caaaatagt aaagaaggt gatagtcag cttcagtta caactgaaa cattacaaa gatgttata gataatacg gaaaaaatg ttaaataag ttacaacaa cacattgaa caggaagaa attaactgg aggatgcag ttggcggcg aaaactgcc gagttagag caattgaaa gaaaattgc ggtagtgag ttacaagaa aaattgact tcgttggaa ttagaactg aagaaagaa cgtgaagat aaaaagaga gttattgca caacttaac gataaaacc gacttgaga gaaaattca tccaatgga ttgcacttg agatag
- >TC033615 RA
ggtgttaaa aatcgccgt gtaaattac agttacgct gataatatt tatttgtgg tttgtactc gttttgtgt aaagtgtct tattttccc tgctattgc ggtagttaa ttaaatatt caatatgga ttttcaaat accgttcgt ttttgtgtc actcttttc cctcgccag cgttctgct gatatttgt gtacaaatt tggaatcaa agaaaaaag ttacgaaga ggcattggc tgagcaacg ccaacaaac tcaaattct gctaggagt caaacccaa actcaagga gaaaaagaa caaaaagac cgcgaagaa ggtcaaaga aaaacttag tcttggcga gaatcacga aacagtgga agagccgga atcggaagc caaccagaa aagtgtgga aagtagcaa gaaactcca cgtggaatt caaggaggt tccagaaga agtctcggt caaaccacc aactccacc ggcaaagaa acaatcaag gaaggaaaa agttcgtcc tattttggt caaaaaaga caagcttga aaacggtgt tgaggccgt tgctgacga aacgcccca aaccaatca ttttgaaga gctgcaccc taaggacga ttttgagct aaaattccg ttccgtgag cgttgaaga gactccaaa tcaggagaa aaatgtcgc taaagttga aactgacct gcacaaaaa acaacccga gacggaaac cgttccgtt gtctaaaga aaaccacca agccgtcac ttcaaccat taatggttt tatcagtaa caccggaaa agaaaagaa gaagaaaaa aagcgagtt caacacttt gcaacaact tggtgatag taacggccc caacttgtc gcttctcct caacttggt gcgtaaggc tgaattgtc tcgttctga agttcagat acttattga tctcttact gaacaagca gcatgaggc tcctgccgt tttggacga atggactga gggcaaaac cgatccggt ccaaaaact caagaaaca actagcaga aaaagaaaa actactaca agaagaaca acaaatcgc cacaagtgc gcaaaacaa acttcgcga aatacgcgc cgaaaatca ggccgaaaa atcacgctt gcagcaaaa aatccgaaa cttggaaga acttgacca gaaaaaaca actcgaagc ggggagact tcaccaaca agtccagca attgcaagc acagcttaa ggacgaagt cctcaaaag ccacaagtt gagggagga acacgcagc catccagat gcaaaggca acagattga aatcagact gagtcaagc acaggagtc ggacgttat aataaatcg gttacaaac cgaagtaca agaattggg ggctttgaa caaccaact acgaatgga attggctcg tttcactga ggagaattt ggcggagaa agaacgcag ccaatcgat tattttgca actgagcaa tttccaaaa agaactaga gcaaaaaac tgaccggaa tcgccaatt ggaggagac acgtttgaa gttggagcg cgacttgga cgcaattct gaagcaaga caacgaata caaagccga aatttcgcg tctgactag cgtcctgca gcagcaaaa cgaagaaat ccgaagaat ggaacacgg gaagaagca acaccttga cgagttgca gaagcaaaa ggacgaagc aaataaaat gaggaacga gttgagaaa agcacagga agagcgcat gaacggatc gctgcaaga aaacaagca acacgaagt ggaaatttt gaatcttca caatgagtt atcttcggt gaaaacaaa gttacacca gagtgaaaa tgacttgaa actagccaa tgacactgt tgagacgtt gcaaaggaa agtggacga gttgcaagt gaggtatca agaggaaaa gactaaagt agaggagca aaaagttaa aaataatga gttgagaac taaaaattg gaaagttat ggaggcgtt aaatgctgc tgaaagcag gaccaaaag tcacaaaac caatattca aagtgagag ggaatttat tcagaggtt gttccccga aatcaaaat tgaggaaat aagtgatgg ggaaaactt cgtcaggaa ttacattaa tgacttgaa aaaacaaaa tagtaaaga aggtgatag tcagcttca gttacaact gaaacatta caaagatgt tatagataa tacggaaaa aatgttaaa taagttaca acaacacat tgaacagga agaaattaa ctggaggat gcagttggc ggcgaaaac tgccgagtt agagcaatt gaaagaaaa ttgcggtag tgagttaca agaaaaatt gacttcgtt ggaattaga actgaagaa agaacgtga agataaaaa gagagttat tgcacaact taacgataa aaccgactt gagagaaaa ttcatccaa tggattgca cttgagata gattggaat ttttcgctt ccaccagtc ttaatgata ggatttagt aaagataaa taatattgc ataactaat tacatttct ttaacttat tcatcattt gtagtaggt aatcgtttc atgtattta ctcagtcat tcctcgcat tgtgtgtgc aataagttt ataaatttc atactttat gataatagg tcacgatat ttttaataa aagcaattt tcaatt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
