Status
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Gene information
General information
TC033463
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015838,TC015837 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9199508..9204862
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033463
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05907

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033463 RA
      MPIEPKNRL KHDCDLRKE YQSSYHVSS PFDPWWKKC 
LNSFRLQLS VCKNKQSKR YFRSMSSLS NERKRHFST 
PRYKNIIHP FSSFNRVKE MTLSVLLYV LFFFESFLG 
SFLGEEFHM KTRRMYVAK VLFVVNVIL FLDIILRFF 
VGISNEKTR EVILEKRVI AKFYLRSYF IFDLLPIIG 
FVVFYFTPI KNLNAKIAI YELHKLRLV RMRTVVDNA 
FTILKKFKL KDSTQTVVV ITISVLLTL HEWSCILSL 
IYTIRRLHH MSNRGSFVY QYYQRTIRL SQTTVTHNM 
AERTNFSFR VYLLYMSII SCHFFGCDT SIYETKRSA 
EMVVLSLVL INGMMYYIG MVAKVLQMF GVANISENR 
YEELNWQVN QYMTKKKFP GALKRRIFQ YYDYKYGNK 
FFSEQEILD SLTERLRME VLLYSCRNL ITEVKIFKG 
LSKSAVGCI LALLKQEIY LPGEMIMSA EESSDSIYF 
ILYGTCMIQ VIGGKELWH IEDGTEFGD AEKFSEEEK 
IGFLYQIVA LEATEVYEL QVTDLAYCC TTYPEITFK 
MEAMARNKA RVYNQTFAR RTQENDYLG EILDEVEVV 
LLLGLRKAV PAQRASWGS GAAAAGASG ATKRLPEVK 
IFKGLSKSA VGCILALLK QEIYLPGEM IMSAEESSD 
SIYFILYGT CMIQVIGGK ELWHIEDGT EFGDAEKFS 
EEEKIGFLY QIVALEATE VYEQVTDLA YCCTTHPEI 
TFKMEAMAR NKARVYNQT FARRTQEND YLGEILDEL 
NKGNILHHG IKREDFN
    CDS sequence
    • >TC033463 RA
      atgccaatc gagcccaaa aaccgcctc aaacacgac 
tgcgacctc cgtaaagaa taccaaagc tcgtaccac 
gtgagctct ccattcgac ccatggtgg aagaaatgc 
ctcaactcc ttccgcctc caactcagc gtttgcaaa 
aacaaacag tccaaacgc tacttcagg agcatgtca 
tcgctgtca aacgaacgc aaaagacac ttctccacc 
ccacgctac aaaaacatc atccacccc ttcagcagc 
ttcaaccgc gtcaaggag atgaccctc tccgtcctc 
ctctacgtc ctgttcttc ttcgagtcg ttcttgggg 
agcttccta ggcgaagag ttccacatg aagaccagg 
aggatgtac gtggccaag gtcctgttc gtcgtcaac 
gttattctg ttcctcgat attattctg cgatttttt 
gtggggatt tcgaacgaa aagacgcga gaggtgatt 
ttggagaaa agagtcatt gcgaagttt taccttcgc 
agttacttc atctttgat ctgttgccc attattggg 
ttcgtggtg ttttacttc actccgatt aaaaatttg 
aacgcgaaa attgccatt tacgagctg cataagttg 
cgtctggtg aggatgcgg actgtggtt gataacgcg 
tttacaatt ttgaaaaag ttcaagctc aaggactcg 
actcagact gttgtggtg attacgatt tcggttttg 
ttgactttg cacgaatgg tcgtgcatt ttgagtctc 
atttatacg atcagacgg ctgcatcat atgtcaaat 
cggggctct ttcgtttac cagtactac cagaggacc 
attcgtttg tcccaaact acggtcact cacaacatg 
gctgagaga acgaacttc tccttccgt gtgtacctt 
ttgtatatg tccatcatt tcgtgtcat ttttttggc 
tgcgacact tcaatttat gagacgaaa cgtagtgcg 
gaaatggtg gtactatct ctagttctg atcaacgga 
atgatgtac tacattggg atggtagcc aaggtttta 
caaatgttt ggagtagca aacatttct gaaaaccgt 
tacgaagag ctgaactgg caggtgaac caatacatg 
acgaagaag aagtttcca ggtgcctta aaacgccgc 
atcttccaa tactatgac tacaagtac ggtaacaag 
ttcttttcc gagcaagag attcttgat tccctcacg 
gagcgtctc cggatggaa gttcttctt tacagttgc 
cggaatctc atcaccgaa gtgaaaatc ttcaaagga 
ttgtccaag tctgcagtt gggtgcatt ctggcgttg 
ctcaagcag gagatttac cttccagga gagatgata 
atgagtgct gaggaatca tctgattcg atttacttt 
atcctgtat ggcacttgc atgatccag gtgattggt 
gggaaggag ttgtggcat attgaagat gggactgag 
tttggagac gctgaaaag ttctccgaa gaggagaaa 
atcgggttt ttgtaccag attgtggcg ttggaggcg 
accgaagtc tacgagctg caagtgacc gatttggca 
tattgttgc acaacttat cccgaaatt accttcaaa 
atggaagca atggcgagg aataaagcc agggtgtac 
aaccagacg tttgcaagg aggacgcaa gagaatgac 
tatttgggg gagattttg gatgaggta gaggtcgtc 
cttttattg ggtttgcgg aaggcggtg cctgcgcaa 
agggctagt tggggtagt ggggctgcg gccgctggt 
gcaagtggc gctaccaaa aggttgccc gaagtgaaa 
atcttcaaa ggattgtcc aagtctgca gttgggtgc 
attctggcg ttgctcaag caggagatt taccttcca 
ggagagatg ataatgagt gctgaggaa tcatctgat 
tcgatttac tttatcctg tatggcact tgcatgatc 
caggtgatt ggtgggaag gagttgtgg catattgaa 
gatgggact gagtttgga gatgctgaa aagttctcc 
gaagaggag aaaatcggg tttttgtac cagattgtg 
gcgttggag gcgaccgaa gtctacgag caagtgaca 
gatttggca tattgttgc acgactcat cccgaaatt 
accttcaaa atggaagca atggcgagg aataaagcc 
agggtgtac aaccagacg tttgcaaga agaacgcaa 
gagaatgac tatttgggg gagattttg gacgagttg 
aataaaggg aacatttta catcatggg attaagaga 
gaggatttc aattga
    mRNA sequence
    • >TC033463 RA
      tgtcacata ataaaaatt aggatcagt cgatcaagt 
cgttttagt ccacacagt gtcaaaatg ccaatcgag 
cccaaaaac cgcctcaaa cacgactgc gacctccgt 
aaagaatac caaagctcg taccacgtg agctctcca 
ttcgaccca tggtggaag aaatgcctc aactccttc 
cgcctccaa ctcagcgtt tgcaaaaac aaacagtcc 
aaacgctac ttcaggagc atgtcatcg ctgtcaaac 
gaacgcaaa agacacttc tccacccca cgctacaaa 
aacatcatc caccccttc agcagcttc aaccgcgtc 
aaggagatg accctctcc gtcctcctc tacgtcctg 
ttcttcttc gagtcgttc ttggggagc ttcctaggc 
gaagagttc cacatgaag accaggagg atgtacgtg 
gccaaggtc ctgttcgtc gtcaacgtt attctgttc 
ctcgatatt attctgcga ttttttgtg gggatttcg 
aacgaaaag acgcgagag gtgattttg gagaaaaga 
gtcattgcg aagttttac cttcgcagt tacttcatc 
tttgatctg ttgcccatt attgggttc gtggtgttt 
tacttcact ccgattaaa aatttgaac gcgaaaatt 
gccatttac gagctgcat aagttgcgt ctggtgagg 
atgcggact gtggttgat aacgcgttt acaattttg 
aaaaagttc aagctcaag gactcgact cagactgtt 
gtggtgatt acgatttcg gttttgttg actttgcac 
gaatggtcg tgcattttg agtctcatt tatacgatc 
agacggctg catcatatg tcaaatcgg ggctctttc 
gtttaccag tactaccag aggaccatt cgtttgtcc 
caaactacg gtcactcac aacatggct gagagaacg 
aacttctcc ttccgtgtg taccttttg tatatgtcc 
atcatttcg tgtcatttt tttggctgc gacacttca 
atttatgag acgaaacgt agtgcggaa atggtggta 
ctatctcta gttctgatc aacggaatg atgtactac 
attgggatg gtagccaag gttttacaa atgtttgga 
gtagcaaac atttctgaa aaccgttac gaagagctg 
aactggcag gtgaaccaa tacatgacg aagaagaag 
tttccaggt gccttaaaa cgccgcatc ttccaatac 
tatgactac aagtacggt aacaagttc ttttccgag 
caagagatt cttgattcc ctcacggag cgtctccgg 
atggaagtt cttctttac agttgccgg aatctcatc 
accgaagtg aaaatcttc aaaggattg tccaagtct 
gcagttggg tgcattctg gcgttgctc aagcaggag 
atttacctt ccaggagag atgataatg agtgctgag 
gaatcatct gattcgatt tactttatc ctgtatggc 
acttgcatg atccaggtg attggtggg aaggagttg 
tggcatatt gaagatggg actgagttt ggagacgct 
gaaaagttc tccgaagag gagaaaatc gggtttttg 
taccagatt gtggcgttg gaggcgacc gaagtctac 
gagctgcaa gtgaccgat ttggcatat tgttgcaca 
acttatccc gaaattacc ttcaaaatg gaagcaatg 
gcgaggaat aaagccagg gtgtacaac cagacgttt 
gcaaggagg acgcaagag aatgactat ttgggggag 
attttggat gaggtagag gtcgtcctt ttattgggt 
ttgcggaag gcggtgcct gcgcaaagg gctagttgg 
ggtagtggg gctgcggcc gctggtgca agtggcgct 
accaaaagg ttgcccgaa gtgaaaatc ttcaaagga 
ttgtccaag tctgcagtt gggtgcatt ctggcgttg 
ctcaagcag gagatttac cttccagga gagatgata 
atgagtgct gaggaatca tctgattcg atttacttt 
atcctgtat ggcacttgc atgatccag gtgattggt 
gggaaggag ttgtggcat attgaagat gggactgag 
tttggagat gctgaaaag ttctccgaa gaggagaaa 
atcgggttt ttgtaccag attgtggcg ttggaggcg 
accgaagtc tacgagcaa gtgacagat ttggcatat 
tgttgcacg actcatccc gaaattacc ttcaaaatg 
gaagcaatg gcgaggaat aaagccagg gtgtacaac 
cagacgttt gcaagaaga acgcaagag aatgactat 
ttgggggag attttggac gagttgaat aaagggaac 
attttacat catgggatt aagagagag gatttcaat 
tgacttgcc ggtggggct attatgatc caccttccc 
ctggaaaaa cgtaacaag acggttttt tattagttt 
aaaataatt ataacattt tttgtaaat aattttttt 
tttcgcaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY