| General information | |||
| TC015879 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 442248..457425 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015879 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05918 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015879 RA
MSLFKIRTF WSVHCEEDS SYDQNSLLV TKLNHESDF LIVGSHSGI LKVFKPSSD NFKEYTPAD LLIETILKE PILQLSCGR FLSGSSKVQ LAVLQPKSL NVYDLIVKE GATEHGVQN ILEQLYVHK LQKLAFNFC VGPFGGVEN RDFICVQSL DGLLTFFEQ ESFAFCCFL PDVLLPGPL TFVKSSDSF VTSNSNWHI ISFRYKLLS EAGQNSTEG GNVSKFSSD WSFNLGEGI INLDVVQDT TSRITFIMV LGERSIFCL SDIGKLKYM KRLEFSPLN FINYFLNDR IMSLVVSET NNLLIYQNT SLKWCSQLH VSPISFNRG FVCNTNGVL VLLQEDGTL SCSYLGTEP SLFVAPPLA LQDLDFEKA GQELTKLHK IVKNTYSND IKVTNASTE RELVLNVSV SPNLEENTL HKAINNKIC PISIDIMPH TLFEEVQVT VVVDYPLKV SPQTEFFHN LSEKTTMTC CVFMEKPEE VSSLNVQVV ASFISNLGV PRSVMKCAM LPLNLVLET CAPCKESEC KVTLNINQS PVGLSALFP EYILSSNNN AIAFKNTAT DGKVVTVLL AKSSERYRL QSDSFASLN LLVEEMVIR LKKHYSNVD GFSITSNSF PPVNTIVDC IKTHFYAQQ KFVSLQNQI TQLGAQLRL IQKRLIAKF KIKNPTSLA NLELLLGDT YSEIMKLTE EFDEEKNNL VKSRNKLSS VLQLLINIT KLMDVNNKA RQLIESAFN PVIYDLDGQ SWEDITDAS FSYLLRTVL AKSEKDKLR PAQTNFEEV KDVSKIEKH LTLILERIP KGMLQESPS LLDEAEQEN VDTEDNNVT ESLPIGSKI GESSSQLLL SRRGLTKKS
- >TC015879 RA
atgtcgctg tttaaaata cgaacattt tggagtgtt cattgtgag gaggattca tcctatgac caaaatagc ttactagta acgaaactt aatcacgag tctgatttt ttaatagtg ggaagtcat agtggaatt ttgaaagtt tttaaaccc tcaagtgat aattttaaa gaatataca ccggctgat ttactgatt gagacgatt ttaaaagaa ccaattctc caattaagt tgtggtcga tttttatcc ggttcgagc aaagtccag ttagcagtc ttacaaccc aaatcacta aacgtttac gacctaatt gtaaaagaa ggagcaaca gagcatggc gttcaaaat attctggag caactatac gttcataaa ttgcaaaag ctagcgttt aatttctgt gtaggtcct tttggagga gtagaaaac cgtgatttt atttgcgtg caatcgttg gacggtttg ttgactttt tttgaacaa gaaagtttt gcgttttgc tgttttttg ccagatgtt ttgctacca ggtccgtta acttttgtt aaatcgtct gatagtttc gttacatcg aactcgaat tggcacata atcagcttt agatataaa ttgttgtcg gaagccggc caaaattca acagaaggc ggaaacgtg agcaaattc tcaagtgat tggtcattt aatcttggg gaaggaata ataaaccta gatgtggtc caagatact acatccaga ataaccttc attatggta ttgggtgaa agaagcatt ttctgttta agtgacatt ggaaaactt aaatacatg aaacgcctt gaattttca ccactcaat tttatcaac tactttctc aatgacagg attatgtcg cttgtagtt tccgaaaca aataatttg ctgatttat cagaacacg tctttgaaa tggtgctcc cagttgcat gtttctcca atttctttt aatcgtggg tttgtttgc aatacgaac ggtgtcttg gtgttacta caagaggac ggaactctt tcttgttcc tatttaggg actgaaccc agtttgttt gtggcacct cctttggct cttcaagat ttggatttc gaaaaagct ggtcaagaa ttgactaaa ctgcacaaa atcgttaaa aatacatat tccaatgat ataaaagtg accaacgcc tcaactgaa cgcgaacta gtcctaaat gtttcagtg agtccaaat ttagaagaa aacacactt cacaaggca ataaacaat aaaatttgc ccaatttca atagatatc atgcctcat acgttattt gaagaagtg caagtaaca gttgtcgtt gactatcct ttgaaagtt tctccccaa actgagttt tttcataat ttatctgag aaaacgacg atgacttgc tgtgttttt atggagaaa cctgaagag gtctccagt ttgaatgtg caagttgtg gcttcgttt atatcaaat ttaggagtt cctaggagt gtgatgaag tgtgcgatg ttgcctttg aatttagta ctagagaca tgtgcacct tgtaaagaa agcgagtgc aaagtgact ttaaatatt aatcaaagt ccggtgggt ttatctgcg ctttttcca gaatacata ctttcttcg aacaacaac gcaattgcg tttaaaaac acagcaaca gatggaaaa gttgttaca gttttgtta gcaaaatcg tctgaaagg tacagactt caatcagac tcattcgca tcacttaat ctcttggtg gaagaaatg gtgattcgg ctaaaaaaa cactattcc aatgtcgat ggtttttct atcacttct aattctttc ccaccagtt aatacgata gttgattgc attaaaaca catttttat gcgcaacag aaatttgtt tcgttacag aaccagatt acacaactt ggtgcccaa ttaagatta atccagaag cgactaatt gcaaagttt aaaatcaaa aatccaact tctttggcc aatttggag ctacttctt ggagacact tattcggaa attatgaaa ctcaccgaa gaatttgat gaagaaaaa aataattta gtgaagagt agaaataaa ttgtcgtcg gttttgcaa cttttaatt aatatcacg aaattaatg gatgttaat aataaagcg agacagttg attgagtct gcttttaac cccgttatc tacgatctt gatggacaa agttgggaa gacataact gacgcatct ttttcctat ttattgcgc actgttttg gcaaaatct gagaaagat aaattacga cctgctcaa accaacttc gaggaagtc aaggatgtg tcaaaaatc gaaaaacat ctaactcta atacttgag agaattcca aaaggaatg ttacaggaa tctcctagt ttacttgat gaagctgaa caagaaaat gtggacact gaagacaac aatgtgacg gaaagtttg ccgattggg tccaaaatt ggagaatcc agttctcaa ctgctttta tcgaggaga ggtcttacg aaaaagtcg tag
- >TC015879 RA
gtaacgaag tcggtccat gttttgatt tccgtgcga tgggcatca taccatttt cttgccgct cccacaaga tggttcctg gctgtctcc aagcaacat tcgtttgac cacgaatgg atttctgcc aagaacttt ctgatcgct ttgtataat aacactgga ggaaagcct tttccctta ttccggagg agaatagag ttttttcta tgttagaat acaaacacc tattataca acagggtga tcttataac accttgtat tattccaat ctgatttgt tgggagaaa aatcgttta tttcaaatg tttgaaact ctaccttcc ctctgagct tttttttcg ttttttttt tgtagaatt ctttaaaat atctattta ggataccgc acattgatc gccttgtgg cataagact attgtcgtt gcatggtaa agatctaag tatttctaa tatagttct aatctatgg tttagacag aacaacttg tataaagta acgtgaccg tacaaaaaa atttatcaa tttatttat tgttttttt aaattattt acgaaactt atttatgtt ttttccaag ttatcaact tcgtttctt ttttatttc aaactttat tttgtacag ttgcgttat tttataaac tagcgagtt aaagtatga ggagacaaa acttagcta tttaaggta aatctttta ctccctcta gcgattgaa tttgaactg ctccttgtg catgtcacg gcactcaac agcttctta attaatttt agttaaaaa tataaaaat tataataag atagctgtt gcattaaat tttatttat ttgtgtata tcagttgta gattttaac tagtcatta caagaaata tggttaaca gtaataaaa ttgttatta attttgttt ttttttgtg ttcagagtt ttgtattta tgtaggact attgtaacc aaagtattt aaataattc tcttccaag gaataaatg aatatattt taaagcatt tgtatttat tgattttcc ttgtttaca ttcaccgta atcaatata tttctaacc gctagttcc tataaaaca gtgagtgaa catgtcgct gtttaaaat acgaacatt ttggagtgt tcattgtga ggaggattc atcctatga ccaaaatag cttactagt aacgaaact taatcacga gtctgattt tttaatagt gggaagtca tagtggaat tttgaaagt ttttaaacc ctcaagtga taattttaa agaatatac accggctga tttactgat tgagacgat tttaaaaga accaattct ccaattaag ttgtggtcg atttttatc cggttcgag caaagtcca gttagcagt cttacaacc caaatcact aaacgttta cgacctaat tgtaaaaga aggagcaac agagcatgg cgttcaaaa tattctgga gcaactata cgttcataa attgcaaaa gctagcgtt taatttctg tgtaggtcc ttttggagg agtagaaaa ccgtgattt tatttgcgt gcaatcgtt ggacggttt gttgacttt ttttgaaca agaaagttt tgcgttttg ctgtttttt gccagatgt tttgctacc aggtccgtt aacttttgt taaatcgtc tgatagttt cgttacatc gaactcgaa ttggcacat aatcagctt tagatataa attgttgtc ggaagccgg ccaaaattc aacagaagg cggaaacgt gagcaaatt ctcaagtga ttggtcatt taatcttgg ggaaggaat aataaacct agatgtggt ccaagatac tacatccag aataacctt cattatggt attgggtga aagaagcat tttctgttt aagtgacat tggaaaact taaatacat gaaacgcct tgaattttc accactcaa ttttatcaa ctactttct caatgacag gattatgtc gcttgtagt ttccgaaac aaataattt gctgattta tcagaacac gtctttgaa atggtgctc ccagttgca tgtttctcc aatttcttt taatcgtgg gtttgtttg caatacgaa cggtgtctt ggtgttact acaagagga cggaactct ttcttgttc ctatttagg gactgaacc cagtttgtt tgtggcacc tcctttggc tcttcaaga tttggattt cgaaaaagc tggtcaaga attgactaa actgcacaa aatcgttaa aaatacata ttccaatga tataaaagt gaccaacgc ctcaactga acgcgaact agtcctaaa tgtttcagt gagtccaaa tttagaaga aaacacact tcacaaggc aataaacaa taaaatttg cccaatttc aatagatat catgcctca tacgttatt tgaagaagt gcaagtaac agttgtcgt tgactatcc tttgaaagt ttctcccca aactgagtt ttttcataa tttatctga gaaaacgac gatgacttg ctgtgtttt tatggagaa acctgaaga ggtctccag tttgaatgt gcaagttgt ggcttcgtt tatatcaaa tttaggagt tcctaggag tgtgatgaa gtgtgcgat gttgccttt gaatttagt actagagac atgtgcacc ttgtaaaga aagcgagtg caaagtgac tttaaatat taatcaaag tccggtggg tttatctgc gctttttcc agaatacat actttcttc gaacaacaa cgcaattgc gtttaaaaa cacagcaac agatggaaa agttgttac agttttgtt agcaaaatc gtctgaaag gtacagact tcaatcaga ctcattcgc atcacttaa tctcttggt ggaagaaat ggtgattcg gctaaaaaa acactattc caatgtcga tggtttttc tatcacttc taattcttt cccaccagt taatacgat agttgattg cattaaaac acattttta tgcgcaaca gaaatttgt ttcgttaca gaaccagat tacacaact tggtgccca attaagatt aatccagaa gcgactaat tgcaaagtt taaaatcaa aaatccaac ttctttggc caatttgga gctacttct tggagacac ttattcgga aattatgaa actcaccga agaatttga tgaagaaaa aaataattt agtgaagag tagaaataa attgtcgtc ggttttgca acttttaat taatatcac gaaattaat ggatgttaa taataaagc gagacagtt gattgagtc tgcttttaa ccccgttat ctacgatct tgatggaca aagttggga agacataac tgacgcatc tttttccta tttattgcg cactgtttt ggcaaaatc tgagaaaga taaattacg acctgctca aaccaactt cgaggaagt caaggatgt gtcaaaaat cgaaaaaca tctaactct aatacttga gagaattcc aaaaggaat gttacagga atctcctag tttacttga tgaagctga acaagaaaa tgtggacac tgaagacaa caatgtgac ggaaagttt gccgattgg gtccaaaat tggagaatc cagttctca actgctttt atcgaggag aggtcttac gaaaaagtc gtagtgtat accttcagg tgtaaaatt tctctcgga cgtacccac tattgttaa ataaacttt cagtttttt gattgtata gcatagtct cactttcta atgtacgtg taaacagtg ccatttcaa tttatgaaa taagtgtat tgtaagtag gtagcttga agtagaaac ttgtatacg ttgtacaag gaatggaaa gagttaaca attgttttg ctcaaaatt agcagagaa ttttaggca acggataaa gttttagtt taattttaa ttcgtagtt cgtatccta ctgttaaga tgtgaaaga aaacattat acattacag gacagtttt ttacgtata atttaatgg tatttagag ttaggtata tgttttcag tgaactaca gattgttaa agttaaaaa acaaatttt atcgttata cttactttt tgaataaag agaatttac ttga
