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Gene information
General information
TC015852 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9432264..9439971
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015852
    iBeetle screen iB_05912

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015852 RA
      MTRNSSYSF KQFNDEITD LYKSLKDNN YTDDEIRKI 
      FAPLAPKTP NKLSTALIL TSLATLALL YFATYFETI 
      SWHLSALGR IFLIKLLPL WDWTSLKNE KCLIWKPST 
      TTTPEITNF NCALCESIQ VVDVYDEIS QETLKNRYL 
      DVDVPVIIR QSWPKMADF FETLVKTAA IADSHPCSL 
      ATNIYNGVT SVKNLVSKT QLFDRFFLH FQNCEFGAV 
      KQFRGFTPR PAFLPPELS PVQYNWLLW SRDYNMSQF 
      KAIGLVDKV AVVGQIAGA NFIRLVPRG NCEGECPQI 
      GVKLQEGES IVLAGLWNL EYRSEVGEN LAAILEMH
    CDS sequence
    • >TC015852 RA
      atgaccagg aactcctcc tactccttc aaacaattc 
      aacgacgaa atcaccgac ttgtacaag tccctcaaa 
      gacaacaac tacaccgac gatgagatt cgcaagatt 
      ttcgcgcct ttggccccc aaaacccca aacaaactt 
      tcaacagcc cttatcctc acttcacta gccactttg 
      gcccttctg tacttcgcc acttacttt gaaaccatc 
      tcgtggcac ttatcagcc cttggacgc attttcctt 
      attaaattg ctgccactt tgggactgg actagccta 
      aaaaacgaa aaatgcctc atctggaaa ccatcaacc 
      acaacaacc cccgaaata accaacttc aactgcgct 
      ttatgcgaa agcatccaa gttgttgac gtttacgac 
      gaaatctcg caagaaact ttgaaaaat cgttacttg 
      gacgtggac gttccagtg ataatcagg caaagttgg 
      cccaaaatg gccgacttt ttcgaaaca cttgtcaaa 
      accgctgcc attgctgac tcacaccct tgcagtttg 
      gcaacaaac atttacaac ggagtgacg agtgttaaa 
      aacttggtt tcgaagacg caactgttc gatagattt 
      tttctccat tttcaaaat tgcgaattt ggcgccgtt 
      aagcaattt cgggggttt accccacgc ccggcgttt 
      ctcccacct gaactatca ccggtgcaa tataattgg 
      ctgttgtgg agtcgggat tacaacatg agtcagttt 
      aaagccatc gggttggtc gataaagtg gcagtggtg 
      gggcaaatt gccggcgca aatttcata cgattggtt 
      ccacggggg aattgcgaa ggggagtgc ccccaaatt 
      ggcgttaaa ctacaggag ggggagtcg attgttttg 
      gcgggatta tggaatttg gagtatcgg tcagaggtg 
      ggggaaaat ctcgccgcc attttggaa atgcattag
    mRNA sequence
    • >TC015852 RA
      tttcaccaa agggctttt tgcacgaga agatgacca 
      ggaactcct cctactcct tcaaacaat tcaacgacg 
      aaatcaccg acttgtaca agtccctca aagacaaca 
      actacaccg acgatgaga ttcgcaaga ttttcgcgc 
      ctttggccc ccaaaaccc caaacaaac tttcaacag 
      cccttatcc tcacttcac tagccactt tggcccttc 
      tgtacttcg ccacttact ttgaaacca tctcgtggc 
      acttatcag cccttggac gcattttcc ttattaaat 
      tgctgccac tttgggact ggactagcc taaaaaacg 
      aaaaatgcc tcatctgga aaccatcaa ccacaacaa 
      cccccgaaa taaccaact tcaactgcg ctttatgcg 
      aaagcatcc aagttgttg acgtttacg acgaaatct 
      cgcaagaaa ctttgaaaa atcgttact tggacgtgg 
      acgttccag tgataatca ggcaaagtt ggcccaaaa 
      tggccgact ttttcgaaa cacttgtca aaaccgctg 
      ccattgctg actcacacc cttgcagtt tggcaacaa 
      acatttaca acggagtga cgagtgtta aaaacttgg 
      tttcgaaga cgcaactgt tcgatagat tttttctcc 
      attttcaaa attgcgaat ttggcgccg ttaagcaat 
      ttcgggggt ttaccccac gcccggcgt ttctcccac 
      ctgaactat caccggtgc aatataatt ggctgttgt 
      ggagtcggg attacaaca tgagtcagt ttaaagcca 
      tcgggttgg tcgataaag tggcagtgg tggggcaaa 
      ttgccggcg caaatttca tacgattgg ttccacggg 
      ggaattgcg aaggggagt gcccccaaa ttggcgtta 
      aactacagg agggggagt cgattgttt tggcgggat 
      tatggaatt tggagtatc ggtcagagg tgggggaaa 
      atctcgccg ccattttgg aaatgcatt agtatcatg 
      taatatgtg ggaataata aaagacaaa tacagca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY