Status
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Gene information
General information
TC015882 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 395458..413165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015882
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015882 RA
      MSNEWGSQV EAMPSEKDL FIKITSKAL ENNMQERKL 
      VTYKQAQDN EGDRGGWGN KLDFLFSCI SLSVGLGNV 
      WRFPYLCYK NGGGAFIVT YIIAMIVCG IPMFFQEVA 
      IGQYLGSGG MTFVGQLCP ILKGVGYAA MTIVFLLDV 
      YYCIIISWT IFYLMSMFS YLPVLPWAG CDNWWNTAD 
      CFSSVNNST SNFNSSKFS TPVEEYFER RVLGISSGI 
      EDIGGLQWG LFVCLALGW IIIYVIIRK GLHQSGKII 
      WFTALFPYV VLLILLGRA VTLNGASDG LLYFIMPRW 
      EKLLTPGPW MDGATQIFF AYSIGCGAL PALGSYNKF 
      HHNCYKDSL ITCVINTLT SLLAGVVTF SILGYIAAE 
      QNVDVGHVV KTGPGLVFL TYPEVVLKI PGAPFWAAT 
      FFIMLVILG IDSEFCLVE AFITGIVDN WSEQLRPHR 
      SQLTVGVCL LMFLLGIPM VTNGGMYIF QLMDYYSAS 
      GISLLWVCF FQSLAISWI FGVDKISDC IKQMVGVQP 
      NKYWTICWK YCGPLIMIA IFTGHCIEY TNVTYDTYK 
      YPKWAEYIG FGLSISSMV WIPLYAIYY ILSGPGTIL 
      ENLKSGLQP RIKQRKITV CDKRSGLPI TESNIGLIS 
      PSQSII
    CDS sequence
    • >TC015882 RA
      atgtcaaac gaatggggc agccaagtt gaagccatg 
      ccgtccgaa aaagaccta tttattaaa attacgagc 
      aaagccctt gaaaacaat atgcaggaa aggaagttg 
      gtcacgtat aaacaagcc caagataac gaaggggac 
      agaggcggc tggggaaat aaactcgac tttcttttt 
      tcttgcatt agcttatct gtcggtttg ggaaacgta 
      tggagattt ccttatttg tgttacaaa aatggagga 
      ggagctttt atagtgact tacattatc gcaatgatt 
      gtgtgtggg attcccatg ttttttcaa gaagtggcg 
      attggacag tacttagga tcgggagga atgactttt 
      gtaggacaa ctttgtcct atactgaag ggcgtaggc 
      tatgcagcc atgaccata gtgttccta ttagacgtt 
      tattattgt attattata tcatggacg attttttat 
      ttaatgagt atgttttcg tatttgcca gttttgcca 
      tgggctggt tgtgataac tggtggaac accgcagat 
      tgtttttct tctgtaaat aattcaaca tcaaacttc 
      aactcaagc aagttttcc actcccgtt gaagaatat 
      tttgaacgt cgggttctt gggatttct tctggaata 
      gaagatatt ggtggatta cagtggggt ctatttgtt 
      tgtttagcg cttgggtgg ataataatt tacgttatc 
      ataagaaag gggttgcat caaagtgga aagataatt 
      tggtttacg gcactattt ccttatgtc gttctcttg 
      attcttctg ggacgtgct gtaactcta aatggagcc 
      agtgatgga cttctttat tttataatg cccagatgg 
      gagaaacta ctaacacct gggccttgg atggatggt 
      gccacacaa atatttttt gcatatagt ataggctgc 
      ggagccttg ccagcttta ggctcctat aacaagttc 
      catcataac tgttacaaa gattctttg attacatgc 
      gtcataaac actctgact tccttatta gctggagtt 
      gtaacattt tcgatcctt ggttacatt gctgccgaa 
      caaaacgta gatgtggga catgtcgtc aaaactggt 
      ccaggatta gtttttctt acgtatcct gaagtggtt 
      ctgaaaata cctggtgca cctttttgg gccgctact 
      ttttttatt atgcttgtg attttgggt attgacagc 
      gaattttgc ttagttgaa gctttcatc actggaatt 
      gttgacaat tggtcggaa caactaaga ccgcatcga 
      agtcaactc actgtgggt gtttgcttg ttgatgttt 
      ttgcttgga attccaatg gtgaccaac ggcgggatg 
      tatattttc caattgatg gactattac tctgcaagt 
      ggaatatct ctgttatgg gtgtgtttt ttccaatca 
      ctggcgatt tcatggatt tttggagtt gataaaatc 
      agtgattgc atcaaacag atggtgggg gtccaacct 
      aataagtac tggaccatt tgttggaaa tattgcggg 
      cctctaatt atgattgca atttttaca ggacattgc 
      attgaatat acgaacgtt acttacgac acttacaaa 
      tatccaaaa tgggccgaa tatattgga tttggttta 
      agcatatcg tcaatggtt tggatacct ttgtatgcc 
      atatattac atactctca ggtccagga actatactt 
      gagaattta aaatcaggg ttacaacca aggatcaaa 
      caaagaaaa ataacagtt tgtgacaag agatctggt 
      cttcctatt actgagagc aatatagga ctcatttcg 
      cctagtcag tcgattatt taa
    mRNA sequence
    • >TC015882 RA
      agtagtttg attcaaatt ggtagaaat taagtttaa 
      atgtaagaa atttttagt aatgtcaaa cgaatgggg 
      cagccaagt tgaagccat gccgtccga aaaagacct 
      atttattaa aattacgag caaagccct tgaaaacaa 
      tatgcagga aaggaagtt ggtcacgta taaacaagc 
      ccaagataa cgaagggga cagaggcgg ctggggaaa 
      taaactcga ctttctttt ttcttgcat tagcttatc 
      tgtcggttt gggaaacgt atggagatt tccttattt 
      gtgttacaa aaatggagg aggagcttt tatagtgac 
      ttacattat cgcaatgat tgtgtgtgg gattcccat 
      gttttttca agaagtggc gattggaca gtacttagg 
      atcgggagg aatgacttt tgtaggaca actttgtcc 
      tatactgaa gggcgtagg ctatgcagc catgaccat 
      agtgttcct attagacgt ttattattg tattattat 
      atcatggac gatttttta tttaatgag tatgttttc 
      gtatttgcc agttttgcc atgggctgg ttgtgataa 
      ctggtggaa caccgcaga ttgtttttc ttctgtaaa 
      taattcaac atcaaactt caactcaag caagttttc 
      cactcccgt tgaagaata ttttgaacg tcgggttct 
      tgggatttc ttctggaat agaagatat tggtggatt 
      acagtgggg tctatttgt ttgtttagc gcttgggtg 
      gataataat ttacgttat cataagaaa ggggttgca 
      tcaaagtgg aaagataat ttggtttac ggcactatt 
      tccttatgt cgttctctt gattcttct gggacgtgc 
      tgtaactct aaatggagc cagtgatgg acttcttta 
      ttttataat gcccagatg ggagaaact actaacacc 
      tgggccttg gatggatgg tgccacaca aatattttt 
      tgcatatag tataggctg cggagcctt gccagcttt 
      aggctccta taacaagtt ccatcataa ctgttacaa 
      agattcttt gattacatg cgtcataaa cactctgac 
      ttccttatt agctggagt tgtaacatt ttcgatcct 
      tggttacat tgctgccga acaaaacgt agatgtggg 
      acatgtcgt caaaactgg tccaggatt agtttttct 
      tacgtatcc tgaagtggt tctgaaaat acctggtgc 
      acctttttg ggccgctac tttttttat tatgcttgt 
      gattttggg tattgacag cgaattttg cttagttga 
      agctttcat cactggaat tgttgacaa ttggtcgga 
      acaactaag accgcatcg aagtcaact cactgtggg 
      tgtttgctt gttgatgtt tttgcttgg aattccaat 
      ggtgaccaa cggcgggat gtatatttt ccaattgat 
      ggactatta ctctgcaag tggaatatc tctgttatg 
      ggtgtgttt tttccaatc actggcgat ttcatggat 
      ttttggagt tgataaaat cagtgattg catcaaaca 
      gatggtggg ggtccaacc taataagta ctggaccat 
      ttgttggaa atattgcgg gcctctaat tatgattgc 
      aatttttac aggacattg cattgaata tacgaacgt 
      tacttacga cacttacaa atatccaaa atgggccga 
      atatattgg atttggttt aagcatatc gtcaatggt 
      ttggatacc tttgtatgc catatatta catactctc 
      aggtccagg aactatact tgagaattt aaaatcagg 
      gttacaacc aaggatcaa acaaagaaa aataacagt 
      ttgtgacaa gagatctgg tcttcctat tactgagag 
      caatatagg actcatttc gcctagtca gtcgattat 
      ttaaacttt ctgcacttt tgttacatt aaattaaac 
      aaagaatac taaatatgg tcttaaaat tattgtttt 
      gaatacaca aataagaaa ctatgtaca atctacact 
      aaaaaaaaa caaaacaat ttttgaaag aggatcaaa 
      attgttggt aaggtacgt atattacgt acctaccag 
      atgtattaa ataacatgt attaccaag acaaataaa 
      taagtatta atttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY