Status
Please wait ...
Gene information
General information
TC015880 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 422579..439015
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015880
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05919

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015880 RA
      MLRVTIVMG MMVAVVAVL YLTPLPEGT FLSCDRPCH 
      DLDWPMICR IKIQIENKR PCKDCSFNN DTSSEIIAV 
      NGQSPGPAI QICQNDILV VDVVNKMPG HSLTIHWRG 
      QANVEAPFM DGVPLVTQC PIFSYTTFQ YKFRVTSPG 
      THLYQAFSD SELNRGLFG ALIVRQAEK NDLQRKYYD 
      VDSRNHIIM ISERDSKVL VNGKGSSEV TKFTVKRNK 
      RYRFRVAFA GSNSGCPVT LTVDNHLIK VIALDGNLV 
      FPTEVTSVV LTKGERLDF VLKTDQKIK RHYVRVKSC 
      LGEGLALLN YELGDTTPT EKPQKREKE GRVLDTSLC 
      HSQIGKVCL SDLNFVEKM PETVKTAPK TVYLSLGSQ 
      IVNVSGNFG SRVFGVNNL TFTYPSSPL LTQLDRVAM 
      NSVCDGRNV PEKCVGKEV CECVHVEHI PLRAVAEIV 
      LINQDASQE EHIFHLHGY RFYVVGFRH FENAPSTDE 
      IKLLDQENT LFQRNFNNP AIKDTIRIP KNSVVALRF 
      LADNPGFWM LRDEGSRGW TRGLDIVLQ VGEPSDMVS 
      TPTDFPTCG NYIGPDFFL M
    CDS sequence
    • >TC015880 RA
      atgttgaga gtgaccatc gtgatgggc atgatggtg 
      gcagtggtg gcggttttg taccttacc cctttacca 
      gagggcaca ttcctgagc tgtgataga ccttgccat 
      gacctagac tggccaatg atatgtcgc ataaaaatc 
      caaatcgag aataaaagg ccatgtaaa gactgttcc 
      tttaataac gacacatcg tccgaaata atcgcagta 
      aatggccaa agtccaggt ccagcaatt caaatatgc 
      caaaacgat attttagta gtggacgta gttaataaa 
      atgcctgga catagctta acaatacac tggagaggg 
      caagcaaat gttgaagca cctttcatg gacggagtt 
      cctttagtc acccaatgt ccaatattt agctatacc 
      acgtttcag tacaaattt cgggtcact tcccctgga 
      acacatctc tatcaggca ttttcggac agtgagctc 
      aatcggggt ttgtttggt gctcttatc gtaagacaa 
      gctgaaaaa aacgatttg caacggaaa tattacgat 
      gtggattca agaaatcac ataattatg atttctgaa 
      agggatagt aaagtacta gtcaacgga aaaggatca 
      agtgaagtg acaaaattc acagttaag aggaacaaa 
      cgttacagg tttagagta gcatttgct ggaagtaac 
      tcaggatgt cctgttact ttaactgtt gataaccac 
      ctaatcaaa gtaattgca ttggatggt aaccttgtg 
      tttccaacc gaagtaact tccgtggtt ttgaccaaa 
      ggagaaaga ctcgatttt gtgctaaaa accgaccaa 
      aaaatcaaa aggcattac gttagggtt aaatcatgc 
      cttggggaa ggtttagct ttgttaaat tacgaactt 
      ggggacaca acacctact gaaaaaccc caaaaaagg 
      gaaaaagaa gggagggtt ttggacact tctctttgt 
      cactcccaa attggcaaa gtttgttta agcgatttg 
      aattttgtt gaaaaaatg ccagaaaca gttaaaacc 
      gctccaaag accgtgtat ttgtctctc ggatcgcag 
      atcgtaaat gttagtggt aatttcggc tcgagagtt 
      tttggtgtc aataattta acgttcact tatccttcg 
      tctcctttg ttaacgcaa ttggatcga gtcgcgatg 
      aacagcgtt tgtgatggg agaaatgtg cccgaaaaa 
      tgcgtgggg aaggaagtg tgtgaatgt gttcatgtt 
      gagcacatt ccgttgcga gcggttgcc gaaattgtg 
      cttattaac caagatgcg agccaagag gagcacata 
      ttccacttg cacgggtat cgcttttat gtggtcggt 
      tttcgccac tttgaaaac gcaccttca acagacgaa 
      ataaaattg ttggatcaa gaaaacacg ttatttcaa 
      agaaatttc aacaacccc gccataaaa gacactatt 
      cgcattcca aaaaacagc gttgtcgct ttaaggttc 
      ttggcagac aatcctggg ttttggatg ctgagggat 
      gagggatca agaggttgg acaagaggg ttagatatc 
      gttttacaa gttggtgag cccagtgat atggtttcc 
      acgcctacc gattttcca acttgtgga aattacatc 
      ggtccggat ttctttctg atgtaa
    mRNA sequence
    • >TC015880 RA
      atgcagaat taaaattaa aaaaaaata aaacagaat 
      taaattctt ttcattttg gtggagtag agaagcagt 
      ttctttgct acacctgca gtttgcagg tggtctgtt 
      tatttgttt ttattatct cagtgtttg ttatgattt 
      gtgttcatt tttgtggac gttattcag tgtcatcat 
      gaaaccaaa acaataaca ttgttaagc aaggaagat 
      tattaattt tattttatt tgttgagtt ttaaatact 
      agttgtgtt gtaatgtcg tgctgccaa aaatcgagt 
      tataaaatg tccaagaca caaaatgtg atgttgaga 
      gtgaccatc gtgatgggc atgatggtg gcagtggtg 
      gcggttttg taccttacc cctttacca gagggcaca 
      ttcctgagc tgtgataga ccttgccat gacctagac 
      tggccaatg atatgtcgc ataaaaatc caaatcgag 
      aataaaagg ccatgtaaa gactgttcc tttaataac 
      gacacatcg tccgaaata atcgcagta aatggccaa 
      agtccaggt ccagcaatt caaatatgc caaaacgat 
      attttagta gtggacgta gttaataaa atgcctgga 
      catagctta acaatacac tggagaggg caagcaaat 
      gttgaagca cctttcatg gacggagtt cctttagtc 
      acccaatgt ccaatattt agctatacc acgtttcag 
      tacaaattt cgggtcact tcccctgga acacatctc 
      tatcaggca ttttcggac agtgagctc aatcggggt 
      ttgtttggt gctcttatc gtaagacaa gctgaaaaa 
      aacgatttg caacggaaa tattacgat gtggattca 
      agaaatcac ataattatg atttctgaa agggatagt 
      aaagtacta gtcaacgga aaaggatca agtgaagtg 
      acaaaattc acagttaag aggaacaaa cgttacagg 
      tttagagta gcatttgct ggaagtaac tcaggatgt 
      cctgttact ttaactgtt gataaccac ctaatcaaa 
      gtaattgca ttggatggt aaccttgtg tttccaacc 
      gaagtaact tccgtggtt ttgaccaaa ggagaaaga 
      ctcgatttt gtgctaaaa accgaccaa aaaatcaaa 
      aggcattac gttagggtt aaatcatgc cttggggaa 
      ggtttagct ttgttaaat tacgaactt ggggacaca 
      acacctact gaaaaaccc caaaaaagg gaaaaagaa 
      gggagggtt ttggacact tctctttgt cactcccaa 
      attggcaaa gtttgttta agcgatttg aattttgtt 
      gaaaaaatg ccagaaaca gttaaaacc gctccaaag 
      accgtgtat ttgtctctc ggatcgcag atcgtaaat 
      gttagtggt aatttcggc tcgagagtt tttggtgtc 
      aataattta acgttcact tatccttcg tctcctttg 
      ttaacgcaa ttggatcga gtcgcgatg aacagcgtt 
      tgtgatggg agaaatgtg cccgaaaaa tgcgtgggg 
      aaggaagtg tgtgaatgt gttcatgtt gagcacatt 
      ccgttgcga gcggttgcc gaaattgtg cttattaac 
      caagatgcg agccaagag gagcacata ttccacttg 
      cacgggtat cgcttttat gtggtcggt tttcgccac 
      tttgaaaac gcaccttca acagacgaa ataaaattg 
      ttggatcaa gaaaacacg ttatttcaa agaaatttc 
      aacaacccc gccataaaa gacactatt cgcattcca 
      aaaaacagc gttgtcgct ttaaggttc ttggcagac 
      aatcctggg ttttggatg ctgagggat gagggatca 
      agaggttgg acaagaggg ttagatatc gttttacaa 
      gttggtgag cccagtgat atggtttcc acgcctacc 
      gattttcca acttgtgga aattacatc ggtccggat 
      ttctttctg atgtaagac tgtttttac aaacaatta 
      tgttgtaaa acgtgatac gtgataatt acgaaaggg 
      tttatattt ttcctaact caagtcatt gtacagtta 
      cagtaggtt agatttata tttgtgtaa taaaaattc 
      gaaaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY