Status
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Gene information
General information
TC033463
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015838,TC015837
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9199508..9204862
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033463
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05907

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033463 RA
      MPIEPKNRL KHDCDLRKE YQSSYHVSS PFDPWWKKC 
      LNSFRLQLS VCKNKQSKR YFRSMSSLS NERKRHFST 
      PRYKNIIHP FSSFNRVKE MTLSVLLYV LFFFESFLG 
      SFLGEEFHM KTRRMYVAK VLFVVNVIL FLDIILRFF 
      VGISNEKTR EVILEKRVI AKFYLRSYF IFDLLPIIG 
      FVVFYFTPI KNLNAKIAI YELHKLRLV RMRTVVDNA 
      FTILKKFKL KDSTQTVVV ITISVLLTL HEWSCILSL 
      IYTIRRLHH MSNRGSFVY QYYQRTIRL SQTTVTHNM 
      AERTNFSFR VYLLYMSII SCHFFGCDT SIYETKRSA 
      EMVVLSLVL INGMMYYIG MVAKVLQMF GVANISENR 
      YEELNWQVN QYMTKKKFP GALKRRIFQ YYDYKYGNK 
      FFSEQEILD SLTERLRME VLLYSCRNL ITEVKIFKG 
      LSKSAVGCI LALLKQEIY LPGEMIMSA EESSDSIYF 
      ILYGTCMIQ VIGGKELWH IEDGTEFGD AEKFSEEEK 
      IGFLYQIVA LEATEVYEL QVTDLAYCC TTYPEITFK 
      MEAMARNKA RVYNQTFAR RTQENDYLG EILDEVEVV 
      LLLGLRKAV PAQRASWGS GAAAAGASG ATKRLPEVK 
      IFKGLSKSA VGCILALLK QEIYLPGEM IMSAEESSD 
      SIYFILYGT CMIQVIGGK ELWHIEDGT EFGDAEKFS 
      EEEKIGFLY QIVALEATE VYEQVTDLA YCCTTHPEI 
      TFKMEAMAR NKARVYNQT FARRTQEND YLGEILDEL 
      NKGNILHHG IKREDFN
    CDS sequence
    • >TC033463 RA
      atgccaatc gagcccaaa aaccgcctc aaacacgac 
      tgcgacctc cgtaaagaa taccaaagc tcgtaccac 
      gtgagctct ccattcgac ccatggtgg aagaaatgc 
      ctcaactcc ttccgcctc caactcagc gtttgcaaa 
      aacaaacag tccaaacgc tacttcagg agcatgtca 
      tcgctgtca aacgaacgc aaaagacac ttctccacc 
      ccacgctac aaaaacatc atccacccc ttcagcagc 
      ttcaaccgc gtcaaggag atgaccctc tccgtcctc 
      ctctacgtc ctgttcttc ttcgagtcg ttcttgggg 
      agcttccta ggcgaagag ttccacatg aagaccagg 
      aggatgtac gtggccaag gtcctgttc gtcgtcaac 
      gttattctg ttcctcgat attattctg cgatttttt 
      gtggggatt tcgaacgaa aagacgcga gaggtgatt 
      ttggagaaa agagtcatt gcgaagttt taccttcgc 
      agttacttc atctttgat ctgttgccc attattggg 
      ttcgtggtg ttttacttc actccgatt aaaaatttg 
      aacgcgaaa attgccatt tacgagctg cataagttg 
      cgtctggtg aggatgcgg actgtggtt gataacgcg 
      tttacaatt ttgaaaaag ttcaagctc aaggactcg 
      actcagact gttgtggtg attacgatt tcggttttg 
      ttgactttg cacgaatgg tcgtgcatt ttgagtctc 
      atttatacg atcagacgg ctgcatcat atgtcaaat 
      cggggctct ttcgtttac cagtactac cagaggacc 
      attcgtttg tcccaaact acggtcact cacaacatg 
      gctgagaga acgaacttc tccttccgt gtgtacctt 
      ttgtatatg tccatcatt tcgtgtcat ttttttggc 
      tgcgacact tcaatttat gagacgaaa cgtagtgcg 
      gaaatggtg gtactatct ctagttctg atcaacgga 
      atgatgtac tacattggg atggtagcc aaggtttta 
      caaatgttt ggagtagca aacatttct gaaaaccgt 
      tacgaagag ctgaactgg caggtgaac caatacatg 
      acgaagaag aagtttcca ggtgcctta aaacgccgc 
      atcttccaa tactatgac tacaagtac ggtaacaag 
      ttcttttcc gagcaagag attcttgat tccctcacg 
      gagcgtctc cggatggaa gttcttctt tacagttgc 
      cggaatctc atcaccgaa gtgaaaatc ttcaaagga 
      ttgtccaag tctgcagtt gggtgcatt ctggcgttg 
      ctcaagcag gagatttac cttccagga gagatgata 
      atgagtgct gaggaatca tctgattcg atttacttt 
      atcctgtat ggcacttgc atgatccag gtgattggt 
      gggaaggag ttgtggcat attgaagat gggactgag 
      tttggagac gctgaaaag ttctccgaa gaggagaaa 
      atcgggttt ttgtaccag attgtggcg ttggaggcg 
      accgaagtc tacgagctg caagtgacc gatttggca 
      tattgttgc acaacttat cccgaaatt accttcaaa 
      atggaagca atggcgagg aataaagcc agggtgtac 
      aaccagacg tttgcaagg aggacgcaa gagaatgac 
      tatttgggg gagattttg gatgaggta gaggtcgtc 
      cttttattg ggtttgcgg aaggcggtg cctgcgcaa 
      agggctagt tggggtagt ggggctgcg gccgctggt 
      gcaagtggc gctaccaaa aggttgccc gaagtgaaa 
      atcttcaaa ggattgtcc aagtctgca gttgggtgc 
      attctggcg ttgctcaag caggagatt taccttcca 
      ggagagatg ataatgagt gctgaggaa tcatctgat 
      tcgatttac tttatcctg tatggcact tgcatgatc 
      caggtgatt ggtgggaag gagttgtgg catattgaa 
      gatgggact gagtttgga gatgctgaa aagttctcc 
      gaagaggag aaaatcggg tttttgtac cagattgtg 
      gcgttggag gcgaccgaa gtctacgag caagtgaca 
      gatttggca tattgttgc acgactcat cccgaaatt 
      accttcaaa atggaagca atggcgagg aataaagcc 
      agggtgtac aaccagacg tttgcaaga agaacgcaa 
      gagaatgac tatttgggg gagattttg gacgagttg 
      aataaaggg aacatttta catcatggg attaagaga 
      gaggatttc aattga
    mRNA sequence
    • >TC033463 RA
      tgtcacata ataaaaatt aggatcagt cgatcaagt 
      cgttttagt ccacacagt gtcaaaatg ccaatcgag 
      cccaaaaac cgcctcaaa cacgactgc gacctccgt 
      aaagaatac caaagctcg taccacgtg agctctcca 
      ttcgaccca tggtggaag aaatgcctc aactccttc 
      cgcctccaa ctcagcgtt tgcaaaaac aaacagtcc 
      aaacgctac ttcaggagc atgtcatcg ctgtcaaac 
      gaacgcaaa agacacttc tccacccca cgctacaaa 
      aacatcatc caccccttc agcagcttc aaccgcgtc 
      aaggagatg accctctcc gtcctcctc tacgtcctg 
      ttcttcttc gagtcgttc ttggggagc ttcctaggc 
      gaagagttc cacatgaag accaggagg atgtacgtg 
      gccaaggtc ctgttcgtc gtcaacgtt attctgttc 
      ctcgatatt attctgcga ttttttgtg gggatttcg 
      aacgaaaag acgcgagag gtgattttg gagaaaaga 
      gtcattgcg aagttttac cttcgcagt tacttcatc 
      tttgatctg ttgcccatt attgggttc gtggtgttt 
      tacttcact ccgattaaa aatttgaac gcgaaaatt 
      gccatttac gagctgcat aagttgcgt ctggtgagg 
      atgcggact gtggttgat aacgcgttt acaattttg 
      aaaaagttc aagctcaag gactcgact cagactgtt 
      gtggtgatt acgatttcg gttttgttg actttgcac 
      gaatggtcg tgcattttg agtctcatt tatacgatc 
      agacggctg catcatatg tcaaatcgg ggctctttc 
      gtttaccag tactaccag aggaccatt cgtttgtcc 
      caaactacg gtcactcac aacatggct gagagaacg 
      aacttctcc ttccgtgtg taccttttg tatatgtcc 
      atcatttcg tgtcatttt tttggctgc gacacttca 
      atttatgag acgaaacgt agtgcggaa atggtggta 
      ctatctcta gttctgatc aacggaatg atgtactac 
      attgggatg gtagccaag gttttacaa atgtttgga 
      gtagcaaac atttctgaa aaccgttac gaagagctg 
      aactggcag gtgaaccaa tacatgacg aagaagaag 
      tttccaggt gccttaaaa cgccgcatc ttccaatac 
      tatgactac aagtacggt aacaagttc ttttccgag 
      caagagatt cttgattcc ctcacggag cgtctccgg 
      atggaagtt cttctttac agttgccgg aatctcatc 
      accgaagtg aaaatcttc aaaggattg tccaagtct 
      gcagttggg tgcattctg gcgttgctc aagcaggag 
      atttacctt ccaggagag atgataatg agtgctgag 
      gaatcatct gattcgatt tactttatc ctgtatggc 
      acttgcatg atccaggtg attggtggg aaggagttg 
      tggcatatt gaagatggg actgagttt ggagacgct 
      gaaaagttc tccgaagag gagaaaatc gggtttttg 
      taccagatt gtggcgttg gaggcgacc gaagtctac 
      gagctgcaa gtgaccgat ttggcatat tgttgcaca 
      acttatccc gaaattacc ttcaaaatg gaagcaatg 
      gcgaggaat aaagccagg gtgtacaac cagacgttt 
      gcaaggagg acgcaagag aatgactat ttgggggag 
      attttggat gaggtagag gtcgtcctt ttattgggt 
      ttgcggaag gcggtgcct gcgcaaagg gctagttgg 
      ggtagtggg gctgcggcc gctggtgca agtggcgct 
      accaaaagg ttgcccgaa gtgaaaatc ttcaaagga 
      ttgtccaag tctgcagtt gggtgcatt ctggcgttg 
      ctcaagcag gagatttac cttccagga gagatgata 
      atgagtgct gaggaatca tctgattcg atttacttt 
      atcctgtat ggcacttgc atgatccag gtgattggt 
      gggaaggag ttgtggcat attgaagat gggactgag 
      tttggagat gctgaaaag ttctccgaa gaggagaaa 
      atcgggttt ttgtaccag attgtggcg ttggaggcg 
      accgaagtc tacgagcaa gtgacagat ttggcatat 
      tgttgcacg actcatccc gaaattacc ttcaaaatg 
      gaagcaatg gcgaggaat aaagccagg gtgtacaac 
      cagacgttt gcaagaaga acgcaagag aatgactat 
      ttgggggag attttggac gagttgaat aaagggaac 
      attttacat catgggatt aagagagag gatttcaat 
      tgacttgcc ggtggggct attatgatc caccttccc 
      ctggaaaaa cgtaacaag acggttttt tattagttt 
      aaaataatt ataacattt tttgtaaat aattttttt 
      tttcgcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY