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Gene information
General information
TC031190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4063167..4067764
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031190
    iBeetle screen iB_05922

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031190 RA
      MIVTFFMAE KRLTPRIKF IHTLHNQQN SRYPQYSST 
      LCSDSRCTV AITLSYIIP VVLCIPNFI TFTITGEEI 
      TENNEQVIL YHTDLTDTF KNDRALLRL QFWTYAVFM 
      KLLPCCILT VISLWLIYT LFKAKKRKQ VLHGYDCVP 
      LRAPKKKAS KAERRADRT TKMLVAVLT LFLITEFPQ 
      GIFALLIAI KGKDLFVRC YLLYGEIMD ILALINGAI 
      NFILYCCMN RMFRATFGQ LFKHKILAQ WAPSSEMHT 
      TIFVTFQDV GKWNACD
    • >TC031190 RB
      MIVTFFMAE KRLTPRIKF IHTLHNQQN SRYPQYSST 
      LCSDSRCTV AITLSYIIP VVLCIPNFI TFTITGEEI 
      TENNEQVIL YHTDLTDTF KNDRALLRL QFWTYAVFM 
      KLLPCCILT VISLWLIYT LFKAKKRKQ VLHGYDCVP 
      LRAPKKKAS KAERRADRT TKMLVAVLT LFLITEFPQ 
      GIFALLIAI KGKDLFVRC YLLYGEIMD ILALINGAI 
      NFILYCCMN RMFRATFGQ LFKHKILAQ WAPSSEMHT 
      TTSANGTHA TEL
    CDS sequence
    • >TC031190 RA
      atgattgtg acatttttt atggctgaa aaacgattg 
      acgcccaga attaaattt atccacact ctgcataat 
      cagcaaaat tccaggtat ccccagtac agcagcacc 
      ttgtgctcg gactcccgc tgcaccgtc gccatcacc 
      ctcagctac atcatcccc gtcgtgctc tgcatcccc 
      aacttcatc acgttcacc atcacaggc gaggaaatc 
      accgaaaac aacgagcaa gttatcctc taccacacc 
      gacctcacg gacactttc aagaacgat cgagccctt 
      ctgcgtctg cagttttgg acgtatgcc gtgttcatg 
      aaactcctc ccctgctgc atcctcacg gtgatcagt 
      ctctggctc atctacacc ctcttcaag gccaagaag 
      cgcaaacag gtgctgcac ggctacgac tgcgtgccg 
      ctccgcgcc cctaagaag aaggcgtcc aaggccgaa 
      cgtcgcgca gaccgcacc acgaaaatg ctggtggcg 
      gtgctgacc ctcttcctc atcaccgag ttcccccag 
      ggcatcttc gcgctgttg atcgccatc aaaggcaaa 
      gacttgttc gtccgatgc tacttgctg tacggcgaa 
      ataatggat attctagcg ctgatcaac ggcgccatc 
      aacttcatc ctctactgc tgcatgaac cgcatgttc 
      agggccacg tttggccaa ctctttaag cataaaatt 
      ctggcacag tgggccccc tcgtccgaa atgcacacg 
      acgattttt gttacgttt caggacgtc ggcaaatgg 
      aacgcatgc gactga
    • >TC031190 RB
      atgattgtg acatttttt atggctgaa aaacgattg 
      acgcccaga attaaattt atccacact ctgcataat 
      cagcaaaat tccaggtat ccccagtac agcagcacc 
      ttgtgctcg gactcccgc tgcaccgtc gccatcacc 
      ctcagctac atcatcccc gtcgtgctc tgcatcccc 
      aacttcatc acgttcacc atcacaggc gaggaaatc 
      accgaaaac aacgagcaa gttatcctc taccacacc 
      gacctcacg gacactttc aagaacgat cgagccctt 
      ctgcgtctg cagttttgg acgtatgcc gtgttcatg 
      aaactcctc ccctgctgc atcctcacg gtgatcagt 
      ctctggctc atctacacc ctcttcaag gccaagaag 
      cgcaaacag gtgctgcac ggctacgac tgcgtgccg 
      ctccgcgcc cctaagaag aaggcgtcc aaggccgaa 
      cgtcgcgca gaccgcacc acgaaaatg ctggtggcg 
      gtgctgacc ctcttcctc atcaccgag ttcccccag 
      ggcatcttc gcgctgttg atcgccatc aaaggcaaa 
      gacttgttc gtccgatgc tacttgctg tacggcgaa 
      ataatggat attctagcg ctgatcaac ggcgccatc 
      aacttcatc ctctactgc tgcatgaac cgcatgttc 
      agggccacg tttggccaa ctctttaag cataaaatt 
      ctggcacag tgggccccc tcgtccgaa atgcacacg 
      acgacgtcg gcaaatgga acgcatgcg actgaactg 
      tga
    mRNA sequence
    • >TC031190 RA
      actgatttt ggtaagatt tttgcggca actcacttt 
      ttcactgaa aactattta aaaaggtga ttatgattg 
      tgacatttt ttatggctg aaaaacgat tgacgccca 
      gaattaaat ttatccaca ctctgcata atcagcaaa 
      attccaggt atccccagt acagcagca ccttgtgct 
      cggactccc gctgcaccg tcgccatca ccctcagct 
      acatcatcc ccgtcgtgc tctgcatcc ccaacttca 
      tcacgttca ccatcacag gcgaggaaa tcaccgaaa 
      acaacgagc aagttatcc tctaccaca ccgacctca 
      cggacactt tcaagaacg atcgagccc ttctgcgtc 
      tgcagtttt ggacgtatg ccgtgttca tgaaactcc 
      tcccctgct gcatcctca cggtgatca gtctctggc 
      tcatctaca ccctcttca aggccaaga agcgcaaac 
      aggtgctgc acggctacg actgcgtgc cgctccgcg 
      cccctaaga agaaggcgt ccaaggccg aacgtcgcg 
      cagaccgca ccacgaaaa tgctggtgg cggtgctga 
      ccctcttcc tcatcaccg agttccccc agggcatct 
      tcgcgctgt tgatcgcca tcaaaggca aagacttgt 
      tcgtccgat gctacttgc tgtacggcg aaataatgg 
      atattctag cgctgatca acggcgcca tcaacttca 
      tcctctact gctgcatga accgcatgt tcagggcca 
      cgtttggcc aactcttta agcataaaa ttctggcac 
      agtgggccc cctcgtccg aaatgcaca cgacgattt 
      ttgttacgt ttcaggacg tcggcaaat ggaacgcat 
      gcgactgaa ctgtgaacg gttgttgtc tctagatat 
      tatcaatgt gaaaaatac aagtttata cttgag
    • >TC031190 RB
      actgatttt ggtaagatt tttgcggca actcacttt 
      ttcactgaa aactattta aaaaggtga ttatgattg 
      tgacatttt ttatggctg aaaaacgat tgacgccca 
      gaattaaat ttatccaca ctctgcata atcagcaaa 
      attccaggt atccccagt acagcagca ccttgtgct 
      cggactccc gctgcaccg tcgccatca ccctcagct 
      acatcatcc ccgtcgtgc tctgcatcc ccaacttca 
      tcacgttca ccatcacag gcgaggaaa tcaccgaaa 
      acaacgagc aagttatcc tctaccaca ccgacctca 
      cggacactt tcaagaacg atcgagccc ttctgcgtc 
      tgcagtttt ggacgtatg ccgtgttca tgaaactcc 
      tcccctgct gcatcctca cggtgatca gtctctggc 
      tcatctaca ccctcttca aggccaaga agcgcaaac 
      aggtgctgc acggctacg actgcgtgc cgctccgcg 
      cccctaaga agaaggcgt ccaaggccg aacgtcgcg 
      cagaccgca ccacgaaaa tgctggtgg cggtgctga 
      ccctcttcc tcatcaccg agttccccc agggcatct 
      tcgcgctgt tgatcgcca tcaaaggca aagacttgt 
      tcgtccgat gctacttgc tgtacggcg aaataatgg 
      atattctag cgctgatca acggcgcca tcaacttca 
      tcctctact gctgcatga accgcatgt tcagggcca 
      cgtttggcc aactcttta agcataaaa ttctggcac 
      agtgggccc cctcgtccg aaatgcaca cgacgacgt 
      cggcaaatg gaacgcatg cgactgaac tgtgaacgg 
      ttgttgtct ctagatatt atcaatgtg aaaaataca 
      agtttatac ttgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY