Status
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Gene information
General information
TC015844 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9279888..9281209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015844
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05911

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015844 RA
      MKTLLIVFV VALKCQAVK FPHTFKLCK RSDPDFPKC 
      LREAAEFNI HQLVHPFKD LRIPGLKPL LIPSLVIGS 
      GKRAVAVEQ RFHNCNFHG FEHVLLEKF EFDFKKNTL 
      IIDSHIPEV TKKCDYKLS GRVLLLPIQ GEGESTIVL 
      KNVTIAGYF NYETVKRSG KTYLRFVDH HLSLDVSHV 
      YYHFDNLFN GDKALGDNI NKVLNDNWS EVFEDVKGG 
      YSEVMNQII QTLLNNFWS VVSLEDAFG EK
    CDS sequence
    • >TC015844 RA
      atgaagacg ttgttaatt gtttttgtg gtggccctc 
      aagtgccaa gctgtgaaa tttccccac actttcaaa 
      ctgtgcaaa cgctcagat ccggacttt cccaagtgt 
      ttgagggaa gcagctgag tttaatatt caccagcta 
      gttcacccg ttcaaagat ctgagaata ccggggctc 
      aaaccctta ctgattccg tcactggtg attgggtcg 
      ggcaaaagg gccgtcgcc gtcgagcaa cgtttccat 
      aattgtaat tttcacgga tttgaacac gttttattg 
      gaaaaattt gaatttgat tttaagaaa aatacgcta 
      attatcgac agccacatt cctgaagtt acgaaaaag 
      tgtgattat aagttaagt ggcagggtt ttgcttttg 
      ccgatacag ggtgaaggg gaaagtacc attgtgtta 
      aaaaacgta acgattgcg ggatatttc aattacgag 
      actgtgaaa cgaagtgga aaaacatat ttgaggttc 
      gttgaccat cacttatca ctagacgtc agccatgtt 
      tactaccat ttcgataat ctcttcaac ggtgataag 
      gccctgggt gataacatc aacaaagtg ttgaatgat 
      aactggagt gaagtcttc gaagatgtg aaaggtgga 
      tattcggaa gtcatgaac cagataatt caaacgctg 
      ttgaataat ttttggtcg gtggtctcc cttgaggat 
      gctttcggc gaaaaatag
    mRNA sequence
    • >TC015844 RA
      tgcaaacaa catgaagac gttgttaat tgtttttgt 
      ggtggccct caagtgcca agctgtgaa atttcccca 
      cactttcaa actgtgcaa acgctcaga tccggactt 
      tcccaagtg tttgaggga agcagctga gtttaatat 
      tcaccagct agttcaccc gttcaaaga tctgagaat 
      accggggct caaaccctt actgattcc gtcactggt 
      gattgggtc gggcaaaag ggccgtcgc cgtcgagca 
      acgtttcca taattgtaa ttttcacgg atttgaaca 
      cgttttatt ggaaaaatt tgaatttga ttttaagaa 
      aaatacgct aattatcga cagccacat tcctgaagt 
      tacgaaaaa gtgtgatta taagttaag tggcagggt 
      tttgctttt gccgataca gggtgaagg ggaaagtac 
      cattgtgtt aaaaaacgt aacgattgc gggatattt 
      caattacga gactgtgaa acgaagtgg aaaaacata 
      tttgaggtt cgttgacca tcacttatc actagacgt 
      cagccatgt ttactacca tttcgataa tctcttcaa 
      cggtgataa ggccctggg tgataacat caacaaagt 
      gttgaatga taactggag tgaagtctt cgaagatgt 
      gaaaggtgg atattcgga agtcatgaa ccagataat 
      tcaaacgct gttgaataa tttttggtc ggtggtctc 
      ccttgagga tgctttcgg cgaaaaata gttatcact 
      atgtacaac attgtaatt aaataaatg ggtaataaa 
      atcgcgtca ttggtgata atgaacaat aaaaattaa 
      gaaaaacgc gtcactggc tgtaaaaac aaggataag 
      aaaattcca gtacgtcac aatttgatt ggaattaga 
      attaattat ttaattacg gaaattact ataattggg 
      tcagagaca aggttaata gaggcctta aaattcggt 
      attttaatt gattgtgat tttggttta aaacgtaaa 
      tattaatca tagttgagt aaatatttt gttagccaa 
      taagtgttt ggaaatttt attgatgat gacaaatac 
      aaggtttgt gatttattt attatgttt ttaaatcac 
      atttcaagt gacaaccgg aaaatgcaa ttttcttca 
      aacaaataa atgttttag aatact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY