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Gene information
General information
TC015839 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9207948..9213542
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015839
    iBeetle screen iB_05908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015839 RA
      MGSAKAESA QNNVKNAVK PYGKVILTL KNKLLPEEK 
      LSPTPSQND GLDIETETD LRIYGCELI QTAGILLKL 
      PQVAMATGQ VLLQRFYYS KSLVRHPVD HTAMACVCL 
      ASKIEEAPR RVRDVINVF THIRQVNSN KTIQPVILD 
      VNYIQLKNL VIKAERRVL KELGFCVHI KHPHKIIVM 
      YLQVLGYEK NQKLMQYSW NYMNDSLRT DVFVRYQPE 
      TVACACIYL TARKLQLPL PKNPSWYSI FGATEAEVR 
      DIAIRILKL YNRPKPNIE QLERKVEEL CKQYHDARV 
      KARGNSGNN TPNNDSSSP NTQKTAGAH NAWGGFISR 
      SGSHIAPPV SEKRSRSRS GSHSPVSKH NHKRSKKHR 
      SRSRSPSRN HKKNHKRRS YSRSRSNSP HKTSRKGRE 
      KRRSTSPVN DREGKNDRY IDRYDKYEK YEKERYSGK 
      EERERERYD EKKERHDKR EDKYKDKEE KYREDRDKY 
      KKSKHEKDI RERSKDRRR
    CDS sequence
    • >TC015839 RA
      atgggttct gcgaaagca gaatcggcc caaaacaat 
      gtgaaaaac gctgtaaag ccctacggc aaagtaatt 
      ctaacgctg aagaacaaa ctgctgccg gaggagaag 
      ctgtctccg acgccaagc cagaacgat ggtctcgac 
      attgaaacc gagacggat ttacgcatt tacggttgt 
      gaattgatt caaacggct gggattttg ctgaaattg 
      cctcaggta gcgatggct actggacaa gtccttttg 
      caacgtttt tattattcg aagagttta gtgcgtcat 
      ccggtcgat cacacggcg atggcttgc gtttgttta 
      gcgtctaag attgaagag gctcctcga agggttcgg 
      gacgttatc aacgtcttt actcacata cgacaagtg 
      aattccaac aaaactata caacctgtg atactggac 
      gtaaactat atccaattg aaaaatctc gtaatcaaa 
      gcggagcgg agggttttg aaggagctc ggcttctgc 
      gtccacatc aagcatcca cataaaatc atcgtcatg 
      tatttgcaa gtcctgggc tacgaaaaa aaccaaaag 
      ctaatgcaa tattcgtgg aattacatg aacgactcg 
      ttgcgtact gacgttttc gttcgttac caaccggaa 
      acagtggcg tgcgcttgt atttactta acggcgcgt 
      aaattgcag ttgccttta ccgaaaaac ccgtcctgg 
      tattcgatt tttggcgcc accgaagcg gaagtcaga 
      gacatagcg attaggata ttaaaattg tacaataga 
      ccgaagcca aacatcgag caacttgag aggaaagtc 
      gaagagttg tgtaaacag tatcatgat gccagggtc 
      aaagctcgg ggtaattcc ggaaataac acccctaat 
      aacgattcg tccagtcct aatacgcaa aagacagct 
      ggggcgcat aatgcgtgg ggtgggttt attagtcgg 
      tcggggagt cacatagcg ccgcctgtt tcggagaag 
      aggtccagg tctaggtcg ggaagtcat agtccagtt 
      agtaaacat aatcacaaa aggtcgaag aagcacagg 
      tcgaggagt cgctcgccg tccaggaac cacaagaag 
      aatcacaaa aggaggagt tactcaagg tcacggagt 
      aactcgcct cataaaacg tccaggaag gggagggag 
      aagagaagg tcaacgtca cctgttaat gaccgtgag 
      ggtaaaaat gaccggtat atagataga tatgacaag 
      tatgagaaa tacgagaag gagaggtat agtgggaaa 
      gaagaaaga gagagagag aggtacgac gagaagaag 
      gaacgacat gacaaacga gaggacaaa tacaaggat 
      aaagaagaa aagtacagg gaagacaga gacaaatac 
      aagaaatcg aagcatgag aaagatata cgcgagagg 
      tcgaaggat cggcggaga taa
    mRNA sequence
    • >TC015839 RA
      cgcagtcga aaagtcaaa tcgccattt tggtgatcg 
      ttgtgtgaa cgttttgag tgtcgtaaa atcccaaaa 
      atgggttct gcgaaagca gaatcggcc caaaacaat 
      gtgaaaaac gctgtaaag ccctacggc aaagtaatt 
      ctaacgctg aagaacaaa ctgctgccg gaggagaag 
      ctgtctccg acgccaagc cagaacgat ggtctcgac 
      attgaaacc gagacggat ttacgcatt tacggttgt 
      gaattgatt caaacggct gggattttg ctgaaattg 
      cctcaggta gcgatggct actggacaa gtccttttg 
      caacgtttt tattattcg aagagttta gtgcgtcat 
      ccggtcgat cacacggcg atggcttgc gtttgttta 
      gcgtctaag attgaagag gctcctcga agggttcgg 
      gacgttatc aacgtcttt actcacata cgacaagtg 
      aattccaac aaaactata caacctgtg atactggac 
      gtaaactat atccaattg aaaaatctc gtaatcaaa 
      gcggagcgg agggttttg aaggagctc ggcttctgc 
      gtccacatc aagcatcca cataaaatc atcgtcatg 
      tatttgcaa gtcctgggc tacgaaaaa aaccaaaag 
      ctaatgcaa tattcgtgg aattacatg aacgactcg 
      ttgcgtact gacgttttc gttcgttac caaccggaa 
      acagtggcg tgcgcttgt atttactta acggcgcgt 
      aaattgcag ttgccttta ccgaaaaac ccgtcctgg 
      tattcgatt tttggcgcc accgaagcg gaagtcaga 
      gacatagcg attaggata ttaaaattg tacaataga 
      ccgaagcca aacatcgag caacttgag aggaaagtc 
      gaagagttg tgtaaacag tatcatgat gccagggtc 
      aaagctcgg ggtaattcc ggaaataac acccctaat 
      aacgattcg tccagtcct aatacgcaa aagacagct 
      ggggcgcat aatgcgtgg ggtgggttt attagtcgg 
      tcggggagt cacatagcg ccgcctgtt tcggagaag 
      aggtccagg tctaggtcg ggaagtcat agtccagtt 
      agtaaacat aatcacaaa aggtcgaag aagcacagg 
      tcgaggagt cgctcgccg tccaggaac cacaagaag 
      aatcacaaa aggaggagt tactcaagg tcacggagt 
      aactcgcct cataaaacg tccaggaag gggagggag 
      aagagaagg tcaacgtca cctgttaat gaccgtgag 
      ggtaaaaat gaccggtat atagataga tatgacaag 
      tatgagaaa tacgagaag gagaggtat agtgggaaa 
      gaagaaaga gagagagag aggtacgac gagaagaag 
      gaacgacat gacaaacga gaggacaaa tacaaggat 
      aaagaagaa aagtacagg gaagacaga gacaaatac 
      aagaaatcg aagcatgag aaagatata cgcgagagg 
      tcgaaggat cggcggaga taaaaactt aattaagaa 
      gtctagtga ttaatgttt ttaagtcag attgtagtt 
      atgcaattt taccgttag ccacgaacc ggattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY