Status
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Gene information
General information
TC015854 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9466154..9502201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015854
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05914

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015854 RA
      MGLYKQYFR IGQYARNQI LGKADRSKD GTPLQMLIS 
      QQGKLSSSR IAVILIGVF TLAMGIILS SIPWVDYLI 
      LKNLKLWNG SLSFQYWQK PGVIRLTKV YIFNVTNPD 
      SFLNLGEKP KLQEVGPFV YREDMEKVN IKFHDNGTL 
      TYQHKKILQ FVPELSVDK DQKITVPNI PLLTLSTQS 
      NSLSYFVQR TISFLLSVR GFKPFVTIT ADELVFGYD 
      DTLVSLAHQ FYPKRKRPM SKMGLLINR NGTLNEVHN 
      IYTGMTGMQ NFGYLEKLN GVDKLPYWQ ESPCNIIRA 
      SEGSFFPPR YYTKSDVVN IYDKDLCRT MPLQYRGPV 
      TKHGISADL YTPADSMFE TVMKEPNNK CYCPNNEFC 
      PPKGLQNIS PCQFDAPVY LSFPHFFEA DPTLIEPFE 
      GLNPVKEKH QSYFKIQPR LGVPIEGKV RLQLNLKVD 
      QAPQVAPVS KFPSIMYPI MWLEEGIDD LTPPIRRWI 
      YLATTFADV ACPLMTYGF IFLGTCILI GVFINAYKS 
      LVFTKETIE IGMKTLRKR GSGYLAVAR PKLIKRDTY 
      TLLDLNEGL DEIDI
    CDS sequence
    • >TC015854 RA
      atgggactc tacaagcag tacttccgg atcgggcag 
      tatgccaga aatcaaatt ttgggcaag gccgatcga 
      tccaaggat ggcactcct ttgcaaatg cttatttcg 
      caacaaggg aaactcagc agtagtcgc atcgctgtc 
      atcctaatc ggagtattt accttggcc atgggcatt 
      atcttatcg tcaatccca tgggtggac tacctcatt 
      ttaaaaaat ttgaaattg tggaacggc tcactgagc 
      ttccaatac tggcaaaag cctggagta attcgcctc 
      acaaaagtc tacattttc aacgtcaca aaccctgac 
      agttttcta aatttaggg gaaaaacca aaactgcaa 
      gaagtcggc ccttttgtc tacagagaa gacatggaa 
      aaggtcaat atcaagttc cacgataac ggcacacta 
      acgtatcag cacaaaaaa atcctacaa tttgtacct 
      gaattatcc gttgataag gaccagaag atcactgtt 
      cccaacatt ccgcttctg accttatcg acccaatct 
      aactctctc agttacttc gtccaacga acaatttct 
      ttcctgtta agtgtacga ggtttcaaa cccttcgtc 
      acaataaca gcagatgag ctagtcttc ggctacgac 
      gacactcta gtctcgcta gcccaccag ttttatccg 
      aaacgaaaa cgcccaatg tccaaaatg gggctttta 
      atcaacaga aatggaacc ttgaatgag gtccataac 
      atatataca gggatgacc ggaatgcaa aatttcggc 
      tacttggag aagctcaac ggagtcgac aagttgccc 
      tactggcag gagtcaccc tgtaacatc ataagggcc 
      agcgaaggg tcctttttc ccgcccaga tactatacc 
      aagtccgat gttgtcaat atttatgac aaggatttg 
      tgcagaacc atgcccttg cagtacagg gggccagtc 
      acaaaacat ggaatcagc gctgatttg tacaccccg 
      gctgatagc atgttcgag actgtgatg aaagagccc 
      aacaacaaa tgttactgc cccaacaac gagttttgc 
      cccccaaaa ggtctccaa aacatcagc ccttgtcaa 
      ttcgatgcc ccggtttat ttatcgttc ccgcacttt 
      ttcgaagcc gatccaacg ctaattgag ccttttgaa 
      ggcctgaat ccagtcaaa gagaagcac cagtcctat 
      tttaaaatc caaccgcgt ctgggggtg ccaattgag 
      ggcaaagtg cgtttgcag ctcaatttg aaagtggac 
      caagcaccc caggtggcc cccgtgtcg aaattcccc 
      agtatcatg taccccatc atgtggctt gaggagggt 
      attgatgac ttgacgccc cccatcaga cgctggatt 
      tacctcgca acgactttt gccgatgtt gcgtgcccc 
      cttatgacc tacgggttc atttttcta gggacttgt 
      attttgatc ggtgtgttt ataaacgcg tacaaatca 
      cttgttttt acgaaggaa accatcgag atcgggatg 
      aagacgctg aggaagcga gggagtggt tatttggcc 
      gtggcaagg cctaaactg atcaaaaga gacacgtat 
      acactgctc gatttgaac gaaggattg gacgagatt 
      gatatatga
    mRNA sequence
    • >TC015854 RA
      ccacccatc aaactgtcc cacatataa tttgccccc 
      accgccatc gactgataa ctaacaatt ctcttttcg 
      taatggaga cagtgcgct ctcgccttc ttcgcgacg 
      tttatcctt aaggacgat gtgagtgga gagagagct 
      tcggagctt tcgcgaaca cgcggtgtt taaaaacaa 
      acacaaact taataaaaa aggattccg cgcgcgcgc 
      tatgtttac gtttttgtg gtgaaaagg atacccgat 
      ttgggtacg gcaccatgg gactctaca agcagtact 
      tccggatcg ggcagtatg ccagaaatc aaattttgg 
      gcaaggccg atcgatcca aggatggca ctcctttgc 
      aaatgctta tttcgcaac aagggaaac tcagcagta 
      gtcgcatcg ctgtcatcc taatcggag tatttacct 
      tggccatgg gcattatct tatcgtcaa tcccatggg 
      tggactacc tcattttaa aaaatttga aattgtgga 
      acggctcac tgagcttcc aatactggc aaaagcctg 
      gagtaattc gcctcacaa aagtctaca ttttcaacg 
      tcacaaacc ctgacagtt ttctaaatt taggggaaa 
      aaccaaaac tgcaagaag tcggccctt ttgtctaca 
      gagaagaca tggaaaagg tcaatatca agttccacg 
      ataacggca cactaacgt atcagcaca aaaaaatcc 
      tacaatttg tacctgaat tatccgttg ataaggacc 
      agaagatca ctgttccca acattccgc ttctgacct 
      tatcgaccc aatctaact ctctcagtt acttcgtcc 
      aacgaacaa tttctttcc tgttaagtg tacgaggtt 
      tcaaaccct tcgtcacaa taacagcag atgagctag 
      tcttcggct acgacgaca ctctagtct cgctagccc 
      accagtttt atccgaaac gaaaacgcc caatgtcca 
      aaatggggc ttttaatca acagaaatg gaaccttga 
      atgaggtcc ataacatat atacaggga tgaccggaa 
      tgcaaaatt tcggctact tggagaagc tcaacggag 
      tcgacaagt tgccctact ggcaggagt caccctgta 
      acatcataa gggccagcg aagggtcct ttttcccgc 
      ccagatact ataccaagt ccgatgttg tcaatattt 
      atgacaagg atttgtgca gaaccatgc ccttgcagt 
      acagggggc cagtcacaa aacatggaa tcagcgctg 
      atttgtaca ccccggctg atagcatgt tcgagactg 
      tgatgaaag agcccaaca acaaatgtt actgcccca 
      acaacgagt tttgccccc caaaaggtc tccaaaaca 
      tcagccctt gtcaattcg atgccccgg tttatttat 
      cgttcccgc actttttcg aagccgatc caacgctaa 
      ttgagcctt ttgaaggcc tgaatccag tcaaagaga 
      agcaccagt cctatttta aaatccaac cgcgtctgg 
      gggtgccaa ttgagggca aagtgcgtt tgcagctca 
      atttgaaag tggaccaag caccccagg tggcccccg 
      tgtcgaaat tccccagta tcatgtacc ccatcatgt 
      ggcttgagg agggtattg atgacttga cgcccccca 
      tcagacgct ggatttacc tcgcaacga cttttgccg 
      atgttgcgt gccccctta tgacctacg ggttcattt 
      ttctaggga cttgtattt tgatcggtg tgtttataa 
      acgcgtaca aatcacttg tttttacga aggaaacca 
      tcgagatcg ggatgaaga cgctgagga agcgaggga 
      gtggttatt tggccgtgg caaggccta aactgatca 
      aaagagaca cgtatacac tgctcgatt tgaacgaag 
      gattggacg agattgata tatgagtgc gagttgtag 
      ttctggtca attgtgatt gtagaaaat tgaactctg 
      ccgaattgc tcaaattgt gattcttgt gataatttt 
      tggtgcaat aatttattt tttgaattg ccaaacgtt 
      gactaggtt taggtgtag tggcggatg attgcggga 
      ttgaaattg gtgcctttt atcagatga gtcatcaca 
      caaactttg atttttgct cagttaggt ctaggagag 
      ccagtttta taatgaaat gtacttttt taataaaat 
      acttaaact tgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY