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Gene information
General information
TC015853 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9443094..9452808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015853
    iBeetle screen iB_05913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015853 RA
      MAATKPRII PIPCTSHQH KESQTNIED SLKVFLSTD 
      MEFKVKKEK WRDLAKQLK IAVFVGIWI VCSCALMTK 
      NVKVQHMHQ LSISSGETK SFLIFEEPQ NTHFRVTLE 
      GALLPSCYE NLSVNFLNV WVQLVVLKQ PSRKLKPSN 
      VLLVQNVSD LWEVALVPE KLIDVVPAV THEKTFHLN 
      SIKVGDFNN SLLEVRFST NMKTNFPLS LGYNLEPIN 
      TDLGIIYAG LVLIGLYIA IIFELVHRT LAAMLASTM 
      SVAILAVLN ARPTLAEIV SWIDIETLL LLFSMMTLV 
      TILSETGIF DYMSVLAYK ITGGKIWPL INTLCLTTA 
      IFSCFLDNV TTTLLTTPV TVKLCEVMK LNPVPVLMY 
      MLIFANIGG AITPIGDPP NVIIASNAD VIRSGINFG 
      TFTLHMGVG SIIAFLVVY AQLRYSHRD LKLFKYAEP 
      TEVQELRRE IAVWKRAAA SLSSYSKDE DTVKESLLR 
      RSTTLLGKL KLTANITSS NIENYTTNL KDLQRQYPI 
      RNKVLLIKS GITLSLVIC VFFLHSIPA MSILGLGWT 
      ALLGTLLLL LLYDKEDIE GILGRVEWS TLLFFASLF 
      ILMEALSRL GLIDWVGKQ TQTVIMSVD QDSRLTVAI 
      VLILWVSGI ASAFVDNLP LTTMMIRIA TNLANDQEL 
      QLPLQPLIW ALSFGACLG GNGSLFGSS SNIVCAGVA 
      EQHGYRFTF LQFTKVGLP VTITSMAVI TVYLIVCHV 
      VFCWH
    CDS sequence
    • >TC015853 RA
      atggccgct actaaacca aggataatc ccaattcct 
      tgcacatca caccaacac aaagagtcc caaaccaac 
      atcgaagac agccttaaa gtatttctc tcaacagac 
      atggaattt aaggtcaag aaagagaaa tggcgcgac 
      ctggccaaa caattaaaa atcgccgtt ttcgtgggc 
      atctggatc gtgtgcagt tgtgcccta atgaccaaa 
      aacgtcaaa gttcagcac atgcaccag ttgagcatt 
      tcaagtggc gaaacgaaa agttttttg attttcgag 
      gagccgcaa aatacgcac tttcgggtc actctggaa 
      ggtgccctt cttccgtcg tgttacgag aacttgtcg 
      gtgaatttt ctgaacgtt tgggtgcag ttggtagtc 
      ctgaagcag ccgtcccgg aaactaaaa cccagcaat 
      gttcttctc gtccagaac gtttcggac ctttgggaa 
      gtggctctg gtgccggaa aagctgatt gatgtggtt 
      ccggcagtg acgcatgag aagacgttt catttgaat 
      tcgatcaaa gttggcgat tttaataat agtttattg 
      gaggttcgg tttagtacc aatatgaag acaaatttt 
      cctctatcg ctaggttat aaccttgaa ccgattaac 
      accgacttg gggattatt tacgcaggg ttggtacta 
      attggtttg tatatcgcc ataattttc gagcttgtc 
      caccggacg ttggcagcc atgcttgcg agcactatg 
      tcggttgcg atattggca gtactgaat gccaggcct 
      actttggcc gaaattgtc tcatggatc gacattgag 
      acgctttta ctcctcttt tcaatgatg acacttgtg 
      acaatttta agtgaaaca gggattttt gattatatg 
      tcagtcctg gcatataag attacaggt gggaaaatt 
      tggccgttg atcaatact ttgtgtcta acgacggcg 
      attttttcc tgttttttg gataatgtc acaacgact 
      ttactcact acgcctgtc actgtcaaa ttgtgtgaa 
      gttatgaaa ctaaaccct gttcccgta cttatgtat 
      atgttgatt tttgcaaat atagggggc gctatcacc 
      ccgatcggt gatcccccc aatgtcatt attgcatca 
      aatgctgat gtcatcaga tcgggtatt aattttggg 
      acttttact ctgcatatg ggagtgggg tccataatt 
      gccttcttg gtcgtttat gcacagctg aggtacagc 
      catcgggac cttaagctc ttcaaatat gcggaaccc 
      accgaagta caagaactt cgtcgtgaa atagccgtt 
      tggaaacgt gctgcagct tctttgagt agctatagc 
      aaggatgaa gacacggta aaagagtcc ctcttacgt 
      cgaagtacg actcttttg ggcaaactt aaattaact 
      gccaacata acaagttca aacatcgaa aattacacc 
      acaaacttg aaagatttg caacgccag tacccgata 
      agaaacaaa gtactatta atcaaatcc ggaataacg 
      ctaagtttg gtcatttgt gtcttcttc ttacactca 
      atcccagct atgagtata ctagggcta ggctggacg 
      gcactgctc ggcacctta ctcttactt cttttatat 
      gacaaggag gacattgag gggatttta ggccgagtt 
      gagtggtcc acactacta tttttcgca tcgttgttc 
      atcctgatg gaggccttg tccaggtta ggtttaatt 
      gattgggtg ggcaaacaa acgcaaact gtgatcatg 
      tcagttgat caagactca cgattgaca gttgccata 
      gttttgatt ttgtgggtt tcggggatt gcctcagct 
      tttgtcgac aatctcccc ctgactact atgatgatc 
      cgaatcgct acaaacttg gccaatgat caagaacta 
      caattaccg ttacaaccg ctgatttgg gccctatct 
      tttggagct tgtcttgga ggtaatggt agtttgttt 
      ggttcgtct tcgaatatt gtgtgtgcc ggagtggcc 
      gaacaacat ggctacaga tttacgttt ctgcaattt 
      acaaaggtt ggcctccct gtcacaatc acaagcatg 
      gcggttatc actgtttat ttaattgtg tgtcatgtg 
      gtgttttgt tggcactga
    mRNA sequence
    • >TC015853 RA
      cttgtggca aaaaattat ctttggatt gatagtccg 
      ccatcttcg ccaacgatg cgatcgtgt tcccgtaaa 
      taaaagaat ggccgctac taaaccaag gataatccc 
      aattccttg cacatcaca ccaacacaa agagtccca 
      aaccaacat cgaagacag ccttaaagt atttctctc 
      aacagacat ggaatttaa ggtcaagaa agagaaatg 
      gcgcgacct ggccaaaca attaaaaat cgccgtttt 
      cgtgggcat ctggatcgt gtgcagttg tgccctaat 
      gaccaaaaa cgtcaaagt tcagcacat gcaccagtt 
      gagcatttc aagtggcga aacgaaaag ttttttgat 
      tttcgagga gccgcaaaa tacgcactt tcgggtcac 
      tctggaagg tgcccttct tccgtcgtg ttacgagaa 
      cttgtcggt gaattttct gaacgtttg ggtgcagtt 
      ggtagtcct gaagcagcc gtcccggaa actaaaacc 
      cagcaatgt tcttctcgt ccagaacgt ttcggacct 
      ttgggaagt ggctctggt gccggaaaa gctgattga 
      tgtggttcc ggcagtgac gcatgagaa gacgtttca 
      tttgaattc gatcaaagt tggcgattt taataatag 
      tttattgga ggttcggtt tagtaccaa tatgaagac 
      aaattttcc tctatcgct aggttataa ccttgaacc 
      gattaacac cgacttggg gattattta cgcagggtt 
      ggtactaat tggtttgta tatcgccat aattttcga 
      gcttgtcca ccggacgtt ggcagccat gcttgcgag 
      cactatgtc ggttgcgat attggcagt actgaatgc 
      caggcctac tttggccga aattgtctc atggatcga 
      cattgagac gcttttact cctcttttc aatgatgac 
      acttgtgac aattttaag tgaaacagg gatttttga 
      ttatatgtc agtcctggc atataagat tacaggtgg 
      gaaaatttg gccgttgat caatacttt gtgtctaac 
      gacggcgat tttttcctg ttttttgga taatgtcac 
      aacgacttt actcactac gcctgtcac tgtcaaatt 
      gtgtgaagt tatgaaact aaaccctgt tcccgtact 
      tatgtatat gttgatttt tgcaaatat agggggcgc 
      tatcacccc gatcggtga tccccccaa tgtcattat 
      tgcatcaaa tgctgatgt catcagatc gggtattaa 
      ttttgggac ttttactct gcatatggg agtggggtc 
      cataattgc cttcttggt cgtttatgc acagctgag 
      gtacagcca tcgggacct taagctctt caaatatgc 
      ggaacccac cgaagtaca agaacttcg tcgtgaaat 
      agccgtttg gaaacgtgc tgcagcttc tttgagtag 
      ctatagcaa ggatgaaga cacggtaaa agagtccct 
      cttacgtcg aagtacgac tcttttggg caaacttaa 
      attaactgc caacataac aagttcaaa catcgaaaa 
      ttacaccac aaacttgaa agatttgca acgccagta 
      cccgataag aaacaaagt actattaat caaatccgg 
      aataacgct aagtttggt catttgtgt cttcttctt 
      acactcaat cccagctat gagtatact agggctagg 
      ctggacggc actgctcgg caccttact cttacttct 
      tttatatga caaggagga cattgaggg gattttagg 
      ccgagttga gtggtccac actactatt tttcgcatc 
      gttgttcat cctgatgga ggccttgtc caggttagg 
      tttaattga ttgggtggg caaacaaac gcaaactgt 
      gatcatgtc agttgatca agactcacg attgacagt 
      tgccatagt tttgatttt gtgggtttc ggggattgc 
      ctcagcttt tgtcgacaa tctccccct gactactat 
      gatgatccg aatcgctac aaacttggc caatgatca 
      agaactaca attaccgtt acaaccgct gatttgggc 
      cctatcttt tggagcttg tcttggagg taatggtag 
      tttgtttgg ttcgtcttc gaatattgt gtgtgccgg 
      agtggccga acaacatgg ctacagatt tacgtttct 
      gcaatttac aaaggttgg cctccctgt cacaatcac 
      aagcatggc ggttatcac tgtttattt aattgtgtg 
      tcatgtggt gttttgttg gcactgaat cgtgttttt 
      caatcactc tgtatcttg ttaacattt attattgtt 
      ataaatttc taatataaa aatgtaatt att
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